##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936325_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2031103 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.42260781457169 34.0 31.0 34.0 31.0 34.0 2 32.55867181526491 34.0 31.0 34.0 31.0 34.0 3 32.64539710689217 34.0 31.0 34.0 31.0 34.0 4 36.15045765773572 37.0 35.0 37.0 35.0 37.0 5 36.1044905157444 37.0 35.0 37.0 35.0 37.0 6 36.136946772271024 37.0 36.0 37.0 35.0 37.0 7 36.078317544703545 37.0 36.0 37.0 35.0 37.0 8 36.05237991377099 37.0 35.0 37.0 35.0 37.0 9 37.81616638841063 39.0 38.0 39.0 35.0 39.0 10-11 37.741924215561696 39.0 37.5 39.0 35.0 39.0 12-13 37.6859588115423 39.0 37.0 39.0 35.0 39.0 14-15 39.029638821861816 40.0 38.0 41.0 36.0 41.0 16-17 38.95119819132757 40.0 38.0 41.0 35.0 41.0 18-19 38.95564922113748 40.0 38.0 41.0 35.0 41.0 20-21 38.8979512609651 40.0 38.0 41.0 35.0 41.0 22-23 38.780191600327505 40.0 38.0 41.0 34.5 41.0 24-25 38.9460241553481 40.0 38.0 41.0 35.0 41.0 26-27 38.87517792056828 40.0 38.0 41.0 35.0 41.0 28-29 38.788361299254646 40.0 38.0 41.0 35.0 41.0 30-31 38.68879987868661 40.0 38.0 41.0 34.5 41.0 32-33 38.555941279196574 40.0 38.0 41.0 34.0 41.0 34-35 38.42983122963237 40.0 38.0 41.0 34.0 41.0 36-37 38.29665925361737 40.0 38.0 41.0 34.0 41.0 38-39 38.174132724928285 40.0 38.0 41.0 33.0 41.0 40-41 38.04088271249661 40.0 38.0 41.0 33.0 41.0 42-43 38.09112905647818 40.0 38.0 41.0 33.0 41.0 44-45 37.988494428889126 40.0 38.0 41.0 33.0 41.0 46-47 37.829013841247836 40.0 37.0 41.0 33.0 41.0 48-49 37.6348648985305 40.0 37.0 41.0 33.0 41.0 50-51 37.44819218917012 40.0 37.0 41.0 32.0 41.0 52-53 37.23613721214532 40.0 36.0 41.0 32.0 41.0 54-55 37.03950710525267 39.5 36.0 41.0 31.0 41.0 56-57 36.783242651898995 39.0 35.5 41.0 31.0 41.0 58-59 36.51549502905564 39.0 35.0 41.0 31.0 41.0 60-61 36.2347652974763 38.5 35.0 40.5 30.0 41.0 62-63 35.93185352983084 38.0 35.0 40.0 30.0 41.0 64-65 35.55475817819185 37.0 35.0 40.0 29.0 41.0 66-67 35.19896504510111 37.0 34.0 39.5 29.0 41.0 68-69 34.83741838793995 36.0 34.0 39.0 29.0 41.0 70-71 34.45607534428338 36.0 34.0 39.0 28.5 40.5 72-73 34.03641321981209 35.0 34.0 38.5 28.0 40.0 74-75 33.60132056326046 35.0 33.0 37.0 27.5 39.5 76-77 32.87985912088161 35.0 32.5 36.0 26.0 39.0 78-79 32.931281919233044 35.0 33.0 36.0 26.5 39.0 80-81 32.75863557879635 35.0 33.0 36.0 26.5 37.5 82-83 32.47596749155508 35.0 33.0 35.5 26.0 37.0 84-85 32.183448352939266 35.0 33.0 35.0 26.0 37.0 86-87 31.770633493230033 35.0 32.0 35.0 25.0 36.0 88-89 31.2768887151464 34.5 32.0 35.0 24.0 36.0 90-91 31.053536920579607 34.0 32.0 35.0 23.0 36.0 92-93 30.7513168953027 34.0 31.0 35.0 21.0 35.0 94-95 30.573961537154936 34.0 31.0 35.0 20.0 35.0 96-97 30.307898466990594 34.0 31.0 35.0 18.5 35.0 98-99 29.834748163928666 34.0 31.0 35.0 4.5 35.0 100-101 26.41056411220898 31.0 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18574873715181184 1101 2 0.17127445464253555 1101 3 0.23725210721880785 1101 4 0.18205832986385673 1101 5 0.05209035662284123 1101 6 0.15293778065970542 1101 7 0.13404700714953321 1101 8 0.004795118366303086 1101 9 0.1927262560195544 1101 10-11 0.10806533867152268 1101 12-13 0.28725810901077153 1101 14-15 0.4259856406992881 1101 16-17 0.43039706269381384 1101 18-19 0.34763378920855104 1101 20-21 0.30118469194282227 1101 22-23 0.328445335668448 1101 24-25 0.3287352950189941 1101 26-27 0.3509229245246033 