FastQCFastQC Report
Thu 26 May 2016
SRR936322_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936322_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235729
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40500.18114896751797735No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29960.13400550782317536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68350.032.2212371
GGTATCA45500.030.1079271
TACATGG106300.026.3776132
GTACATG107300.026.2434981
TCAACGC85200.025.668694
ACATGGG103600.025.368363
ATCAACG86650.025.2391513
CAACGCA87100.025.163225
AACGCAG88000.024.9597786
TATCAAC89750.024.369562
CATGGGG55600.021.8427624
ACGCAGA103600.021.0639827
AGTACAT71200.021.0566042
ATATAGG6350.020.9201872
CGCAGAG105050.020.7732378
TACCTGG20100.019.5913012
CATGGGA45850.019.5553154
TCTAGAC14200.019.3767993
GTGTAAG5750.019.0265371
GTGTAGC10850.018.8511771
Produced by FastQC (version 0.11.5)