FastQCFastQC Report
Thu 26 May 2016
SRR936321_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936321_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1599829
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA60380.3774153362640632No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59920.3745400289655957No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA54600.34128647499201475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43130.26959131257153107No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT30580.19114542866768885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29810.18633241427677583No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG24640.1540164605092169No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA22060.13788973696563822No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT20000.1250133608029358No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC19030.11895021280399341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG119600.028.4184342
GTACATG122500.028.3332881
TCATACG700.00853384627.0500952
ACATGGG122550.026.7678343
TACCTGG12100.026.6029872
GTGTACG2800.025.36661
TAAGGTG4950.024.8618985
GTATAGG4450.024.4735471
CATGGGG43800.024.4230824
ATGGGAT28650.024.286165
CATGGGA79150.023.860974
TATTCCG2002.1334927E-823.6666135
GTACGAT1050.002241136722.5410423
GTACCTG15900.022.0375691
GAGTACT56850.021.65331612-13
GTCTACG1100.002930797421.5231781
TGGGATA8000.021.2979566
AGTACAT85600.021.0696852
AGTACTT58750.021.03362712-13
GTACTTT58950.020.68084114-15
Produced by FastQC (version 0.11.5)