##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936324_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 759660 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48168391122344 34.0 31.0 34.0 31.0 34.0 2 32.62636574256904 34.0 31.0 34.0 31.0 34.0 3 32.71327962509544 34.0 31.0 34.0 31.0 34.0 4 36.19834004686307 37.0 35.0 37.0 35.0 37.0 5 36.16820814574941 37.0 35.0 37.0 35.0 37.0 6 36.20897111865835 37.0 37.0 37.0 35.0 37.0 7 36.161507779796224 37.0 37.0 37.0 35.0 37.0 8 36.139465023826446 37.0 36.0 37.0 35.0 37.0 9 37.917378827370136 39.0 38.0 39.0 35.0 39.0 10-11 37.85953255403733 39.0 38.0 39.0 35.0 39.0 12-13 37.840584603638476 39.0 38.0 39.0 35.0 39.0 14-15 39.17252257588922 41.0 39.0 41.0 36.0 41.0 16-17 39.11920069504778 40.0 38.5 41.0 36.0 41.0 18-19 39.19411118131796 40.5 39.0 41.0 36.0 41.0 20-21 39.16668707053155 40.5 39.0 41.0 36.0 41.0 22-23 39.05856304136061 40.0 39.0 41.0 35.5 41.0 24-25 39.19339309691178 41.0 39.0 41.0 36.0 41.0 26-27 39.102499144354056 41.0 39.0 41.0 35.5 41.0 28-29 39.030719664060236 40.5 39.0 41.0 35.0 41.0 30-31 38.93809072479793 40.0 38.0 41.0 35.0 41.0 32-33 38.78076310454677 40.0 38.0 41.0 35.0 41.0 34-35 38.65602440565516 40.0 38.0 41.0 35.0 41.0 36-37 38.52544691045995 40.0 38.0 41.0 34.5 41.0 38-39 38.41422478477213 40.0 38.0 41.0 34.0 41.0 40-41 38.32633941500145 40.0 38.0 41.0 34.0 41.0 42-43 38.3395137298265 40.0 38.0 41.0 34.0 41.0 44-45 38.21602888134166 40.0 38.0 41.0 34.0 41.0 46-47 38.02403772740436 40.0 38.0 41.0 33.5 41.0 48-49 37.84593107442804 40.0 38.0 41.0 33.0 41.0 50-51 37.70537345654635 40.0 38.0 41.0 33.0 41.0 52-53 37.57526195929758 40.0 38.0 41.0 33.0 41.0 54-55 37.464504515177836 40.0 38.0 41.0 33.0 41.0 56-57 37.288647552852595 40.0 37.5 41.0 32.5 41.0 58-59 37.11759932074875 40.0 37.0 41.0 32.0 41.0 60-61 36.91436234631283 40.0 37.0 41.0 31.0 41.0 62-63 36.68189584814259 39.5 36.0 41.0 31.0 41.0 64-65 36.37668693889371 39.0 36.0 41.0 31.0 41.0 66-67 36.064777005502464 39.0 35.5 40.5 30.0 41.0 68-69 35.71740910407287 38.0 35.0 40.0 30.0 41.0 70-71 35.35687017876418 37.5 35.0 40.0 29.5 41.0 72-73 34.93805057525735 37.0 35.0 39.0 29.0 41.0 74-75 34.490624753179056 36.5 34.0 39.0 29.0 40.5 76-77 33.67046770923834 36.0 33.5 38.0 28.0 39.0 78-79 33.64543611615723 35.5 34.0 37.0 28.0 39.0 80-81 33.41085156517389 35.0 34.0 37.0 28.0 39.0 82-83 33.07679751467762 35.0 34.0 37.0 28.0 39.0 84-85 32.7096714319564 35.0 34.0 36.0 27.5 37.5 86-87 32.30555511676276 35.0 33.0 36.0 26.0 37.0 88-89 31.78500315930811 35.0 33.0 35.0 25.5 36.5 90-91 31.55058842113577 35.0 33.0 35.0 25.0 36.0 92-93 31.28845931074428 35.0 32.5 35.0 24.0 36.0 94-95 31.176632309190953 35.0 32.0 35.0 24.0 36.0 96-97 30.97624595213648 35.0 32.0 35.0 23.5 35.5 98-99 30.658120738224994 34.5 32.0 35.0 19.0 35.0 100-101 27.65840244319827 31.5 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09323269201077977 1101 2 0.12424164970516927 1101 3 0.21744111696972368 1101 4 0.17437306872861313 1101 5 0.23485319639740254 1101 6 0.12857199154006338 1101 7 0.21111502278018435 1101 8 0.06471111482843384 1101 9 0.05830515479090792 1101 10-11 0.0678724310907981 1101 12-13 0.29345426700436406 1101 14-15 0.33004081054460244 1101 16-17 0.3050617917006875 1101 18-19 0.31037950862360475 1101 20-21 0.32227925418121117 