##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936322_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2235729 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45454525123573 34.0 31.0 34.0 31.0 34.0 2 32.58299373492941 34.0 31.0 34.0 31.0 34.0 3 32.66627842641036 34.0 31.0 34.0 31.0 34.0 4 36.168485089203564 37.0 35.0 37.0 35.0 37.0 5 36.120491347564936 37.0 35.0 37.0 35.0 37.0 6 36.15415240398098 37.0 36.0 37.0 35.0 37.0 7 36.09339369843125 37.0 36.0 37.0 35.0 37.0 8 36.06838261703453 37.0 36.0 37.0 35.0 37.0 9 37.83134583842675 39.0 38.0 39.0 35.0 39.0 10-11 37.76470180419899 39.0 38.0 39.0 35.0 39.0 12-13 37.70837767010224 39.0 37.5 39.0 35.0 39.0 14-15 39.05672981832771 40.0 38.0 41.0 36.0 41.0 16-17 38.97348493489149 40.0 38.0 41.0 35.0 41.0 18-19 38.987481040859606 40.0 38.5 41.0 35.0 41.0 20-21 38.93165786193228 40.0 38.0 41.0 35.0 41.0 22-23 38.813718925683744 40.0 38.0 41.0 35.0 41.0 24-25 38.96210072866613 40.0 38.0 41.0 35.0 41.0 26-27 38.882043172495415 40.0 38.0 41.0 35.0 41.0 28-29 38.792304210394015 40.0 38.0 41.0 35.0 41.0 30-31 38.68888872488571 40.0 38.0 41.0 34.5 41.0 32-33 38.546091677479694 40.0 38.0 41.0 34.0 41.0 34-35 38.414469955884634 40.0 38.0 41.0 34.0 41.0 36-37 38.280175727916934 40.0 38.0 41.0 34.0 41.0 38-39 38.14107367216688 40.0 38.0 41.0 33.0 41.0 40-41 37.99319170615043 40.0 37.5 41.0 33.0 41.0 42-43 38.036559663537034 40.0 38.0 41.0 33.0 41.0 44-45 37.90859894021145 40.0 37.0 41.0 33.0 41.0 46-47 37.7165501722257 40.0 37.0 41.0 33.0 41.0 48-49 37.48999476233479 40.0 37.0 41.0 32.5 41.0 50-51 37.279123498420425 40.0 36.0 41.0 32.0 41.0 52-53 37.04192838219659 39.5 36.0 41.0 31.5 41.0 54-55 36.832775573425934 39.0 35.0 41.0 31.0 41.0 56-57 36.54743396896493 39.0 35.0 41.0 31.0 41.0 58-59 36.26055617653124 38.5 35.0 40.5 31.0 41.0 60-61 35.958115898662136 38.0 35.0 40.0 30.0 41.0 62-63 35.62762257858623 37.0 35.0 40.0 30.0 41.0 64-65 35.2278800337608 37.0 34.0 40.0 29.0 41.0 66-67 34.86372543362813 36.0 34.0 39.0 29.0 41.0 68-69 34.502434776307865 36.0 34.0 39.0 29.0 40.5 70-71 34.1252370032325 35.0 34.0 38.5 28.0 40.0 72-73 33.72557295629301 35.0 33.5 37.0 28.0 39.5 74-75 33.31144695980595 35.0 33.0 37.0 27.0 39.0 76-77 32.61062901630743 35.0 32.5 36.0 26.0 39.0 78-79 32.683169561248256 35.0 33.0 36.0 26.0 37.5 80-81 32.55478839340546 35.0 33.0 35.5 27.0 37.0 82-83 32.30770120171094 35.0 33.0 35.0 26.0 37.0 84-85 32.04929242318725 35.0 33.0 35.0 26.0 36.0 86-87 31.667831163794897 35.0 32.0 35.0 25.0 36.0 88-89 31.202677963205737 34.5 32.0 35.0 24.0 36.0 90-91 30.997749503629464 34.0 32.0 35.0 23.0 35.0 92-93 30.727202849719262 34.0 31.0 35.0 21.0 35.0 94-95 30.58079288679442 34.0 31.0 35.0 20.0 35.0 96-97 30.330524629774 34.0 31.0 35.0 18.5 35.0 98-99 29.880955160486806 34.0 31.0 35.0 6.0 35.0 100-101 26.49565510846798 31.0 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15570452597525275 1101 2 0.13972556180306128 1101 3 0.20149573778258656 1101 4 0.20542539963253148 1101 5 0.1766639675532531 1101 6 0.20382572273406652 1101 7 0.14921998984161178 1101 8 0.19264027409143836 1101 9 0.28043008367556155 1101 10-11 0.23479366115390832 1101 12-13 0.2680927461842515 1101 14-15 0.40338482107869567 1101 16-17 0.4813746193696318 1101 18-19 0.39220140536200887 1101 20-21 0.26053107335648207 1101 22-23 0.382085839472289 1101 24-25 0.3091177463631709 