##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936324_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 759660 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.920883026617172 33.0 31.0 34.0 30.0 34.0 2 32.03125345549325 34.0 31.0 34.0 30.0 34.0 3 32.09401442750704 34.0 31.0 34.0 30.0 34.0 4 35.60323565805755 37.0 35.0 37.0 33.0 37.0 5 35.61588473790906 37.0 35.0 37.0 35.0 37.0 6 35.714901403259354 37.0 35.0 37.0 35.0 37.0 7 35.67499539267567 37.0 35.0 37.0 35.0 37.0 8 35.68225521944028 37.0 35.0 37.0 35.0 37.0 9 37.381744464628916 39.0 37.0 39.0 35.0 39.0 10-11 37.3237553642419 39.0 37.0 39.0 34.5 39.0 12-13 37.282736355738095 39.0 37.0 39.0 34.0 39.0 14-15 38.541687728720746 40.0 38.0 41.0 34.0 41.0 16-17 38.51957915383198 40.0 38.0 41.0 34.0 41.0 18-19 38.53302530079246 40.0 38.0 41.0 34.0 41.0 20-21 38.48500645025406 40.0 38.0 41.0 34.0 41.0 22-23 38.34654713950978 40.0 38.0 41.0 34.0 41.0 24-25 38.48997709501619 40.0 38.0 41.0 34.0 41.0 26-27 38.44005607771898 40.0 38.0 41.0 34.0 41.0 28-29 38.343360187452284 40.0 38.0 41.0 34.0 41.0 30-31 38.23113695600664 40.0 38.0 41.0 34.0 41.0 32-33 38.05629097227707 40.0 38.0 41.0 34.0 41.0 34-35 37.82255745991628 40.0 38.0 41.0 33.0 41.0 36-37 37.64787865624095 40.0 38.0 41.0 33.0 41.0 38-39 37.51094634441724 40.0 37.5 41.0 33.0 41.0 40-41 37.39413158518285 40.0 37.0 41.0 32.5 41.0 42-43 37.24342337361451 40.0 37.0 41.0 32.0 41.0 44-45 37.08038596214096 40.0 37.0 41.0 31.5 41.0 46-47 36.827009451596766 40.0 37.0 41.0 31.0 41.0 48-49 36.68617868520128 40.0 37.0 41.0 30.5 41.0 50-51 36.38857054471737 39.5 36.5 40.5 30.5 41.0 52-53 36.45240963062423 39.5 37.0 40.5 30.5 41.0 54-55 36.6142142537451 40.0 37.0 41.0 30.5 41.0 56-57 36.53239278098096 40.0 37.0 41.0 30.0 41.0 58-59 36.3934766869389 40.0 37.0 41.0 30.0 41.0 60-61 36.18359858357687 40.0 36.0 41.0 29.0 41.0 62-63 35.94847695021457 39.0 36.0 41.0 29.0 41.0 64-65 35.68674472790459 39.0 35.5 41.0 28.0 41.0 66-67 35.37722599584025 39.0 35.0 40.5 28.0 41.0 68-69 35.012313403364665 38.0 35.0 40.0 28.0 41.0 70-71 34.608738777874315 37.0 35.0 40.0 27.0 41.0 72-73 34.18438972698313 37.0 34.0 39.0 26.0 41.0 74-75 33.74025419266514 36.5 34.0 39.0 26.0 40.5 76-77 33.30902574836111 36.0 34.0 38.5 26.0 40.0 78-79 32.86414053655582 35.5 33.5 37.0 25.5 39.0 80-81 32.4136988916094 35.0 33.0 37.0 24.5 39.0 82-83 32.006727351709976 35.0 33.0 36.5 23.0 39.0 84-85 31.60860187452281 35.0 32.5 36.0 21.5 37.0 86-87 31.270179422373168 35.0 32.0 36.0 20.0 37.0 88-89 30.903392965273937 35.0 32.0 35.0 18.0 36.5 90-91 30.530226680357003 35.0 32.0 35.0 11.0 36.0 92-93 30.227085801542795 34.5 31.0 35.0 4.5 36.0 94-95 29.88501303214596 34.0 31.0 35.0 2.0 36.0 96-97 29.509907063686384 34.0 31.0 35.0 2.0 35.0 98-99 29.11220282758076 34.0 31.0 35.0 2.0 35.0 100-101 26.03628004633652 31.5 24.