##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936325_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2031103 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.17363619668722 31.0 31.0 34.0 28.0 34.0 2 31.266950026660393 33.0 31.0 34.0 28.0 34.0 3 31.336992757137377 33.0 31.0 34.0 28.0 34.0 4 34.829849594038315 37.0 35.0 37.0 32.0 37.0 5 34.787961024133196 37.0 35.0 37.0 32.0 37.0 6 34.872322083124295 37.0 35.0 37.0 32.0 37.0 7 34.798939295545324 37.0 35.0 37.0 32.0 37.0 8 34.79278894275672 37.0 35.0 37.0 32.0 37.0 9 36.39888178984522 39.0 37.0 39.0 32.0 39.0 10-11 36.290376214303265 39.0 37.0 39.0 32.0 39.0 12-13 36.17073235576926 39.0 37.0 39.0 32.0 39.0 14-15 37.328473986794364 40.0 37.0 41.0 31.5 41.0 16-17 37.25443515173775 40.0 37.0 41.0 31.0 41.0 18-19 37.176304451325215 40.0 37.0 41.0 31.0 41.0 20-21 37.08291972391356 40.0 37.0 41.0 31.0 41.0 22-23 36.907601928607264 40.0 37.0 41.0 30.5 41.0 24-25 37.062824485021196 40.0 37.0 41.0 31.0 41.0 26-27 37.005409622259435 40.0 37.0 41.0 31.0 41.0 28-29 36.86051889047478 40.0 37.0 41.0 30.0 41.0 30-31 36.70825113251273 40.0 37.0 41.0 30.0 41.0 32-33 36.52695505840915 40.0 36.0 41.0 30.0 41.0 34-35 36.25779637960261 40.0 36.0 41.0 29.5 41.0 36-37 36.06300271330406 40.0 36.0 41.0 28.5 41.0 38-39 35.89703107129476 39.5 35.0 41.0 27.5 41.0 40-41 35.70736565304665 39.0 35.0 41.0 27.0 41.0 42-43 35.52022226346965 39.0 35.0 41.0 26.5 41.0 44-45 35.3532922751825 39.0 35.0 41.0 26.0 41.0 46-47 35.0961521892292 39.0 35.0 41.0 24.5 41.0 48-49 34.94268212887283 39.0 34.5 40.5 24.0 41.0 50-51 34.65621167414946 38.5 34.5 40.5 24.0 41.0 52-53 34.68855543022683 38.5 34.0 40.0 24.0 41.0 54-55 34.7703651661191 39.0 34.0 41.0 24.0 41.0 56-57 34.596052982049656 38.5 34.0 41.0 23.5 41.0 58-59 34.35267487665569 38.0 34.0 40.0 23.0 41.0 60-61 34.045801468463196 38.0 34.0 40.0 22.0 41.0 62-63 33.73426532283198 37.0 33.5 40.0 22.0 41.0 64-65 33.39589277353241 37.0 33.0 40.0 20.5 41.0 66-67 33.024133931169416 36.0 33.0 39.0 19.5 41.0 68-69 32.632572301847816 36.0 32.5 39.0 18.0 41.0 70-71 32.21232995077059 35.0 32.0 39.0 16.0 40.0 72-73 31.794960176810335 35.0 32.0 37.5 12.5 40.0 74-75 31.36544872416613 35.0 31.0 37.0 8.0 39.0 76-77 30.953546422805736 35.0 31.0 36.5 7.0 39.0 78-79 30.547540178907717 35.0 31.0 36.0 4.0 38.5 80-81 30.133272167881195 34.5 30.5 35.5 2.0 37.0 82-83 29.77221785404285 34.0 30.0 35.0 2.0 37.0 84-85 29.44244334236127 34.0 30.0 35.0 2.0 36.5 86-87 29.09760164797157 34.0 29.5 35.0 2.0 36.0 88-89 28.746951040887637 34.0 29.0 35.0 2.0 36.0 90-91 28.371916392226293 34.0 29.0 35.0 2.0 35.0 92-93 28.022479411433096 34.0 29.0 35.0 2.0 35.0 94-95 27.60283944241134 34.0 27.0 35.0 2.0 35.0 96-97 27.1243189045558 34.0 26.5 35.0 2.0 35.0 98-99 26.56297464973465 33.5 25.5 35.0 2.0 35.0 100-101 23.30958326584127 29.0 13.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4123929516508973 1101 2 0.426057776815135 1101 3 0.49113228566046274 1101 4 0.2556977971010639 1101 5 0.5746671988797232 1101 6 0.4762403312797545 1101 7 0.6357041356677797 1101 8 0.37806638256405023 1101 9 0.48653134069444093 1101 10-11 0.7309182114854238 1101 12-13 0.6324341734815633 1101 14-15 0.7939729782734233 1101 16-17 0.7855289076598737 1101 18-19 0.833826775063244 1101 20-21 0.909932750649304 1101 22-23 0.976801154983086 1101 24-25 0.7748584861170897 1101 26-27 0.7760865959665466 1101 28-29 0.8916610099207603 1101 30-31 0.8182003012641346 1101 32-33 0.9335951883917275 1101 34-35 0.7949690582394737 1101 36-37 0.786721306097995 1101 38-39 0.9779296324780375 1101 40-41 0.8334908192185537 1101 42-43 