1101 28-29 0.339718924082284 1101 30-31 0.34530097655855485 1101 32-33 0.3386700105020779 1101 34-35 0.3479836854402052 1101 36-37 0.3439527074850588 1101 38-39 0.36700238203309965 1101 40-41 0.4937362401679195 1101 42-43 0.44372010499869674 1101 44-45 0.4945352166295933 1101 46-47 0.5353357303006163 1101 48-49 0.4815847553026771 1101 50-51 0.3577217574617393 1101 52-53 0.38220399065725985 1101 54-55 0.4478975664903615 1101 56-57 0.41183089792628635 1101 58-59 0.11805258680339392 1101 60-61 0.3539149744198866 1101 62-63 0.33227416198073456 1101 64-65 0.307107563016757 1101 66-67 0.225583642628024 1101 68-69 0.2862819932791396 1101 70-71 0.3330247684683698 1101 72-73 0.4453036340311982 1101 74-75 0.3868392520722921 1101 76-77 0.2883046881467948 1101 78-79 0.21420949841930081 1101 80-81 0.3911811742246414 1101 82-83 0.207608640653568 1101 84-85 0.348145772501816 1101 86-87 0.23516408327867921 1101 88-89 0.07275511007669877 1101 90-91 0.3077967921475562 1101 92-93 0.4162378668378999 1101 94-95 0.4446357540486865 1101 96-97 0.21843607654750485 1101 98-99 0.3415646740299181 1101 100-101 0.8295211619880725 1102 1 0.05446363187579806 1102 2 0.09710576615972144 1102 3 0.10928010384233033 1102 4 0.16502198495352616 1102 5 0.14124979430135198 1102 6 0.16214213185580917 1102 7 0.13281102271530187 1102 8 0.1431132239289994 1102 9 0.002808703646991262 1102 10-11 0.08397790994386867 1102 12-13 0.20344923965283357 1102 14-15 0.23752107512873977 1102 16-17 0.3288061101662336 1102 18-19 0.3561897877645279 1102 20-21 0.25758065423882215 1102 22-23 0.3268850090101978 1102 24-25 0.20482246360925416 1102 26-27 0.23335226934999298 1102 28-29 0.22778900323837803 1102 30-31 0.20396109643410654 1102 32-33 0.23189090962186043 1102 34-35 0.3695802322490138 1102 36-37 0.2835804256553942 1102 38-39 0.25698732459051854 1102 40-41 0.22126305688254178 1102 42-43 0.32775755682019536 1102 44-45 0.4395961232880552 1102 46-47 0.5567862816326254 1102 48-49 0.5250559039419969 1102 50-51 0.42521745723133364 1102 52-53 0.3610265172284386 1102 54-55 0.3016457680915394 1102 56-57 0.5544664879072769 1102 58-59 0.472690531293253 1102 60-61 0.5342023001543694 1102 62-63 0.5071535166276391 1102 64-65 0.5769164947545846 1102 66-67 0.5246479111641449 1102 68-69 0.3438621116151097 1102 70-71 0.315482748315695 1102 72-73 0.1698360503359737 1102 74-75 0.1384364094076318 1102 76-77 0.11145240371574516 1102 78-79 0.2490337331375585 1102 80-81 0.2594594097498515 1102 82-83 0.4365089761777199 1102 84-85 0.5313357537613044 1102 86-87 0.42753601870089497 1102 88-89 0.4117179515733902 1102 90-91 0.5620646712845385 1102 92-93 0.4061995164806085 1102 94-95 0.5058307535708799 1102 96-97 0.22588964334972417 1102 98-99 0.40345033169972666 1102 100-101 0.8481057225682314 1103 1 0.0964806571417256 1103 2 0.1339365023796475 1103 3 0.19959898917871755 1103 4 0.04681018934194725 1103 5 0.07116313890632142 1103 6 0.1588261279303751 1103 7 0.20060345334022855 1103 8 0.10464751572731501 1103 9 0.13812378511985912 1103 10-11 0.2281034474667507 1103 12-13 0.21937862315120782 1103 14-15 0.37050464519565907 1103 16-17 0.3369162667951713 1103 18-19 0.30124401318636984 1103 20-21 0.3204288456176556 1103 22-23 0.18644411177467646 1103 24-25 0.2986113560771102 1103 26-27 0.2945850720642085 1103 28-29 0.2501993680458199 1103 30-31 0.30905034622700356 1103 32-33 0.3651097928275817 