FastQCFastQC Report
Thu 26 May 2016
SRR936324_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936324_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759660
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26510.348971908485375No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22690.2986862543769581No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20230.26630334623384144No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14440.19008503804333518No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14000.18429297317220864No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA12270.16151962720164284No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12230.16099307584972222No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11410.15019877313535004No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT9580.12610904878498275No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8620.11347181633888845No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA8420.11083905957928546No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT8310.10939104336150382No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA8270.10886449200958323No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT7870.10359897849037727No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA7860.10346734065239713No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA7840.10320406497643683No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG702.0681942E-433.8851055
CATTCCG1001.311495E-633.2074059
GTATAGT4750.028.0472031
GTACAGG2500.026.6448421
GTAGGGG3350.025.4896936
CCTGTAC1901.20599E-824.9679743
GTCCTAT1551.3890512E-624.5574591
GTGTAGA1903.230998E-722.5379311
AGAGACG1302.9658325E-421.894998
TATAGTA5900.021.7165872
GTAGTAC1100.00289795321.563253
GTACTGG2657.748895E-1021.5456941
GTACATG30700.021.542771
CATGGGG7100.021.3810254
ATCCCGC704.894856E-420.33106438-39
ACATGGG30350.020.31993
GAGTACT25250.020.00898712-13
TACAGGG2851.9845174E-919.9809552
GGGTGCC1908.337047E-619.9743797
TAACCTC1908.337047E-619.9743795
Produced by FastQC (version 0.11.5)