FastQCFastQC Report
Thu 26 May 2016
SRR936324_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936324_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759660
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26980.35515888687044206No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22300.2935523786957323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19370.25498249216754865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14510.1910065029091962No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14250.18758391912171235No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA12430.16362583260932523No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12180.16033488665982149No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10970.14440670826422347No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT10500.13821972987915646No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT9020.1187373298580944No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8960.1179475028302135No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA8420.11083905957928546No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT7840.10320406497643683No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACCG200.002104389570.987499
CGCCGTC250.00160496838.05291494-95
CATTCCG1252.2230415E-730.2879969
AGGACCG650.00595108929.1211555
CATGGGG8250.027.5327284
GTACATG26600.027.4040931
CACCGTG700.0085422227.0428547
ACATGGG26100.026.2916643
GTGTAGC2750.025.8187331
GTACAGG1901.23473E-824.9128111
CATGGGT4400.024.7364354
TACATGG30050.024.7255362
GTACTGC1351.3815841E-524.537276
GATGACC2402.401066E-1023.6624959
TCTCGGG1053.3975084E-822.65054594-95
CGTATAG2303.770765E-922.6381631
ATGGGGG4400.022.585445
GTCTATA1353.8848745E-421.0374851
TAGGACG1353.89179E-421.0319444
CCTGTAC1605.1302093E-520.7046853
Produced by FastQC (version 0.11.5)