1101 26-27 0.32267624579187526 1101 28-29 0.3735191836141354 1101 30-31 0.286920661376854 1101 32-33 0.36913259203120674 1101 34-35 0.35087686841443855 1101 36-37 0.3419974031338171 1101 38-39 0.35771278260948947 1101 40-41 0.4497810044291626 1101 42-43 0.47824900819603045 1101 44-45 0.4349383593678624 1101 46-47 0.41202670017181475 1101 48-49 0.40133940520084366 1101 50-51 0.4701388514426341 1101 52-53 0.4802387585252248 1101 54-55 0.4986940464416776 1101 56-57 0.4701686013687407 1101 58-59 0.3759713704901628 1101 60-61 0.3190216191366133 1101 62-63 0.2865512821622147 1101 64-65 0.3811637530779919 1101 66-67 0.42035820329204654 1101 68-69 0.5299137097789739 1101 70-71 0.47753898559943053 1101 72-73 0.47528440376331815 1101 74-75 0.4045048126818571 1101 76-77 0.2575769193364792 1101 78-79 0.2697466931440644 1101 80-81 0.46435008608667516 1101 82-83 0.4360913918670164 1101 84-85 0.5552803136441256 1101 86-87 0.40391859654963014 1101 88-89 0.4955181790638967 1101 90-91 0.8244328714809903 1101 92-93 0.9085382005599669 1101 94-95 0.9355750556372548 1101 96-97 0.802498678160056 1101 98-99 0.612998656727914 1101 100-101 0.6799058020941864 1102 1 0.08714513863039741 1102 2 0.19192169983370633 1102 3 0.1609259275396937 1102 4 0.10661050577471087 1102 5 0.10021492065020965 1102 6 0.19770536898069224 1102 7 0.14435453361078032 1102 8 0.10411543213253793 1102 9 0.1313680906061947 1102 10-11 0.13196158622113785 1102 12-13 0.24122791511172892 1102 14-15 0.2748501167085138 1102 16-17 0.2980015399799143 1102 18-19 0.332706678787936 1102 20-21 0.22757957162921372 1102 22-23 0.2729969268704977 1102 24-25 0.1495904145496212 1102 26-27 0.21262669121716726 1102 28-29 0.22618109153262367 1102 30-31 0.22416365933430882 1102 32-33 0.2966853667101361 1102 34-35 0.24149518231936185 1102 36-37 0.20041602207287923 1102 38-39 0.26688603769024866 1102 40-41 0.22572830789501097 1102 42-43 0.2857153385706397 1102 44-45 0.24810104065030458 1102 46-47 0.33005834072734785 1102 48-49 0.29581928426505044 1102 50-51 0.17680729657690364 1102 52-53 0.1828285846126576 1102 54-55 0.12572069112002282 1102 56-57 0.3034144933780354 1102 58-59 0.19588508786470982 1102 60-61 0.14152219848590875 1102 62-63 0.25920869001872404 1102 64-65 0.25326970124373815 1102 66-67 0.2668288882176526 1102 68-69 0.2512126109015611 1102 70-71 0.20453531548900372 1102 72-73 0.021520923787250013 1102 74-75 0.06872123602181546 1102 76-77 -0.014689360378753236 1102 78-79 0.12377114976831649 1102 80-81 0.21508451236918802 1102 82-83 0.37473681931901126 1102 84-85 0.30184881428814236 1102 86-87 0.2169548988460086 1102 88-89 0.1675591862154917 1102 90-91 0.25338645886572664 1102 92-93 0.2442185722058028 1102 94-95 0.1917955155597575 1102 96-97 0.23425321729443027 1102 98-99 0.4974540414427935 1102 100-101 0.9081153971963936 1103 1 0.044514505266818105 1103 2 0.06644253866975447 1103 3 0.10760664628797656 1103 4 0.07406651238581219 1103 5 0.0864654992525189 1103 6 0.08592358769628561 1103 7 0.16761552313186456 1103 8 0.1362012509495969 1103 9 0.1539188049348752 1103 10-11 0.17269767296048855 1103 12-13 0.18243904415115964 1103 14-15 0.3024183917806269 1103 16-17 0.20138070820477338 1103 18-19 0.2242777834541485 1103 20-21 0.22723175645767668 1103 22-23 0.2591950716819085 1103 24-25 0.19418031954116088 1103 26-27 0.25035639960160694 1103 28-29 0.3128017140956416 1103 