##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936323_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 513120 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.286250779544744 33.0 31.0 34.0 30.0 34.0 2 32.37604653882133 34.0 31.0 34.0 30.0 34.0 3 32.50017539756782 34.0 31.0 34.0 30.0 34.0 4 35.95038587464921 37.0 35.0 37.0 35.0 37.0 5 35.958756236357964 37.0 35.0 37.0 35.0 37.0 6 36.06499844091051 37.0 35.0 37.0 35.0 37.0 7 36.035227627065794 37.0 35.0 37.0 35.0 37.0 8 36.03649828500156 37.0 35.0 37.0 35.0 37.0 9 37.7206092142189 39.0 37.0 39.0 35.0 39.0 10-11 37.65085847365138 39.0 37.0 39.0 35.0 39.0 12-13 37.68191845961958 39.0 37.0 39.0 35.0 39.0 14-15 38.76237234954786 40.0 38.0 41.0 34.5 41.0 16-17 38.79470299345182 40.0 38.0 41.0 34.5 41.0 18-19 38.94682238072966 40.0 38.0 41.0 35.0 41.0 20-21 38.8733366463985 40.0 38.0 41.0 35.0 41.0 22-23 38.76767033052697 40.0 38.0 41.0 34.5 41.0 24-25 38.853650218272534 40.0 38.0 41.0 35.0 41.0 26-27 38.686947497661365 40.0 38.0 41.0 34.5 41.0 28-29 38.678149360773304 40.0 38.0 41.0 34.5 41.0 30-31 38.67514031805426 40.0 38.0 41.0 35.0 41.0 32-33 38.437950187090735 40.0 38.0 41.0 34.0 41.0 34-35 38.199648230433425 40.0 38.0 41.0 34.0 41.0 36-37 37.980101145930774 40.0 37.5 41.0 33.5 41.0 38-39 37.83971780480199 40.0 37.0 41.0 33.0 41.0 40-41 37.92871940286872 40.0 38.0 41.0 33.0 41.0 42-43 37.87829747427502 40.0 38.0 41.0 33.0 41.0 44-45 37.69284865138759 40.0 38.0 41.0 33.0 41.0 46-47 37.44336217648893 40.0 38.0 41.0 33.0 41.0 48-49 37.127995400686004 40.0 37.0 41.0 32.0 41.0 50-51 36.855279466791394 40.0 37.0 41.0 31.0 41.0 52-53 36.90143241347053 40.0 37.0 41.0 31.0 41.0 54-55 36.813906103835365 40.0 37.0 41.0 31.0 41.0 56-57 36.51058914094169 40.0 36.5 41.0 30.5 41.0 58-59 36.310264655441216 39.0 36.0 41.0 30.0 41.0 60-61 35.844589959463676 39.0 35.0 41.0 28.0 41.0 62-63 35.72385114593078 39.0 35.0 41.0 28.5 41.0 64-65 35.38599060648581 38.5 35.0 40.0 28.0 41.0 66-67 34.89581579357655 38.0 35.0 40.0 27.5 41.0 68-69 34.417367282507016 37.0 34.0 39.0 26.5 41.0 70-71 33.84856661209854 36.5 34.0 39.0 26.0 40.5 72-73 33.33754287496102 36.0 33.5 38.0 26.0 40.0 74-75 32.94210710944809 35.0 33.0 37.0 26.0 39.5 76-77 32.21079766916121 35.0 32.0 36.5 26.0 39.0 78-79 32.22687967726847 35.0 33.0 36.0 25.0 39.0 80-81 31.944716635484877 35.0 32.5 36.0 25.0 37.5 82-83 31.56405226847521 35.0 32.5 35.5 23.0 37.0 84-85 31.26515824758341 35.0 32.0 35.0 22.5 37.0 86-87 31.108307998129092 35.0 32.0 35.0 21.5 36.0 88-89 30.55165945587777 34.5 31.5 35.0 18.0 36.0 90-91 30.304896515434987 34.0 31.0 35.0 14.5 36.0 92-93 30.049011927034613 34.0 31.0 35.0 10.0 35.0 94-95 29.864261771125662 34.0 31.0 35.0 4.5 35.0 96-97 29.56747349547864 34.0 31.0 35.0 2.0 35.0 98-99 29.302197341752418 34.0 31.0 35.0 2.0 35.0 100-101 26.007097754911133 30.5 24.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14107978662777754 1101 2 0.1884451632299502 1101 3 0.36340706256341804 1101 4 0.15787555118451024 1101 5 0.05844501202564345 1101 6 0.2708344057710548 1101 7 0.16654496348134984 1101 8 0.15482045431205904 1101 9 0.44196238152517964 1101 10-11 0.08079023740751268 1101 12-13 0.4043673394547156 1101 14-15 0.40638073225360216 1101 16-17 0.4424218042676529 1101 18-19 0.5331964729280756 1101 20-21 0.4216943762295102 1101 22-23 0.3654166521689177 1101 24-25 0.48797795885314343 1101 26-27 0.3229303500129461 1101 28-29 0.36180565259847697 1101 30-31 0.33526170875268946 1101 32-33 0.4813241898689711 1101 34-35 0.3435819152038775 1101 36-37 0.1351706289216068 1101 38-39 