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1232505675058242 1101 2 0.008950431973801187 1101 3 0.06898874947468414 1101 4 -0.08630284953695622 1101 5 0.037159384580292 1101 6 -0.1750140389558723 1101 7 -0.1420491675187634 1101 8 0.0457978105751593 1101 9 0.009475300632580286 1101 10-11 0.32546870983529885 1101 12-13 -0.012363717323573553 1101 14-15 0.1524953706286425 1101 16-17 0.2243220736454461 1101 18-19 0.2488297102570911 1101 20-21 0.11628438148694187 1101 22-23 0.3733794156129022 1101 24-25 0.26198822804226296 1101 26-27 0.11683806390147566 1101 28-29 0.2219474792260101 1101 30-31 0.49732358221423567 1101 32-33 0.5613260756419152 1101 34-35 0.367981267335054 1101 36-37 0.3379866047870195 1101 38-39 0.4704054015063761 1101 40-41 0.36660391478626053 1101 42-43 0.5790726611295938 1101 44-45 0.5405387442207896 1101 46-47 0.4156600115273079 1101 48-49 0.5178819376116195 1101 50-51 0.5327156082151703 1101 52-53 0.626899513776074 1101 54-55 0.6785495252019658 1101 56-57 0.6835091496894137 1101 58-59 0.7177832043484074 1101 60-61 0.7449221203139373 1101 62-63 0.7582481646812909 1101 64-65 0.6517717931869242 1101 66-67 0.8501039754216322 1101 68-69 0.5794087791935851 1101 70-71 0.6627083835022631 1101 72-73 0.6981241162716358 1101 74-75 0.6187729117577874 1101 76-77 0.5810796209451823 1101 78-79 0.567951707951579 1101 80-81 0.6608247235267797 1101 82-83 0.6698205267407289 1101 84-85 0.6864875788480411 1101 86-87 0.6624694328062333 1101 88-89 0.48669586139515886 1101 90-91 0.6610110500055058 1101 92-93 0.6713384450844515 1101 94-95 0.6069757086308591 1101 96-97 0.7673357819275672 1101 98-99 1.0066816367513098 1101 100-101 1.142709619862405 1102 1 0.3116594672688002 1102 2 0.1961436424638947 1102 3 0.20876243247796822 1102 4 0.22809172701069258 1102 5 0.3556827171490369 1102 6 0.2476329524043237 1102 7 0.2089078373490878 1102 8 0.29996861874092673 1102 9 0.22121422052961748 1102 10-11 0.26624909569557786 1102 12-13 0.327537396421107 1102 14-15 0.34119481248198724 1102 16-17 0.31957018129893555 1102 18-19 0.35326175144270877 1102 20-21 0.3119211529016823 1102 22-23 0.342137220745812 1102 24-25 0.4275347784920953 1102 26-27 0.35940248014124876 1102 28-29 0.435046085133699 1102 30-31 0.5492414148721849 1102 32-33 0.48791959706302634 1102 34-35 0.27899510631357316 1102 36-37 0.19421032219661072 1102 38-39 0.050217823455042776 1102 40-41 0.3271067564917942 1102 42-43 0.288593011818989 1102 44-45 0.16737820697279204 1102 46-47 0.11555042108970781 1102 48-49 0.09235883441819936 1102 50-51 -0.0502392558162299 1102 52-53 0.2976337697079785 1102 54-55 0.28570603563854036 1102 56-57 0.2708106763097007 1102 58-59 0.01056170423371583 1102 60-61 -0.05578384599338193 1102 62-63 0.16692492386267332 1102 64-65 0.027633862152448785 1102 66-67 -0.06641104866277203 1102 68-69 0.014359208635184473 1102 70-71 -0.08453528956142264 1102 72-73 -0.19501373830440372 1102 74-75 0.02434163515662391 1102 76-77 -0.25558092904589813 1102 78-79 -0.40847870212054715 1102 80-81 -0.32367205247546593 1102 82-83 -0.23750164365966242 1102 84-85 -0.28119598513493926 1102 86-87 -0.5528502909239208 1102 88-89 -0.34979648001728236 1102 90-91 -0.2824644960352245 1102 92-93 -0.45677787369629286 1102 94-95 -0.6783213324273234 1102 96-97 -0.36411157643316017 1102 98-99 -0.6040429344442444 1102 100-101 -0.36334461466113055 1103 1 0.29825056750582135 1103 2 0.3613894563640443 1103 3 0.40191557874297246 1103 4 