30-31 0.2773166169400838 1103 32-33 0.31394066043090163 1103 34-35 0.372682042751336 1103 36-37 0.34697670535970815 1103 38-39 0.3864009532077759 1103 40-41 0.3656633736791548 1103 42-43 0.4283504176920516 1103 44-45 0.46618416063084567 1103 46-47 0.4636151823563708 1103 48-49 0.48518058577372614 1103 50-51 0.4500440959450813 1103 52-53 0.5029444619829633 1103 54-55 0.5181034081056879 1103 56-57 0.45787816499866807 1103 58-59 0.4038915054469001 1103 60-61 0.2950229712632293 1103 62-63 0.4048458324948072 1103 64-65 0.4100022784495536 1103 66-67 0.40368775109740085 1103 68-69 0.34918220714829573 1103 70-71 0.24387007320827792 1103 72-73 0.22248604550948414 1103 74-75 0.3739619999599242 1103 76-77 0.4027425868370287 1103 78-79 0.4079062014306345 1103 80-81 0.4516730993242959 1103 82-83 0.5498920939491612 1103 84-85 0.47019044201083915 1103 86-87 0.44014423321145557 1103 88-89 0.47594140109204375 1103 90-91 0.5692261709898325 1103 92-93 0.8526296845082655 1103 94-95 0.824466343804442 1103 96-97 0.5565022738784648 1103 98-99 0.5390992856587324 1103 100-101 1.0322032976741795 1104 1 0.05391114327423452 1104 2 0.09010744284722705 1104 3 0.10845370762095996 1104 4 0.13024534282587297 1104 5 0.07635203815581093 1104 6 0.07602343017979507 1104 7 0.05020882293825224 1104 8 0.07524513816122891 1104 9 0.011217684270683037 1104 10-11 0.11278192787467844 1104 12-13 0.15870876447734616 1104 14-15 0.2622557888206316 1104 16-17 0.2740646722877358 1104 18-19 0.29906427477433084 1104 20-21 0.2952358605005969 1104 22-23 0.22365592637294895 1104 24-25 0.21980418123840195 1104 26-27 0.3322116485104729 1104 28-29 0.31628404886740213 1104 30-31 0.19611666124159655 1104 32-33 0.1875631602668193 1104 34-35 0.14669202699968054 1104 36-37 0.15824277491308436 1104 38-39 0.11431275217734793 1104 40-41 0.2572274734560054 1104 42-43 0.2385499693443336 1104 44-45 0.2466442708103429 1104 46-47 0.1737684327747786 1104 48-49 0.19855518172752795 1104 50-51 0.17849120486118153 1104 52-53 0.1873664297905293 1104 54-55 0.26173865488152614 1104 56-57 0.21094491671330218 1104 58-59 0.2706782921105031 1104 60-61 0.1397705244250389 1104 62-63 0.07899513064398889 1104 64-65 0.13124141842592252 1104 66-67 0.06833657956850203 1104 68-69 0.3165500922432969 1104 70-71 0.23191994522613157 1104 72-73 0.09995313727924326 1104 74-75 0.20782286654673499 1104 76-77 0.16109014040904412 1104 78-79 0.02612740339631614 1104 80-81 0.1000377889675832 1104 82-83 0.15898354375719492 1104 84-85 0.23211057417702108 1104 86-87 0.24916061656720956 1104 88-89 0.17682132963534514 1104 90-91 0.4828818046655421 1104 92-93 0.44023464422670244 1104 94-95 0.3206694375267496 1104 96-97 0.2677743425950361 1104 98-99 0.22811138243292461 1104 100-101 0.4897281967651814 1105 1 0.0980762870848153 1105 2 0.09952614219570677 1105 3 0.1322797842139991 1105 4 0.09123572896920962 1105 5 0.04338485239384937 1105 6 0.09946813064062354 1105 7 0.15897203205950206 1105 8 0.16428107094368727 1105 9 0.13893200971164532 1105 10-11 0.22034787416420443 1105 12-13 0.2100478018998828 1105 14-15 0.24722718561071844 1105 16-17 0.26665490151068383 1105 18-19 0.29967705882010165 1105 20-21 0.28820299660420545 1105 22-23 0.17045345934609202 1105 24-25 0.27397275147517064 1105 26-27 0.36093801113561597 1105 28-29 0.30777086954116584 1105 30-31 0.24980031768526345 1105 32-33 0.41949869379614313 1105 