1101 22-23 0.3405469277514115 1101 24-25 0.2678685831908325 1101 26-27 0.2934706319156959 1101 28-29 0.483507496966574 1101 30-31 0.43858986234866393 1101 32-33 0.5321522015322486 1101 34-35 0.4817333002829969 1101 36-37 0.3990491736969517 1101 38-39 0.3967338831426872 1101 40-41 0.5621517125444555 1101 42-43 0.6260777930133585 1101 44-45 0.5722623507649374 1101 46-47 0.7813854956218478 1101 48-49 0.6862466107509775 1101 50-51 0.6552073322342906 1101 52-53 0.8106531934963428 1101 54-55 0.7441299686477336 1101 56-57 1.0196411326449848 1101 58-59 0.9197728073217206 1101 60-61 0.8598588553358155 1101 62-63 0.798711448975979 1101 64-65 0.8575076120000915 1101 66-67 0.7246747655510504 1101 68-69 0.7645003739564444 1101 70-71 0.6262367781756311 1101 72-73 0.5477253401841509 1101 74-75 0.46757626894822124 1101 76-77 0.5572672722814431 1101 78-79 0.4106730681325814 1101 80-81 0.6672451333135498 1101 82-83 0.7085992763401308 1101 84-85 0.8241317889474828 1101 86-87 0.560479266255463 1101 88-89 0.5131521507203303 1101 90-91 0.596725310471431 1101 92-93 0.8852643841347714 1101 94-95 0.7305324442026446 1101 96-97 0.7889792272031535 1101 98-99 0.5518050947950641 1101 100-101 0.6753400955940094 1102 1 0.1310464902114603 1102 2 0.13523127684282343 1102 3 0.17960438123559896 1102 4 0.16163764043388795 1102 5 0.08783380143855624 1102 6 -0.003329020260018467 1102 7 0.16714886838509813 1102 8 0.0031544208915903482 1102 9 0.04570735169689044 1102 10-11 0.21661468967326414 1102 12-13 0.18341909611974927 1102 14-15 0.22788085947800596 1102 16-17 0.18104746848536024 1102 18-19 0.34786453551149066 1102 20-21 0.16616895013145694 1102 22-23 0.38233274216127455 1102 24-25 0.35514462366283794 1102 26-27 0.20281130525972912 1102 28-29 0.284139553443886 1102 30-31 0.27279099903088166 1102 32-33 0.322254655848333 1102 34-35 0.3124441089584806 1102 36-37 0.37966950654605114 1102 38-39 0.3683690744162291 1102 40-41 0.4614291802407635 1102 42-43 0.5363550823066205 1102 44-45 0.3099232320759455 1102 46-47 0.5504962839084158 1102 48-49 0.6615110295393691 1102 50-51 0.4929535921420367 1102 52-53 0.363498721951629 1102 54-55 0.5395144271945114 1102 56-57 0.5452266769016845 1102 58-59 0.25475509444866873 1102 60-61 0.33407961635220573 1102 62-63 0.5876759467714265 1102 64-65 0.708871120932983 1102 66-67 0.6388654529124622 1102 68-69 0.48373731867698666 1102 70-71 0.5088934542721688 1102 72-73 0.45998672612089564 1102 74-75 0.41355200613506327 1102 76-77 0.1771617596275732 1102 78-79 0.4054127271279171 1102 80-81 0.4568162014380235 1102 82-83 0.4675882408379266 1102 84-85 0.5970846140870236 1102 86-87 0.4714536017041482 1102 88-89 0.45877872767574956 1102 90-91 0.4565425747882763 1102 92-93 0.6128340250349389 1102 94-95 0.4773466756678424 1102 96-97 0.4010800504057457 1102 98-99 0.4417240488435361 1102 100-101 0.8357358954767733 1103 1 0.028090415588017947 1103 2 0.12109124320110709 1103 3 0.15270534461200214 1103 4 0.003234857346498643 1103 5 0.14420278989333468 1103 6 0.11403947121486624 1103 7 0.22768006343059 1103 8 0.1570891636089229 1103 9 0.2441791385307468 1103 10-11 0.29668340670055215 1103 12-13 0.2894908523702213 1103 14-15 0.3939127617641063 1103 16-17 0.3577548811315836 1103 18-19 0.3432555248837659 1103 20-21 0.34047031109177794 1103 22-23 0.32997782206035 1103 