0.2671524350158876 1103 5 0.1634805227916729 1103 6 0.1576079122636429 1103 7 0.0891500194731023 1103 8 0.20311488374589004 1103 9 0.17156879656754143 1103 10-11 0.2902044821930261 1103 12-13 0.30664034771707804 1103 14-15 0.477190492579858 1103 16-17 0.35923061023080294 1103 18-19 0.19694962895627555 1103 20-21 0.22289007254385496 1103 22-23 0.451174131060057 1103 24-25 0.45365489470892584 1103 26-27 0.5025087956087972 1103 28-29 0.2912564223154419 1103 30-31 0.32831951717358265 1103 32-33 0.407616726048424 1103 34-35 0.18520687709115435 1103 36-37 0.24987684868946047 1103 38-39 0.24474483240068423 1103 40-41 0.466705540802522 1103 42-43 0.20143038470683194 1103 44-45 0.48296760600941013 1103 46-47 0.7425909058362521 1103 48-49 0.7622925067173014 1103 50-51 0.691455445613542 1103 52-53 0.8748161804427426 1103 54-55 0.7601044032507502 1103 56-57 0.7250741903398179 1103 58-59 0.8610758872752413 1103 60-61 0.9381639902326313 1103 62-63 0.9188172703723438 1103 64-65 0.8523815492844875 1103 66-67 0.7656527559094428 1103 68-69 0.7628945515513124 1103 70-71 0.8245986274047041 1103 72-73 0.9627582626131002 1103 74-75 0.8973298223268955 1103 76-77 0.7727971006199752 1103 78-79 0.7136326022605175 1103 80-81 0.6052861056405945 1103 82-83 0.8070664617000745 1103 84-85 0.7843026194984439 1103 86-87 0.7361482945948552 1103 88-89 0.7207913898504437 1103 90-91 0.570513082525828 1103 92-93 0.4182388515885158 1103 94-95 0.7531647330211051 1103 96-97 1.0206894404641567 1103 98-99 0.7998726936618787 1103 100-101 1.0928112458786678 1104 1 0.15547369034128522 1104 2 0.09926802095029075 1104 3 0.21723869420978303 1104 4 -0.024931788696434865 1104 5 0.16215877052581362 1104 6 0.13271567882468815 1104 7 0.18763668220982055 1104 8 0.07683458015736022 1104 9 0.09670787990701513 1104 10-11 0.12116295982915659 1104 12-13 0.2624146090095323 1104 14-15 0.2545143817552997 1104 16-17 0.26547600482221156 1104 18-19 0.24190071951702663 1104 20-21 0.18723960463434253 1104 22-23 0.3792031082909162 1104 24-25 0.34195531472219187 1104 26-27 0.2609689311920249 1104 28-29 0.3764841137162236 1104 30-31 0.2601180213368721 1104 32-33 0.2624841823891515 1104 34-35 0.2006465093953338 1104 36-37 0.04192883454164331 1104 38-39 0.22919006488171334 1104 40-41 0.08456255207656227 1104 42-43 0.17040835243212626 1104 44-45 0.21300085705944838 1104 46-47 0.26880028613247475 1104 48-49 0.3846075535082605 1104 50-51 0.3146685470799042 1104 52-53 0.3772040847976186 1104 54-55 0.43138645689454336 1104 56-57 0.4578637784320776 1104 58-59 0.29996211526138694 1104 60-61 0.39568600408569665 1104 62-63 0.6361925559076766 1104 64-65 0.38394701977302503 1104 66-67 0.660847472584706 1104 68-69 0.5036123505344392 1104 70-71 0.4447030780715622 1104 72-73 0.5695853203096561 1104 74-75 0.514634184569914 1104 76-77 0.5341793679547351 1104 78-79 0.6398194160546424 1104 80-81 0.5703794112186742 1104 82-83 0.7385541007834178 1104 84-85 0.5216078107150075 1104 86-87 0.4294418986034003 1104 88-89 0.4416046128995923 1104 90-91 0.4409560307241911 1104 92-93 0.237856510567223 1104 94-95 -0.06013430536958353 1104 96-97 0.22864666542915657 1104 98-99 