1103 34-35 0.35319902245748125 1103 36-37 0.28395893200916333 1103 38-39 0.34715941338159695 1103 40-41 0.4324135156648268 1103 42-43 0.5030251119323523 1103 44-45 0.5762485103220811 1103 46-47 0.5221979135590118 1103 48-49 0.4544450423136439 1103 50-51 0.5438245225161538 1103 52-53 0.4907254166773498 1103 54-55 0.46122316950755504 1103 56-57 0.41580986586342306 1103 58-59 0.4315177819960567 1103 60-61 0.3186380750207931 1103 62-63 0.47061355350066947 1103 64-65 0.41356530979156503 1103 66-67 0.47370512327684366 1103 68-69 0.44042760512096635 1103 70-71 0.22372002417287717 1103 72-73 0.16023881493635628 1103 74-75 0.32115016836425525 1103 76-77 0.4250341078215456 1103 78-79 0.37830712627155094 1103 80-81 0.5564252175914532 1103 82-83 0.6026400521760351 1103 84-85 0.5060024613701657 1103 86-87 0.4422188065631296 1103 88-89 0.5048414812579374 1103 90-91 0.6715434041154538 1103 92-93 0.6845995563365754 1103 94-95 0.6577626713071645 1103 96-97 0.6941571495819225 1103 98-99 0.5969888083745829 1103 100-101 0.6089760302487761 1104 1 0.05084203779182417 1104 2 0.12057469984243596 1104 3 0.08926201911307885 1104 4 0.1298078934911544 1104 5 0.13353620484825512 1104 6 0.08437531702409728 1104 7 0.04431267500756775 1104 8 0.1252807388467332 1104 9 0.09984120905753713 1104 10-11 0.18191232769986954 1104 12-13 0.23252580288492908 1104 14-15 0.22176061784167445 1104 16-17 0.12606346615154962 1104 18-19 0.26218266295138193 1104 20-21 0.11141321617189703 1104 22-23 0.13381921356720738 1104 24-25 0.1710683824597723 1104 26-27 0.18890786966631623 1104 28-29 0.24867677255706866 1104 30-31 0.21775287166286716 1104 32-33 0.15407916186106974 1104 34-35 0.15527448628802176 1104 36-37 0.051883002140534984 1104 38-39 0.17930107914889248 1104 40-41 0.19959376448011312 1104 42-43 0.2705450779851546 1104 44-45 0.18154429733777278 1104 46-47 0.2425413345729197 1104 48-49 0.28891321927076064 1104 50-51 0.36856354118712176 1104 52-53 0.22514748039210986 1104 54-55 0.1798465420534896 1104 56-57 0.26264338234155105 1104 58-59 0.1414998272655268 1104 60-61 0.06579340306255688 1104 62-63 0.07310514028677773 1104 64-65 -0.016109542793230958 1104 66-67 -0.09388143716583386 1104 68-69 0.10385612468966343 1104 70-71 0.2541296225952081 1104 72-73 0.24900864608339646 1104 74-75 0.1946183747553576 1104 76-77 0.18900911836871614 1104 78-79 0.11529055984395598 1104 80-81 0.2584355587313354 1104 82-83 0.28000495018727634 1104 84-85 0.3918752525949074 1104 86-87 0.32841537161714385 1104 88-89 0.347717966447739 1104 90-91 0.5491771325182668 1104 92-93 0.5129190965777362 1104 94-95 0.4370537783193207 1104 96-97 0.3450737729152209 1104 98-99 0.27702177443719833 1104 100-101 0.5079248690780886 1105 1 0.030545412033632147 1105 2 0.14605956433507572 1105 3 0.11258668965992058 1105 4 0.05088181174338047 1105 5 -0.02007339433671973 1105 6 0.04783596659159173 1105 7 0.10533225825049897 1105 8 0.12728764392083036 1105 9 0.0908667351921082 1105 10-11 0.14963499747319275 1105 12-13 0.15885988772677706 1105 14-15 0.1708103447516507 1105 16-17 0.2046511332364176 1105 18-19 0.17283529621837346 1105 20-21 0.23142425297065472 1105 22-23 0.08861727316592294 1105 24-25 0.1472596728219031 1105 26-27 0.14823856328667517 1105 28-29 0.07923891822394324 1105 30-31 0.0733081782877818 1105 32-33 0.019695524622719063 1105 34-35 0.05815006760658292 1105 36-37 0.2283809881605947 1105 38-39 