##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936323_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 513120 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.61533364515123 33.0 31.0 34.0 30.0 34.0 2 31.66176917680075 33.0 31.0 34.0 30.0 34.0 3 31.760295837231055 34.0 31.0 34.0 30.0 34.0 4 35.20586997193639 37.0 35.0 37.0 33.0 37.0 5 35.27144917368257 37.0 35.0 37.0 33.0 37.0 6 35.449333489242285 37.0 35.0 37.0 33.0 37.0 7 35.422285235422514 37.0 35.0 37.0 33.0 37.0 8 35.443638914873716 37.0 35.0 37.0 33.0 37.0 9 37.025366386030555 39.0 37.0 39.0 33.0 39.0 10-11 36.977307452447775 39.0 37.0 39.0 33.0 39.0 12-13 36.98261128001248 39.0 37.0 39.0 33.0 39.0 14-15 37.9802404895541 40.0 38.0 41.0 33.0 41.0 16-17 38.06653414405987 40.0 38.0 41.0 33.0 41.0 18-19 38.1372105940131 40.0 38.0 41.0 33.5 41.0 20-21 38.04927015123168 40.0 38.0 41.0 33.0 41.0 22-23 37.901585399126915 40.0 38.0 41.0 33.0 41.0 24-25 38.01899458216401 40.0 38.0 41.0 33.5 41.0 26-27 37.87881002494544 40.0 37.5 41.0 33.0 41.0 28-29 37.8477880417836 40.0 38.0 41.0 33.0 41.0 30-31 37.809824212659805 40.0 38.0 41.0 33.0 41.0 32-33 37.539527790770194 40.0 37.0 41.0 33.0 41.0 34-35 37.19514148737137 40.0 37.0 41.0 32.0 41.0 36-37 36.91598846273776 40.0 36.5 41.0 31.0 41.0 38-39 36.74147665263486 40.0 36.0 41.0 31.0 41.0 40-41 36.77424871375117 40.0 36.0 41.0 31.0 41.0 42-43 36.55777595884004 40.0 36.0 41.0 30.0 41.0 44-45 36.32088790146554 40.0 36.0 41.0 30.0 41.0 46-47 35.93525978328656 40.0 35.5 41.0 29.0 41.0 48-49 35.61209950888681 39.0 35.5 41.0 27.5 41.0 50-51 35.19181380573745 38.5 35.0 40.5 27.0 41.0 52-53 35.418826980043654 39.0 35.5 40.5 27.5 41.0 54-55 35.60459444184596 40.0 36.0 41.0 27.5 41.0 56-57 35.36027829747428 39.5 35.5 41.0 26.5 41.0 58-59 35.18549072341753 39.0 35.0 41.0 26.0 41.0 60-61 34.67716518553165 39.0 35.0 41.0 22.5 41.0 62-63 34.584245400686 39.0 34.5 40.0 23.5 41.0 64-65 34.28801157623948 38.0 34.5 40.0 22.5 41.0 66-67 33.77754813688806 37.5 34.0 40.0 22.0 41.0 68-69 33.26024516682258 37.0 33.5 39.0 20.5 41.0 70-71 32.61101301839726 36.0 32.5 39.0 16.0 40.5 72-73 32.09234292173371 36.0 32.0 37.5 13.5 40.0 74-75 31.710378663860304 35.0 32.0 37.0 9.5 39.5 76-77 31.349478679451202 35.0 32.0 37.0 7.0 39.0 78-79 30.84735052229498 35.0 31.0 36.0 2.0 39.0 80-81 30.29195217492984 35.0 31.0 36.0 2.0 37.5 82-83 29.79649107421266 34.0 30.0 35.5 2.0 37.0 84-85 29.450496959775492 34.0 30.0 35.0 2.0 36.5 86-87 29.339751909884626 34.0 30.0 35.0 2.0 36.0 88-89 28.87119874493296 34.0 29.5 35.0 2.0 36.0 90-91 28.404879950109134 34.0 29.0 35.0 2.0 36.0 92-93 27.982838322419706 34.0 28.5 35.0 2.0 35.0 94-95 27.507177658247585 34.0 27.0 35.0 2.0 35.0 96-97 26.997778297474277 34.0 26.0 35.0 2.0 35.0 98-99 26.6057949407546 34.0 25.0 35.0 2.0 35.0 100-101 23.25975990021827 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23421206711050857 1101 2 -0.1022368956333537 1101 3 0.10959708404621438 1101 4 8.645748146207666E-4 1101 5 0.2211242870434731 1101 6 0.20444652380939488 1101 7 0.37350102060808865 1101 8 0.19087076211231135 1101 9 0.16437798486109756 1101 10-11 0.4222666717598287 1101 12-13 0.23492253349778736 1101 14-15 0.37322269492283056 1101 16-17 0.4640423579820805 1101 18-19 0.42338143615757673 1101 20-21 0.49430064181087374 1101 22-23 0.6525175369972445 1101 24-25 0.39302960507355067 1101 26-27 0.34362801422899736 1101 28-29 0.2464016338146422 1101 30-31 0.35478435534331254 