24-25 0.27919988400384455 1103 26-27 0.326346648175857 1103 28-29 0.3590664400560115 1103 30-31 0.37771587860882505 1103 32-33 0.49251805519079284 1103 34-35 0.2896093165431566 1103 36-37 0.474709742802645 1103 38-39 0.39073794818333596 1103 40-41 0.46093220034933324 1103 42-43 0.4729781995174207 1103 44-45 0.5142847084885105 1103 46-47 0.571476959036481 1103 48-49 0.6739498627835019 1103 50-51 0.6316300964619401 1103 52-53 0.6096877463418764 1103 54-55 0.5213149279973308 1103 56-57 0.6103931651653127 1103 58-59 0.6193663032566832 1103 60-61 0.6164645463927272 1103 62-63 0.48768502621175003 1103 64-65 0.5510035469594428 1103 66-67 0.5185772045754433 1103 68-69 0.6335552520052232 1103 70-71 0.4373140139479901 1103 72-73 0.5353269662004081 1103 74-75 0.5974035047205817 1103 76-77 0.6417184917936396 1103 78-79 0.45686209252282595 1103 80-81 0.6553548894111074 1103 82-83 0.5908655365027329 1103 84-85 0.7087354474840666 1103 86-87 0.5864447134099393 1103 88-89 0.6204692238910638 1103 90-91 0.761664334861667 1103 92-93 0.9706810508014314 1103 94-95 0.5587844767229697 1103 96-97 0.6207881702925846 1103 98-99 0.32695753381945636 1103 100-101 0.8624844045370992 1104 1 -0.11424894776030214 1104 2 -0.1125549508892334 1104 3 -0.047275312671914094 1104 4 -0.053190608720854016 1104 5 0.012764428681926177 1104 6 0.03996147401495165 1104 7 0.03816289446736221 1104 8 0.10130384933584224 1104 9 0.023446510131954312 1104 10-11 0.03136812042835402 1104 12-13 0.21858137628150587 1104 14-15 0.3037457696485717 1104 16-17 0.1808864668658714 1104 18-19 0.21262080747163736 1104 20-21 0.0613687341998741 1104 22-23 -0.11659943827418573 1104 24-25 0.10676647821208007 1104 26-27 0.2260523625668327 1104 28-29 0.15503993132415417 1104 30-31 0.08816002421342972 1104 32-33 -0.011193904134238153 1104 34-35 0.019937313743071172 1104 36-37 -0.17527229610384154 1104 38-39 0.06509411206219795 1104 40-41 0.11887929341543213 1104 42-43 0.33454191995069493 1104 44-45 0.16125481017594012 1104 46-47 0.20057346997892722 1104 48-49 0.2721049361016057 1104 50-51 0.2851928405485964 1104 52-53 0.23509993041083987 1104 54-55 0.26622364879904126 1104 56-57 0.29119690107275886 1104 58-59 0.15668853448504194 1104 60-61 0.21221559642589227 1104 62-63 0.29065873853950563 1104 64-65 0.3137328963073145 1104 66-67 0.2468028327531684 1104 68-69 0.31688044911670943 1104 70-71 0.47700803060114794 1104 72-73 0.38531038672947915 1104 74-75 0.39589154907987734 1104 76-77 0.33400811672915864 1104 78-79 0.3082340437423383 1104 80-81 0.293153319887864 1104 82-83 0.3530261178895131 1104 84-85 0.5117229760375963 1104 86-87 0.3247953323768584 1104 88-89 0.3617373692009096 1104 90-91 0.24765007651667048 1104 92-93 0.37642445585633055 1104 94-95 0.4359029646752184 1104 96-97 0.24549802955129962 1104 98-99 0.18283818005038555 1104 100-101 0.3494257684749087 1105 1 0.05494416314009243 1105 2 0.16402563878593668 1105 3 0.19206576832572608 1105 4 0.05791707205185759 1105 5 0.1373040895194677 1105 6 0.09234862200710126 1105 7 0.16506434329529185 1105 8 0.2077055958681342 1105 9 0.1647083356110386 1105 10-11 0.16091141987485003 1105 12-13 0.19014289082491587 1105 14-15 0.24451472900641136 1105 16-17 0.1885286482670665 1105 18-19 0.10556080350225017 1105 20-21 0.19918270204779986 1105 22-23 0.05956657050257519 