0.03089033100486205 1105 40-41 0.2375085877498293 1105 42-43 0.16942689462336347 1105 44-45 0.12752353023309126 1105 46-47 0.1987060234200868 1105 48-49 0.08512981408818376 1105 50-51 -0.020033181732635796 1105 52-53 0.04155929448337048 1105 54-55 0.037493062631931195 1105 56-57 0.1371475517988543 1105 58-59 0.046549162699314195 1105 60-61 0.09738682909242158 1105 62-63 0.18985819898934153 1105 64-65 0.1854912646853819 1105 66-67 0.16709168775751948 1105 68-69 0.1535594517656662 1105 70-71 0.04738479432591447 1105 72-73 0.16250950450108093 1105 74-75 0.0722667348002588 1105 76-77 -0.05844392807073717 1105 78-79 -0.12046006038640655 1105 80-81 -0.0023206034813085807 1105 82-83 0.08756551108304933 1105 84-85 -0.03661961576532491 1105 86-87 0.05806391560868818 1105 88-89 0.13904346711277427 1105 90-91 0.27390864116375724 1105 92-93 0.21876541622983936 1105 94-95 0.18805899957411754 1105 96-97 0.12517027415568194 1105 98-99 0.12513844377659566 1105 100-101 0.38096100280258227 1106 1 -0.06098675918688201 1106 2 -0.14118983887838255 1106 3 -0.10343707129619872 1106 4 -0.07958384526307327 1106 5 -0.1680217679312932 1106 6 -0.11813214690891982 1106 7 -0.020035764815851564 1106 8 0.0029203729690223668 1106 9 -0.1370389133347274 1106 10-11 -0.009072870862780746 1106 12-13 -0.023600479670463415 1106 14-15 0.09173232217017357 1106 16-17 -0.008531196469398594 1106 18-19 0.0566061132718616 1106 20-21 0.0749718825694714 1106 22-23 -0.09283134793861336 1106 24-25 0.022711984587481027 1106 26-27 -0.0546568803876184 1106 28-29 -0.006562044302263814 1106 30-31 -0.038553919762250644 1106 32-33 -0.04223242573547026 1106 34-35 0.058770674868604544 1106 36-37 0.03903164053821229 1106 38-39 0.07209185936476814 1106 40-41 0.0011083861335237088 1106 42-43 -0.008465814601485988 1106 44-45 -0.06317048994883834 1106 46-47 0.14728470170562957 1106 48-49 0.030316647460416846 1106 50-51 0.06779197813398952 1106 52-53 -0.004135610870847017 1106 54-55 0.09389996223986685 1106 56-57 0.10221101357801388 1106 58-59 0.1753888089461242 1106 60-61 0.07158462055935644 1106 62-63 0.0068391018199776 1106 64-65 0.05390702052667251 1106 66-67 0.0475221070134495 1106 68-69 0.0541710945439533 1106 70-71 0.0846877589201469 1106 72-73 -0.10789653586954273 1106 74-75 0.12299274522160886 1106 76-77 -0.03135089026091009 1106 78-79 -0.1839683533795622 1106 80-81 -0.04405845040400891 1106 82-83 0.10243598275020105 1106 84-85 0.10342831321342061 1106 86-87 0.05485164227199135 1106 88-89 3.265560711440685E-5 1106 90-91 0.24717883408621333 1106 92-93 0.21707752798030455 1106 94-95 0.23469376621000393 1106 96-97 0.19741287789279838 1106 98-99 0.23035653444304316 1106 100-101 0.357385256297885 1107 1 0.03886018600261565 1107 2 0.1285312426855043 1107 3 0.10562648117539908 1107 4 0.12292938329672864 1107 5 0.08051955203237782 1107 6 0.13472477365642277 1107 7 0.11418653476894036 1107 8 0.03832781436907595 1107 9 0.14592787087029535 1107 10-11 0.0820299061897174 1107 12-13 0.1384486086380292 1107 14-15 0.23086582622863006 1107 16-17 0.1208225359668802 1107 18-19 0.16696645581222214 1107 20-21 0.17588036528728423 1107 22-23 0.058729397942045125 1107 24-25 0.22054741506182296 1107 26-27 0.2705336029614074 1107 28-29 0.334457436120168 1107 30-31 0.19522741317723558 1107 32-33 0.24298314770138774 1107 34-35 0.15101674761931605 1107 36-37 0.2293554292645439 1107 38-39 