34-35 0.2109330487699097 1105 36-37 0.18959631257397547 1105 38-39 0.26708865397299775 1105 40-41 0.3223750084154844 1105 42-43 0.3070558985513898 1105 44-45 0.23815194799134076 1105 46-47 0.18015648298930387 1105 48-49 0.24769981663933294 1105 50-51 0.24490726238541072 1105 52-53 0.23387395799647237 1105 54-55 0.21448625141735533 1105 56-57 0.31932873603636125 1105 58-59 0.3376386051567408 1105 60-61 0.3380625254579357 1105 62-63 0.35741237003696114 1105 64-65 0.2926804677664663 1105 66-67 0.21267652077302301 1105 68-69 0.28132518957367125 1105 70-71 0.11534299464612019 1105 72-73 0.1617104966467906 1105 74-75 0.30319892923543534 1105 76-77 0.19173927436740712 1105 78-79 0.16445396664119016 1105 80-81 0.23612178110170134 1105 82-83 0.3681733181096476 1105 84-85 0.28531279291212286 1105 86-87 0.19538956142658037 1105 88-89 0.4639526163825245 1105 90-91 0.6161702799555506 1105 92-93 0.666141653999464 1105 94-95 0.46518566587571186 1105 96-97 0.44337918130471365 1105 98-99 0.4984588915700634 1105 100-101 0.6641869207442035 1106 1 -0.025467375716651475 1106 2 0.013263666600607849 1106 3 0.04593037729464555 1106 4 -0.04852133835743899 1106 5 -0.033298551323767356 1106 6 0.012224708681678464 1106 7 0.10753386493284012 1106 8 0.07756018699937783 1106 9 -0.039289555907252804 1106 10-11 0.08603565295011606 1106 12-13 0.016583089842079346 1106 14-15 0.09914181239981446 1106 16-17 -0.03524903700084536 1106 18-19 0.12700603450307568 1106 20-21 0.08741538930576809 1106 22-23 0.04532911905033643 1106 24-25 0.05918825090276414 1106 26-27 -0.025485026140344758 1106 28-29 -0.011232175430357927 1106 30-31 -0.0011811037131437274 1106 32-33 0.09147307993272591 1106 34-35 0.07775188484023232 1106 36-37 0.09375167737676549 1106 38-39 0.166806958479647 1106 40-41 0.009674484452368404 1106 42-43 -0.01952062239050889 1106 44-45 -0.04582299868602746 1106 46-47 -0.03847532247454666 1106 48-49 -0.08183426946543193 1106 50-51 -0.028772497817151077 1106 52-53 -0.11983321170011862 1106 54-55 -0.0049274609302187855 1106 56-57 -0.0016172002805134866 1106 58-59 -0.11648944515891912 1106 60-61 -0.07630061946085931 1106 62-63 -0.10717285353805295 1106 64-65 -0.02002148130032566 1106 66-67 -0.08619669725359103 1106 68-69 -0.05050568862272797 1106 70-71 -0.14945409434211143 1106 72-73 -0.1893421799354229 1106 74-75 -0.08877678699791858 1106 76-77 -0.03801475273806432 1106 78-79 -0.1399215766795905 1106 80-81 -0.08080845057265407 1106 82-83 0.025120936048857345 1106 84-85 0.14661148989232942 1106 86-87 0.1542382676699603 1106 88-89 0.19015290995388412 1106 90-91 0.18309148597328928 1106 92-93 0.27348283807060625 1106 94-95 0.12065806399267487 1106 96-97 0.07939653906748845 1106 98-99 7.675595256557699E-4 1106 100-101 0.2896171832958032 1107 1 0.08347072833920777 1107 2 0.17415920252794592 1107 3 0.15155104253751261 1107 4 0.07637882788425543 1107 5 0.06256897199049405 1107 6 0.14143424223863832 1107 7 0.1388535636863253 1107 8 0.10255461602916682 1107 9 0.08340046347775143 1107 10-11 0.08330686355645156 1107 12-13 0.10662697725523174 1107 14-15 0.23901876790896637 1107 16-17 0.27071012630845104 1107 18-19 0.27313702033802656 1107 20-21 0.13954709887384098 1107 22-23 0.06574746440315948 1107 24-25 0.16965812526505886 1107 26-27 0.22874176263410106 1107 28-29 0.22279121870050744 1107 30-31 0.221874874184401 1107 32-33 0.26614838604028535 