0.7096413389496732 1101 44-45 0.7087056373191842 1101 46-47 0.6588894827159777 1101 48-49 0.7640089687860581 1101 50-51 0.8841732938622826 1101 52-53 0.9460617983405015 1101 54-55 0.9154436268818671 1101 56-57 0.8764000135228613 1101 58-59 0.8960823162064173 1101 60-61 0.6021157853257151 1101 62-63 0.5236823622400806 1101 64-65 0.45542037978910344 1101 66-67 0.5549149616166957 1101 68-69 0.6046602886262349 1101 70-71 0.7444443313561706 1101 72-73 0.75930485470683 1101 74-75 0.5402651546520616 1101 76-77 0.5939218443110015 1101 78-79 0.6922634072880065 1101 80-81 0.6503073741467205 1101 82-83 0.8040249666937669 1101 84-85 0.9700714068700194 1101 86-87 0.8197571467086746 1101 88-89 0.7948370175492379 1101 90-91 0.8165702847811822 1101 92-93 0.9400392574643774 1101 94-95 1.1789884781135456 1101 96-97 1.2752092299069382 1101 98-99 1.5106256053094178 1101 100-101 1.4423183673745008 1102 1 0.5403457101690705 1102 2 0.4737110322488185 1102 3 0.44872624038593756 1102 4 0.4248780192265471 1102 5 0.573164440892441 1102 6 0.5186334360461089 1102 7 0.45979219483045597 1102 8 0.4660366207224911 1102 9 0.5954419755358629 1102 10-11 0.7136471124845585 1102 12-13 0.7462052963725938 1102 14-15 0.8735196092287154 1102 16-17 0.849650286972043 1102 18-19 0.8252110510849491 1102 20-21 0.6964933361177046 1102 22-23 0.8506149417341291 1102 24-25 0.8512944179929534 1102 26-27 0.9281647752171409 1102 28-29 0.9781027749795257 1102 30-31 0.8595850418169064 1102 32-33 0.680001708815162 1102 34-35 0.6697372904200591 1102 36-37 0.7452182760282327 1102 38-39 0.8054764572091386 1102 40-41 0.830334662456579 1102 42-43 0.6115443236281095 1102 44-45 0.6509740486760407 1102 46-47 0.8450532922954039 1102 48-49 0.7435314441260985 1102 50-51 0.6571105104983346 1102 52-53 0.6538802211115495 1102 54-55 0.6940816350788452 1102 56-57 0.752877388205647 1102 58-59 0.727045766902414 1102 60-61 0.7657972869024618 1102 62-63 0.8789094316596007 1102 64-65 0.8068436034223154 1102 66-67 0.7416370379909125 1102 68-69 0.9010291156720243 1102 70-71 0.8876760142327598 1102 72-73 0.8515686871146393 1102 74-75 0.9254330759950093 1102 76-77 0.8881997838635662 1102 78-79 0.8511214173962038 1102 80-81 0.8530118076311801 1102 82-83 0.9060806161878219 1102 84-85 0.858799792903298 1102 86-87 0.7156542424604702 1102 88-89 0.6836294425075629 1102 90-91 0.7356227608644694 1102 92-93 0.8850356274859301 1102 94-95 0.8578963124962762 1102 96-97 0.8665262484669469 1102 98-99 1.0055159295255542 1102 100-101 0.9478625456057834 1103 1 0.5138750187181742 1103 2 0.4870436678938397 1103 3 0.563332825644494 1103 4 0.4800669402703761 1103 5 0.5398295617826037 1103 6 0.4899763064026672 1103 7 0.5315182088702812 1103 8 0.6201084466897839 1103 9 0.6192502758543341 1103 10-11 0.6904883516291349 1103 12-13 0.7515543619506957 1103 14-15 0.8167082495261084 1103 16-17 0.869799640315243 1103 18-19 0.7813437940152141 1103 20-21 0.827876714174117 1103 22-23 0.7291294421621402 1103 24-25 0.8632178381362579 1103 26-27 0.8763250714046222 1103 28-29 0.9100056279398387 1103 30-31 0.7595072412145498 1103 32-33 0.8893537924174453 1103 34-35 0.6852933375603953 1103 36-37 0.7884632536123846 1103 38-39 0.7907342927293257 1103 40-41 0.8150798850671919 1103 42-43 0.8542814196321089 1103 44-45 0.8958471362476246 1103 46-47 0.8779861650708938 1103 48-49 0.9056037601463203 1103 50-51 0.9370476057503367 1103 52-53 0.9342030293780255 1103 54-55 0.986819483082229 1103 56-57 0.9126604927849442 1103 58-59 0.9619296393984129 1103 60-61 1.0470221810898792 1103 62-63 0.9889780140029103 1103 64-65 0.9341000715569763 1103 66-67 