0.26782355542481895 1107 40-41 0.21148138800340632 1107 42-43 0.07167609296606514 1107 44-45 0.17162183111285856 1107 46-47 0.13132823221680923 1107 48-49 0.07039924958559851 1107 50-51 0.19516637165100548 1107 52-53 0.22068397170534126 1107 54-55 0.30593713847091664 1107 56-57 0.2648596617989867 1107 58-59 0.2011290060975952 1107 60-61 0.31663011942045927 1107 62-63 0.28617072438558466 1107 64-65 0.38823727606051506 1107 66-67 0.2540546726991124 1107 68-69 0.19734986977140778 1107 70-71 0.4859827907486931 1107 72-73 0.3067387040115577 1107 74-75 0.21627310277733613 1107 76-77 0.04253212171006737 1107 78-79 0.11934390744482926 1107 80-81 0.4015240150179977 1107 82-83 0.47096488953797433 1107 84-85 0.3820526836535336 1107 86-87 0.3847572863109896 1107 88-89 0.5758950064973583 1107 90-91 0.4229285696908427 1107 92-93 0.5891690689987321 1107 94-95 0.7224313594077216 1107 96-97 0.5010315120742206 1107 98-99 0.3666170739942203 1107 100-101 0.6328628163176155 1108 1 -0.08776834108058296 1108 2 -0.03239286729261437 1108 3 0.043413812566939214 1108 4 0.09788568169067702 1108 5 0.05819958496090294 1108 6 0.06867231600612911 1108 7 0.10970350525512629 1108 8 -0.01988811564545756 1108 9 -0.05135490842269519 1108 10-11 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-0.6621608003049602 2316 68-69 -0.7775114540509733 2316 70-71 -0.6629255396243892 2316 72-73 -0.8505049440361248 2316 74-75 -0.7411440179022719 2316 76-77 -0.6406997825071912 2316 78-79 -0.765035141207747 2316 80-81 -0.7620646455668236 2316 82-83 -1.0569880813570478 2316 84-85 -0.8547021094523259 2316 86-87 -0.8960239672808861 2316 88-89 -0.6149744429314339 2316 90-91 -0.8560716562855077 2316 92-93 -0.81645696139487 2316 94-95 -0.8245377815915447 2316 96-97 -0.6710078399071513 2316 98-99 -1.0899032979255487 2316 100-101 -1.0301872528415608 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 15.0 5 35.0 6 60.0 7 128.0 8 304.0 9 584.0 10 980.0 11 1494.0 12 2179.0 13 3255.0 14 4137.0 15 5305.0 16 6514.0 17 8466.0 18 8154.0 19 6109.0 20 6168.0 21 6625.0 22 7037.0 23 8396.0 24 10182.0 25 12478.0 26 15377.0 27 19708.0 28 24480.0 29 30678.0 30 38109.0 31 47704.0 32 59519.0 33 77362.0 34 105703.0 35 155759.0 36 263757.0 37 478104.0 38 521504.0 39 94659.0 40 68.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.74405480068797 18.01109559908139 12.858918638197375 24.385930962033264 2 16.318189227213793 19.017467453196964 39.360296569000404 25.30404675058884 3 20.22265734430996 22.675265606914074 28.11910572728217 28.98297132149379 4 13.055270953762562 15.026859789976186 35.494261000057605 36.42360825620365 5 14.36529806710935 36.93052494137422 33.81453328560885 14.889643705907579 6 35.30815522403344 34.427057613523296 16.2971055628395 13.967681599603763 7 31.253954132311364 30.73069164882332 19.285235657669748 18.730118561195567 8 27.501904137800988 35.58834780904759 18.415314240587506 18.494433812563912 9 26.69076851346288 15.796638575197811 18.90854378138381 38.6040491299555 10-11 26.292386259152995 25.740640220373468 26.4623436344144 21.50462988605914 12-13 26.442479775767158 22.520251311725698 27.370350001944754 23.66691891056239 14-15 23.00338781440429 24.326782048965512 24.772869716602262 27.896960420027934 16-17 23.628257389430864 27.59720318143896 25.485680496737352 23.288858932392824 18-19 23.446546039270288 26.517340578001214 27.00306188312459 23.03305149960391 