1101 32-33 0.3969342012847221 1101 34-35 0.23345957569602405 1101 36-37 0.13780662210325545 1101 38-39 0.2758428731779503 1101 40-41 0.18326622576190488 1101 42-43 -0.021141924385133848 1101 44-45 -0.037139865599016275 1101 46-47 0.04914687656540906 1101 48-49 -0.16631328196078954 1101 50-51 -0.2579961827588022 1101 52-53 -0.07673107627860531 1101 54-55 -0.011433142654446726 1101 56-57 -0.06405558505529996 1101 58-59 -0.11765567657433706 1101 60-61 -0.15975791975944986 1101 62-63 0.09705373942511386 1101 64-65 0.12778802488411145 1101 66-67 0.3815291872740474 1101 68-69 0.3777567145294256 1101 70-71 0.22616818166370223 1101 72-73 -0.028786401209409007 1101 74-75 -0.08031957907613219 1101 76-77 -0.07797511188803696 1101 78-79 -0.05294871996075656 1101 80-81 0.058471548752756064 1101 82-83 0.34374000546319294 1101 84-85 0.46895558806081183 1101 86-87 0.2771479215999335 1101 88-89 -0.1993305027328418 1101 90-91 -0.05770179657815788 1101 92-93 -0.0937156497659295 1101 94-95 0.1701173203438735 1101 96-97 0.0936527422852862 1101 98-99 -0.39072304233994615 1101 100-101 -0.67713368387777 1102 1 0.5126253704614641 1102 2 0.5588193090637468 1102 3 0.4367092789888325 1102 4 0.25946255161709075 1102 5 0.23976932065110645 1102 6 0.38528435552004225 1102 7 0.37522888767261264 1102 8 0.5339075191141589 1102 9 0.6954826556166438 1102 10-11 0.6749702935407598 1102 12-13 0.6390688017338348 1102 14-15 0.7699366839061454 1102 16-17 0.7379552416206536 1102 18-19 0.7327002766484867 1102 20-21 0.8453333793772728 1102 22-23 0.8117570304013242 1102 24-25 0.8478477194926555 1102 26-27 0.9694896500656967 1102 28-29 0.7503536701856319 1102 30-31 0.8808831133622661 1102 32-33 0.9438163815833605 1102 34-35 0.5607157595606935 1102 36-37 0.7237771993456477 1102 38-39 0.689072697879098 1102 40-41 1.0003819132017853 1102 42-43 0.8637037011088609 1102 44-45 0.6239255298803457 1102 46-47 0.8054961753087184 1102 48-49 0.8300532954531761 1102 50-51 0.7496107213568237 1102 52-53 0.897635832003516 1102 54-55 0.9444786748533787 1102 56-57 0.90274118224022 1102 58-59 0.8644590611513792 1102 60-61 0.8919800571655614 1102 62-63 1.2541242082162896 1102 64-65 1.0050451278270032 1102 66-67 1.5256375894871823 1102 68-69 1.5934306745924118 1102 70-71 1.3728009854037353 1102 72-73 1.3907483001207979 1102 74-75 1.4129405139345828 1102 76-77 1.3859418769666085 1102 78-79 1.523898474093425 1102 80-81 1.4464573581849507 1102 82-83 1.251902645546032 1102 84-85 1.153043893096747 1102 86-87 1.5184549733807984 1102 88-89 1.2439807522991124 1102 90-91 1.2945288062758848 1102 92-93 1.3673552601827836 1102 94-95 1.2123857279489414 1102 96-97 1.5420066731828577 1102 98-99 1.4796991821969918 1102 100-101 1.423922030644281 1103 1 0.3242282032407715 1103 2 0.26999722494754863 1103 3 0.2402762684381763 1103 4 0.2596822365426519 1103 5 0.3137222040157539 1103 6 0.2948088532798323 1103 7 0.3283550619752589 1103 8 0.3684327395217295 1103 9 0.2835271707199851 1103 10-11 0.2690937217818359 1103 12-13 0.42539341511727713 1103 14-15 0.4187529868400972 1103 16-17 0.5132898275434741 1103 18-19 0.42237219455569885 1103 20-21 0.5413154577122938 1103 22-23 0.5083808199662911 1103 24-25 0.6340740527778124 1103 26-27 0.6081050569163935 1103 28-29 0.5627167284310417 1103 30-31 0.6076991272371046 1103 32-33 0.40004994134633876 1103 34-35 0.4905726752999655 1103 36-37 0.5483009744576606 1103 38-39 0.43993264873904536 1103 40-41 0.6709470237668924 1103 