0.18905695422109403 1101 40-41 0.3463621675031874 1101 42-43 0.059996990284581386 1101 44-45 0.07221608271058244 1101 46-47 0.18979291542112264 1101 48-49 0.07149317177470493 1101 50-51 0.5061319623585518 1101 52-53 0.2861508529839085 1101 54-55 0.1872436696749631 1101 56-57 -0.06421793863660952 1101 58-59 -0.09245379820138311 1101 60-61 0.07700875416939823 1101 62-63 0.008349742384858416 1101 64-65 0.4422058523164196 1101 66-67 0.16898011774933508 1101 68-69 -0.062020788780401404 1101 70-71 -0.10920473507273698 1101 72-73 0.02218361801801194 1101 74-75 0.20878896795350244 1101 76-77 0.07848288472892051 1101 78-79 -0.0339127672050239 1101 80-81 0.030844755316387307 1101 82-83 -0.1266503677145927 1101 84-85 0.18375539810743646 1101 86-87 0.07405082348183711 1101 88-89 -0.21476898830904645 1101 90-91 -0.04278112100417886 1101 92-93 0.38266711531294817 1101 94-95 0.22467525867346083 1101 96-97 0.25472029100672344 1101 98-99 0.4625771954351521 1101 100-101 0.2921484365207334 1102 1 -0.10669144867463132 1102 2 0.1523008802028727 1102 3 0.14352678782029926 1102 4 0.192488037168566 1102 5 0.040400442858874897 1102 6 0.3512782592822461 1102 7 0.3177333316273234 1102 8 0.2987113413450899 1102 9 0.3086163853362933 1102 10-11 0.22880472208034774 1102 12-13 0.41219175875045977 1102 14-15 0.3044610841992821 1102 16-17 0.43153685447995827 1102 18-19 0.5799499822751031 1102 20-21 0.5916901484696737 1102 22-23 0.5500502769521702 1102 24-25 0.45462767363252965 1102 26-27 0.38321575443800526 1102 28-29 0.3270920680095841 1102 30-31 0.333003839911278 1102 32-33 0.26041454751294424 1102 34-35 0.32130039541611666 1102 36-37 0.37668294150624604 1102 38-39 0.2941167862135998 1102 40-41 0.5165233738239934 1102 42-43 0.2854530062642908 1102 44-45 0.4340602438066199 1102 46-47 0.5952442747378157 1102 48-49 0.5198838658013045 1102 50-51 0.42138375915648396 1102 52-53 0.4733303244526539 1102 54-55 0.646203763298395 1102 56-57 0.5602620162672807 1102 58-59 0.32150209829814713 1102 60-61 0.3120538196384075 1102 62-63 0.5055128542611911 1102 64-65 0.5322356917228177 1102 66-67 0.7075323857280367 1102 68-69 0.5695207504918116 1102 70-71 0.7430319949694706 1102 72-73 0.33798196000088865 1102 74-75 0.5020423210572247 1102 76-77 0.39560469935696574 1102 78-79 0.5046548187441005 1102 80-81 0.6250502734620582 1102 82-83 0.7227372198276107 1102 84-85 0.6937825415510375 1102 86-87 0.7412801702623071 1102 88-89 0.9182032494013441 1102 90-91 1.0567121605339942 1102 92-93 0.9722894967059048 1102 94-95 0.7711469173645682 1102 96-97 0.9218332212119229 1102 98-99 1.2276608009177608 1102 100-101 1.404475113685315 1103 1 0.16373491351644276 1103 2 0.19352070965799584 1103 3 0.15643038660625308 1103 4 0.008108791612848165 1103 5 0.09755899178212957 1103 6 0.18839344933861213 1103 7 0.23274417134404501 1103 8 0.28718366393506045 1103 9 0.03469818906515343 1103 10-11 0.198361016342524 1103 12-13 0.27551593854521883 1103 14-15 0.2665567627655534 1103 16-17 0.536020161316209 1103 18-19 0.21934873932091392 1103 20-21 0.39405465201064516 1103 22-23 0.34678969016216854 1103 24-25 0.40257968981984504 1103 26-27 0.3762118521399813 1103 28-29 0.43353808622030954 1103 30-31 0.3760245076378297 1103 32-33 0.41393384220871354 1103 34-35 0.4899952935227816 1103 36-37 0.566187205128152 1103 38-39 0.49149791065354265 1103 40-41 0.4299443884214753 1103 42-43 0.6071555644374413 1103 44-45 0.5147626574502056 1103 46-47 0.5346748282860219 1103 48-49 0.6796815244224916 1103 50-51 0.6556141392692183 1103 52-53 0.6655112754571348 1103 54-55 0.7547425694842644 1103 56-57 