1105 24-25 0.18178467125682118 1105 26-27 0.1913223914551878 1105 28-29 0.18329979415962327 1105 30-31 0.39440850812636796 1105 32-33 0.3808970831416545 1105 34-35 0.3719297277947149 1105 36-37 0.2982242968350448 1105 38-39 0.387037129240845 1105 40-41 0.5238928555033269 1105 42-43 0.3979904391471152 1105 44-45 0.4475041761758902 1105 46-47 0.6889696152586637 1105 48-49 0.5644318816547838 1105 50-51 0.23627670497182152 1105 52-53 0.44014105771805134 1105 54-55 0.3121318676448297 1105 56-57 0.3285367471485827 1105 58-59 0.3300748665466955 1105 60-61 0.380753757715496 1105 62-63 0.48050481435488734 1105 64-65 0.3796843374169825 1105 66-67 0.3675980341997942 1105 68-69 0.390848958610178 1105 70-71 0.47418645475269017 1105 72-73 0.39225682199532486 1105 74-75 0.44944084671809037 1105 76-77 0.40572730432702286 1105 78-79 0.3461902481218999 1105 80-81 0.536000696781592 1105 82-83 0.5996146463478453 1105 84-85 0.6647584579878938 1105 86-87 0.657090743319138 1105 88-89 0.8855217430293862 1105 90-91 0.6543539378525942 1105 92-93 0.8806474474544501 1105 94-95 0.7165510781116105 1105 96-97 0.8520971430538467 1105 98-99 0.8576623139511987 1105 100-101 1.1214382500062072 1106 1 -0.08427676625211689 1106 2 -0.01289338784039984 1106 3 -0.025217086132812483 1106 4 0.02090657225318182 1106 5 0.021001009785010183 1106 6 0.06971572263791614 1106 7 0.017053316958033804 1106 8 -0.01604232636934455 1106 9 -0.1568993621764676 1106 10-11 -0.036667535100946225 1106 12-13 0.0596356586626996 1106 14-15 0.1762753294668684 1106 16-17 0.12050089658679042 1106 18-19 0.15643578672528236 1106 20-21 0.2212983546975309 1106 22-23 -0.012158307928949341 1106 24-25 -0.013279287693819697 1106 26-27 -0.13419924308574593 1106 28-29 -0.06003846041661376 1106 30-31 -0.1499797263175111 1106 32-33 -0.1385105093381398 1106 34-35 -0.12084946670450591 1106 36-37 -0.16312467434987354 1106 38-39 -0.06208788753612282 1106 40-41 -0.09774065299871637 1106 42-43 0.07449029976925914 1106 44-45 -0.09108903663861412 1106 46-47 -0.1765059729675187 1106 48-49 -0.1519785706521759 1106 50-51 -0.0040169177168323245 1106 52-53 0.032522767213293946 1106 54-55 0.02251913160583996 1106 56-57 -0.06056493674243768 1106 58-59 -0.046458031790059806 1106 60-61 -0.2416809153659898 1106 62-63 -0.14550722376237246 1106 64-65 0.03668678427271743 1106 66-67 0.14478033011447877 1106 68-69 0.3479412267525248 1106 70-71 0.1531672412567744 1106 72-73 0.08675088036883238 1106 74-75 0.0031209383977497396 1106 76-77 0.11521738785049251 1106 78-79 -0.11680131693658069 1106 80-81 0.11418341599199522 1106 82-83 0.21841517082156514 1106 84-85 0.17006617332668839 1106 86-87 0.3777444770971101 1106 88-89 0.4208452545254815 1106 90-91 0.644467401753726 1106 92-93 0.5489952519950734 1106 94-95 0.4425125272283701 1106 96-97 0.43195136413810076 1106 98-99 0.3800556898894172 1106 100-101 0.6765141851283971 1107 1 0.06012991322180028 1107 2 0.02570991811919754 1107 3 0.029466356625107437 1107 4 -0.01043460024359888 1107 5 -0.07623769063087593 1107 6 0.09051835727238 1107 7 -0.04357494202990608 1107 8 0.08320401617535822 1107 9 0.025335854948657754 1107 10-11 -0.04981959657574464 1107 12-13 0.05486671694976053 1107 14-15 0.2907615968569388 1107 16-17 0.144019442471226 1107 18-19 0.1505384699147143 1107 20-21 -0.004483274638111823 