42-43 0.4048079069189541 1103 44-45 0.6221882846136921 1103 46-47 0.6958213668103852 1103 48-49 0.8209450428155023 1103 50-51 0.4593780287711908 1103 52-53 0.8333143982703177 1103 54-55 0.872624360646121 1103 56-57 1.0875800317849524 1103 58-59 0.7103038364079168 1103 60-61 0.9748995542027146 1103 62-63 0.8857848528181123 1103 64-65 0.8834928803923958 1103 66-67 0.9653138432810877 1103 68-69 0.9745030839001103 1103 70-71 1.2078580745784748 1103 72-73 1.1500429958860892 1103 74-75 0.9668037889743282 1103 76-77 0.8590998477716347 1103 78-79 1.1331859433808908 1103 80-81 1.1952736611189003 1103 82-83 1.2813606597099287 1103 84-85 1.4035338482925859 1103 86-87 1.3626723458334666 1103 88-89 1.2952829636013234 1103 90-91 1.3680191950603913 1103 92-93 1.2724571535199694 1103 94-95 1.3249713617110466 1103 96-97 1.1099355646411588 1103 98-99 1.1905649142391965 1103 100-101 1.5970279708090445 1104 1 0.053090578379503484 1104 2 0.08610710286097856 1104 3 0.22638059696203428 1104 4 0.19258456728627493 1104 5 0.10301743154072085 1104 6 0.1764681207604113 1104 7 0.009575927680032237 1104 8 0.07812197370596863 1104 9 0.06519198759012568 1104 10-11 0.03176297816363416 1104 12-13 0.1565865338742114 1104 14-15 0.19245997907094647 1104 16-17 0.194230449075107 1104 18-19 0.15393712241363033 1104 20-21 0.18573832119731293 1104 22-23 0.3139329842282237 1104 24-25 0.3435218885158804 1104 26-27 0.21929262628376733 1104 28-29 0.3458493588416971 1104 30-31 0.35313752901291195 1104 32-33 0.1278163120455602 1104 34-35 0.24247889061627603 1104 36-37 0.35391140731005066 1104 38-39 0.2295552902484843 1104 40-41 0.2453809860310443 1104 42-43 0.15497438860597157 1104 44-45 0.08107285731068714 1104 46-47 -0.17149550333390096 1104 48-49 -0.010264724110129464 1104 50-51 0.09802120302202866 1104 52-53 -0.1138581877452225 1104 54-55 -0.015053219820025276 1104 56-57 0.1985955700757387 1104 58-59 0.2033254790272352 1104 60-61 0.23502164077319065 1104 62-63 0.3153464510423021 1104 64-65 0.10833194809494984 1104 66-67 0.33416234494590213 1104 68-69 0.32328499289012314 1104 70-71 0.11812167058291578 1104 72-73 0.32548417235667415 1104 74-75 0.2624114471319281 1104 76-77 0.223214720135676 1104 78-79 0.24790292451296736 1104 80-81 0.2817165024063577 1104 82-83 0.47063091498185017 1104 84-85 0.07223806582865677 1104 86-87 0.0652278397291397 1104 88-89 0.2642452277522658 1104 90-91 0.31011686431677177 1104 92-93 0.5387862323213035 1104 94-95 0.23425826515166648 1104 96-97 0.4845887277932128 1104 98-99 0.9477291761703839 1104 100-101 1.0311927876791884 1105 1 0.2508668587029561 1105 2 -0.03291243891799667 1105 3 -0.01751259710804476 1105 4 -0.08554885589431649 1105 5 0.20834930485608538 1105 6 0.11860087008655995 1105 7 0.20052943172316162 1105 8 0.07403673111836895 1105 9 0.2839841034930899 1105 10-11 0.2455433016137647 1105 12-13 0.14839499074752638 1105 14-15 0.09020256667202631 1105 16-17 0.20852879813170944 1105 18-19 0.29464635421955876 1105 20-21 0.25914634006523585 1105 22-23 0.3705578157646059 1105 24-25 0.4799774141223523 1105 26-27 0.30720957372312085 1105 28-29 0.024526077170534677 1105 30-31 0.21703998858163942 1105 32-33 0.408581979161454 1105 34-35 0.07407845261088397 1105 36-37 0.3612185164744659 1105 38-39 0.3363612201676176 1105 40-41 0.18014345233832074 1105 42-43 0.15453217162483668 1105 44-45 0.017183032512846808 1105 46-47 0.07991537941542504 1105 48-49 0.09540407642895588 1105 50-51 -0.124434996438886 