0.8867311592070166 1103 58-59 0.8885349065074237 1103 60-61 0.7491343226012575 1103 62-63 0.9892474979958408 1103 64-65 0.794131919501396 1103 66-67 0.8495991583830431 1103 68-69 0.8148018604788021 1103 70-71 0.8859514920066189 1103 72-73 0.7071711491900814 1103 74-75 0.773298282901429 1103 76-77 0.5026577417041267 1103 78-79 0.47617671497188496 1103 80-81 0.557589657766151 1103 82-83 0.7740625558588299 1103 84-85 0.7268711381690451 1103 86-87 0.5275787683256112 1103 88-89 0.6463576069674701 1103 90-91 0.6736307903937977 1103 92-93 0.7906794374487838 1103 94-95 0.9509243418478768 1103 96-97 0.9395669977692265 1103 98-99 1.0956503947897076 1103 100-101 1.4000727434005924 1104 1 0.03518885358302981 1104 2 0.06763280732725008 1104 3 0.09677999814898186 1104 4 0.0033973598703411767 1104 5 0.05061670543363306 1104 6 0.0735210852986512 1104 7 0.1226276341631305 1104 8 0.12768310899610213 1104 9 -0.1072718442311853 1104 10-11 0.07786712067105128 1104 12-13 0.2212873036950569 1104 14-15 0.12294133546254926 1104 16-17 0.15374491159367665 1104 18-19 0.20582210225099118 1104 20-21 0.16494532903617198 1104 22-23 0.14798558361388814 1104 24-25 0.3467861271117414 1104 26-27 0.4166141828836061 1104 28-29 0.33253919609821736 1104 30-31 0.2041155176267324 1104 32-33 0.10359810413989834 1104 34-35 0.30896033236850684 1104 36-37 0.27404514075523423 1104 38-39 0.23188914261803717 1104 40-41 0.3520143107300271 1104 42-43 0.2618420239268957 1104 44-45 0.20536199152346057 1104 46-47 0.3544667261772574 1104 48-49 0.34346065871772424 1104 50-51 0.6382750715666603 1104 52-53 0.7072149380542498 1104 54-55 0.4969234795841544 1104 56-57 0.4575025243568476 1104 58-59 0.5496614325895592 1104 60-61 0.6258518586722914 1104 62-63 0.4358041017694205 1104 64-65 0.33028341783658277 1104 66-67 0.3352095912354329 1104 68-69 0.5291192855620466 1104 70-71 0.604941503105394 1104 72-73 0.41042586617120946 1104 74-75 0.4922577723575827 1104 76-77 0.44565163737560454 1104 78-79 0.2894092343947463 1104 80-81 0.5331473714176482 1104 82-83 0.40484224509301114 1104 84-85 0.4346846786795915 1104 86-87 0.42209874612805365 1104 88-89 0.5469264193981012 1104 90-91 0.6149848414481802 1104 92-93 0.6184985273488977 1104 94-95 0.4792040310820589 1104 96-97 0.5024970754606812 1104 98-99 0.520339628973943 1104 100-101 0.8672231318578625 1105 1 0.05342503956686073 1105 2 0.18383058360757332 1105 3 0.10444509248860356 1105 4 0.07718442186494912 1105 5 0.003320546403983826 1105 6 0.01257937370835549 1105 7 0.04045950747849503 1105 8 0.053811815195558665 1105 9 0.11392087814078167 1105 10-11 0.1538442096198409 1105 12-13 0.1579660435872441 1105 14-15 0.20525686780757013 1105 16-17 0.1530921150977207 1105 18-19 0.06401785696797191 1105 20-21 0.11341652175854477 1105 22-23 0.019195152347045052 1105 24-25 0.10369313519800016 1105 26-27 0.06593611684586875 1105 28-29 0.1008174979850125 1105 30-31 0.19958805805800495 1105 32-33 0.03311714052804149 1105 34-35 0.24212239436311478 1105 36-37 0.3964760706743675 1105 38-39 0.18503520056950862 1105 40-41 0.2010919724550888 1105 42-43 0.0044717409080234916 1105 44-45 0.2363592961056682 1105 46-47 -0.1642327347391941 1105 48-49 0.21947144038888666 1105 50-51 0.16251277372300166 1105 52-53 0.3123994057092361 1105 54-55 0.03543847284560542 1105 56-57 -0.031041950036680532 1105 58-59 -0.0887103666018092 1105 60-61 0.2619704570550425 1105 62-63 0.09009571228155266 1105 64-65 0.1545126968123185 1105 66-67 0.18515062897127876 1105 68-69 0.11162433947040284 1105 70-71 0.14467785755283558 1105 72-73 0.20797472061865108 1105 74-75 