20-21 23.98013492183061 25.774579513978125 27.116609604047458 23.128675960143806 22-23 23.60837210745007 25.72163429547957 27.006355910819817 23.66363768625054 24-25 23.51451804921376 25.887887002734534 27.19173947765155 23.405855470400155 26-27 23.449295712930336 26.2064260994623 26.95943154833853 23.384846639268837 28-29 23.261442247970702 26.32979306110582 27.129228429412542 23.27953626151093 30-31 23.490763928943416 25.919022118960527 27.098563797535853 23.491650154560208 32-33 23.28646902270791 25.94045571400191 27.162984752174523 23.610090511115658 34-35 23.679533842341915 26.147958775474535 26.933848988650084 23.238658393533466 36-37 23.695910814895267 25.99916843151364 26.862714640307246 23.442206113283852 38-39 23.411834271166228 26.103502484613024 27.023102248484935 23.461560995735812 40-41 23.60039643679536 25.967003972737828 27.06358103740203 23.369018553064777 42-43 23.561089489565486 25.972015600993487 26.853688864905372 23.61320604453565 44-45 23.491421861267778 26.177343078775106 26.834914218612678 23.49632084134444 46-47 23.54881573436878 26.37686683009855 26.5975909677664 23.476726467766273 48-49 23.79186494492854 26.240166514346942 26.538125561350707 23.42984297937381 50-51 23.606185676144428 26.284716879638303 26.591243400669377 23.51785404354789 52-53 23.79921441575403 26.27103518142171 26.44164310447335 23.48810729835091 54-55 23.77700791371352 26.144327602679247 26.502361165371674 23.57630331823556 56-57 23.59946411796101 26.490366530710315 26.509937746301905 23.400231605026775 58-59 23.765378836908567 26.194334684749997 26.493461478716853 23.546824999624583 60-61 23.696852219654353 26.197145166960272 26.764207451988643 23.341795161396732 62-63 23.524385518607378 26.2985924396744 26.680478538016043 23.496543503702174 64-65 23.44561259604797 26.30280961152962 26.59135047018054 23.66022732224187 66-67 23.51158459221418 26.422195230867167 26.409812796298365 23.656407380620283 68-69 23.43303121505901 26.278135574611426 26.672674896349424 23.616158313980137 70-71 23.51467039010427 26.20321619143931 26.60331997766725 23.67879344078917 72-73 23.519004306113146 26.287205831814425 26.485943834419956 23.707846027652472 74-75 23.43251425457005 26.501437888674285 26.31173800639357 23.754309850362095 76-77 23.58738866595977 26.37969609454332 26.28180131330449 23.751113926192417 78-79 23.599571705452625 26.389154142911885 26.399824542164712 23.61144960947078 80-81 23.49799552208126 26.483947559854315 26.402882213997252 23.615174704067176 82-83 23.674796403727434 26.555078693695002 26.307553088149643 23.462571814427925 84-85 23.64074865201723 26.405765769669053 26.10331740510824 23.85016817320548 86-87 23.457703901430285 26.296142934958088 26.517525503853612 23.72862765975801 88-89 23.604905905924742 26.409083661077116 26.255247944647863 23.730762488350283 90-91 23.53069578158478 26.5675191633346 26.310123164380585 23.59166189070004 92-93 23.609128476222125 26.366874166449815 26.330359669033975 23.69363768829408 94-95 23.565929868760875 26.307449274727258 26.387161208893765 23.73945964761811 96-97 23.485612382154667 26.306071927257403 26.340019851781243 23.868295838806688 98-99 23.32824156873944 26.27042808980051 26.321140588092657 24.080189753367396 100-101 23.35659508598347 26.29657109883964 26.23734175968742 24.109492055489472 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 32.0 1 27.5 2 46.5 3 