1105 52-53 0.16551765457283807 1105 54-55 0.311534549721749 1105 56-57 0.15761679649083504 1105 58-59 0.23385950867682226 1105 60-61 0.15746520546321818 1105 62-63 0.04965827718785221 1105 64-65 -0.03867361120423851 1105 66-67 0.1282366373987358 1105 68-69 0.10101831499254388 1105 70-71 0.25386122583898185 1105 72-73 0.20383238664238945 1105 74-75 0.3668799444365156 1105 76-77 0.23443860827583762 1105 78-79 0.10032880052374793 1105 80-81 0.25444645523654685 1105 82-83 0.35108492441581163 1105 84-85 0.4332134701413253 1105 86-87 0.4222232912116155 1105 88-89 0.6394347493156083 1105 90-91 0.5447235017830785 1105 92-93 0.3444634560409803 1105 94-95 0.3475921600303735 1105 96-97 0.7145836738848566 1105 98-99 1.0156410697013811 1105 100-101 1.0613688321535832 1106 1 0.06315381253003949 1106 2 0.0832267770720847 1106 3 0.22097806759601824 1106 4 0.3056232854133967 1106 5 0.19918347746222054 1106 6 0.10468508142836441 1106 7 0.28897264523416055 1106 8 0.07191111134768846 1106 9 -0.018710930594295405 1106 10-11 0.10340276861097664 1106 12-13 0.030747504692364203 1106 14-15 0.14898394256294978 1106 16-17 0.1881756256805147 1106 18-19 0.004569463918976169 1106 20-21 0.1357393444036319 1106 22-23 -0.047906024489499544 1106 24-25 -0.08720960168179914 1106 26-27 0.18149538484799876 1106 28-29 0.0068892434240268585 1106 30-31 0.12533087407900467 1106 32-33 0.10259683429006827 1106 34-35 0.18667577597624074 1106 36-37 0.3393256012281114 1106 38-39 0.29611486132039033 1106 40-41 0.10986242506840682 1106 42-43 0.44484873886995047 1106 44-45 0.460769374316385 1106 46-47 0.28524589150095636 1106 48-49 0.5228666965095243 1106 50-51 0.38074589912974943 1106 52-53 0.3325385912012919 1106 54-55 0.5385909294862401 1106 56-57 0.47469244725005666 1106 58-59 0.7754180382708782 1106 60-61 0.8154558895351087 1106 62-63 0.8156585289696991 1106 64-65 0.3315546387182877 1106 66-67 0.4488202870733531 1106 68-69 0.6104964487080764 1106 70-71 0.8385228697807179 1106 72-73 0.7966067320096002 1106 74-75 0.6215645392614313 1106 76-77 0.5535696704078497 1106 78-79 0.8879798696281789 1106 80-81 0.7430751157571542 1106 82-83 0.832290862593684 1106 84-85 0.9868284763390314 1106 86-87 0.722093042528634 1106 88-89 0.5550660438616255 1106 90-91 0.7689328099640527 1106 92-93 0.6450231864407314 1106 94-95 0.7038102031044353 1106 96-97 0.8687080541141725 1106 98-99 1.010835425914582 1106 100-101 1.1304514747008412 1107 1 -0.035858942728729915 1107 2 0.06511705065560847 1107 3 0.038359057109200734 1107 4 -0.12532865670353033 1107 5 -0.12523204435026258 1107 6 0.09001582495739768 1107 7 -0.1018845894408642 1107 8 0.17915169377009477 1107 9 0.3325903515478714 1107 10-11 0.21867977842671138 1107 12-13 0.17946748919134592 1107 14-15 0.2667704160339923 1107 16-17 -0.027973789014261286 1107 18-19 0.13753559324850784 1107 20-21 0.47813462982558264 1107 22-23 0.28977515547827437 1107 24-25 0.3891938784858695 1107 26-27 0.37851900027151686 1107 28-29 0.36088666307156814 1107 30-31 0.16055315773819956 1107 32-33 0.5667601809977469 1107 34-35 0.47427637900366193 1107 36-37 0.48799596356441555 1107 38-39 0.4841592282597418 1107 40-41 0.694825019409592 1107 42-43 0.4475094319734225 1107 44-45 0.7196174785134701 1107 46-47 0.6691111271589705 1107 48-49 0.3671759796346805 1107 50-51 0.5265239982701004 1107 52-53 0.7133579712550713 1107 54-55 0.8666548617354479 1107 56-57 0.6019591167522762 1107 58-59 0.9981034006780689 1107 