1107 34-35 0.35568709593562886 1107 36-37 0.30972611401856653 1107 38-39 0.27072354774434615 1107 40-41 0.18619757784943403 1107 42-43 0.15715185188935976 1107 44-45 0.27022504378350476 1107 46-47 0.31011482765570264 1107 48-49 0.3029676800783392 1107 50-51 0.330486306780827 1107 52-53 0.4599448073220316 1107 54-55 0.4718149709313977 1107 56-57 0.46018319925172335 1107 58-59 0.4272086536119204 1107 60-61 0.5281861314901519 1107 62-63 0.40680214710285156 1107 64-65 0.522721417931173 1107 66-67 0.41684860212936314 1107 68-69 0.43099376019632984 1107 70-71 0.6714175209237325 1107 72-73 0.6000721878432671 1107 74-75 0.577586187841014 1107 76-77 0.5297984984801616 1107 78-79 0.4211273827814921 1107 80-81 0.6810370097275324 1107 82-83 0.7925819836278976 1107 84-85 0.6369850505606891 1107 86-87 0.5989466347742152 1107 88-89 0.8164191964141558 1107 90-91 0.613337515794246 1107 92-93 0.7457766283615399 1107 94-95 0.8731578520000021 1107 96-97 0.7283485064580866 1107 98-99 0.8967168725707957 1107 100-101 0.8968563879387368 1108 1 -0.13131540838884348 1108 2 -0.11300095590731019 1108 3 -0.11701149616855133 1108 4 -0.05745387211967312 1108 5 -0.1201605935474035 1108 6 -0.034266021757758836 1108 7 -0.031064879951102853 1108 8 -0.2060302248526682 1108 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-0.6893456819860475 2316 80-81 -0.8575371461779184 2316 82-83 -1.0412293481895674 2316 84-85 -0.8186461791502957 2316 86-87 -0.9251020159615493 2316 88-89 -0.7419699093675867 2316 90-91 -0.8155962293971655 2316 92-93 -0.912646798926918 2316 94-95 -0.8209213305761125 2316 96-97 -0.7651872522617218 2316 98-99 -0.9318797353202903 2316 100-101 -0.9864236755560469 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 21.0 5 44.0 6 73.0 7 160.0 8 389.0 9 645.0 10 1173.0 11 1692.0 12 2631.0 13 3704.0 14 4614.0 15 6090.0 16 7800.0 17 10656.0 18 9787.0 19 6830.0 20 6595.0 21 7157.0 22 7871.0 23 9043.0 24 10965.0 25 13329.0 26 16977.0 27 21221.0 28 26779.0 29 33179.0 30 41588.0 31 52484.0 32 65777.0 33 85371.0 34 119527.0 35 181774.0 36 317926.0 37 560223.0 38 526305.0 39 75298.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.157738864014696 17.687166350955742 12.753958943229435 24.401135841800127 2 16.784323935962238 19.01855942765778 38.03380834659774 26.16330828978225 3 20.188314415566467 22.81112782452614 27.950927862902887 29.049629897004515 4 13.480614153146467 14.635450003108605 34.155660189584694 37.72827565416023 5 14.800407383900286 36.78120201509217 33.05503484545756 15.363355755549978 6 36.00575024969484 33.37224681524461 15.877639910740523 14.744363024320032 7 31.62623913721207 30.776359746641923 18.96942786894118 18.627973247204828 8 28.415742695111973 34.13486160442522 18.247560415417073 19.20183528504573 9 27.349558018883325 15.262225430720807 18.578325011662862 38.809891538733005 10-11 26.93092844602981 25.14271656845095 25.688266669648545 22.23808831587069 12-13 26.94787267231807 21.880036811284022 26.799598788313872 24.372491728084036 14-15 23.812680338269978 23.60632706378993 24.103413249101298 28.477579348838788 16-17 24.12787330037442 26.82300903559625 24.877075009180516 24.17204265484881 18-19 24.121505781783036 25.654160231405505 26.516652957491715 23.707681029319744 20-21 24.727376090053948 24.904835184446757 26.466631763768962 23.901156961730337 22-23 24.357105237556905 24.577570388162243 