0.1777783249182292 1105 76-77 0.11967454842681491 1105 78-79 0.002642051106334975 1105 80-81 0.2827262123879919 1105 82-83 0.23865289199327933 1105 84-85 0.22442103312702955 1105 86-87 0.3069117515188857 1105 88-89 0.2817436363792325 1105 90-91 0.373218500477833 1105 92-93 0.3846710340874431 1105 94-95 0.510559320839473 1105 96-97 0.4212765565927583 1105 98-99 0.14125438638634336 1105 100-101 0.6854508946610984 1106 1 -0.044232420922760696 1106 2 -0.05256835503512747 1106 3 -0.031197532240248904 1106 4 -0.052812479281627134 1106 5 0.10520987860106601 1106 6 0.004904869524906985 1106 7 0.0870149382191201 1106 8 -0.010828330450550538 1106 9 -0.012957801747660369 1106 10-11 0.12399837757769916 1106 12-13 0.012491822174162337 1106 14-15 0.1334675704725825 1106 16-17 -0.11029347910742615 1106 18-19 0.06201812811764995 1106 20-21 0.12317548906563758 1106 22-23 0.14732305752212937 1106 24-25 0.14581817393366237 1106 26-27 -0.02010306428211095 1106 28-29 0.11715426588089173 1106 30-31 0.06587011182310931 1106 32-33 0.07654708707281799 1106 34-35 0.151204940071203 1106 36-37 0.26956349707660365 1106 38-39 0.36930319328720884 1106 40-41 0.17728648361096333 1106 42-43 0.19146333526811077 1106 44-45 0.27873611668205456 1106 46-47 0.4472485171975933 1106 48-49 0.5518622058056764 1106 50-51 0.45640270632294033 1106 52-53 0.5308806770138546 1106 54-55 0.37480892140463595 1106 56-57 0.5119933768923559 1106 58-59 0.6446405591806439 1106 60-61 0.49269946232308826 1106 62-63 0.6232171292322803 1106 64-65 0.9387816579217088 1106 66-67 0.892207299253279 1106 68-69 0.8281615644471572 1106 70-71 0.5688939444756755 1106 72-73 0.6291234268473431 1106 74-75 0.720808809888787 1106 76-77 0.6201006407726481 1106 78-79 0.34237806753026234 1106 80-81 0.3582123450189201 1106 82-83 0.4210882034280523 1106 84-85 0.5195698749305642 1106 86-87 0.6328869219558264 1106 88-89 0.6327299433206619 1106 90-91 0.5763894990833478 1106 92-93 0.7292247991943519 1106 94-95 0.772700531716449 1106 96-97 0.5468019090873355 1106 98-99 0.4558315865722733 1106 100-101 1.0040130266722542 1107 1 0.08992227735086544 1107 2 0.12772550268631733 1107 3 0.1845785347544009 1107 4 -0.005137395750985263 1107 5 0.0032670527843094987 1107 6 0.011922861103315086 1107 7 -0.10834515327469063 1107 8 -0.15647646678390004 1107 9 -0.04887479568429853 1107 10-11 -0.17845815577076252 1107 12-13 0.022269751181390518 1107 14-15 0.05492277583744709 1107 16-17 0.032490749551506326 1107 18-19 -0.005052132138779086 1107 20-21 0.009174477718708829 1107 22-23 -0.24915802225612538 1107 24-25 -0.08038327314311289 1107 26-27 -0.04522605635674637 1107 28-29 -0.001102436655507688 1107 30-31 -0.21931318299397873 1107 32-33 -0.1180923015821449 1107 34-35 -0.14551668904802995 1107 36-37 0.08365997201268272 1107 38-39 0.19088788886705288 1107 40-41 0.0093343666349881 1107 42-43 -0.22282264907018856 1107 44-45 -0.1751719830726728 1107 46-47 0.037681364233733916 1107 48-49 -0.2811899352724936 1107 50-51 -0.4370655992928718 1107 52-53 -0.33383512977162155 1107 54-55 -0.07266483792314204 1107 56-57 -0.041896291773376504 1107 58-59 -0.12083328521370618 1107 60-61 -0.3821510804488497 1107 62-63 -0.18190718610002676 1107 64-65 -0.06242581470339559 1107 66-67 -0.029616527891469957 1107 68-69 0.05859271015200562 1107 70-71 -0.034440664856120407 1107 72-73 -0.07413604035239985 1107 74-75 -0.11345552973474327 1107 76-77 -0.1263182931542559 1107 78-79 -0.13942241356842544 1107 80-81 -0.233565026329714 1107 82-83 -0.14166729163572356 1107 84-85 -0.08105914505535239 1107 86-87 -0.07351055629312597 