0.8982637818461399 1103 68-69 0.7026059330351941 1103 70-71 0.800977849236574 1103 72-73 0.7883631393645665 1103 74-75 0.7163377688471257 1103 76-77 0.6200339855472912 1103 78-79 0.8131360487974426 1103 80-81 0.8601775783741452 1103 82-83 0.945569200210592 1103 84-85 0.9428128501191679 1103 86-87 0.9530190862388679 1103 88-89 0.9236446140950179 1103 90-91 0.9563948950261683 1103 92-93 0.8329071872454392 1103 94-95 0.7388844208794403 1103 96-97 0.9408794036683368 1103 98-99 0.8301938544584679 1103 100-101 0.8648361742409172 1104 1 0.6109139598796389 1104 2 0.48863310305567964 1104 3 0.5152548648309399 1104 4 0.6142662868359139 1104 5 0.5813400648094031 1104 6 0.5079604709190164 1104 7 0.6345248903847605 1104 8 0.4969259952313294 1104 9 0.6309343412762729 1104 10-11 0.603090880008402 1104 12-13 0.6805221856999992 1104 14-15 0.745569375081665 1104 16-17 0.7464982265028581 1104 18-19 0.6541399244273833 1104 20-21 0.765358317933817 1104 22-23 0.911888117829065 1104 24-25 0.7853937699671505 1104 26-27 0.6818872359798434 1104 28-29 0.6820995125389899 1104 30-31 0.7141280088528674 1104 32-33 0.7830725801297334 1104 34-35 0.8549508240943453 1104 36-37 0.9076212209014116 1104 38-39 0.6695907579040181 1104 40-41 0.7185793405384828 1104 42-43 0.7875754258917382 1104 44-45 0.90701365410915 1104 46-47 0.8209473956076749 1104 48-49 0.873130716770433 1104 50-51 0.9077166402175365 1104 52-53 0.8226267659242481 1104 54-55 0.9302725064430035 1104 56-57 0.9516470316427146 1104 58-59 0.9384909896334008 1104 60-61 0.9234055783603665 1104 62-63 0.9772645145964916 1104 64-65 0.8716926659664779 1104 66-67 0.9262174821885552 1104 68-69 0.7961759564842694 1104 70-71 0.7917686913428739 1104 72-73 0.9078326163226542 1104 74-75 0.7751063981064732 1104 76-77 0.8189641828531045 1104 78-79 0.7495347272115396 1104 80-81 0.8087392376659643 1104 82-83 0.8763576385414922 1104 84-85 0.676157507360422 1104 86-87 0.8490006115079396 1104 88-89 1.0864703501899164 1104 90-91 1.1834480380851495 1104 92-93 1.1092622543058681 1104 94-95 0.8751821652550973 1104 96-97 1.0817909251109974 1104 98-99 1.2345977917444593 1104 100-101 1.3514099848626593 1105 1 0.35798732157534374 1105 2 0.37461919857587134 1105 3 0.3906805362361965 1105 4 0.278643839685202 1105 5 0.3666920160588205 1105 6 0.30259583206759544 1105 7 0.4043321708986767 1105 8 0.42422108338422504 1105 9 0.4461163069584302 1105 10-11 0.5005152732202944 1105 12-13 0.5588456063529605 1105 14-15 0.4859387069308525 1105 16-17 0.5213546066215287 1105 18-19 0.42662858105087764 1105 20-21 0.48305858394896717 1105 22-23 0.594709269806728 1105 24-25 0.5210429837131514 1105 26-27 0.48095205594796653 1105 28-29 0.5039247820915804 1105 30-31 0.6556146986622196 1105 32-33 0.6306150057728459 1105 34-35 0.4557712733014867 1105 36-37 0.5578460838847477 1105 38-39 0.6100126796276442 1105 40-41 0.6106462876844745 1105 42-43 0.6855463528885011 1105 44-45 0.691351540515015 1105 46-47 0.7366603303584967 1105 48-49 0.6651884665841266 1105 50-51 0.7585925092475208 1105 52-53 0.8509905133419124 1105 54-55 0.9492490791868207 1105 56-57 0.9358287992284815 1105 58-59 0.974706664717587 1105 60-61 0.8542192356913532 1105 62-63 0.6807991201488548 1105 64-65 0.8135997210278418 1105 66-67 0.738761700132315 1105 68-69 0.7234963962582484 1105 70-71 0.7615743210535584 1105 72-73 0.9118340454466001 1105 74-75 0.8804736310772583 1105 76-77 1.0355725604436863 1105 78-79 0.9485750209601811 1105 80-81 0.8741051499941612 1105 82-83 0.8883542614697326 1105 84-85 0.8554541944341594 1105 86-87 1.0086769125767177 1105 88-89 0.9205876185350554 1105 90-91 