0.11240953693061684 1104 100-101 0.10944137630383821 1105 1 0.23936237095962198 1105 2 0.32605920890098616 1105 3 0.26215235994290254 1105 4 0.1563415039138789 1105 5 0.16995862498145442 1105 6 0.11311129485221016 1105 7 0.28592543339512844 1105 8 0.32128294498854615 1105 9 0.44135471431722806 1105 10-11 0.38162561447003185 1105 12-13 0.27268761494157445 1105 14-15 0.41881190970643445 1105 16-17 0.28711359937091885 1105 18-19 0.28684525829577723 1105 20-21 0.24736265577333683 1105 22-23 0.35793819550727335 1105 24-25 0.39272824347161617 1105 26-27 0.26234278172237424 1105 28-29 0.37123238813349957 1105 30-31 0.5887009485426375 1105 32-33 0.40852802211393424 1105 34-35 0.4095158943601618 1105 36-37 0.4953303826043651 1105 38-39 0.5685598137074308 1105 40-41 0.4163695073326963 1105 42-43 0.7909532616874202 1105 44-45 0.875711730987156 1105 46-47 0.749687665672468 1105 48-49 0.6372813797812213 1105 50-51 0.6067705604435218 1105 52-53 0.6095711653101077 1105 54-55 0.9270807696385006 1105 56-57 1.0277735850345024 1105 58-59 0.9297468860290223 1105 60-61 0.9514261853545847 1105 62-63 0.8829707330748562 1105 64-65 0.9773192384423979 1105 66-67 0.8858347925838217 1105 68-69 0.8871768641737106 1105 70-71 0.9538602439259805 1105 72-73 1.0413144313864606 1105 74-75 0.9104707338454929 1105 76-77 0.9375213751437457 1105 78-79 1.027971884795683 1105 80-81 1.1031842274137844 1105 82-83 1.1364337841141747 1105 84-85 1.0039583520959177 1105 86-87 1.1542384672848982 1105 88-89 1.2640206492415835 1105 90-91 0.9513558366650479 1105 92-93 0.9892899019712829 1105 94-95 0.8527474729178444 1105 96-97 1.141094015860272 1105 98-99 0.9633963717818723 1105 100-101 0.6961604536408181 1106 1 0.019742098191994017 1106 2 0.022694524153052953 1106 3 0.009161930555855236 1106 4 0.14755140696883728 1106 5 0.011518005111334162 1106 6 -0.03697284807719825 1106 7 0.024347263065060076 1106 8 0.008519030432339036 1106 9 0.06797278801197848 1106 10-11 6.759866605392517E-4 1106 12-13 0.07664284883826866 1106 14-15 0.07832419044441963 1106 16-17 0.2166462189519578 1106 18-19 0.05365371182100631 1106 20-21 0.17960127066715614 1106 22-23 0.02015440670085411 1106 24-25 0.03564423475791756 1106 26-27 0.07585417457178067 1106 28-29 0.15664004255899755 1106 30-31 0.18401991735296974 1106 32-33 0.23482914023409052 1106 34-35 0.1401274018091314 1106 36-37 0.0764348747011212 1106 38-39 0.0484553319349601 1106 40-41 -0.07087380511116237 1106 42-43 -0.10664737428858473 1106 44-45 0.08890410340479349 1106 46-47 0.160526100923704 1106 48-49 0.11981268817795865 1106 50-51 0.014751233380465578 1106 52-53 0.031020901524605904 1106 54-55 0.17920499144545943 1106 56-57 0.09764422173320497 1106 58-59 0.12891578102071577 1106 60-61 0.1863625361325134 1106 62-63 0.12450430249019462 1106 64-65 0.2001452019563743 1106 66-67 0.31411197900945353 1106 68-69 0.45280834911573464 1106 70-71 0.5757694108593583 1106 72-73 0.3052135011683106 1106 74-75 0.23972253285230494 1106 76-77 0.21027661729987557 1106 78-79 0.2980077100674663 1106 80-81 0.28114187719337025 1106 82-83 0.2787509668230612 1106 84-85 0.3349621824059561 1106 86-87 0.13065057147759163 