1107 88-89 0.047011201738712316 1107 90-91 -0.11626027714432752 1107 92-93 -0.048841259718969354 1107 94-95 -0.11591661675779008 1107 96-97 -0.25694716345081403 1107 98-99 -0.2100576662124709 1107 100-101 -0.1509947947257686 1108 1 -0.2180475459462201 1108 2 -0.043690794662040844 1108 3 -0.15140249302447728 1108 4 -0.08277533668635328 1108 5 0.09565580797339379 1108 6 0.006951858617817663 1108 7 0.10324245516461161 1108 8 -0.13287078037787836 1108 9 -0.011456385440702377 1108 10-11 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1917.0 15 2469.0 16 3428.0 17 4732.0 18 4089.0 19 2289.0 20 2048.0 21 2116.0 22 2155.0 23 2384.0 24 2660.0 25 3130.0 26 3666.0 27 4436.0 28 5565.0 29 7143.0 30 9151.0 31 11181.0 32 14061.0 33 18270.0 34 24777.0 35 36793.0 36 58255.0 37 110575.0 38 145960.0 39 25751.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.224230581338546 21.72388861748901 14.534245236932094 18.51763556424035 2 13.909984016217692 27.104791236209113 34.76940470157109 24.215820046002104 3 25.06782039289055 26.37258341128781 31.7263018397256 16.83329435609604 4 10.630651699407547 17.356368880573743 45.10056127221703 26.91241814780168 5 8.666393826005612 43.829708450265045 36.627299657000314 10.87659806672903 6 24.460749922045526 42.32850015590895 16.486786716557532 16.723963205487998 7 23.281493607733083 35.768826005612716 24.181478016838167 16.768202369816027 8 22.78726223885251 28.896554412223264 21.817508574992203 26.498674773932024 9 20.825148113501715 22.064234487059558 27.144917368256937 29.96570003118179 10-11 23.74134579311756 25.80354402256793 28.992579672297282 21.462530512017228 12-13 28.83402404313225 25.340003644375237 27.006964264659594 18.819008047832913 14-15 21.805717960710943 28.76588322419707 31.50081852198316 17.927580293108825 16-17 17.437657919790144 31.22439056237716 31.151892406500277 20.186059111332415 18-19 16.40191378235111 35.43751948861865 30.23318132210789 17.927385406922358 20-21 20.321971782401565 28.496188509694136 28.077962346003222 23.10387736190108 22-23 18.103952291861553 29.355901153726222 30.029622700343 22.510523854069223 24-25 23.421837072175357 29.383585604881656 28.335650924909057 18.85892639803393 26-27 20.78284400710578 25.80804847365103 35.47549373566932 17.93361378357388 28-29 16.93141233963974 34.2757051466325 31.00978849255266 17.783094021175106 30-31 19.161058718289027 28.166615020921398 29.86868338429696 22.80364287649261 32-33 19.27340903532099 30.10679027924893 31.89052607781785 18.729274607612233 34-35 22.585303678757686 27.63979513084965 30.477891593972416 19.29700959642025 36-37 20.59678164324455 26.077868876420478 34.248780497311536 19.07656898302343 38-39 18.50855405027484 30.6986968922444 32.62972855251067 18.16302050497009 40-41 17.98616883300381 31.459619793945432 31.484565395967586 19.069645977083177 42-43 17.18462836751439 31.494286699889496 27.692208746426143 23.628876186169975 44-45 19.89789256013113 27.04541335095218 29.320271221059972 23.736422867856717 46-47 22.957213612273577 30.082040866448228 30.0989012488378 16.86184427244039 48-49 21.026230281482867 29.166739761379873 30.364518461775365 19.4425114953619 50-51 15.128336810675066 30.11936653585625 35.08259376885049 19.669702884618196 52-53 19.22365898842953 32.90054933649857 27.737254019160996 20.1385376559109 54-55 17.34141333824448 28.176629764859385 30.077750071377274 24.404206825518862 56-57 21.667490092096966 27.115350696403528 29.093325771461924 22.123833440037576 58-59 24.922896724974443 26.57770597171564 28.46112094763214 20.038276355677773 60-61 19.91910124622279 