26.54985905035386 24.515465323927 24-25 24.37523077373808 24.9334040946278 26.60360770779253 24.08775742384159 26-27 24.089708523338484 25.30671807522079 26.50297336163189 24.10060003980883 28-29 23.965080901487777 25.181510608396007 26.780843608348594 24.072564881767622 30-31 24.053046271461596 25.274806621704037 26.518081404619608 24.154065702214755 32-33 23.890511420678102 25.1194534516764 26.582236866382324 24.407798261263178 34-35 24.138489373550506 25.18500958314976 26.519963590740243 24.156537452559494 36-37 24.456541429253214 25.07086061938689 26.17739106787188 24.29520688348801 38-39 24.261683112473033 25.04755723753392 26.453006113894943 24.237753536098104 40-41 24.120293400282772 25.06551589490083 26.77769592484201 24.036494779974387 42-43 24.311145374084802 25.05430748424563 26.141687236751558 24.492859904918014 44-45 24.010483749584292 25.265420511837604 26.277072377815763 24.447023360762337 46-47 24.277541719406084 25.43547904764184 26.047729742750924 24.239249490201146 48-49 24.562972724881497 25.442328235464633 25.71768740348673 24.277011636167135 50-51 24.439939076894174 25.392790765810595 25.713017281675434 24.454252875619797 52-53 24.515039620324792 25.29988660736851 25.70425323012442 24.480820542182283 54-55 24.541272657374673 25.332537766857126 25.66325844855144 24.462931127216763 56-57 24.22349427297603 25.707029987621173 25.576666528751417 24.49280921065138 58-59 24.35350857280877 25.317366688993726 25.839126627983823 24.48999811021368 60-61 24.421681045433562 25.4514461663977 25.80050847518276 24.326364312985977 62-63 24.326405391708924 25.460397928371464 25.928455550739827 24.28474112917979 64-65 24.407089652544226 25.465098543683457 25.75382127101515 24.37399053275717 66-67 24.309453426600452 25.737578212744033 25.61104230432221 24.341926056333303 68-69 24.194658654962208 25.38006618870176 25.98848071479146 24.43679444154457 70-71 24.32516872039971 25.282643150959277 25.812896082278435 24.579292046362582 72-73 24.266196895511627 25.63458355408835 25.375738275979522 24.7234812744205 74-75 24.15858540994906 25.82218148979595 25.480234858518187 24.538998241736813 76-77 24.299039454105024 25.707962589147215 25.592636135965563 24.400361820782198 78-79 24.069723553123442 25.71148927063031 25.74679341234118 24.471993763905065 80-81 24.158905686733505 25.91065854662749 25.49790256176635 24.43253320487265 82-83 24.394235616212878 25.79064815100578 25.393730635510835 24.421385597270508 84-85 23.99938006381854 25.775278054348632 25.287447321079533 24.9378945607533 86-87 24.258790042981804 25.534518445275 25.666289601878695 24.5404019098645 88-89 24.20205611025185 25.542797090926108 25.46028995365384 24.794856845168205 90-91 24.06180772798498 25.824445144856256 25.563503110912706 24.550244016246065 92-93 24.03060559632758 25.733229173465432 25.482787940691676 24.753377289515313 94-95 24.08105884349961 25.43449221032753 25.766202699889007 24.718246246283858 96-97 24.20470869082239 25.286440400872312 25.609452538819937 24.89939836948536 98-99 23.816178883007737 25.508129876746317 25.529353906104806 25.146337334141144 100-101 23.95712869420492 25.41722120601714 25.634780290596137 24.990869809181806 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 180.0 1 131.5 2 161.0 3 272.5 4 227.5 5 193.0 6 442.0 7 894.0 8 1296.5 9 1333.0 10 1066.5 11 910.5 12 963.0 13 1024.5 14 1078.5 15 