1107 22-23 0.09468477510002771 1107 24-25 0.15553998229287913 1107 26-27 0.21328012105536232 1107 28-29 0.19490781246226874 1107 30-31 -0.17691189714653888 1107 32-33 -0.1583283226759704 1107 34-35 -0.03808225608152327 1107 36-37 0.13639294265703228 1107 38-39 0.10664166132495012 1107 40-41 0.12208982685097425 1107 42-43 0.02073256791931044 1107 44-45 0.11879189814996494 1107 46-47 0.16114523351773613 1107 48-49 -0.01443291843236949 1107 50-51 0.0010876865602327257 1107 52-53 0.0037972294143173713 1107 54-55 -0.05684078732847553 1107 56-57 -0.2158035949366166 1107 58-59 -0.13538433380192316 1107 60-61 -0.09163974920247142 1107 62-63 -0.06356861412315595 1107 64-65 -0.21119157499177987 1107 66-67 -0.2958021183222641 1107 68-69 -0.14802237813674424 1107 70-71 -0.012217292932248824 1107 72-73 0.08275142925101164 1107 74-75 0.056952558233504647 1107 76-77 0.05045984599822617 1107 78-79 -0.1798256577502002 1107 80-81 -0.11526305743999643 1107 82-83 -0.14552643655878938 1107 84-85 -0.11711320559201255 1107 86-87 0.1329704687794262 1107 88-89 0.13615906735665817 1107 90-91 0.056718029801604786 1107 92-93 0.32899694086331976 1107 94-95 0.19563376685766087 1107 96-97 -0.107119547217426 1107 98-99 -0.019982309646142227 1107 100-101 0.33859698189421295 1108 1 -0.2246932535530206 1108 2 0.007888698132177296 1108 3 0.040165577909988315 1108 4 0.031701188205460085 1108 5 -0.15447828115650708 1108 6 -0.11176911839277182 1108 7 -0.05194612957046729 1108 8 0.003921083015072213 1108 9 0.06075522548727008 1108 10-11 0.048472908291216754 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-0.9862656175929132 2316 78-79 -1.117523532015234 2316 80-81 -1.027753634855948 2316 82-83 -1.1376190040244936 2316 84-85 -1.1278021849377105 2316 86-87 -1.1771531555232997 2316 88-89 -0.9378949052047787 2316 90-91 -1.0820502500114522 2316 92-93 -1.2082561874345856 2316 94-95 -1.0334857868887575 2316 96-97 -0.9276381662435078 2316 98-99 -1.084312175468547 2316 100-101 -1.1384194510923678 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3.0 5 10.0 6 23.0 7 45.0 8 113.0 9 213.0 10 382.0 11 687.0 12 1182.0 13 1777.0 14 2026.0 15 2745.0 16 3635.0 17 5071.0 18 4385.0 19 2552.0 20 2127.0 21 2094.0 22 2220.0 23 2498.0 24 2969.0 25 3507.0 26 4184.0 27 5159.0 28 6599.0 29 8291.0 30 10738.0 31 13674.0 32 17190.0 33 21777.0 34 30036.0 35 44337.0 36 67839.0 37 144913.0 38 268560.0 39 76066.0 40 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.21638987663903 22.27347963474804 14.444954818680753 23.06517566993218 2 14.0151440342222 23.08062993494464 40.90390213651751 22.000323894315656 3 17.15556959692494 27.849827554432245 31.696943369402103 23.29765947924071 4 10.941868730747967 18.50880657136087 41.85648842903404 28.692836268857118 5 10.622252060132165 40.22904983808546 37.662506910986494 11.486191190795884 6 27.62222573256457 41.32480320143222 18.500250111892164 12.552720954111049 7 25.292367638153912 34.34523339388674 24.339309691177633 16.023089276781718 8 22.975410051865307 37.19835189426849 22.567332754126845 17.258905299739357 9 24.708948740225892 17.301555959244926 23.396519495563805 34.592975804965384 10-11 21.88432162894355 28.29154698561721 30.932932977734716 18.891198407704522 12-13 23.315000546297746 26.57356787716594 30.14628932191944 19.965142254616875 14-15 