29.902428424286775 32.72803811264825 17.45043221684218 62-63 17.642169473027753 30.091011849080136 31.91670564390396 20.35011303398815 64-65 16.815187194331113 34.349996540746595 29.025864496659143 19.808951768263153 66-67 19.943093233551608 28.992633302151543 26.17613813532897 24.888135328967884 68-69 17.55515279077019 29.393903960087307 29.15682491425008 23.894118334892422 70-71 22.823276975111888 28.710926271129463 27.918416188136614 20.547380565622035 72-73 20.983506235590124 25.348419323215804 33.32566755602676 20.342406885167303 74-75 17.324212659806673 34.24559557218584 28.108727003429994 20.321464764577488 76-77 18.739650539734118 28.491673566306847 28.43670426319654 24.3319716307625 78-79 19.175516582435172 29.368356466510786 29.849261230784542 21.606865720269504 80-81 22.37314065491822 27.93716838735767 27.921967190750657 21.76772376697345 82-83 20.306263642033056 26.749493295915183 31.929470689117558 21.014772372934207 84-85 18.514209318007303 29.399996881784556 30.408252357175986 21.67754144303215 86-87 18.77717669669904 30.68941127116453 28.84434710074585 21.68906493139058 88-89 18.009959903596133 30.372413360502055 26.464998711152017 25.152628024749802 90-91 19.81643215662892 26.299661308531803 28.32418589420901 25.559720640630267 92-93 22.490567365282626 29.548455060983535 28.228912283581987 19.73206529015185 94-95 20.56128862513521 27.55469153487103 29.561688153496846 22.322331686496916 96-97 16.347756094803948 28.410098596572603 33.107321950259305 22.13482335836414 98-99 19.28171546343531 30.574890737140603 26.898688845687225 23.244704953736864 100-101 17.233855958215994 27.714157644972374 29.09006752872163 25.96191886809 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1035.0 1 620.0 2 345.5 3 478.0 4 310.5 5 235.0 6 531.5 7 861.5 8 1001.0 9 908.5 10 605.0 11 401.5 12 387.0 13 394.5 14 396.5 15 395.0 16 412.0 17 472.5 18 551.0 19 610.5 20 682.5 21 785.0 22 990.5 23 1211.0 24 1332.5 25 1771.0 26 2892.5 27 4198.5 28 4660.5 29 5185.5 30 6907.0 31 8670.0 32 11412.5 33 14724.0 34 17199.5 35 19556.0 36 22780.5 37 25457.0 38 24045.0 39 21197.0 40 18791.5 41 15988.5 42 13049.0 43 12988.5 44 13660.5 45 12729.0 46 13090.0 47 30000.5 48 63663.0 49 57792.5 50 25818.5 51 12608.0 52 6296.5 53 2868.0 54 1224.5 55 816.0 56 751.5 57 715.5 58 655.5 59 588.5 60 548.5 61 484.5 62 413.5 63 374.5 64 317.0 65 260.5 66 183.5 67 159.5 68 101.0 69 28.0 70 15.0 71 11.5 72 10.5 73 8.5 74 4.5 75 1.5 76 3.0 77 2.5 78 0.5 79 1.0 80 1.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2669940754599314 2 0.019488618646710323 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.8721546616775803E-4 12-13 9.74430932335516E-5 14-15 0.0 16-17 6.821016526348613E-4 18-19 0.0 20-21 9.74430932335516E-5 22-23 0.0 24-25 0.0030207358902400996 26-27 0.003215622076707203 28-29 0.003410508263174306 30-31 0.001753975678203929 32-33 0.002241191144371687 34-35 0.0023386342376052385 36-37 1.948861864671032E-4 38-39 9.74430932335516E-5 40-41 6.821016526348613E-4 42-43 0.003702837542874961 44-45 0.006431244153414406 46-47 0.016175553476769567 48-49 0.01695509822263798 50-51 0.007113345806049268 52-53 0.0090622076707203 54-55 2.923292797006548E-4 56-57 0.0038002806361085127 58-59 0.0018514187714374806 60-61 0.0018514187714374806 62-63 0.0 64-65 6.821016526348613E-4 66-67 0.0 68-69 0.0 70-71 6.821016526348613E-4 72-73 0.003313065169940754 74-75 0.0 76-77 0.19800436545057684 78-79 0.1061155285313377 80-81 4.8721546616775803E-4 82-83 0.0 84-85 0.0011693171188026192 86-87 0.12034222014343622 88-89 0.5796889616463985 90-91 0.31035625194886185 92-93 0.2856057062675397 94-95 0.002923292797006548 96-97 0.003702837542874961 98-99 0.004384939195509822 100-101 9.744309323355161E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 513120.