##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936322_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2235729 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.392073010637695 33.0 31.0 34.0 28.0 34.0 2 31.48688280198539 33.0 31.0 34.0 28.0 34.0 3 31.55274901385633 34.0 31.0 34.0 28.0 34.0 4 35.05763041942919 37.0 35.0 37.0 32.0 37.0 5 35.01805272463702 37.0 35.0 37.0 32.0 37.0 6 35.10100329691121 37.0 35.0 37.0 32.0 37.0 7 35.03273742032241 37.0 35.0 37.0 32.0 37.0 8 35.03177084521425 37.0 35.0 37.0 32.0 37.0 9 36.65104133819439 39.0 37.0 39.0 32.0 39.0 10-11 36.5547302468233 39.0 37.0 39.0 32.0 39.0 12-13 36.44727424477654 39.0 37.0 39.0 32.0 39.0 14-15 37.63536569056447 40.0 38.0 41.0 32.0 41.0 16-17 37.573121339840384 40.0 38.0 41.0 32.0 41.0 18-19 37.50779432569868 40.0 38.0 41.0 32.0 41.0 20-21 37.41621658975663 40.0 37.5 41.0 32.0 41.0 22-23 37.24711246309369 40.0 37.0 41.0 31.5 41.0 24-25 37.38432676768964 40.0 37.5 41.0 32.0 41.0 26-27 37.32491885197177 40.0 37.0 41.0 31.5 41.0 28-29 37.18278445196175 40.0 37.0 41.0 31.0 41.0 30-31 37.027650712586365 40.0 37.0 41.0 31.0 41.0 32-33 36.84809742146744 40.0 36.5 41.0 30.0 41.0 34-35 36.586479846170974 40.0 36.0 41.0 30.0 41.0 36-37 36.391699307026926 40.0 36.0 41.0 30.0 41.0 38-39 36.21071068989131 40.0 35.5 41.0 29.5 41.0 40-41 36.01128602795777 39.0 35.0 41.0 28.5 41.0 42-43 35.809811922643576 39.0 35.0 41.0 28.0 41.0 44-45 35.61671741074164 39.0 35.0 41.0 27.0 41.0 46-47 35.32896898506036 39.0 35.0 41.0 26.5 41.0 48-49 35.15504115212532 39.0 35.0 40.5 26.0 41.0 50-51 34.834333454546595 38.5 34.5 40.5 25.5 41.0 52-53 34.847374167441586 38.5 34.5 40.0 26.0 41.0 54-55 34.90754425066723 39.0 35.0 40.5 26.0 41.0 56-57 34.720047912783706 38.0 34.0 40.0 25.5 41.0 58-59 34.45713590511194 38.0 34.0 40.0 25.0 41.0 60-61 34.12923726444484 37.0 34.0 40.0 24.0 41.0 62-63 33.78972943500756 37.0 34.0 40.0 23.0 41.0 64-65 33.430099533530225 36.0 33.0 39.5 22.5 41.0 66-67 33.049430856780944 36.0 33.0 39.0 22.0 41.0 68-69 32.65527485665749 35.0 33.0 39.0 20.5 40.5 70-71 32.246501253058845 35.0 32.0 37.5 20.0 40.0 72-73 31.846027179501633 35.0 32.0 37.0 18.5 39.5 74-75 31.442070349313354 35.0 32.0 36.5 17.5 39.0 76-77 31.047271382175566 35.0 31.0 36.0 13.5 39.0 78-79 30.679115178986365 35.0 31.0 35.5 9.5 37.5 80-81 30.312055933433793 34.5 31.0 35.0 7.0 37.0 82-83 29.988520299195475 34.0 30.5 35.0 4.0 36.5 84-85 29.6905644646556 34.0 30.0 35.0 2.0 36.0 86-87 29.3713728273865 34.0 30.0 35.0 2.0 36.0 88-89 29.04843028828628 34.0 29.5 35.0 2.0 35.5 90-91 28.69175557502721 34.0 29.0 35.0 2.0 35.0 92-93 28.377489624189693 34.0 29.0 35.0 2.0 35.0 94-95 27.98354183355854 34.0 29.0 35.0 2.0 35.0 96-97 27.529341883564598 34.0 27.0 35.0 2.0 35.0 98-99 26.987946437157635 34.0 27.0 35.0 2.0 35.0 100-101 23.805342463241296 29.0 13.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5411662951491643 1101 2 0.5808661807055948 1101 3 0.6673514710333386 1101 4 0.43454589154771384 1101 5 0.644911090386266 1101 6 0.4469092219750763 1101 7 0.6082269869815917 1101 8 0.5809709936007508 1101 9 0.6717250821835705 1101 10-11 0.8133208605201787 1101 12-13 0.7223976908653711 1101 14-15 0.9357338695811066 1101 16-17 0.9145430675622634 1101 18-19 0.9863630043007063 1101 20-21 1.096108856776688 1101 22-23 1.0220219357845934 1101 24-25 1.0386933130474603 1101 26-27 0.9107799980450366 1101 28-29 0.9435975552286493 1101 30-31 1.0306493674516517 1101 32-33 0.9889199408031928 1101 34-35 0.846266634296569 1101 36-37 0.8821292612335938 1101 38-39 1.0182680171766023 1101 40-41 0.9205673662706673 1101 42-43 0.7955305447230003 1101 44-45 0.7950556111318363 1101 46-47 0.8067180680152859 1101 48-49 0.9425987877760704 1101 50-51 0.9455577594235578 1101 52-53 1.0086720138041585 1101 54-55 0.8718368139647978 1101 56-57 0.8884953893427081 1101 58-59 1.020393727282638 1101 60-61 0.8872592254454403 1101 62-63 0.8683127347689634 1101 64-65 0.7591456564862753 1101 66-67 0.9455536940138245 1101 68-69 1.0062930440079683 1101 70-71 1.070308453139937 1101 72-73 1.079405176464551 1101 74-75 0.9143806713545963 1101 76-77 0.9671486054333407 1101 78-79 0.9993138032991951 1101 80-81 1.073466252646579 1101 82-83 1.1310347612074736 1101 84-85 1.0999416139284648 1101 86-87 1.0344825005666252 1101 88-89 0.9457429678755531 1101 90-91 