1106 88-89 0.06699507598560928 1106 90-91 0.14156325156712057 1106 92-93 0.18484219964109272 1106 94-95 0.16697846848872544 1106 96-97 0.2292117953128816 1106 98-99 0.1246885593947944 1106 100-101 0.5310400208467563 1107 1 -0.0383803025342182 1107 2 0.13020998785294324 1107 3 0.15923265191370461 1107 4 0.1231134834323413 1107 5 0.05545813473197114 1107 6 0.1116623352705588 1107 7 0.12466603694671363 1107 8 0.25227760671640453 1107 9 0.19798397676412094 1107 10-11 0.13033462416608188 1107 12-13 0.14945523668686178 1107 14-15 0.256354125634104 1107 16-17 -0.10791309198979349 1107 18-19 0.11550851137345575 1107 20-21 0.341942675383315 1107 22-23 0.12131655916343931 1107 24-25 0.136681833187275 1107 26-27 0.07375470023202269 1107 28-29 0.2882440451767465 1107 30-31 0.20366989940875158 1107 32-33 0.1760026925573399 1107 34-35 0.04542939256257483 1107 36-37 0.21896585487802867 1107 38-39 0.08923200022012168 1107 40-41 0.26031220261540966 1107 42-43 -0.1181000120896698 1107 44-45 0.18942237484814228 1107 46-47 0.1239114373251482 1107 48-49 -0.020638872969627187 1107 50-51 0.08596764073548968 1107 52-53 0.10967466242308177 1107 54-55 0.09653277966137352 1107 56-57 0.2102716785087324 1107 58-59 0.39679667358758053 1107 60-61 0.3476948420710002 1107 62-63 0.34111432170107037 1107 64-65 0.12565239020185004 1107 66-67 0.03770863505032196 1107 68-69 0.12855936771440213 1107 70-71 0.17015286839487231 1107 72-73 0.13889586727272274 1107 74-75 0.02249818781651669 1107 76-77 0.14555601944051233 1107 78-79 0.10763742570428292 1107 80-81 0.1307798260628843 1107 82-83 0.30558075001459883 1107 84-85 0.09284603049225382 1107 86-87 -0.20080565516852644 1107 88-89 0.10872595483042247 1107 90-91 0.16908652628265486 1107 92-93 -0.09713018736306722 1107 94-95 0.1027529201343178 1107 96-97 0.2259997790153001 1107 98-99 0.2980297741132816 1107 100-101 0.3689625017942113 1108 1 -0.08659169124285171 1108 2 -0.040320511822592664 1108 3 0.0018956953919655462 1108 4 -0.028314160954415968 1108 5 -0.14630208590397586 1108 6 -0.15903445252956772 1108 7 -0.06713311944523781 1108 8 -0.0491054237832671 1108 9 -0.0898972691800708 1108 10-11 -0.2350308041272342 1108 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11 1360.0 12 2227.0 13 3505.0 14 3800.0 15 3222.0 16 4093.0 17 5739.0 18 4896.0 19 3039.0 20 2702.0 21 2812.0 22 2839.0 23 3312.0 24 3698.0 25 4285.0 26 4842.0 27 6026.0 28 7287.0 29 8888.0 30 11206.0 31 14059.0 32 17777.0 33 23471.0 34 32430.0 35 48740.0 36 75684.0 37 150563.0 38 235990.0 39 61691.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.870101186501614 22.01066521267867 14.112770992939467 23.006462607880245 2 13.991505697975693 22.973058370020855 41.10296905609505 21.93246687590841 3 17.148773930925167 27.873488313309164 31.4337359231788 23.54400183258687 4 10.89341568986762 18.20308468400156 42.062520404831865 28.84097922129896 5 10.73657187993681 40.28014744602422 37.473407056345444 11.509873617693522 6 27.739937020810512 41.176067907995616 18.622040609992418 12.461954461201449 7 25.49314650110846 34.32765675107818 24.367308573324276 15.81188817448908 8 23.08376899247871 37.08195482485647 