1169.5 16 1222.5 17 1277.5 18 1345.0 19 1411.0 20 1560.0 21 1764.0 22 1961.0 23 2300.0 24 2746.0 25 3284.5 26 4189.5 27 5304.5 28 6287.5 29 8111.5 30 11233.5 31 14868.0 32 19514.0 33 23010.5 34 25619.0 35 29687.5 36 35722.0 37 41018.5 38 44750.0 39 47888.5 40 49820.0 41 53824.5 42 56340.0 43 60733.5 44 66868.5 45 70501.0 46 74329.0 47 77679.0 48 85114.0 49 88806.0 50 87405.5 51 88900.0 52 92061.5 53 97251.0 54 101629.0 55 98271.5 56 91665.0 57 85018.5 58 79947.0 59 75106.0 60 66215.5 61 56405.0 62 46030.5 63 35421.0 64 26752.0 65 20113.0 66 14197.5 67 11426.0 68 8713.0 69 5973.0 70 4531.0 71 2951.5 72 2181.0 73 1927.5 74 1534.5 75 1172.5 76 776.0 77 387.0 78 212.5 79 103.5 80 43.0 81 25.5 82 13.5 83 6.0 84 5.5 85 4.0 86 3.0 87 3.0 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26125706648703845 2 0.01892000327409986 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.7891256051158258E-4 12-13 6.709221019184346E-5 14-15 0.0 16-17 7.156502420463303E-4 18-19 0.0 20-21 1.1182035031973912E-4 22-23 6.709221019184346E-5 24-25 0.0019456740955634605 26-27 0.0019680381656274086 28-29 0.002102222586011095 30-31 9.169268726218606E-4 32-33 0.0011405675732613389 34-35 0.001520756764348452 36-37 8.945628025579129E-5 38-39 8.945628025579129E-5 40-41 5.367376815347477E-4 42-43 0.003555887140167703 44-45 0.0035111590000398082 46-47 0.012904068426897893 48-49 0.012747519936450258 50-51 0.00554628937585906 52-53 0.005993570777138016 54-55 2.4600477070342607E-4 56-57 0.002527139917226104 58-59 0.0011852957133892345 60-61 0.0011182035031973912 62-63 0.0 64-65 8.945628025579129E-4 66-67 0.0 68-69 0.0 70-71 1.5654849044763477E-4 72-73 0.0013642082739008172 74-75 0.0 76-77 0.18953549379195778 78-79 0.10198015949160207 80-81 0.0011405675732613389 82-83 0.0 84-85 6.709221019184346E-4 86-87 0.12036342508416717 88-89 0.5765904543887027 90-91 0.30779669629011386 92-93 0.2891003337166535 94-95 0.0019904022356913562 96-97 0.002437683636970313 98-99 0.002482411777098208 100-101 6.485580318544869E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2235729.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.83384518700463 #Duplication Level Percentage of deduplicated Percentage of total 1 61.66758105515243 20.86451390477087 2 15.515144304045563 10.498739808742286 3 7.232042666181671 7.3406343586020855 4 4.127057703937594 5.585369257314374 5 2.6427838356105573 4.470776957838296 6 1.7599529872253643 3.572758614371359 7 1.224217867144434 2.899399845449085 8 0.8926397784284094 2.4161148856887134 9 0.7222368779760096 2.1992445646007277 >10 3.796893090105001 23.073543360265685 >50 0.2670994024918016 6.2195376214217575 >100 0.14468881137350392 8.66768886142874 >500 0.006604845110004843 1.4808731496038054 >1k 0.0010567752176007738 0.7108048099021822 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4050 0.18114896751797735 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2996 0.13400550782317536 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.025532611510608E-4 2 0.0 0.0 0.0 0.0 4.025532611510608E-4 3 0.0 0.0 0.0 0.0 4.025532611510608E-4 4 0.0 0.0 0.0 0.0 4.025532611510608E-4 5 0.0 0.0 0.0 0.0 4.4728140127895646E-4 6 0.0 0.0 0.0 0.0 6.709221019184346E-4 7 0.0 0.0 0.0 0.0 6.709221019184346E-4 8 0.0 0.0 0.0 0.0 6.709221019184346E-4 9 0.0 0.0 0.0 0.0 6.709221019184346E-4 10-11 0.0 0.0 0.0 6.709221019184346E-5 