1.151326478703485 1101 92-93 1.092813859826979 1101 94-95 1.4168690007598705 1101 96-97 1.4936947149996485 1101 98-99 1.5938947451500276 1101 100-101 1.5549853039477846 1102 1 0.4345544376205872 1102 2 0.42378734069762203 1102 3 0.413788068663127 1102 4 0.37431539136716907 1102 5 0.49290869753893674 1102 6 0.4303320176183689 1102 7 0.29397607214863086 1102 8 0.2453000532225289 1102 9 0.4046472122488396 1102 10-11 0.5385593368584765 1102 12-13 0.3988381148951632 1102 14-15 0.5783687232347816 1102 16-17 0.5004632628703405 1102 18-19 0.6012472961202988 1102 20-21 0.5411108238696443 1102 22-23 0.5316747034574405 1102 24-25 0.6486093478191961 1102 26-27 0.5840300937050387 1102 28-29 0.5356209932760052 1102 30-31 0.5335730604126994 1102 32-33 0.3329964310782074 1102 34-35 0.37399806413063175 1102 36-37 0.39722535237735457 1102 38-39 0.3752529487056009 1102 40-41 0.5130304361213263 1102 42-43 0.32910168073265567 1102 44-45 0.33664103416460733 1102 46-47 0.3727582290474416 1102 48-49 0.3451243465113194 1102 50-51 0.2671133257577054 1102 52-53 0.2016300904722783 1102 54-55 0.2702938585827681 1102 56-57 0.33346017029478503 1102 58-59 0.4239049882161723 1102 60-61 0.3856774671337959 1102 62-63 0.5430718332475664 1102 64-65 0.3604832796990536 1102 66-67 0.41043758163622357 1102 68-69 0.5038615284841015 1102 70-71 0.6275232381339819 1102 72-73 0.5685889945650366 1102 74-75 0.6186326909931914 1102 76-77 0.5013942118099557 1102 78-79 0.5251948184269501 1102 80-81 0.5388089040723152 1102 82-83 0.5734644174242973 1102 84-85 0.4773944171706681 1102 86-87 0.5890317398675258 1102 88-89 0.683799749495897 1102 90-91 0.7981273927280519 1102 92-93 0.5927361192353402 1102 94-95 0.5169494359994005 1102 96-97 0.5562214593799588 1102 98-99 0.4666635766968099 1102 100-101 0.4309866351038387 1103 1 0.3860305599783054 1103 2 0.3946592584144888 1103 3 0.40796229529739847 1103 4 0.2608002875587303 1103 5 0.3342461384038202 1103 6 0.45206340493017905 1103 7 0.4728767669944247 1103 8 0.47924106063125294 1103 9 0.4826726158321222 1103 10-11 0.5686912451084964 1103 12-13 0.5002047931556675 1103 14-15 0.5307290283706791 1103 16-17 0.7107083810359711 1103 18-19 0.5748689150560935 1103 20-21 0.6843889687856617 1103 22-23 0.7042540536968573 1103 24-25 0.8668548687181854 1103 26-27 0.8415674838359806 1103 28-29 0.7608511920013754 1103 30-31 0.6428929379189938 1103 32-33 0.7386968079215634 1103 34-35 0.5174865979981504 1103 36-37 0.5677371637098574 1103 38-39 0.6839421823877103 1103 40-41 0.6247720748276606 1103 42-43 0.4235605666257385 1103 44-45 0.5228366945819332 1103 46-47 0.5476104075995423 1103 48-49 0.6254011341636172 1103 50-51 0.45584319889670155 1103 52-53 0.5398658307040378 1103 54-55 0.6137251206288212 1103 56-57 0.6594389561844665 1103 58-59 0.7358209267810238 1103 60-61 0.7127047980838555 1103 62-63 0.7264905948686504 1103 64-65 0.6563563088553792 1103 66-67 0.6762464349706789 1103 68-69 0.6128367290008541 1103 70-71 0.6287327457793808 1103 72-73 0.6818332994185035 1103 74-75 0.7254137792624071 1103 76-77 0.5188346582686485 1103 78-79 0.6094721343398852 1103 80-81 0.5182409977365872 1103 82-83 0.5909614819165512 1103 84-85 0.725941046632439 1103 86-87 0.7873240458298092 1103 88-89 0.7628731751092133 1103 90-91 0.8988397600417919 1103 92-93 0.76146099743465 1103 94-95 0.7129919269836655 1103 96-97 0.8770996534608244 1103 98-99 0.8424863687896398 1103 100-101 1.0579713390817496 1104 1 0.31249415069795816 1104 2 0.18505926415727814 1104 3 0.2994523028450651 1104 4 0.31590388336485375 1104 5 0.2871844977711291 1104 6 0.3074497305298891 1104 7 0.3613251475280137 1104 8 0.3203334826113675 1104 9 0.40473169271985654 1104 10-11 0.3911019139792984 1104 12-13 0.4058030891882183 1104 14-15 0.5910001085071954 1104 16-17 0.6063780619830368 1104 18-19 0.47717049145158796 1104 20-21 0.6041078592788836 1104 22-23 0.6164445071310354 1104 24-25 0.5813152106602288 1104 26-27 0.5180479952723331 1104 28-29 0.6676726507517472 1104 30-31 0.6733338200113366 1104 32-33 0.7357103014240138 1104 34-35 0.7678114244838952 1104 36-37 0.7145454037912415 1104 38-39 0.7010003158173035 1104 40-41 0.7384331436426805 1104 42-43 0.8251184006099237 1104 44-45 0.8690278925276615 1104 46-47 0.8654045409306832 1104 48-49 0.642417991299439 1104 50-51 0.6658790297961801 1104 52-53 0.609329474749579 1104 54-55 