22.516611219139072 17.317664963525754 9 24.791812863957194 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31.30290421469558 19.10354365669456 34-35 19.35270134536014 30.050776027168542 31.28341787205286 19.313104755418465 36-37 19.57403302348466 29.655798155721442 30.923475137156974 19.84669368363692 38-39 19.197854774020545 30.354616964082016 31.469750924979913 18.97777733691753 40-41 19.10105893947311 30.111912903716544 31.916806116847123 18.870222039963224 42-43 19.014446860631843 30.04218187012905 30.875237430093705 20.06813383914541 44-45 19.17685260089416 30.141016677393345 30.80313823126894 19.878992490443554 46-47 19.568096334917836 29.943455361619765 30.551369758805496 19.937078544656902 48-49 19.402522983574606 30.016743592813818 30.75475829621539 19.825975127396184 50-51 19.433265077259477 29.97006215823721 30.598856076720267 19.997816687783047 52-53 19.13146331879289 30.52156391112734 30.718567706989596 19.628405063090167 54-55 19.63755016294897 29.786423410677042 30.47757574795462 20.098450678419365 56-57 19.77875447699741 30.203089500262912 29.795256945753508 20.222899076986167 58-59 19.60507039086192 29.55126445600447 30.60840060453134 20.23526454860227 60-61 19.658244022974213 30.461865292376235 30.04836119569617 19.831529488953382 62-63 19.609126984126984 30.245171957671957 30.65906084656085 19.48664021164021 64-65 19.519414836266076 29.855495038305786 30.450951261491404 20.17413886393673 66-67 19.60964164245719 30.027662617903395 29.864583450440403 20.498112289199014 68-69 19.3912157098283 29.84712591292177 30.40719899296651 20.354459384283423 70-71 19.872013629305123 29.369472684512804 30.70052564886021 20.057988037321863 72-73 19.450630491793518 29.377643584460767 30.433589189868478 20.738136733877237 74-75 19.442526089625538 30.09336434453123 29.58579411951483 20.8783154463284 76-77 19.272649826476503 29.438011401555265 30.682009843250114 20.607328928718122 78-79 19.04413559281676 29.1829071723232 30.775727904258787 20.997229330601247 80-81 19.48686233980984 29.90313352625052 29.922910293509712 20.68709384042993 82-83 19.833541467131617 29.246657071345794 30.251453849857924 20.668347611664664 84-85 19.167146758667947 29.035964237500355 30.317958703930188 21.478930299901513 86-87 19.356251223680935 29.404788362728134 30.329626572264644 20.909333841326287 88-89 19.75595960665108 28.70950990153046 30.55525970224294 20.979270789575526 90-91 19.572690235821682 28.373969161016337 30.895293954947796 21.15804664821418 92-93 20.078600019848487 29.02160177313176 30.405835456019055 20.493962751000694 94-95 19.500131293749426 28.418052526759784 30.70216690908057 21.379649270410226 96-97 19.523809208921005 28.319901921043535 30.53038153057925 21.625907339456216 98-99 19.526681993431573 28.581639487776606 30.240341923065987 21.65133659572583 100-101 19.545538103040812 28.3627389912279 30.309252036971646 21.782470868759646 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 980.0 1 590.0 2 334.5 3 433.0 4 288.0 5 281.0 6 645.0 7 1048.0 8 1264.0 9 1230.5 10 983.0 11 836.5 12 892.0 13 915.5 14 894.5 15 885.0 16 896.0 17 899.0 18 864.5 19 873.5 20 917.5 