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.849391955098223 #Duplication Level Percentage of deduplicated Percentage of total 1 68.6676798378926 9.510056127221702 2 9.893898457728245 2.7404895541004053 3 4.353821907013397 1.8089335827876518 4 2.7144545761567036 1.5037418147801684 5 1.9405043341213555 1.3437402556906766 6 1.438140267927502 1.195042095416277 7 1.1046380727231793 1.0708995946367321 8 0.9188900146346956 1.0180854381041473 9 0.7261060452549815 0.9050514499532274 >10 6.318248339524936 18.435063922669162 >50 1.0947877969154565 10.589725600249453 >100 0.7556568726781493 18.812948238228874 >500 0.0351795564561522 3.107460243217961 >1k 0.02251491613193741 7.98643592142189 >5k 0.011257458065968704 9.922240411599626 >10k+ 0.0042215467747382645 10.050085749922046 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 27202 5.3012940442781415 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 13938 2.7163236669784845 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10429 2.032468038665419 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 8031 1.5651309635173059 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7413 1.444691300280636 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6445 1.2560414717804802 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6218 1.2118023074524478 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5997 1.1687324602432179 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5846 1.1393046460866854 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5493 1.0705098222637979 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5470 1.0660274399750547 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4064 0.7920174618023074 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3796 0.7397879638291238 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3707 0.7224430932335516 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3653 0.711919239164328 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3489 0.6799579045837231 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3424 0.6672903024633614 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2462 0.4798097910820081 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2397 0.4671421889616464 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2262 0.4408325537885875 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2254 0.4392734642968506 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2182 0.42524165887121923 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2012 0.3921110071718117 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1709 0.3330604926722794 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1245 0.2426333021515435 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1228 0.23932023698160274 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1096 0.2135952603679451 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 983 0.19157312129716247 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 959 0.18689585282195198 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 936 0.1824134705332086 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 799 0.15571406298721546 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 727 0.14168225756158404 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 712 0.1387589647645775 