83.0 4 73.5 5 65.5 6 152.0 7 289.5 8 443.0 9 552.0 10 520.5 11 468.5 12 497.0 13 588.0 14 695.0 15 773.5 16 852.5 17 941.5 18 1000.0 19 1035.0 20 1208.0 21 1521.5 22 1923.5 23 2427.0 24 3119.5 25 4142.5 26 5686.5 27 7633.0 28 9563.5 29 12125.5 30 15294.5 31 19016.5 32 23473.5 33 26873.0 34 30289.0 35 34774.0 36 39938.0 37 44777.5 38 48750.5 39 51658.0 40 53799.0 41 57642.0 42 61492.0 43 64529.5 44 68686.5 45 72973.0 46 75496.0 47 77665.0 48 80862.5 49 81512.5 50 80187.5 51 79697.0 52 80278.0 53 81284.5 54 81231.5 55 77141.0 56 70229.5 57 64098.0 58 59379.0 59 53964.0 60 46640.0 61 39244.0 62 32692.5 63 25956.0 64 19534.0 65 14444.0 66 9976.0 67 7802.0 68 5851.5 69 3808.0 70 2825.0 71 1901.0 72 1333.5 73 1132.5 74 915.5 75 695.0 76 443.5 77 210.0 78 111.0 79 54.0 80 30.0 81 19.5 82 9.0 83 3.5 84 4.0 85 2.0 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2675885959500823 2 0.02028454489998784 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.707888275483814E-4 12-13 0.0 14-15 0.0 16-17 4.677261566744769E-4 18-19 0.0 20-21 4.923433228152388E-5 22-23 7.385149842228582E-5 24-25 0.0022155449526685745 26-27 0.002067841955824003 28-29 0.0021909277865278126 30-31 8.616008149266679E-4 32-33 0.0013047098054603829 34-35 0.0015016471345864783 36-37 7.385149842228582E-5 38-39 9.846866456304776E-5 40-41 4.923433228152389E-4 42-43 0.0038648950840996245 44-45 0.0033479345951436244 46-47 0.014154870530938115 48-49 0.014130253364797354 50-51 0.005932737039923627 52-53 0.006425080362738866 54-55 4.1849182439295305E-4 56-57 0.002904825604609909 58-59 0.001206241140897335 60-61 0.0010339209779120014 62-63 0.0 64-65 0.0010585381440527634 66-67 0.0 68-69 0.0 70-71 2.461716614076194E-4 72-73 0.0018216702944163835 74-75 0.0 76-77 0.1933186057034035 78-79 0.10464757326437901 80-81 0.001255475473178859 82-83 0.0 84-85 6.154291535190485E-4 86-87 0.12249501871643143 88-89 0.5780602953173719 90-91 0.30562211763755953 92-93 0.2894978738153604 94-95 0.0019447561251201932 96-97 0.002510950946357718 98-99 0.0022401621188093365 100-101 7.385149842228582E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2031103.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.49716049551081 #Duplication Level Percentage of deduplicated Percentage of total 1 73.91886761182246 40.28368391887905 2 14.286276133637307 15.571229666760361 3 5.016012231421073 8.200752708695987 4 2.2736750180380687 4.956353294906163 5 1.235970461179566 3.3678440295306658 6 0.738400640784795 2.4144442938502197 7 0.5150485536756706 1.9648078584850615 8 0.33403400256815124 1.456312371913153 9 0.25689669575316154 1.260012641330381 >10 1.2948893139317028 12.651628429909406 >50 0.09051535888619754 3.3680234652561203 >100 0.03707014284793064 3.4333309339133296 >500 0.001893097866613627 0.666615623939579 >1k 4.5073758728895287E-4 0.4049607626305469 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2503 0.12323353370065426 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.923433228152388E-5 0.0 0.0 0.0 0.0 7 4.923433228152388E-5 0.0 0.0 0.0 0.0 8 4.923433228152388E-5 0.0 0.0 0.0 0.0 9 4.923433228152388E-5 0.0 0.0 0.0 0.0 10-11 4.923433228152388E-5 0.0 0.0 0.0 0.0 12-13 4.923433228152388E-5 0.0 0.0 2.461716614076194E-5 0.0 14-15 4.923433228152388E-5 0.0 0.0 4.923433228152388E-5 4.923433228152388E-5 16-17 7.385149842228582E-5 0.0 0.0 4.923433228152388E-5 4.923433228152388E-5 