FastQCFastQC Report
Thu 26 May 2016
SRR936322_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936322_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235729
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40840.1826697242823258No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28350.12680427726258417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49050.029.5370061
GTATCAA72700.028.9807551
GTACATG106500.027.7408071
TACATGG105500.027.1013432
ACATGGG103200.026.4204033
TCAACGC84150.024.7511394
ATCAACG85500.024.3603323
CAACGCA85550.024.2907625
AACGCAG87050.024.0347966
CATGGGG52900.023.4445694
TATCAAC89600.023.3518142
AGTACAT69400.021.0086232
CATGGGA48700.020.5092664
ACGCAGA102500.020.2734477
CGCAGAG103550.019.9768948
GTACCTG21600.019.2891451
GAGTGCA11150.019.1044399
GTACTAG2251.7217299E-618.9384351
GTACTTT43600.018.51161214-15
TAGACTG6950.018.389745
Produced by FastQC (version 0.11.5)