21 1039.5 22 1236.5 23 1572.0 24 2002.0 25 2616.5 26 4021.5 27 5935.0 28 7184.5 29 9040.5 30 12906.5 31 16864.0 32 22068.5 33 27346.0 34 30770.5 35 35394.0 36 41506.5 37 44948.5 38 42651.5 39 39302.5 40 36686.0 41 33317.0 42 29498.0 43 28623.5 44 29323.0 45 27763.5 46 24966.0 47 24010.0 48 28983.5 49 27905.0 50 20707.0 51 16513.0 52 13223.5 53 10767.5 54 8628.0 55 7264.0 56 6097.5 57 4994.0 58 4274.0 59 3471.5 60 2665.5 61 2043.5 62 1313.5 63 687.5 64 380.5 65 223.5 66 115.0 67 81.5 68 54.0 69 29.0 70 23.5 71 19.5 72 15.0 73 12.5 74 12.0 75 9.5 76 5.5 77 5.5 78 4.0 79 3.0 80 3.0 81 2.5 82 3.5 83 2.5 84 3.0 85 4.0 86 3.5 87 4.0 88 4.0 89 3.5 90 3.5 91 2.5 92 4.0 93 5.0 94 5.0 95 4.5 96 2.0 97 2.5 98 3.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04857436221467499 2 0.012110681094173709 3 0.010267751362451623 4 0.004738962167285365 5 0.007898270278808942 6 0.005792064871126557 7 0.006318616223047153 8 0.010925940552352367 9 0.016718005423478927 10-11 0.002172024326672459 12-13 0.009675381091540952 14-15 0.008885554063660058 16-17 0.019614037859042203 18-19 0.011189216228312667 20-21 0.0038833162204143963 22-23 0.010136113524471474 24-25 0.022510070294605482 26-27 0.006845167574967749 28-29 0.018758391912171234 30-31 0.014085248663875944 32-33 0.012044862175183635 34-35 0.26577679488192085 36-37 0.4728431140246953 38-39 0.4689597978042808 40-41 0.4890345680962536 42-43 0.48021483295158357 44-45 0.49449753837242977 46-47 0.49982887081062577 48-49 0.5066082194666035 50-51 0.5170076086670352 52-53 0.5050943843298318 54-55 0.49344443566858864 56-57 0.4887054735013032 58-59 0.4873890951215017 60-61 0.4846905194429087 62-63 0.48179448700734534 64-65 0.49614301134718164 66-67 0.47198746807782427 68-69 0.4971302951320328 70-71 0.47942500592370274 72-73 0.4873890951215017 74-75 0.5007503356764869 76-77 0.4884421978253429 78-79 0.48778400863544213 80-81 0.49002185188110464 82-83 0.4917989626938367 84-85 0.4908116789089856 86-87 0.49285206539767795 88-89 0.5058183924387225 90-91 0.5299081167890899 92-93 0.5179948924518863 94-95 0.48989021404312455 96-97 0.4652739383408367 98-99 0.45632256535818655 100-101 0.4624437248242635 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 759660.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.2441264900866 #Duplication Level Percentage of deduplicated Percentage of total 1 67.9728888251156 23.27672202823853 2 13.718372229154681 9.395473477065286 3 5.980032606121146 6.143429789365642 4 3.350042894676491 4.588771705300704 5 2.0461902527741556 3.503499891939023 6 1.3413153802901574 2.755930412745286 7 0.9801111816905745 2.34941358961122 8 0.7168061626482883 1.9637120722081263 9 0.5233888992438351 1.6130696102291773 >10 2.8292511865415295 17.8788165306393 >50 0.29059609114855267 6.9597915100612555 >100 0.23524444999101704 14.97077798944228 >500 0.012300363398223092 2.7118361100329675 >1k 0.0034594772057502444 1.8887552831212042 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2698 0.35515888687044206 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2230 0.2935523786957323 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1937 