1.109865252661269 1105 92-93 0.9959151564179543 1105 94-95 0.8577602954138577 1105 96-97 0.8800780988415262 1105 98-99 0.9059600109899968 1105 100-101 0.937494067346222 1106 1 0.1736417345256953 1106 2 0.1884733853280558 1106 3 0.2692599869332284 1106 4 0.35022806129175166 1106 5 0.24060514288202484 1106 6 0.24776730178061257 1106 7 0.31356649122730573 1106 8 0.2931197373585306 1106 9 0.32426557519720944 1106 10-11 0.2603929784928809 1106 12-13 0.24551364306336865 1106 14-15 0.2144732788288266 1106 16-17 0.31306496809882844 1106 18-19 0.3162771958408115 1106 20-21 0.25143013755602084 1106 22-23 0.4104128746567923 1106 24-25 0.2741226221569164 1106 26-27 0.22443701873125121 1106 28-29 0.31153582611000985 1106 30-31 0.35521301649658454 1106 32-33 0.4280007593476398 1106 34-35 0.2402457741376054 1106 36-37 0.26428630430955735 1106 38-39 0.2028549904121988 1106 40-41 0.23127962564100812 1106 42-43 0.2828563875849994 1106 44-45 0.27594652626768834 1106 46-47 0.3270517851194299 1106 48-49 0.34805984710335025 1106 50-51 0.3867764514328016 1106 52-53 0.20572443731632717 1106 54-55 0.3377410500539355 1106 56-57 0.4664217913701876 1106 58-59 0.42602514858095475 1106 60-61 0.5356414989585048 1106 62-63 0.30680353698945595 1106 64-65 0.14549605889528294 1106 66-67 0.3696351283876922 1106 68-69 0.48679723756658433 1106 70-71 0.40992849258303465 1106 72-73 0.5572422000822534 1106 74-75 0.44618386062442283 1106 76-77 0.47424661181524996 1106 78-79 0.39633773389486393 1106 80-81 0.4957768739827806 1106 82-83 0.5558803063958919 1106 84-85 0.5998205238332659 1106 86-87 0.42665132264039585 1106 88-89 0.3120129207058149 1106 90-91 0.38856541379645293 1106 92-93 0.31067608096631716 1106 94-95 0.2584136596887525 1106 96-97 0.29463712517455676 1106 98-99 0.22925777392845248 1106 100-101 0.22663601966418767 1107 1 0.16454899526656774 1107 2 0.2495335514290069 1107 3 0.27231141282208426 1107 4 0.2416892556115826 1107 5 0.27306225720543864 1107 6 0.4201838535023228 1107 7 0.24637797886556 1107 8 0.40670557156878573 1107 9 0.40885011622178524 1107 10-11 0.3384790636919277 1107 12-13 0.3770414395224506 1107 14-15 0.5377798077901232 1107 16-17 0.3565994353212787 1107 18-19 0.4802585901428813 1107 20-21 0.4708919226445616 1107 22-23 0.4067974932197629 1107 24-25 0.5768981801564266 1107 26-27 0.5571932097148249 1107 28-29 0.5082978509346034 1107 30-31 0.46397555824379566 1107 32-33 0.5799351312734657 1107 34-35 0.44709350438136397 1107 36-37 0.434843077446466 1107 38-39 0.46808552647326707 1107 40-41 0.44410757040764537 1107 42-43 0.10220467826782453 1107 44-45 0.35057107604830406 1107 46-47 0.479764617899356 1107 48-49 0.32158954070362 1107 50-51 0.5212361134462853 1107 52-53 0.540070517012083 1107 54-55 0.5839900643405258 1107 56-57 0.5722959840082567 1107 58-59 0.5913379702839663 1107 60-61 0.6106146198612592 1107 62-63 0.6674067364591636 1107 64-65 0.37624442746814424 1107 66-67 0.35398898829638625 1107 68-69 0.5501413577036303 1107 70-71 0.4619899852762259 1107 72-73 0.4368176905653378 1107 74-75 0.4724792221230061 1107 76-77 0.4012181045875671 1107 78-79 0.44084890355537354 1107 80-81 0.4672195364507701 1107 82-83 0.7188806026593682 1107 84-85 0.7090692189992538 1107 86-87 0.395314646892416 1107 88-89 0.6059955194024305 1107 90-91 0.7708169458830163 1107 92-93 0.4236100476177782 1107 94-95 0.3568592874288612 1107 96-97 0.6825431577846395 1107 98-99 0.6387330183458211 1107 100-101 0.6807270490347115 1108 1 0.12566677930735182 1108 2 0.13141483467549975 1108 3 0.17202149134178413 1108 4 0.16733531780628397 1108 5 0.36281774721092575 1108 6 0.2015963657789257 