0.8376841376274697 1104 56-57 0.8391028697308869 1104 58-59 0.8787543227381036 1104 60-61 0.769365868949869 1104 62-63 0.8460180248886644 1104 64-65 0.8247274386259988 1104 66-67 0.9501871981052545 1104 68-69 0.865158200057202 1104 70-71 0.6462741451329066 1104 72-73 0.9145205861149215 1104 74-75 0.8443751343964543 1104 76-77 0.7826617039320176 1104 78-79 0.7526544699320468 1104 80-81 0.8397035937231969 1104 82-83 1.0058297669557668 1104 84-85 0.9084035441712466 1104 86-87 0.9287102217068153 1104 88-89 0.9073350758081951 1104 90-91 0.8739127242231994 1104 92-93 0.8045211449422816 1104 94-95 0.5462880005569914 1104 96-97 0.6995469838844812 1104 98-99 0.8210183376637197 1104 100-101 1.00435753546871 1105 1 0.4584618926334407 1105 2 0.45874878028631016 1105 3 0.3892385733043078 1105 4 0.2870409602757036 1105 5 0.3691202795922024 1105 6 0.34969706884230334 1105 7 0.5883015498798372 1105 8 0.5582928223539341 1105 9 0.4162077375315505 1105 10-11 0.4847672464916144 1105 12-13 0.4647162665281215 1105 14-15 0.6000771584038986 1105 16-17 0.5843141119592019 1105 18-19 0.59527518118621 1105 20-21 0.6298795500464038 1105 22-23 0.5892101296481229 1105 24-25 0.7001694931963769 1105 26-27 0.5725962035990406 1105 28-29 0.6742491544066027 1105 30-31 0.8230040885636996 1105 32-33 0.730863894702253 1105 34-35 0.6283581132650795 1105 36-37 0.8501981131508458 1105 38-39 0.7746913216968494 1105 40-41 0.8121072656830464 1105 42-43 0.9589521245889401 1105 44-45 0.9505316466989626 1105 46-47 0.9524332831839857 1105 48-49 0.9468497507941152 1105 50-51 0.9284351437323011 1105 52-53 0.9926358270457811 1105 54-55 0.9247108935467452 1105 56-57 1.0252573767227773 1105 58-59 0.985687125058142 1105 60-61 0.9542098920708 1105 62-63 0.6359080813005562 1105 64-65 0.6857786562487433 1105 66-67 0.7120401497976161 1105 68-69 0.7135693811314141 1105 70-71 0.8536461755635472 1105 72-73 0.8784589402385485 1105 74-75 0.8883649842296073 1105 76-77 0.8277482584370297 1105 78-79 0.805222551530651 1105 80-81 0.8662988682345585 1105 82-83 0.9236739986417675 1105 84-85 0.9253213993277676 1105 86-87 0.895086800842364 1105 88-89 0.9601417657811808 1105 90-91 0.9889564732300329 1105 92-93 1.0793210217895712 1105 94-95 1.173134298030373 1105 96-97 1.2846276290457723 1105 98-99 1.1466482538404286 1105 100-101 1.0596498741783904 1106 1 0.29134520989477153 1106 2 0.2687777098909372 1106 3 0.25188565587956546 1106 4 0.31526181334276515 1106 5 0.231439113287351 1106 6 0.2864504926538842 1106 7 0.2684840581573127 1106 8 0.22387181822588786 1106 9 0.27013223553878163 1106 10-11 0.3623154506234627 1106 12-13 0.3609772695160842 1106 14-15 0.3785915828282711 1106 16-17 0.3160853733184581 1106 18-19 0.2334985994398906 1106 20-21 0.2011843749724207 1106 22-23 0.3109151711898903 1106 24-25 0.3805655295080257 1106 26-27 0.30218658129901854 1106 28-29 0.3636972117527719 1106 30-31 0.4301968243352192 1106 32-33 0.3719892990754232 1106 34-35 0.2664580225334774 1106 36-37 0.3356278531644321 1106 38-39 0.33223534552458744 1106 40-41 0.2274824717292816 1106 42-43 0.12155692908847726 1106 44-45 0.23405174778631732 1106 46-47 0.3571674775966258 1106 48-49 0.3067264318226606 1106 50-51 0.27034007981639974 1106 52-53 0.23557230067424229 1106 54-55 0.18532981607335586 1106 56-57 0.28394195852610693 1106 58-59 0.25774915507081886 1106 60-61 0.3409561903639968 1106 62-63 0.24980521731719563 1106 64-65 0.15963014144429621 1106 66-67 0.14345513523780085 1106 68-69 0.18281493124518278 1106 70-71 0.1773406618374267 1106 72-73 0.23121105295474464 1106 74-75 0.11867123900964316 1106 76-77 0.10494707465090514 1106 78-79 0.13234702483608274 1106 80-81 0.16201666920457924 1106 82-83 0.21058157116766552 1106 84-85 0.3395059312524644 1106 86-87 0.20249330216533323 1106 88-89 -0.020311749712071503 1106 90-91 0.12799528830382556 1106 92-93 0.08141663674556199 1106 94-95 0.14527565881437354 1106 96-97 0.14441547861182613 1106 98-99 0.2826717870524682 1106 100-101 0.18660262249082749 1107 1 0.2685845920199448 1107 2 0.3800166739468409 1107 3 0.4463864673677911 1107 4 0.3015508175293746 1107 5 0.3056646498260136 1107 6 0.5021978999342025 1107 7 0.36588926785257314 1107 8 0.458130129817647 1107 9 0.5164248288106279 1107 10-11 0.40987428105496093 1107 12-13 0.5503902568773782 1107 14-15 0.6551956771462812 1107 16-17 0.5011512783891305 