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 655 0.1276504521359526 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 648 0.12628624883068287 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 639 0.12453227315247896 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 607 0.11829591518553165 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 597 0.11634705332086062 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 592 0.1153726223885251 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 587 0.1143981914561896 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 580 0.11303398815091988 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 578 0.11264421577798565 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 574 0.11186467103211724 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 574 0.11186467103211724 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 551 0.10738228874337388 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 547 0.10660274399750547 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 546 0.10640785781103836 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 520 0.10134081696289367 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.9488618646710322E-4 0.0 14-15 0.0 0.0 0.0 1.9488618646710322E-4 0.0 16-17 0.0 0.0 0.0 5.846585594013096E-4 0.0 18-19 0.0 0.0 0.0 9.744309323355161E-4 0.0 20-21 0.0 0.0 0.0 0.002728406610539445 0.0 22-23 0.0 0.0 0.0 0.005261927034611787 0.0 24-25 0.0 0.0 0.0 0.009549423136888058 0.0 26-27 0.0 0.0 0.0 0.01520112254443405 0.0 28-29 0.0 0.0 0.0 0.03147411911443717 0.0 30-31 0.0 0.0 0.0 0.07980589335827876 0.0 32-33 0.0 0.0 0.0 0.1997583411287808 0.0 34-35 0.0 0.0 0.0 0.35040536326785154 0.0 36-37 0.0 0.0 0.0 0.5394449641409417 0.0 38-39 0.0 0.0 0.0 0.804295291549735 0.0 40-41 0.0 0.0 0.0 1.0923370751481136 0.0 42-43 0.0 0.0 0.0 1.3859331150608045 0.0 44-45 0.0 0.0 0.0 1.6925865294667914 0.0 46-47 0.0 0.0 0.0 2.105160586217649 0.0 48-49 0.0 0.0 0.0 2.464725600249454 0.0 50-51 0.0 0.0 0.0 2.820295447458684 0.0 52-53 0.0 0.0 0.0 3.1364982850015592 0.0 54-55 0.0 0.0 0.0 3.490703928905519 0.0 56-57 0.0 0.0 0.0 4.008419083255379 0.0 58-59 0.0 0.0 0.0 4.423136888057375 0.0 60-61 0.0 0.0 0.0 4.863871998752728 0.0 62-63 0.0 0.0 0.0 5.260075615840349 0.0 64-65 0.0 0.0 0.0 5.65793576551294 0.0 66-67 0.0 0.0 0.0 6.158988150919862 0.0 68-69 0.0 0.0 0.0 6.7562168693483 0.0 70-71 0.0 0.0 0.0 7.34993763642033 0.0 72-73 0.0 0.0 0.0 7.838419862800125 0.0 74-75 0.0 0.0 0.0 8.414600873090116 0.0 76-77 0.0 0.0 0.0 8.931828811973809 0.0 78-79 0.0 0.0 0.0 9.807744777050203 0.0 80-81 0.0 0.0 0.0 10.65871531025881 0.0 82-83 0.0 0.0 0.0 11.299988306828812 0.0 84-85 0.0 0.0 0.0 11.990080293108825 0.0 86-87 0.0 0.0 0.0 12.68104926722794 0.0 88-89 0.0 0.0 0.0 13.514285157468038 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACATAAG 15 6.6531217E-4 94.86572 3 GTAGTAA 20 0.0020602106 71.364876 1 TACTCCC 40 7.6030283E-6 59.291077 5 GTTGCCT 35 2.8060193E-4 54.208984 9 GGGTTGC 40 5.4230593E-4 47.43286 7 CCCGCTG 55 5.001023E-5 43.12078 9 GTAAGGT 45 9.687284E-4 42.16254 4 ACTCCCG 60 8.353665E-5 39.52738 6 TATGTCA 30 8.359512E-5 39.52738 34-35 TGGGGGA 85 3.6759957E-7 39.062355 6 GTACATA 50 0.0016022472 38.061268 1 ATGGGGG 140 1.0913936E-11 37.268677 5 TTGCGTT 140 1.0913936E-11 37.268677 4 CTTTGCG 140 1.0913936E-11 37.268677 2 CTCCCGC 65 1.33825E-4 36.486816 7 GTAGATA 80 1.0869315E-5 35.574646 6 GCTTTGC 150 2.3646862E-11 34.889496 1 CGTGCAA 55 0.0025962463 34.496624 9 GACCGTG 55 0.0025962463 34.496624 6 CTGACCG 55 0.0025962463 34.496624 4 >>END_MODULE