60-61 0.9445727568170881 1107 62-63 1.017462412730957 1107 64-65 0.5037978912856431 1107 66-67 0.6726776777037529 1107 68-69 0.42321768085000144 1107 70-71 0.5349604710926386 1107 72-73 0.43182948826081 1107 74-75 0.28493015063010674 1107 76-77 0.3175312203206566 1107 78-79 0.3385018475203232 1107 80-81 0.45091636264395163 1107 82-83 0.7922103329125427 1107 84-85 0.2912462665932374 1107 86-87 0.29068977502736715 1107 88-89 0.3986380834270342 1107 90-91 0.574380850833812 1107 92-93 0.09838307944589886 1107 94-95 0.559655457571612 1107 96-97 0.590327721503904 1107 98-99 0.38603332382525224 1107 100-101 0.49480574858682047 1108 1 -0.13595406685036338 1108 2 -0.03020871658399571 1108 3 -0.05105761722876778 1108 4 -0.06756595851001634 1108 5 -0.09192106737731365 1108 6 0.1433116346740846 1108 7 0.01585890858432748 1108 8 0.03565016170187363 1108 9 -0.05181192266385182 1108 10-11 -0.18415991770212514 1108 12-13 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2316 72-73 -2.486659194262444 2316 74-75 -2.6504438404626107 2316 76-77 -3.198929857691148 2316 78-79 -3.252177822667612 2316 80-81 -2.875891900683566 2316 82-83 -3.0155899150991665 2316 84-85 -3.2953853427789923 2316 86-87 -3.2797729004533487 2316 88-89 -3.2922206027324883 2316 90-91 -3.538596563939354 2316 92-93 -3.2195458673745527 2316 94-95 -3.1691664246445796 2316 96-97 -3.447713172457103 2316 98-99 -3.4676242273232774 2316 100-101 -3.8768715005344134 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4454.0 3 622.0 4 488.0 5 402.0 6 405.0 7 500.0 8 605.0 9 794.0 10 938.0 11 1449.0 12 2005.0 13 3083.0 14 3085.0 15 3092.0 16 3967.0 17 5407.0 18 4534.0 19 2920.0 20 2874.0 21 2834.0 22 3004.0 23 3237.0 24 3615.0 25 4054.0 26 4653.0 27 5685.0 28 6630.0 29 8095.0 30 10021.0 31 12379.0 32 15541.0 33 20528.0 34 28252.0 35 42153.0 36 62320.0 37 100389.0 38 117108.0 39 20995.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.79598696768634 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31.60697605652282 27.59521482940738 23.60548387286282 44-45 19.93595487462726 27.01431222488261 29.424915904049826 23.624816996440305 46-47 23.03101468322794 30.2161302990803 30.01399475270224 16.738860264989516 48-49 21.004265238064924 29.339940942857563 30.23666929469318 19.419124524384333 50-51 15.135638641732976 30.24776971990738 35.07720275665362 19.53938888170602 52-53 19.332594230690127 32.97511013086426 27.672962750402036 20.01933288804358 54-55 17.55520889335437 28.155825638705522 30.09791738275914 24.191048085180974 56-57 21.742204361092508 27.189653078890576 29.090042028491638 21.978100531525286 58-59 25.048128426029102 26.509802923153153 28.447669761639442 19.994398889178303 60-61 20.0649201227901 29.734689181448314 32.759594812265306 17.440795883496286 62-63 17.789636534544883 29.864973366755443 32.14583659721134 20.199553501488328 64-65 17.145629984066655 34.05700944738618 29.287190942574348 19.51016962597282 66-67 20.14961978247269 28.648732265571685 26.63539552511335 24.56625242684227 68-69 17.717384811095975 28.99443458061534 29.480662052810274 23.807518555478406 70-71 22.935625309653755 28.40922665382032 28.33549723783954 20.319650798686382 72-73 21.047807617962082 24.933070118231367 33.82088839013805 20.198233873668503 74-75 17.49734109752443 33.84396012919426 28.763350824308738 19.895347948972578 76-77 18.779260431426426 27.992483478046292 29.16353723057881 24.06471885994847 78-79 19.09582379834691 28.75311264062575 