1107 18-19 0.6357453273576468 1107 20-21 0.7893587828226529 1107 22-23 0.7890683403324417 1107 24-25 0.8545027560128275 1107 26-27 0.6577350211315576 1107 28-29 0.7210779091790798 1107 30-31 0.7298349084736273 1107 32-33 0.7783824557547732 1107 34-35 0.7303517264497188 1107 36-37 0.869581191112303 1107 38-39 0.7937628661174543 1107 40-41 0.9676760851295896 1107 42-43 0.5819078903519923 1107 44-45 0.8210520613382712 1107 46-47 0.8879581806826451 1107 48-49 0.8612107670432252 1107 50-51 0.950869588210324 1107 52-53 0.9257679354000246 1107 54-55 1.0292174264328011 1107 56-57 0.9576231176034327 1107 58-59 1.1238634575754887 1107 60-61 1.0992034448123889 1107 62-63 1.235595792221602 1107 64-65 0.9055473104842946 1107 66-67 0.8604663639473245 1107 68-69 0.9826373997075777 1107 70-71 1.0800784239441725 1107 72-73 0.9261097204575499 1107 74-75 0.8178304662125377 1107 76-77 0.9000739054294833 1107 78-79 0.8532594117158077 1107 80-81 0.8445640520317923 1107 82-83 0.9763856248619582 1107 84-85 0.9740364808755153 1107 86-87 0.7979437422226816 1107 88-89 1.0005429061400122 1107 90-91 1.2713844200804445 1107 92-93 1.0043230365578246 1107 94-95 1.0753074774354126 1107 96-97 1.4317646433606974 1107 98-99 1.4144565385674746 1107 100-101 1.5221754365505795 1108 1 -0.14830846418265864 1108 2 -0.1537855289543124 1108 3 -0.11832726212852052 1108 4 -0.07889632986892536 1108 5 -0.05645897757048601 1108 6 -0.12542405573460513 1108 7 -0.10860261860839415 1108 8 -0.14269600488974277 1108 9 0.017702245799569027 1108 10-11 -0.19104727403534127 1108 12-13 -0.27636468299842676 1108 14-15 -0.3167315526503174 1108 16-17 -0.11744292678714174 1108 18-19 -0.162612225914053 1108 20-21 -0.16216228356561402 1108 22-23 -0.059345389350966116 1108 24-25 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0.921335588874733 2204 64-65 0.7540330099572046 2204 66-67 0.9320302050717828 2204 68-69 0.8922668533208693 2204 70-71 0.9481002563702248 2204 72-73 1.0771869404235623 2204 74-75 1.0869727226139325 2204 76-77 0.9406914347362054 2204 78-79 0.8404083791399835 2204 80-81 0.832174582289003 2204 82-83 0.9473042861137237 2204 84-85 1.0618543903775155 2204 86-87 1.0387499290368183 2204 88-89 1.0239695601335015 2204 90-91 0.9819211578897118 2204 92-93 0.8815898069141106 2204 94-95 0.9860890379642306 2204 96-97 1.0585712910176 2204 98-99 1.3813183433429579 2204 100-101 1.2049550384976406 2205 1 0.5279796360365374 2205 2 0.4978752589032318 2205 3 0.5719030645345775 2205 4 0.5678856152912317 2205 5 0.5731019557241126 2205 6 0.5691410775022092 2205 7 0.5215606255285437 2205 8 0.4908867164896691 2205 9 0.6861082740694684 2205 10-11 0.6521702147072332 2205 12-13 0.6643193539743848 2205 14-15 0.7859624459693961 2205 16-17 0.6817428088937163 2205 18-19 0.696787527833628 2205 20-21 0.5973797853700518 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1.127221556186253 2205 90-91 1.1481670408306748 2205 92-93 1.0422230330223563 2205 94-95 1.0043978291401636 2205 96-97 1.032989776452503 2205 98-99 1.2204441811724855 2205 100-101 1.4270748059345344 2206 1 0.34810442242262596 2206 2 0.4816635373898883 2206 3 0.5191419926827443 2206 4 0.4954045079767795 2206 5 0.39545441513016755 2206 6 0.3695875295305129 2206 7 0.4110889776927067 2206 8 0.4447444439963064 2206 9 0.5158451651186624 2206 10-11 0.4346184698913049 2206 12-13 0.5707750186107674 2206 14-15 0.510075442619133 2206 16-17 0.46258911613578135 2206 18-19 0.6021356111257532 2206 20-21 0.4390486135439815 2206 22-23 0.5733788545039715 2206 24-25 0.5957145483498323 2206 26-27 0.5742201035778223 2206 28-29 0.7902303349568243 2206 30-31 0.8173826358358198 2206 32-33 0.841536979005987 2206 34-35 0.7153479494979393 2206 36-37 0.8386861003998618 2206 38-39 0.8173610827964026 2206 40-41 0.8830409686626339 2206 42-43 0.9028405443963265 2206 44-45 0.913817464834807 2206 46-47 0.8191698605545881 2206 48-49 0.9934750730681614 2206 50-51 1.0461424940639432 2206 52-53 0.9911905041396025 2206 54-55 0.9805978413914076 2206 56-57 0.944012033046576 2206 58-59 0.8792481911926089 2206 60-61 0.8696648233716786 2206 62-63 0.8814901612832102 2206 64-65 0.9462645845560331 2206 66-67 0.920280206147595 2206 68-69 0.6024194892710071 2206 70-71 0.760779324976788 2206 72-73 0.8510903670941907 2206 74-75 1.0438060537956453 2206 76-77 0.9345974003188822 2206 