FastQCFastQC Report
Thu 26 May 2016
SRR936325_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936325_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031103
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25030.12323353370065426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30400.035.534421
GTATCAA46250.033.9546431
GTACATG71900.029.5852761
TACATGG71600.029.4241072
ATCAACG53750.028.4200743
TCAACGC54700.027.9264894
GTACCTG22050.027.840571
CAACGCA55150.027.7846435
ACATGGG71950.027.4290223
TACCTGG21000.027.1141052
TATCAAC57350.026.8067952
AACGCAG58450.026.4594546
ACGCAGA68300.022.5046427
CATGGGG42700.022.4425014
CGCAGAG69300.022.1114438
TGCACCG6600.021.5637915
GCACCGT6650.021.4016556
GAGTACA53200.019.7686751
TATACTG9900.019.6470095
AGTACAT50350.018.376742
Produced by FastQC (version 0.11.5)