1108 7 0.22332408311937257 1108 8 0.16390068372735556 1108 9 0.34398783594414084 1108 10-11 0.15477270142316257 1108 12-13 0.20667635071819745 1108 14-15 0.17362034704520823 1108 16-17 0.4530171390061426 1108 18-19 0.3359246655112429 1108 20-21 0.38044447884457355 1108 22-23 0.447154024202284 1108 24-25 0.23982978440546532 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2315 96-97 -1.7819034689461866 2315 98-99 -1.9751247184049738 2315 100-101 -1.9943598605204294 2316 1 -1.3146227949041993 2316 2 -1.3104516755297553 2316 3 -1.382270723942618 2316 4 -1.1652247532305537 2316 5 -1.4267533183337662 2316 6 -1.2774122959647656 2316 7 -1.3483277986253697 2316 8 -1.561662510507901 2316 9 -1.6229932479024498 2316 10-11 -1.5541459530611164 2316 12-13 -1.609040195626804 2316 14-15 -1.9905776217353974 2316 16-17 -1.9747021711059958 2316 18-19 -1.926795623795556 2316 20-21 -1.9434741009220389 2316 22-23 -2.0290523318328937 2316 24-25 -1.9092480816265507 2316 26-27 -1.895329527799987 2316 28-29 -2.1944503207605734 2316 30-31 -1.9681756932357857 2316 32-33 -2.110843884241696 2316 34-35 -2.2031428165675138 2316 36-37 -2.0475467070345488 2316 38-39 -2.0747650762676813 2316 40-41 -1.796253735848424 2316 42-43 -2.085144246132465 2316 44-45 -2.344032730156812 2316 46-47 -2.235487180911427 2316 48-49 -2.1831083601452406 2316 50-51 -2.0903116250335856 2316 52-53 -2.2835350983050375 2316 54-55 -2.158363065128313 2316 56-57 -2.23152000184502 2316 58-59 -2.4371298450018344 2316 60-61 -2.2931096959313635 2316 62-63 -2.396236726643579 2316 64-65 -2.1907152711028246 2316 66-67 -2.2407031265169515 2316 68-69 -2.337615516900321 2316 70-71 -2.132816961089304 2316 72-73 -2.146134931172373 2316 74-75 -2.056706859642265 2316 76-77 -2.4231641146736855 2316 78-79 -2.5886566246242246 2316 80-81 -2.384410507733893 2316 82-83 -2.4672310903806043 2316 84-85 -2.5461680323129094 2316 86-87 -2.732310484800614 2316 88-89 -2.682503412089435 2316 90-91 -2.848496940472131 2316 92-93 -2.7367962934260817 2316 94-95 -2.7444970694680357 2316 96-97 -3.0695570993773167 2316 98-99 -2.868537369513742 2316 100-101 -2.689511236737321 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31439.0 3 6425.0 4 5285.0 5 4070.0 6 3995.0 7 4238.0 8 4855.0 9 5691.0 10 6414.0 11 7379.0 12 8880.0 13 11692.0 14 11000.0 15 10222.0 16 11054.0 17 13281.0 18 12478.0 19 10824.0 20 11192.0 21 11543.0 22 12334.0 23 13412.0 24 15559.0 25 18046.0 26 21063.0 27 25126.0 28 29812.0 29 35593.0 30 43384.0 31 53082.0 32 66656.0 33 85522.0 34 116928.0 35 169312.0 36 269277.0 37 418581.0 38 380980.0 39 64424.0 40 55.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.483490891322425 17.550360761164683 12.398677537445472 24.567470810067423 2 15.902962703264842 18.800914691793775 39.77121872242531 25.52490388251607 3 20.236289795243085 22.60150199714806 27.803590139524626 29.358618068084237 4 12.752069436156196 14.653656250061548 35.555453798840766 37.03882051494149 5 14.432480047347696 36.93184364033673 33.67142096760454 14.964255344711027 6 35.28102623036219 34.14910237340144 16.527841171225333 14.042030225011043 7 31.387341468002116 30.75020999735103 19.269337235558435 18.59311129908842 8 27.65353060567296 35.29428853640113 18.494134121949994 18.558046735975907 9 26.959133265970586 15.827045158647152 18.928489124461542 38.28533245092072 10-11 26.377630689143377 25.636240950188093 26.481296255312216 21.504832105356318 12-13 26.55154322803713 22.500894298474854 27.26592337517723 23.681639098310793 14-15 23.155876190079933 24.280794237638567 24.693492329891598 27.8698372423899 16-17 23.796729757219843 