78-79 1.1431041443207413 2206 80-81 1.0802856747527336 2206 82-83 1.146270432518584 2206 84-85 0.9775758502089111 2206 86-87 0.9823845752889078 2206 88-89 1.1001091658705846 2206 90-91 1.1797916991943715 2206 92-93 1.062641561990862 2206 94-95 0.8961325867608316 2206 96-97 0.8298155300540202 2206 98-99 0.9796949457622439 2206 100-101 0.8247277843525431 2207 1 0.5612915474155606 2207 2 0.6896486105070245 2207 3 0.5874404182949782 2207 4 0.5679819110954654 2207 5 0.5710784564581743 2207 6 0.5180821583736943 2207 7 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0.09574759127376353 2312 28-29 -0.27175992368042756 2312 30-31 -0.21491127451258052 2312 32-33 -0.16202978330132112 2312 34-35 -0.38299921934824965 2312 36-37 -0.3437863325061912 2312 38-39 -0.3086738579484489 2312 40-41 -0.19614080324093663 2312 42-43 -0.4541778706718702 2312 44-45 -0.2542753212237514 2312 46-47 -0.38704336736442 2312 48-49 -0.21657516900793183 2312 50-51 -0.2315906857665766 2312 52-53 -0.4640500103454386 2312 54-55 -0.3768266980035335 2312 56-57 -0.3696183565763107 2312 58-59 -0.30772814644425495 2312 60-61 -0.3219038634590419 2312 62-63 -0.17892065666030987 2312 64-65 -0.1137861318001967 2312 66-67 -0.14950590053592805 2312 68-69 -0.26595828645350394 2312 70-71 -0.13121628424307374 2312 72-73 -0.2728968252341346 2312 74-75 -0.3441226910588533 2312 76-77 -0.3410983071648026 2312 78-79 -0.4767374882709454 2312 80-81 -0.5496821901329341 2312 82-83 -0.7242944772856319 2312 84-85 -0.5257497318645612 2312 86-87 -0.5483400826058009 2312 88-89 -0.7546669580943011 2312 90-91 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68-69 -0.8016198455655932 2314 70-71 -0.7195058091019249 2314 72-73 -0.8747104189687818 2314 74-75 -0.8574689303688139 2314 76-77 -0.8317754001088353 2314 78-79 -0.7008434101294085 2314 80-81 -0.6725932918431283 2314 82-83 -0.6713734416479653 2314 84-85 -0.7695288335708064 2314 86-87 -0.7765748503369885 2314 88-89 -0.5416373583172529 2314 90-91 -0.6042719847839102 2314 92-93 -0.6475579742191755 2314 94-95 -0.7740062400912571 2314 96-97 -0.6668435000458075 2314 98-99 -0.5473466588067559 2314 100-101 -0.4936601662776603 2315 1 -0.804948409320378 2315 2 -0.6765975785058735 2315 3 -0.7054967030564967 2315 4 -0.6233248667067741 2315 5 -0.8858718893829405 2315 6 -0.7379027876618238 2315 7 -0.9014341202196476 2315 8 -0.7582459521562939 2315 9 -0.9427060686965163 2315 10-11 -0.9966395380633344 2315 12-13 -1.018203349532918 2315 14-15 -1.08812686823034 2315 16-17 -1.0801177231255394 2315 18-19 -1.191793565587325 2315 20-21 -1.2303887172409134 2315 22-23 -1.2125074459715819 2315 24-25 -1.3548161134674288 2315 26-27 -1.4530230598471334 2315 28-29 -1.3042549011018068 2315 30-31 -1.3163312805444392 2315 32-33 -1.608007737738312 2315 34-35 -1.3325204489762612 2315 36-37 -1.138851576670568 2315 38-39 -1.6522233420065717 2315 40-41 -1.2938775622531509 2315 42-43 -1.2973997552945065 2315 44-45 -1.2954780305228155 2315 46-47 -1.1146449133178677 2315 48-49 -1.429780108597427 2315 50-51 -1.5032013217193168 2315 52-53 -1.6544863806907415 2315 54-55 -1.448665809373047 2315 56-57 -1.4663599267797451 2315 58-59 -1.5166302458174457 2315 60-61 -1.452949152106683 2315 62-63 -1.4391218865878699 2315 64-65 -1.3634675529227422 2315 66-67 -1.5950047101626659 2315 68-69 -1.2714178004871144 2315 70-71 -1.3253522407319878 2315 72-73 -1.4569827908116828 2315 74-75 -1.5369029768165134 2315 76-77 -1.4805141290193333 2315 78-79 -1.431269586820374 2315 80-81 -1.3588610334182682 2315 82-83 -1.8134538089337653 2315 84-85 -1.2718633691101395 2315 86-87 -1.3266558865553755 2315 88-89 -1.4328750599850792 2315 90-91 -1.527290059682148 2315 92-93 -1.6490146010612712 2315 94-95 -1.4695176632257407 2315 96-97 -1.3746086054124902 2315 98-99 -1.405393937446572 2315 100-101 -1.507934495270991 2316 1 -1.2520276178560685 2316 2 -1.1346078630703325 2316 3 -1.2081554120207123 2316 4 -1.0701432348395272 2316 5 -1.2154244859870431 2316 6 -1.0899081535215416 2316 7 -1.0563124568031412 2316 8 -1.2547475855140604 2316 9 -1.27380569730947 2316 10-11 -1.3095427538528668 2316 12-13 -1.3657222604621282 2316 14-15 -1.6897726469631422 2316 16-17 -1.7642580055784336 2316 18-19 -1.617172715744438 2316 20-21 -1.716412950556439 2316 22-23 -1.6646162164196028 2316 24-25 -1.73018377422585 2316 26-27 -1.6749286853051544 