30.719645450281476 21.431418110745867 80-81 22.36871740335333 27.358954322097468 28.95962961004471 21.31269866450449 82-83 20.340285938112025 26.094007050528788 32.70613004308657 20.85957696827262 84-85 18.519758907543686 28.761096177480084 31.448660135039198 21.270484779937036 86-87 18.748879973285835 30.126784575897908 29.85562794450739 21.26870750630887 88-89 18.053894707976475 29.816123361392272 27.566119688756345 24.563862241874908 90-91 19.79197482852323 25.651278655379205 29.57217501699584 24.98457149910172 92-93 22.640204674925755 28.82590093796281 29.344542654975434 19.189351732136 94-95 20.796914260869904 26.700378177345783 30.531896350813838 21.970811210970474 96-97 16.645928194303984 27.495689954780012 34.0311967974969 21.827185053419107 98-99 19.67674010871917 29.789698012101447 28.095182622945362 22.438379256234025 100-101 17.791253082606513 26.726430970662495 29.97678805862606 25.505527888104933 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1027.0 1 620.0 2 346.0 3 462.0 4 300.5 5 263.5 6 566.5 7 896.0 8 1086.0 9 997.0 10 726.0 11 591.5 12 583.0 13 604.0 14 609.0 15 636.5 16 845.0 17 913.0 18 719.5 19 665.0 20 759.5 21 906.0 22 1041.0 23 1222.5 24 1454.0 25 1883.5 26 2915.0 27 4177.0 28 4743.0 29 5308.5 30 6897.0 31 8652.0 32 11337.0 33 14647.0 34 17197.0 35 19548.5 36 22973.0 37 25622.0 38 24045.5 39 21323.0 40 18829.0 41 15903.5 42 12975.0 43 12869.0 44 13443.5 45 12444.0 46 13971.5 47 29644.0 48 62322.5 49 60332.0 50 26749.5 51 9373.0 52 3958.5 53 2134.0 54 1241.5 55 943.5 56 850.5 57 770.0 58 714.0 59 676.5 60 618.5 61 552.0 62 461.5 63 396.5 64 356.0 65 285.0 66 195.0 67 179.0 68 124.5 69 41.5 70 24.5 71 23.0 72 17.5 73 11.5 74 10.0 75 9.5 76 9.0 77 6.5 78 6.0 79 2.5 80 1.5 81 3.0 82 2.0 83 2.5 84 4.0 85 3.0 86 1.5 87 0.5 88 1.0 89 2.5 90 3.0 91 2.5 92 3.5 93 2.5 94 2.5 95 4.0 96 2.5 97 2.0 98 2.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04696757093857187 2 0.011498285001559088 3 0.010718740255690678 4 0.006821016526348612 5 0.008185219831618335 6 0.006431244153414406 7 0.005067040848144684 8 0.008769878391019644 9 0.013642033052697225 10-11 0.0023386342376052385 12-13 0.008672435297786094 14-15 0.008282662924851886 16-17 0.01773464296850639 18-19 0.011303398815091986 20-21 0.003313065169940754 22-23 0.009257093857187402 24-25 0.02046304957904584 26-27 0.007405675085749922 28-29 0.01822185843467415 30-31 0.014519020891799189 32-33 0.013154817586529468 34-35 0.26562987215466166 36-37 0.4747427502338635 38-39 0.47035781103835367 40-41 0.49043108824446524 42-43 0.47922513252260684 44-45 0.4954981290926099 46-47 0.5011498285001559 48-49 0.5062168693483006 50-51 0.515668849391955 52-53 0.5049501091362645 54-55 0.49432881197380735 56-57 0.48994387277829743 58-59 0.4885796694730277 60-61 0.4857538197692548 62-63 0.48331774243841596 64-65 0.4980316495166823 66-67 0.4726964452759589 68-69 0.49822653570314934 70-71 0.4811739943872778 72-73 0.48935921421889617 74-75 0.5010523854069223 76-77 0.48945665731212973 78-79 0.4873129092609916 80-81 0.4916978484565014 82-83 0.49111318989710007 84-85 0.4925748362956034 86-87 0.4929646086685376 88-89 0.5042680074836295 90-91 0.5263875896476458 92-93 0.5168381665107578 94-95 0.48974898659183036 96-97 0.46665497349547863 98-99 0.45885952603679453 100-101 0.4660703149360773 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 513120.