27.54823385271769 25.38824561217764 23.26679077788483 18-19 23.623467647267372 26.509185253173033 26.886733922113937 22.980613177445658 20-21 24.089731223852436 25.805223087304363 27.010995947358495 23.094049741484703 22-23 23.72626103518111 25.733385001952346 26.83620145049636 23.704152512370186 24-25 23.598976292413592 25.882891161387096 27.030622586373482 23.487509959825832 26-27 23.55021477570169 26.199238191275438 26.81791235260636 23.432634680416513 28-29 23.300879558603057 26.301247159586836 27.030244475582606 23.3676288062275 30-31 23.571089154739894 25.953185821316282 26.955724236075035 23.520000787868792 32-33 23.356806298061844 25.968992248062015 27.0880819677701 23.586119486106043 34-35 23.70272409613307 26.176567792395378 26.777726521155447 23.342981590316104 36-37 23.798044402234332 26.023523068609155 26.714721889364164 23.463710639792346 38-39 23.484273662217614 26.085519937636764 26.952140372675725 23.478066027469897 40-41 23.662720830836985 26.0188187031332 26.991218960988718 23.327241505041098 42-43 23.633130225833938 25.97163255639665 26.754117904583584 23.641119313185825 44-45 23.58824130396224 26.162512396759634 26.777594525214926 23.471651774063197 46-47 23.65400477276427 26.41004606019343 26.438966656457456 23.49698251058484 48-49 23.844741124284145 26.289764347600837 26.430319040101264 23.43517548801376 50-51 23.57674049752007 26.357255882487575 26.533851134928227 23.532152485064127 52-53 23.812060339054003 26.355175656554984 26.352577863887046 23.480186140503967 54-55 23.78729725617864 26.228497991888393 26.40181280466461 23.58239194726836 56-57 23.61767216653313 26.558044695680955 26.372668167852087 23.451614969933825 58-59 23.786135067511495 26.227432782250236 26.417753723119393 23.56867842711888 60-61 23.704699354501667 26.317241058601432 26.602070660497958 23.375988926398943 62-63 23.59360688691545 26.336505386091662 26.59683747832318 23.473050248669708 64-65 23.50880309172587 26.3317198022004 26.50580502603362 23.653672080040117 66-67 23.538175709766072 26.488287796317074 26.345854776192933 23.62768171772392 68-69 23.522621654337193 26.277079548791495 26.568603172867522 23.631695624003793 70-71 23.540795302486526 26.193905995315347 26.54834664443889 23.716952057759233 72-73 23.623642442015065 26.31203086098334 26.409912322254364 23.654414374747226 74-75 23.472910876568676 26.580996719033813 26.18310967907292 23.762982725324598 76-77 23.619406464814276 26.47529764945304 26.247597147472995 23.657698738259693 78-79 23.630936935470583 26.446376461700616 26.31562022511544 23.60706637771336 80-81 23.469650018070396 26.545105618446698 26.34639308174623 23.638851281736677 82-83 23.66142371164011 26.597871022514756 26.306343790878127 23.434361474966998 84-85 23.65987510250426 26.41605535168082 26.115450406492 23.80861913932292 86-87 23.489402440110428 26.328628254222696 26.467356349135667 23.7146129565312 88-89 23.621034620198625 26.503424512647427 26.205296582860143 23.670244284293805 90-91 23.61135720444136 26.611883816671632 26.22382900544529 23.55292997344172 92-93 23.61991653358526 26.459239478733288 26.274773060529235 23.64607092715222 94-95 23.590293380854362 26.335508604570222 26.396732170522597 23.67746584405282 96-97 23.58353055809042 26.286486580060885 26.333672139499107 23.796310722349585 98-99 23.432602675432946 26.3281157849159 26.2414190373298 23.99786250232135 100-101 23.57533576827 26.356742704649307 26.018631247067926 24.04929028001277 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 45.0 1 39.0 2 49.0 3 90.5 4 102.0 5 93.0 6 183.0 7 367.5 8 583.0 9 717.0 10 723.5 11 795.0 