2316 28-29 -1.9497044781677246 2316 30-31 -1.7405788801916842 2316 32-33 -1.749690034127532 2316 34-35 -1.7743521075485873 2316 36-37 -1.6544231276772834 2316 38-39 -1.6749555831224683 2316 40-41 -1.61018940316535 2316 42-43 -1.7248752549280866 2316 44-45 -1.8481240079911743 2316 46-47 -1.8723161053585144 2316 48-49 -1.9690679521925532 2316 50-51 -1.8540108585412867 2316 52-53 -1.9200334002786121 2316 54-55 -1.9752761883368848 2316 56-57 -2.047026374009249 2316 58-59 -2.1682701245887657 2316 60-61 -2.0946472852346574 2316 62-63 -2.102562930446325 2316 64-65 -1.8672217915792828 2316 66-67 -1.9405632812541 2316 68-69 -2.051293342351997 2316 70-71 -1.861824560596844 2316 72-73 -1.8423409495268608 2316 74-75 -1.6894020762960729 2316 76-77 -1.9716669814954564 2316 78-79 -2.10399064906186 2316 80-81 -1.9799540168114298 2316 82-83 -2.184189689394337 2316 84-85 -2.26253987732661 2316 86-87 -2.3182102469374435 2316 88-89 -2.177773766961934 2316 90-91 -2.388028610651606 2316 92-93 -2.4327384704423345 2316 94-95 -2.33250316795192 2316 96-97 -2.7578548884367464 2316 98-99 -2.5114522663290586 2316 100-101 -2.313077398548881 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27138.0 3 5959.0 4 4794.0 5 3917.0 6 3735.0 7 4003.0 8 4689.0 9 5520.0 10 6482.0 11 7409.0 12 9205.0 13 11862.0 14 11703.0 15 10560.0 16 12354.0 17 14749.0 18 13695.0 19 11176.0 20 11102.0 21 11613.0 22 12559.0 23 13857.0 24 16214.0 25 18918.0 26 22109.0 27 26695.0 28 32041.0 29 38529.0 30 46977.0 31 57911.0 32 73247.0 33 96587.0 34 133239.0 35 199942.0 36 324998.0 37 485126.0 38 390032.0 39 55067.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.79472290886179 17.290250752220864 12.395311082034585 24.519715256882755 2 16.482820414292636 18.72655138308537 38.487693655884016 26.302934546737983 3 20.182472984899636 22.795882280053448 27.58159327473072 29.440051460316198 4 13.20571320450547 14.300008990903084 34.15344385136292 38.34083395322851 5 14.829780067446519 36.78693690006347 32.88409245335262 15.499190579137384 6 35.95829687799135 33.16147882548493 16.064415471020908 14.815808825502815 7 31.712049725447255 30.76368424913537 18.957424196067695 18.566841829349677 8 28.540608291236886 33.95240794062824 18.289040549916106 19.217943218218767 9 27.616694163412987 15.280735218858593 18.47289274121685 38.62967787651158 10-11 26.989628663481056 25.08358750644658 25.6739445385992 22.25283929147316 12-13 27.038085414174738 21.942480476875083 26.61830132486176 24.401132784088418 14-15 23.954012304329865 23.573860534377488 24.020326947967497 28.45180021332515 16-17 24.283945298677292 26.783409585749634 24.7599804772341 24.172664638338972 18-19 24.240284503293484 25.677630033885418 26.352631990963886 23.729453471857212 20-21 24.827053153926048 24.901706790573513 26.365302743085035 23.905937312415404 22-23 24.447267043848253 24.572159239338536 26.433151362899505 24.547422353913706 24-25 24.409458506074632 24.926083052719594 26.497273942089556 24.167184499116217 26-27 24.155424030825188 25.26885147419601 26.347343825938253 24.228380669040554 28-29 24.000988655071218 25.15373362679837 26.70115775535037 24.144119962780046 30-31 24.124526847589845 25.284383526836628 26.438939154439765 24.15215047113376 32-33 23.909227469923067 25.1112311971635 26.483716361118244 24.495824971795184 34-35 24.22534819672249 25.179785139017476 26.375415451488117 24.219451212771926 36-37 24.53271122522018 25.079092772980715 26.033665370957575 24.35453063084153 38-39 24.2695313588177 25.112372878462057 26.314236983212812 24.30385877950743 40-41 24.172519806449785 25.119598195005388 26.65299423546812 24.054887763076707 42-43 24.379117832354176 25.080817585475213 26.082533245977547 24.457531336193068 44-45 24.036105686074777 25.32842911546777 26.16181357185666 24.473651626600798 46-47 24.348357820807333 25.437473228881213 25.94790548160147 24.266263468709983 48-49 24.59891653975954 25.525211217211663 25.609738556333276 24.266133686695518 50-51 24.480581773587687 25.487748302096463 25.627217344239718 24.404452580076132 52-53 24.505266971433553 25.42673694539255 25.592080585752942 24.475915497420957 54-55 24.494465394078457 25.446423455160804 25.566735601586576 24.492375549174163 56-57 24.24129664296277 25.836619049898175 25.43042381418158 24.49166049295748 58-59 24.39544868695185 25.415355176424274 