8.051065223021216E-4 12-13 0.0 0.0 0.0 8.945628025579129E-5 0.0024600477070342605 14-15 0.0 0.0 0.0 1.7891256051158258E-4 0.0032204260892084863 16-17 0.0 0.0 0.0 6.932861719823825E-4 0.003667707490487443 18-19 0.0 0.0 0.0 8.945628025579129E-4 0.004025532611510608 20-21 0.0 0.0 0.0 0.0012971160637089735 0.004741182853556939 22-23 0.0 0.0 0.0 0.002392955496842417 0.005076643904516155 24-25 0.0 0.0 0.0 0.003645343420423495 0.005322648675219581 26-27 0.0 0.0 0.0 0.003980804471382712 0.005814658216626434 28-29 0.0 0.0 0.0 0.0045622702930453556 0.005948842637010121 30-31 0.0 0.0 0.0 0.007603783821742259 0.005993570777138016 32-33 0.0 0.0 0.0 0.012501515165746834 0.006217211477777495 34-35 0.0 0.0 0.0 0.019344920605314866 0.006597400668864608 36-37 0.0 0.0 0.0 0.0297889413251785 0.006709221019184346 38-39 0.0 0.0 0.0 0.04761310516614491 0.0070223180000796165 40-41 0.0 0.0 0.0 0.06834459811542454 0.007111774280335407 42-43 2.2364070063947822E-5 0.0 0.0 0.08798025163157074 0.007313050910910938 44-45 4.4728140127895645E-5 0.0 0.0 0.11202162695031465 0.007872152662509634 46-47 4.4728140127895645E-5 0.0 0.0 0.13966361754935414 0.008364162203916486 48-49 4.4728140127895645E-5 0.0 0.0 0.17256116461342141 0.008789079535131493 50-51 4.4728140127895645E-5 0.0 0.0 0.20908169102784818 0.009213996866346502 52-53 4.4728140127895645E-5 0.0 0.0 0.24341053857600808 0.010175651879096258 54-55 8.945628025579129E-5 0.0 0.0 0.281608370245231 0.010399292579735736 56-57 8.945628025579129E-5 0.0 0.0 0.327901995277603 0.010712389560631006 58-59 8.945628025579129E-5 0.0 0.0 0.3821348651826764 0.010958394331334432 60-61 8.945628025579129E-5 0.0 0.0 0.4409523694508592 0.011137306891846015 62-63 8.945628025579129E-5 0.0 0.0 0.501089353852815 0.011987141554276033 64-65 8.945628025579129E-5 0.0 0.0 0.5628812794395027 0.012300238535171301 66-67 8.945628025579129E-5 0.0 0.0 0.6365485262301469 0.01243442295555499 68-69 8.945628025579129E-5 0.0 0.0 0.7220240020145554 0.012479151095682884 70-71 8.945628025579129E-5 0.0 0.0 0.8167358387353745 0.012590971446002623 72-73 8.945628025579129E-5 0.0 0.0 0.9126106070995188 0.013552626458752381 74-75 8.945628025579129E-5 0.0 0.0 1.0120188985337668 0.014290640770862659 76-77 8.945628025579129E-5 0.0 0.0 1.1218264825477506 0.01458137368169398 78-79 8.945628025579129E-5 0.0 0.0 1.2482952987593756 0.014760286242205562 80-81 8.945628025579129E-5 0.0 0.0 1.3928790117227983 0.015006291012908988 82-83 8.945628025579129E-5 0.0 0.0 1.5438141205843823 0.01623631486642612 84-85 8.945628025579129E-5 0.0 0.0 1.6979920196052385 0.01643759149700165 86-87 8.945628025579129E-5 0.0 0.0 1.8672209377791316 0.017242698019303768 88-89 8.945628025579129E-5 0.0 0.0 2.0547436652653337 0.0179583482613501 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6835 0.0 32.221237 1 GGTATCA 4550 0.0 30.107927 1 TACATGG 10630 0.0 26.377613 2 GTACATG 10730 0.0 26.243498 1 TCAACGC 8520 0.0 25.66869 4 ACATGGG 10360 0.0 25.36836 3 ATCAACG 8665 0.0 25.239151 3 CAACGCA 8710 0.0 25.16322 5 AACGCAG 8800 0.0 24.959778 6 TATCAAC 8975 0.0 24.36956 2 CATGGGG 5560 0.0 21.842762 4 ACGCAGA 10360 0.0 21.063982 7 AGTACAT 7120 0.0 21.056604 2 ATATAGG 635 0.0 20.920187 2 CGCAGAG 10505 0.0 20.773237 8 TACCTGG 2010 0.0 19.591301 2 CATGGGA 4585 0.0 19.555315 4 TCTAGAC 1420 0.0 19.376799 3 GTGTAAG 575 0.0 19.026537 1 GTGTAGC 1085 0.0 18.851177 1 >>END_MODULE