12 956.5 13 1127.0 14 1306.5 15 1477.5 16 1630.0 17 1764.5 18 1857.0 19 1901.5 20 1977.0 21 2105.0 22 2261.5 23 2654.5 24 3283.0 25 4243.5 26 5709.0 27 7468.0 28 9430.5 29 12196.5 30 15518.5 31 18935.5 32 22812.0 33 26461.0 34 30047.0 35 34295.5 36 39568.5 37 44239.5 38 47885.5 39 51312.5 40 53732.0 41 57196.5 42 60941.0 43 63808.5 44 68036.0 45 72619.0 46 75174.0 47 77010.5 48 79872.5 49 80993.0 50 79821.5 51 79037.0 52 80109.5 53 80715.0 54 80032.5 55 76343.5 56 69854.0 57 63990.5 58 59063.0 59 53520.5 60 46977.5 61 39918.5 62 32734.5 63 26212.5 64 20130.0 65 14916.0 66 10531.0 67 8102.5 68 6071.0 69 4112.5 70 3060.0 71 2164.0 72 1572.0 73 1261.0 74 1010.5 75 724.5 76 482.5 77 272.5 78 159.0 79 106.0 80 73.0 81 58.0 82 41.5 83 31.0 84 27.0 85 26.0 86 21.5 87 23.0 88 22.0 89 16.5 90 17.5 91 18.0 92 21.0 93 23.5 94 17.5 95 20.0 96 22.5 97 19.5 98 14.0 99 6.0 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046821849999729215 2 0.01255475473178859 3 0.010191506782275444 4 0.005661948212375246 5 0.008369836487859059 6 0.006695869190287249 7 0.006154291535190486 8 0.010093038117712396 9 0.015114940010427833 10-11 0.002584802444780004 12-13 0.008936031309096584 14-15 0.009822249290164014 16-17 0.019570647081905743 18-19 0.011767005415284207 20-21 0.004037215247084959 22-23 0.010437678443683063 24-25 0.021909277865278128 26-27 0.00723744684538401 28-29 0.01814285144574155 30-31 0.015164174342709356 32-33 0.012603989064070113 34-35 0.2633298262077305 36-37 0.4662737438721719 38-39 0.46275348911404296 40-41 0.48328420567543845 42-43 0.4724280354073624 44-45 0.4878876157437609 46-47 0.49490350809387806 48-49 0.5012793541243353 50-51 0.5110769862483586 52-53 0.5000484958172973 54-55 0.4886753650602653 56-57 0.48306265118017155 58-59 0.48153638687944433 60-61 0.47850847544413055 62-63 0.47493898635372017 64-65 0.49069397268380777 66-67 0.4653136743926822 68-69 0.49222023698453504 70-71 0.4730434645608814 72-73 0.48153638687944433 74-75 0.49490350809387806 76-77 0.4828657138510455 78-79 0.4817333242085704 80-81 0.4845396811486173 82-83 0.48500740730529174 84-85 0.48473661847774335 86-87 0.4865829059383005 88-89 0.5001469644818604 90-91 0.5239025298076956 92-93 0.5125293990506636 94-95 0.4836042288352683 96-97 0.45778082155360905 98-99 0.44864785291538634 100-101 0.4571161580678084 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2031103.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.89428269504684 #Duplication Level Percentage of deduplicated Percentage of total 1 75.99313080211245 43.23574666737057 2 13.289224484649658 15.121617892551917 3 4.546036669684405 7.759304862812214 4 2.042244263132515 4.647680897559956 5 1.1492960311566576 3.2694186648461123 6 0.7006539392111751 2.3917921973327254 7 0.45798309386139957 1.8239633728191849 8 0.3050405744394008 1.3884051740491798 9 0.2306542303046239 1.181061628538374 >10 1.1726412270664233 12.004163538952426 >50 0.0774318768599469 3.0075323689365936 >100 0.033841612618511194 3.2720018317554542 >500 0.001387577068938664 0.5183739327727599 >1k 4.3361783404332943E-4 0.3789369697025294 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2430 0.11963942744410304 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.923433228152388E-5 0.0 0.0 0.0 0.0 3 4.923433228152388E-5 0.0 0.0 0.0 0.0 4 4.923433228152388E-5 0.0 0.0 0.0 0.0 5 4.923433228152388E-5 0.0 0.0 0.0 0.0 6 4.923433228152388E-5 0.0 0.0 0.0 0.0 7 9.846866456304776E-5 0.0 0.0 0.0 0.0 8 9.846866456304776E-5 0.0 0.0 0.0 0.0 9 9.846866456304776E-5 0.0 0.0 0.0 0.0 10-11 9.846866456304776E-5 0.0 0.0 0.0 0.0 12-13 1.9693732912609552E-4 