25.737303186584242 24.451892950039635 60-61 24.465468157725034 25.507591230707355 25.702309612562996 24.32463099900461 62-63 24.32302781676106 25.589164310818298 25.81852530408082 24.269282568339825 64-65 24.448713650202382 25.540679503347896 25.65966944392259 24.350937402527126 66-67 24.372109867658533 25.73367209322023 25.555447967564245 24.338770071556997 68-69 24.176677135007893 25.480451917728953 25.887784625583578 24.455086321679577 70-71 24.34747206581214 25.353644313100173 25.726616920508977 24.572266700578716 72-73 24.19996795759797 25.75845107118469 25.328844700633862 24.71273627058348 74-75 24.20010800589785 25.869053074134165 25.417253121912047 24.51358579805594 76-77 24.367099416129776 25.722602321099863 25.541423687011044 24.36887457575932 78-79 24.208008247418974 25.747703220547002 25.639712005450342 24.40457652658368 80-81 24.200819460317476 25.99856277608024 25.447694352177386 24.352923411424896 82-83 24.428962098588165 25.879785988589354 25.316781379838794 24.37447053298369 84-85 24.092800513411206 25.863022034346955 25.20793288622576 24.83624456601608 86-87 24.33850912904479 25.538427243925298 25.65815389503739 24.46490973199252 88-89 24.338996413547342 25.591625347621612 25.44017087678182 24.629207362049225 90-91 24.11961865540287 25.847740908671966 25.568993384242223 24.463647051682937 92-93 24.149347785726132 25.756990675203724 25.477242291410334 24.61641924765981 94-95 24.20839258011573 25.516885108280047 25.742307505631068 24.532414805973154 96-97 24.338486698746028 25.410876431552794 25.555233563819513 24.695403305881666 98-99 24.01855947944326 25.557111213790044 25.491520489576352 24.93280881719034 100-101 24.318119326646766 25.48502621131876 25.412489059896952 24.78436540213752 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 182.0 1 139.5 2 199.0 3 313.5 4 251.0 5 231.0 6 490.5 7 918.5 8 1407.0 9 1515.0 10 1250.0 11 1182.5 12 1363.0 13 1552.0 14 1703.5 15 1925.5 16 2105.5 17 2229.0 18 2310.0 19 2434.0 20 2448.0 21 2454.5 22 2579.0 23 2802.0 24 3084.5 25 3562.0 26 4434.5 27 5500.5 28 6498.0 29 8309.0 30 11363.0 31 14837.0 32 19402.0 33 22942.0 34 25522.5 35 29145.0 36 34974.5 37 40548.5 38 44176.0 39 47321.5 40 49715.5 41 53177.5 42 55302.0 43 60098.0 44 66180.5 45 69714.0 46 73369.0 47 77022.5 48 84498.5 49 87818.0 50 86384.5 51 87969.0 52 91606.0 53 96306.5 54 100141.5 55 96914.0 56 90208.0 57 84173.0 58 79413.5 59 74921.0 60 66684.5 61 57243.5 62 46807.5 63 36021.0 64 27301.0 65 20733.0 66 14963.0 67 11866.0 68 9072.5 69 6372.5 70 4818.5 71 3267.5 72 2535.0 73 2110.5 74 1663.0 75 1321.5 76 901.0 77 506.5 78 301.0 79 164.5 80 100.0 81 62.0 82 44.5 83 38.0 84 36.0 85 29.0 86 23.5 87 27.0 88 29.5 89 21.5 90 21.0 91 23.5 92 24.0 93 26.0 94 22.0 95 19.5 96 22.0 97 19.5 98 14.5 99 13.5 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04530960594955829 2 0.011450403872741285 3 0.009840190828137042 4 0.006083027057393808 5 0.008051065223021216 6 0.00523319239496379 7 0.004159717031894295 8 0.00840889034404438 9 0.013999907860031338 10-11 0.002146950726138991 12-13 0.007827424522381736 14-15 0.007805060452317789 16-17 0.0174439746498793 18-19 0.011360947592485493 20-21 0.0031756979490805905 22-23 0.008990356165707024 24-25 0.02012766305755304 26-27 0.006910497649759878 28-29 0.016504683707193494 30-31 0.014469553331374242 32-33 0.013194801337729215 34-35 0.2583273733086613 36-37 0.45524301022172187 38-39 0.4518883997121297 40-41 0.47277644115185696 42-43 0.4628020659033363 44-45 0.4788371041391868 46-47 0.48530032038766774 48-49 0.49223318210749156 50-51 0.5018944603751171 52-53 0.4906006049928234 54-55 0.4778530850563731 56-57 0.47266462080153726 58-59 0.47076367484610165 60-61 0.4680352582983 62-63 0.46588830757216104 64-65 0.4808722345150061 66-67 0.4544602677694837 68-69 0.48156552068698844 70-71 0.46358480835557436 72-73 0.4719936986996188 74-75 0.48532268445773163 76-77 0.47308953813275223 78-79 0.4717924220690432 80-81 0.47438665419646125 82-83 0.47474447931748437 84-85 0.4746550230372286 86-87 0.47682433783343153 88-89 0.4905782409227595 90-91 0.5141052426300325 92-93 0.5022970136362681 94-95 0.4729106255722406 96-97 0.44723667313882853 98-99 0.4385370498839528 100-101 0.4460737414955032 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2235729.