20.64515704394071 28.75391622567991 28.184516757496773 22.416409972882605 16-17 18.78987410757362 31.845445935103562 29.53045225045794 19.83422770686488 18-19 18.47800331727352 31.322433720348574 31.544967485454016 18.654595476923888 20-21 19.552497169786484 30.1863333596609 30.9184371955875 19.342732274965115 22-23 19.424570761354765 29.94810829595911 30.60998421660245 20.017336726083677 24-25 19.49649933554183 30.057336256642113 31.081832680284055 19.364331727532 26-27 19.18528728006087 30.13524791941467 31.45481687426446 19.22464792626 28-29 18.994934455967417 29.918237801490704 31.724549195542114 19.36227854699977 30-31 19.390931236231353 30.149351380205264 31.220105535235565 19.23961184832782 32-33 18.79336298301122 30.66615678172207 31.398995719730905 19.141484515535804 34-35 19.39146855420632 29.981103102290074 31.272555662256952 19.354872681246654 36-37 19.467064212937366 29.68084406181713 30.910473106389702 19.941618618855802 38-39 19.062475317905378 30.360161124713684 31.4741463286207 19.103217228760236 40-41 18.982379489623245 30.119350309910626 31.885613788900084 19.01265641156605 42-43 19.04967204536404 30.029586675135672 30.86900573632645 20.051735543173837 44-45 19.016331721136453 30.109488090191753 30.840439900687972 20.03374028798382 46-47 19.48780333670374 29.88406323727671 30.662153479946074 19.965979946073475 48-49 19.230460346675304 29.97131226318464 30.806929819710938 19.99129757042912 50-51 19.27707829786786 29.966219596320226 30.60062242302649 20.15607968278542 52-53 19.02670274689361 30.42870392250694 30.786258883746587 19.758334446852864 54-55 19.557786973454448 29.667619509607302 30.54571375351886 20.22887976341939 56-57 19.714877912483487 30.10039630640587 29.860084948189243 20.324640832921407 58-59 19.44782320232529 29.648073844728085 30.659002590673573 20.245100362273053 60-61 19.561665979946003 30.51962156206353 30.002935566294433 19.915776891696034 62-63 19.475949767001026 30.315404259800438 30.61126030066082 19.597385672537715 64-65 19.393039462702756 29.951201468036764 30.304717674098995 20.35104139516148 66-67 19.431127083168786 30.196864386699314 29.718755759155414 20.65325277097649 68-69 19.272503488402705 29.918252902614327 30.2406997867467 20.56854382223626 70-71 19.771252477264454 29.498518084112796 30.500547285392056 20.229682153230698 72-73 19.414596901852505 29.385669010955738 30.20808993645079 20.991644150740967 74-75 19.31976147223758 30.126964694731857 29.39867835610668 21.154595476923888 76-77 19.16572471672257 29.523832762447917 30.325490418489565 20.98495210233995 78-79 18.895963092030748 29.305386167813452 30.47259674208896 21.326053998066836 80-81 19.27874916491422 29.979102286900915 29.619266704183822 21.12288184400104 82-83 19.653331753679275 29.367809283100332 29.881065213384932 21.097793749835454 84-85 19.016778000027646 29.175461771816302 29.882756065084763 21.92500416307129 86-87 19.06102215872246 29.539691159181135 29.994279749257952 21.405006932838457 88-89 19.421362843647334 28.841944407293184 30.093515243567353 21.643177505492133 90-91 19.210973737640515 28.551327974117296 30.4686287779997 21.769069510242485 92-93 19.577939631913505 29.19797703601845 30.035971579217684 21.188111752850364 94-95 19.08243499231874 28.505899497262522 30.26798184932093 22.143683661097807 96-97 