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.831774243841595 #Duplication Level Percentage of deduplicated Percentage of total 1 72.61682012900684 11.496531025880884 2 8.63041016298193 2.732694106641721 3 3.8948249544536906 1.8498596819457438 4 2.3585602442266973 1.4936077330838788 5 1.656900881382638 1.3115840349236048 6 1.2789896105175047 1.2149204864359215 7 0.9626274065685164 1.066806984720923 8 0.747205672362007 0.9463673214842532 9 0.6204145945147472 0.8840037418147801 >10 5.5898862573243395 18.576746180230746 >50 0.9453936678319957 10.523854069223573 >100 0.6376483332512679 17.989359214218897 >500 0.027081589443104046 2.690208917991893 >1k 0.019695701413166577 7.982343311506081 >5k 0.009847850706583288 9.470884003741816 >10k+ 0.0036929440149687334 9.770229186155285 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 26003 5.0676255067040845 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 13708 2.671499844091051 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10422 2.0311038353601494 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7907 1.540965076395385 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7015 1.367126598066729 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6316 1.2309011537262238 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5894 1.1486591830371062 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5610 1.093311506080449 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5478 1.0675865294667914 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5336 1.0399126909884628 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5041 0.9824212659806674 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3913 0.7625896476457749 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3913 0.7625896476457749 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3892 0.7584970377299657 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3481 0.6783988150919863 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3332 0.6493607733083879 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3306 0.6442937324602432 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2527 0.49247739320236983 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2379 0.46363423760523853 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2357 0.4593467415029623 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2207 0.4301138135328968 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2175 0.42387745556594947 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2015 0.39269566573121295 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1754 0.34183037106329905 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1272 0.24789522918615528 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1260 0.24555659494855003 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1176 0.22918615528531336 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 999 0.1946913002806361 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 952 0.18553164951668225 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 876 0.17072029934518243 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 723 0.14090271281571562 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 660 0.12862488306828812 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 658 0.1282351106953539 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 619 0.1206345494231369 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 613 0.11946523230433427 