0.25498249216754865 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1451 0.1910065029091962 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1425 0.18758391912171235 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1243 0.16362583260932523 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1218 0.16033488665982149 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1097 0.14440670826422347 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1050 0.13821972987915646 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 902 0.1187373298580944 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 896 0.1179475028302135 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 842 0.11083905957928546 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 784 0.10320406497643683 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.3163783798014902E-4 0.0 16-17 0.0 0.0 0.0 1.3163783798014902E-4 0.0 18-19 0.0 0.0 0.0 1.9745675697022352E-4 0.0 20-21 0.0 0.0 0.0 3.2909459495037254E-4 0.0 22-23 0.0 0.0 0.0 8.556459468709686E-4 0.0 24-25 0.0 0.0 0.0 0.0029618513545533525 0.0 26-27 0.0 0.0 0.0 0.0071084432509280465 0.0 28-29 0.0 0.0 0.0 0.014940894610746915 0.0 30-31 0.0 0.0 0.0 0.037714240581312695 0.0 32-33 0.0 0.0 0.0 0.08062817576284127 0.0 34-35 0.0 0.0 0.0 0.14473580285917387 0.0 36-37 0.0 0.0 0.0 0.24596530026590843 0.0 38-39 0.0 0.0 0.0 0.3779980517599979 0.0 40-41 0.0 0.0 0.0 0.519377089750678 0.0 42-43 0.0 0.0 0.0 0.6610194034173182 0.0 44-45 0.0 0.0 0.0 0.8206961008872391 0.0 46-47 0.0 0.0 0.0 1.0169681173156413 0.0 48-49 0.0 0.0 0.0 1.2271937445699392 0.0 50-51 0.0 0.0 0.0 1.4221493826185398 0.0 52-53 0.0 0.0 0.0 1.598017534160019 0.0 54-55 0.0 0.0 0.0 1.8035700181660217 0.0 56-57 0.0 0.0 0.0 2.0488771292420296 0.0 58-59 0.0 0.0 0.0 2.295763894373799 0.0 60-61 0.0 0.0 0.0 2.5578548297922756 0.0 62-63 0.0 0.0 0.0 2.7990153489719085 0.0 64-65 0.0 0.0 0.0 3.0640681357449386 0.0 66-67 0.0 0.0 0.0 3.3915830766395496 0.0 68-69 0.0 0.0 0.0 3.742990285127557 0.0 70-71 0.0 0.0 0.0 4.108943474712372 0.0 72-73 0.0 0.0 0.0 4.4522549561646 0.0 74-75 0.0 0.0 0.0 4.83203011873733 0.0 76-77 0.0 0.0 0.0 5.261893478661507 0.0 78-79 0.0 0.0 0.0 5.769686438669931 0.0 80-81 0.0 0.0 0.0 6.283995471658374 0.0 82-83 0.0 0.0 0.0 6.777176631651002 0.0 84-85 0.0 0.0 0.0 7.304649448437459 0.0 86-87 0.0 0.0 0.0 7.885501408524866 0.0 88-89 0.0 0.0 0.0 8.511834241634414 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTACCG 20 0.0021043895 70.98749 9 CGCCGTC 25 0.001604968 38.052914 94-95 CATTCCG 125 2.2230415E-7 30.287996 9 AGGACCG 65 0.005951089 29.121155 5 CATGGGG 825 0.0 27.532728 4 GTACATG 2660 0.0 27.404093 1 CACCGTG 70 0.00854222 27.042854 7 ACATGGG 2610 0.0 26.291664 3 GTGTAGC 275 0.0 25.818733 1 GTACAGG 190 1.23473E-8 24.912811 1 CATGGGT 440 0.0 24.736435 4 TACATGG 3005 0.0 24.725536 2 GTACTGC 135 1.3815841E-5 24.53727 6 GATGACC 240 2.401066E-10 23.662495 9 TCTCGGG 105 3.3975084E-8 22.650545 94-95 CGTATAG 230 3.770765E-9 22.638163 1 ATGGGGG 440 0.0 22.58544 5 GTCTATA 135 3.8848745E-4 21.037485 1 TAGGACG 135 3.89179E-4 21.031944 4 CCTGTAC 160 5.1302093E-5 20.704685 3 >>END_MODULE