18.963982939287032 28.63843399505029 30.069506608393446 22.328076457269233 98-99 19.039150123742825 28.71274551103154 29.769496077089148 22.47860828813649 100-101 18.862259092784946 28.47722221088666 29.978496803481057 22.68202189284734 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 997.0 1 585.0 2 308.5 3 411.0 4 273.0 5 259.0 6 594.0 7 994.5 8 1188.0 9 1048.5 10 717.5 11 543.5 12 515.0 13 517.5 14 519.5 15 501.5 16 510.5 17 562.0 18 617.0 19 684.0 20 806.5 21 978.5 22 1186.5 23 1518.0 24 1890.0 25 2564.5 26 3981.0 27 5694.0 28 6996.5 29 9005.5 30 12773.0 31 16813.0 32 22214.5 33 27461.5 34 31061.0 35 35540.5 36 41244.0 37 44522.5 38 42610.0 39 39552.0 40 36897.5 41 33677.0 42 29570.0 43 28552.5 44 29591.5 45 28457.0 46 25657.0 47 24547.5 48 29273.0 49 27525.5 50 20589.5 51 17395.0 52 14302.0 53 11163.0 54 8666.0 55 7371.0 56 6207.0 57 5058.5 58 4199.0 59 3395.5 60 2631.0 61 2020.5 62 1248.5 63 626.0 64 327.0 65 181.5 66 93.0 67 55.0 68 37.0 69 18.5 70 12.5 71 13.5 72 12.0 73 9.0 74 8.0 75 4.0 76 2.0 77 1.5 78 1.0 79 1.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2693310165073849 2 0.0201405892109628 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.6327567596029803E-4 12-13 1.3163783798014902E-4 14-15 0.0 16-17 4.607324329305215E-4 18-19 0.0 20-21 0.0 22-23 1.3163783798014902E-4 24-25 0.002435300002632757 26-27 0.0023694810836426823 28-29 0.0023694810836426823 30-31 0.0011189216228312665 32-33 0.0015138351367717136 34-35 0.0015138351367717136 36-37 0.0 38-39 0.0 40-41 7.240081088908196E-4 42-43 0.003620040544454098 44-45 0.004475686491325066 46-47 0.014216886501856093 48-49 0.0134270594739752 50-51 0.006318616223047153 52-53 0.007437537845878419 54-55 4.607324329305215E-4 56-57 0.0026985756785930545 58-59 0.0015796540557617882 60-61 0.0014480162177816392 62-63 0.0 64-65 7.898270278808941E-4 66-67 0.0 68-69 0.0 70-71 1.9745675697022352E-4 72-73 0.0016454729747518628 74-75 0.0 76-77 0.1949556380486007 78-79 0.10524445146512915 80-81 9.872837848511177E-4 82-83 0.0 84-85 5.923702709106706E-4 86-87 0.12531922175710186 88-89 0.5909222546928888 90-91 0.31560171655740726 92-93 0.29704078140220624 94-95 0.002501118921622831 96-97 0.0026327567596029803 98-99 0.0026327567596029803 100-101 7.240081088908196E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 759660.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.806321527126414 #Duplication Level Percentage of deduplicated Percentage of total 1 66.26072748838541 21.73770730605275 2 14.372377982950862 9.430097064361572 3 6.284987982493731 6.185620096434446 4 3.5213139364042654 4.620854287825181 5 2.1751473579823077 3.5679291797423573 6 1.4243680386649513 2.8036965509642893 7 0.9647350682344834 2.2154586185896292 8 0.7654674708466995 2.0089737573722473 9 0.5756554996111755 1.6996625468192534 >10 3.0690008999492724 18.293613187344366 >50 0.31993743405056524 7.385406088757598 >100 0.24986352545264282 15.43997942783456 >500 0.013213936442207065 2.853616535451692 >1k 0.0032033785314441367 1.7573853524500638 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2651 0.348971908485375 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2269 0.2986862543769581 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2023 0.26630334623384144 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1444 