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 595 0.11595728094792643 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 589 0.11478796382912379 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 582 0.11342376052385407 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 568 0.11069535391331463 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 564 0.10991580916744621 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 559 0.10894137823511069 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 558 0.1087464920486436 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 545 0.10621297162457125 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 542 0.10562831306516994 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 539 0.10504365450576864 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 519 0.10114593077642656 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 518 0.10095104458995947 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 517 0.10075615840349236 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.9488618646710322E-4 0.0 14-15 0.0 0.0 0.0 1.9488618646710322E-4 0.0 16-17 0.0 0.0 0.0 5.846585594013096E-4 0.0 18-19 0.0 0.0 0.0 9.744309323355161E-4 0.0 20-21 0.0 0.0 0.0 0.0023386342376052385 0.0 22-23 0.0 0.0 0.0 0.004677268475210477 0.0 24-25 0.0 0.0 0.0 0.008867321484253196 0.0 26-27 0.0 0.0 0.0 0.014226691612098535 0.0 28-29 0.0 0.0 0.0 0.030597131275335206 0.0 30-31 0.0 0.0 0.0 0.07844169005300905 0.0 32-33 0.0 0.0 0.0 0.19702993451824136 0.0 34-35 0.0 0.0 0.0 0.34660508263174306 0.0 36-37 0.0 0.0 0.0 0.5337932647333956 0.0 38-39 0.0 0.0 0.0 0.7978640473963206 0.0 40-41 0.0 0.0 0.0 1.0833723105706268 0.0 42-43 0.0 0.0 0.0 1.3740450576863112 0.0 44-45 0.0 0.0 0.0 1.6781649516682258 0.0 46-47 0.0 0.0 0.0 2.0830410040536327 0.0 48-49 0.0 0.0 0.0 2.4403648269410665 0.0 50-51 0.0 0.0 0.0 2.792913938260056 0.0 52-53 0.0 0.0 0.0 3.1075576863111944 0.0 54-55 0.0 0.0 0.0 3.458547708138447 0.0 56-57 0.0 0.0 0.0 3.9702213907078265 0.0 58-59 0.0 0.0 0.0 4.382308231992516 0.0 60-61 0.0 0.0 0.0 4.818853289678827 0.0 62-63 0.0 0.0 0.0 5.211841284689741 0.0 64-65 0.0 0.0 0.0 5.6035625194886185 0.0 66-67 0.0 0.0 0.0 6.100327408793264 0.0 68-69 0.0 0.0 0.0 6.694437948238229 0.0 70-71 0.0 0.0 0.0 7.283773776114749 0.0 72-73 0.0 0.0 0.0 7.770209697536639 0.0 74-75 0.0 0.0 0.0 8.341323666978484 0.0 76-77 0.0 0.0 0.0 8.854556439039602 0.0 78-79 0.0 0.0 0.0 9.728231212971625 0.0 80-81 0.0 0.0 0.0 10.577057998129092 0.0 82-83 0.0 0.0 0.0 11.21433582787652 0.0 84-85 0.0 0.0 0.0 11.90325849703773 0.0 86-87 0.0 0.0 0.0 12.595884003741816 0.0 88-89 0.0 0.0 0.0 13.42463751169317 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGTCCT 90 2.3283064E-10 47.3372 7 GAACGTA 20 5.4798793E-4 47.3372 20-21 TAGGGTC 55 5.0609517E-5 43.033817 4 CCATGCA 45 9.784002E-4 42.07751 9 GATGACA 85 3.734658E-7 38.983574 9 GGGGTCC 110 1.6716513E-9 38.73044 6 AGATTCC 25 0.0016034802 38.05892 44-45 GCCGAGA 25 0.0016069554 38.042156 94-95 GTAAGCG 75 0.0 38.042156 94-95 AGGAACG 25 0.0016432168 37.86976 18-19 GCTTTGC 115 2.577508E-9 37.053722 1 CTTTGCG 115 2.582965E-9 37.046505 2 ACCATGC 65 1.3542379E-4 36.413227 8 AGGGTCT 65 1.3542379E-4 36.413227 5 AGGGGTC 120 3.914465E-9 35.5029 5 GGATGCC 55 0.0026220744 34.427055 8 AGGATGT 55 0.0026220744 34.427055 6 GTAGGGT 60 0.0040139407 31.561207 3 CTCTGGA 75 3.1386572E-4 31.558134 2 GTCTTCT 75 3.1386572E-4 31.558134 8 >>END_MODULE