18-19 1.9693732912609552E-4 0.0 0.0 4.923433228152388E-5 4.923433228152388E-5 20-21 2.461716614076194E-4 0.0 0.0 9.846866456304776E-5 4.923433228152388E-5 22-23 2.461716614076194E-4 0.0 0.0 1.4770299684457164E-4 4.923433228152388E-5 24-25 2.954059936891433E-4 0.0 0.0 3.4464032597066713E-4 4.923433228152388E-5 26-27 2.954059936891433E-4 0.0 0.0 4.4310899053371495E-4 4.923433228152388E-5 28-29 3.692574921114291E-4 0.0 0.0 5.908119873782867E-4 4.923433228152388E-5 30-31 3.9387465825219104E-4 0.0 0.0 0.0020678419558240032 4.923433228152388E-5 32-33 4.18491824392953E-4 0.0 0.0 0.004898816062011626 4.923433228152388E-5 34-35 4.677261566744769E-4 0.0 0.0 0.008222133491014489 4.923433228152388E-5 36-37 5.415776550967627E-4 0.0 0.0 0.015385728837976212 4.923433228152388E-5 38-39 5.415776550967627E-4 0.0 0.0 0.027866632071342518 4.923433228152388E-5 40-41 5.415776550967627E-4 0.0 0.0 0.04118451895349473 4.923433228152388E-5 42-43 5.661948212375246E-4 0.0 0.0 0.054108531177394746 4.923433228152388E-5 44-45 7.385149842228582E-4 0.0 0.0 0.06939579135080792 4.923433228152388E-5 46-47 9.354523133489537E-4 0.0 0.0 0.09022191390589251 4.923433228152388E-5 48-49 9.354523133489537E-4 0.0 0.0 0.11304202691837884 4.923433228152388E-5 50-51 9.354523133489537E-4 0.0 0.0 0.13903775436302346 4.923433228152388E-5 52-53 9.354523133489537E-4 0.0 0.0 0.16572276245960937 4.923433228152388E-5 54-55 9.354523133489537E-4 0.0 0.0 0.19324475420498122 4.923433228152388E-5 56-57 9.846866456304777E-4 0.0 0.0 0.2268225688209805 4.923433228152388E-5 58-59 9.846866456304777E-4 0.0 0.0 0.2660377144832143 4.923433228152388E-5 60-61 9.846866456304777E-4 0.0 0.0 0.31025014487202274 4.923433228152388E-5 62-63 9.846866456304777E-4 0.0 0.0 0.35539802757418015 4.923433228152388E-5 64-65 0.0010339209779120016 0.0 0.0 0.40391846203762194 4.923433228152388E-5 66-67 0.0010339209779120016 0.0 0.0 0.45972557767872924 4.923433228152388E-5 68-69 0.0010339209779120016 0.0 0.0 0.5231147804911913 4.923433228152388E-5 70-71 0.0011077724763342873 0.0 0.0 0.5946522652962454 4.923433228152388E-5 72-73 0.0011816239747565731 0.0 0.0 0.6712362691601559 4.923433228152388E-5 74-75 0.001255475473178859 0.0 0.0 0.7486572566728522 4.923433228152388E-5 76-77 0.001280092639319621 0.0 0.0 0.8351127441592081 4.923433228152388E-5 78-79 0.0013047098054603829 0.0 0.0 0.9342460722080563 4.923433228152388E-5 80-81 0.0015016471345864783 0.0 0.0 1.0498236672389338 4.923433228152388E-5 82-83 0.0015262643007272403 0.0 0.0 1.1719986627955352 4.923433228152388E-5 84-85 0.00164935013143105 0.0 0.0 1.2905795520955854 4.923433228152388E-5 86-87 0.0017970531282756217 0.0 0.0 1.4182687928677176 4.923433228152388E-5 88-89 0.0018216702944163837 0.0 0.0 1.5652578918942073 4.923433228152388E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3040 0.0 35.53442 1 GTATCAA 4625 0.0 33.954643 1 GTACATG 7190 0.0 29.585276 1 TACATGG 7160 0.0 29.424107 2 ATCAACG 5375 0.0 28.420074 3 TCAACGC 5470 0.0 27.926489 4 GTACCTG 2205 0.0 27.84057 1 CAACGCA 5515 0.0 27.784643 5 ACATGGG 7195 0.0 27.429022 3 TACCTGG 2100 0.0 27.114105 2 TATCAAC 5735 0.0 26.806795 2 AACGCAG 5845 0.0 26.459454 6 ACGCAGA 6830 0.0 22.504642 7 CATGGGG 4270 0.0 22.442501 4 CGCAGAG 6930 0.0 22.111443 8 TGCACCG 660 0.0 21.563791 5 GCACCGT 665 0.0 21.401655 6 GAGTACA 5320 0.0 19.768675 1 TATACTG 990 0.0 19.647009 5 AGTACAT 5035 0.0 18.37674 2 >>END_MODULE