0.19008503804333518 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1400 0.18429297317220864 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1227 0.16151962720164284 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1223 0.16099307584972222 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1141 0.15019877313535004 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 958 0.12610904878498275 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 862 0.11347181633888845 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 842 0.11083905957928546 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 831 0.10939104336150382 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 827 0.10886449200958323 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 787 0.10359897849037727 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 786 0.10346734065239713 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 784 0.10320406497643683 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.3163783798014902E-4 0.0 16-17 0.0 0.0 0.0 1.3163783798014902E-4 0.0 18-19 0.0 0.0 0.0 1.9745675697022352E-4 0.0 20-21 0.0 0.0 0.0 3.2909459495037254E-4 0.0 22-23 0.0 0.0 0.0 8.556459468709686E-4 0.0 24-25 0.0 0.0 0.0 0.003225127030513651 0.0 26-27 0.0 0.0 0.0 0.007371718926888345 0.0 28-29 0.0 0.0 0.0 0.01520417028670721 0.0 30-31 0.0 0.0 0.0 0.03837242977121344 0.0 32-33 0.0 0.0 0.0 0.08174709738567254 0.0 34-35 0.0 0.0 0.0 0.1458547244820051 0.0 36-37 0.0 0.0 0.0 0.24899297053945185 0.0 38-39 0.0 0.0 0.0 0.3831319274412237 0.0 40-41 0.0 0.0 0.0 0.5262222573256456 0.0 42-43 0.0 0.0 0.0 0.6688518547771372 0.0 44-45 0.0 0.0 0.0 0.8312271279256509 0.0 46-47 0.0 0.0 0.0 1.030000263275676 0.0 48-49 0.0 0.0 0.0 1.2418055445857357 0.0 50-51 0.0 0.0 0.0 1.438999025879999 0.0 52-53 0.0 0.0 0.0 1.6173682963431009 0.0 54-55 0.0 0.0 0.0 1.8248295289998158 0.0 56-57 0.0 0.0 0.0 2.0719795698075454 0.0 58-59 0.0 0.0 0.0 2.320906721428007 0.0 60-61 0.0 0.0 0.0 2.583919121712345 0.0 62-63 0.0 0.0 0.0 2.8265934760287497 0.0 64-65 0.0 0.0 0.0 3.095068846589264 0.0 66-67 0.0 0.0 0.0 3.4235710712687255 0.0 68-69 0.0 0.0 0.0 3.775636468946634 0.0 70-71 0.0 0.0 0.0 4.144156596372062 0.0 72-73 0.0 0.0 0.0 4.490824842692783 0.0 74-75 0.0 0.0 0.0 4.871850564726325 0.0 76-77 0.0 0.0 0.0 5.302437932759393 0.0 78-79 0.0 0.0 0.0 5.8116789089855985 0.0 80-81 0.0 0.0 0.0 6.328818155490614 0.0 82-83 0.0 0.0 0.0 6.822789142511123 0.0 84-85 0.0 0.0 0.0 7.351907432272332 0.0 86-87 0.0 0.0 0.0 7.932430297764789 0.0 88-89 0.0 0.0 0.0 8.559684595740201 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGACCG 70 2.0681942E-4 33.885105 5 CATTCCG 100 1.311495E-6 33.207405 9 GTATAGT 475 0.0 28.047203 1 GTACAGG 250 0.0 26.644842 1 GTAGGGG 335 0.0 25.489693 6 CCTGTAC 190 1.20599E-8 24.967974 3 GTCCTAT 155 1.3890512E-6 24.557459 1 GTGTAGA 190 3.230998E-7 22.537931 1 AGAGACG 130 2.9658325E-4 21.89499 8 TATAGTA 590 0.0 21.716587 2 GTAGTAC 110 0.002897953 21.56325 3 GTACTGG 265 7.748895E-10 21.545694 1 GTACATG 3070 0.0 21.54277 1 CATGGGG 710 0.0 21.381025 4 ATCCCGC 70 4.894856E-4 20.331064 38-39 ACATGGG 3035 0.0 20.3199 3 GAGTACT 2525 0.0 20.008987 12-13 TACAGGG 285 1.9845174E-9 19.980955 2 GGGTGCC 190 8.337047E-6 19.974379 7 TAACCTC 190 8.337047E-6 19.974379 5 >>END_MODULE