0.0 0.0 2.461716614076194E-5 0.0 14-15 2.954059936891433E-4 0.0 0.0 4.923433228152388E-5 2.461716614076194E-5 16-17 2.954059936891433E-4 0.0 0.0 4.923433228152388E-5 4.923433228152388E-5 18-19 2.954059936891433E-4 0.0 0.0 4.923433228152388E-5 4.923433228152388E-5 20-21 2.954059936891433E-4 0.0 0.0 9.846866456304776E-5 4.923433228152388E-5 22-23 2.954059936891433E-4 0.0 0.0 1.4770299684457164E-4 4.923433228152388E-5 24-25 3.446403259706672E-4 0.0 0.0 3.4464032597066713E-4 4.923433228152388E-5 26-27 3.446403259706672E-4 0.0 0.0 4.4310899053371495E-4 4.923433228152388E-5 28-29 3.9387465825219104E-4 0.0 0.0 5.908119873782867E-4 4.923433228152388E-5 30-31 3.9387465825219104E-4 0.0 0.0 0.002190927786527813 4.923433228152388E-5 32-33 3.9387465825219104E-4 0.0 0.0 0.005046519058856198 4.923433228152388E-5 34-35 5.169604889560008E-4 0.0 0.0 0.008246750657155251 4.923433228152388E-5 36-37 6.400463196598105E-4 0.0 0.0 0.015213408674990879 4.923433228152388E-5 38-39 7.877493165043821E-4 0.0 0.0 0.02769431190835718 4.923433228152388E-5 40-41 7.877493165043821E-4 0.0 0.0 0.04103681595665015 4.923433228152388E-5 42-43 7.877493165043821E-4 0.0 0.0 0.05386235951598713 4.923433228152388E-5 44-45 8.862179810674299E-4 0.0 0.0 0.06905115102483725 4.923433228152388E-5 46-47 0.0010339209779120014 0.0 0.0 0.08985265641378108 4.923433228152388E-5 48-49 0.0011323896424750492 0.0 0.0 0.11257430076170435 4.923433228152388E-5 50-51 0.0011323896424750492 0.0 0.0 0.13852079387406746 4.923433228152388E-5 52-53 0.0011323896424750492 0.0 0.0 0.16491039597696422 4.923433228152388E-5 54-55 0.0011323896424750492 0.0 0.0 0.19230930189163228 4.923433228152388E-5 56-57 0.0011323896424750492 0.0 0.0 0.22559171051394245 4.923433228152388E-5 58-59 0.0011816239747565731 0.0 0.0 0.2644868330163463 4.923433228152388E-5 60-61 0.0011816239747565731 0.0 0.0 0.3082315372484803 4.923433228152388E-5 62-63 0.0011816239747565731 0.0 0.0 0.353034779624667 4.923433228152388E-5 64-65 0.0011816239747565731 0.0 0.0 0.4011613394298566 4.923433228152388E-5 66-67 0.0011816239747565731 0.0 0.0 0.456131471422178 4.923433228152388E-5 68-69 0.001280092639319621 0.0 0.0 0.5188560107488395 4.923433228152388E-5 70-71 0.0013539441377419068 0.0 0.0 0.5899011522310784 4.923433228152388E-5 72-73 0.0014524128023049544 0.0 0.0 0.6657958754430475 4.923433228152388E-5 74-75 0.0014770299684457163 0.0 0.0 0.7423552621408172 4.923433228152388E-5 76-77 0.0014770299684457163 0.0 0.0 0.8284414921350616 4.923433228152388E-5 78-79 0.0014770299684457163 0.0 0.0 0.9270086253626724 4.923433228152388E-5 80-81 0.0018955217928386693 0.0 0.0 1.0422169629014384 4.923433228152388E-5 82-83 0.0019201389589794313 0.0 0.0 1.1637026778060984 4.923433228152388E-5 84-85 0.002043224789683241 0.0 0.0 1.2816927551187705 4.923433228152388E-5 86-87 0.0021663106203870506 0.0 0.0 1.409332761558621 4.923433228152388E-5 88-89 0.0022155449526685745 0.0 0.0 1.5554356426040432 4.923433228152388E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2800 0.0 37.702217 1 GTATCAA 4195 0.0 33.966793 1 ATCAACG 4970 0.0 28.28359 3 TCAACGC 5100 0.0 27.561956 4 GTACATG 6870 0.0 27.28717 1 CAACGCA 5135 0.0 27.189758 5 TATCAAC 5310 0.0 26.651508 2 TACATGG 7095 0.0 25.883589 2 AACGCAG 5505 0.0 25.79089 6 ACATGGG 6945 0.0 25.692335 3 TAACGCG 50 0.0016409735 23.783184 76-77 TACCTGG 1875 0.0 23.476149 2 GTACCTG 1940 0.0 23.425537 1 CATGGGG 4065 0.0 22.238045 4 ACGCAGA 6565 0.0 21.482985 7 CGCAGAG 6640 0.0 20.955742 8 AGTACAT 4680 0.0 20.530302 2 GAGTACA 5160 0.0 20.275084 1 GTGTAGC 1270 0.0 19.75569 1 GTATAGG 560 0.0 18.597506 1 >>END_MODULE