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.9994131063561 #Duplication Level Percentage of deduplicated Percentage of total 1 65.66479582035407 24.295589071018057 2 14.198446490502187 10.506683743411646 3 6.508856001931046 7.2247155619569625 4 3.6197796692438793 5.357188933453734 5 2.271167411616404 4.201593064804442 6 1.579625552566431 3.5067131023656843 7 1.115738049618602 2.8897157111423084 8 0.8102577137556766 2.398324789908633 9 0.6194311812079588 2.062673114802425 >10 3.256475058391952 21.693419302410284 >50 0.2251591409137326 5.7451649124323865 >100 0.12373253012348467 8.091801424021009 >500 0.005567175363474066 1.3455116912770368 >1k 9.682044110389661E-4 0.680905576995427 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4084 0.1826697242823258 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2835 0.12680427726258417 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.156502420463303E-4 2 0.0 0.0 0.0 0.0 7.60378382174226E-4 3 0.0 0.0 0.0 0.0 7.60378382174226E-4 4 0.0 0.0 0.0 0.0 8.498346624300172E-4 5 0.0 0.0 0.0 0.0 8.945628025579129E-4 6 0.0 0.0 0.0 0.0 0.0011629316433252868 7 0.0 0.0 0.0 0.0 0.0011629316433252868 8 0.0 0.0 0.0 0.0 0.0011629316433252868 9 0.0 0.0 0.0 0.0 0.0012971160637089737 10-11 0.0 0.0 0.0 2.2364070063947822E-5 0.001386572343964765 12-13 0.0 0.0 0.0 4.4728140127895645E-5 0.0032204260892084863 14-15 0.0 0.0 0.0 1.3418442038368693E-4 0.003868984121062973 16-17 0.0 0.0 0.0 6.485580318544869E-4 0.0044057218025977205 18-19 0.0 0.0 0.0 8.498346624300172E-4 0.004606998433173251 20-21 0.0 0.0 0.0 0.0012523879235810781 0.005188464254835894 22-23 0.0 0.0 0.0 0.0023705914267784694 0.005479197165667216 24-25 0.0 0.0 0.0 0.0036006152802955994 0.005792294146562486 26-27 0.0 0.0 0.0 0.003936076331254817 0.006217211477777495 28-29 0.0 0.0 0.0 0.0045622702930453556 0.006396124038289077 30-31 0.0 0.0 0.0 0.0075814197516783114 0.006530308458672764 32-33 0.0 0.0 0.0 0.012479151095682886 0.007134138350399355 34-35 0.0 0.0 0.0 0.01927782839512302 0.007469599401358573 36-37 0.0 0.0 0.0 0.029632392834730865 0.007469599401358573 38-39 0.0 0.0 0.0 0.0472329159750578 0.007559055681614364 40-41 0.0 0.0 0.0 0.0676736760135061 0.007670876031934103 42-43 0.0 0.0 0.0 0.08695150440862913 0.007916880802637529 44-45 0.0 0.0 0.0 0.11074687495666961 0.008520710694364119 46-47 0.0 0.0 0.0 0.13803104043468595 0.009169268726218607 48-49 0.0 0.0 0.0 0.17066021865798583 0.009504729777177826 50-51 0.0 0.0 0.0 0.20689001216158132 0.009974375248520729 52-53 0.0 0.0 0.0 0.24045848132756698 0.010824209910950745 54-55 0.0 0.0 0.0 0.2783655800859586 0.011182035031973911 56-57 0.0 0.0 0.0 0.32405537522660394 0.011472767942805231 58-59 0.0 0.0 0.0 0.37797514815078215 0.011741136783572607 60-61 0.0 0.0 0.0 0.43609936624698253 0.011852957133892346 62-63 0.0 0.0 0.0 0.49590088959797896 0.012613335516066571 64-65 0.0 0.0 0.0 0.5568877086623647 0.012859340286769997 66-67 0.0 0.0 0.0 0.6298616692810265 0.013105345057473423 68-69 0.0 0.0 0.0 0.7150687762246677 0.013328985758112902 70-71 0.0 0.0 0.0 0.8097135207352948 0.013574990528816328 72-73 0.0 0.0 0.0 0.905230463978416 0.014626101821821875 74-75 0.0 0.0 0.0 1.0044151147120246 0.01529702392374031 76-77 0.0 0.0 0.0 1.113954329885241 0.015453572414187945 78-79 4.4728140127895645E-5 0.0 0.0 1.2401100491159707 0.015632484974699528 80-81 4.4728140127895645E-5 0.0 0.0 1.3838439274169634 0.015945581955594798 82-83 4.4728140127895645E-5 0.0 0.0 1.534018657896373 0.01675068847789692 84-85 4.4728140127895645E-5 0.0 0.0 1.6873019941146712 0.01706378545879219 86-87 4.4728140127895645E-5 0.0 0.0 1.8564414560083087 0.017712343490646675 88-89 4.4728140127895645E-5 0.0 0.0 2.0422421500995873 0.018338537452437215 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4905 0.0 29.537006 1 GTATCAA 7270 0.0 28.980755 1 GTACATG 10650 0.0 27.740807 1 TACATGG 10550 0.0 27.101343 2 ACATGGG 10320 0.0 26.420403 3 TCAACGC 8415 0.0 24.751139 4 ATCAACG 8550 0.0 24.360332 3 CAACGCA 8555 0.0 24.290762 5 AACGCAG 8705 0.0 24.034796 6 CATGGGG 5290 0.0 23.444569 4 TATCAAC 8960 0.0 23.351814 2 AGTACAT 6940 0.0 21.008623 2 CATGGGA 4870 0.0 20.509266 4 ACGCAGA 10250 0.0 20.273447 7 CGCAGAG 10355 0.0 19.976894 8 GTACCTG 2160 0.0 19.289145 1 GAGTGCA 1115 0.0 19.104439 9 GTACTAG 225 1.7217299E-6 18.938435 1 GTACTTT 4360 0.0 18.511612 14-15 TAGACTG 695 0.0 18.38974 5 >>END_MODULE