##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936321_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1599829 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.611268454316054 33.0 31.0 34.0 30.0 34.0 2 31.688193550685728 33.0 31.0 34.0 30.0 34.0 3 31.756247698972828 34.0 31.0 34.0 30.0 34.0 4 35.26373068621709 37.0 35.0 37.0 33.0 37.0 5 35.26213551573324 37.0 35.0 37.0 33.0 37.0 6 35.361877425649865 37.0 35.0 37.0 33.0 37.0 7 35.30840483576682 37.0 35.0 37.0 33.0 37.0 8 35.31316846988022 37.0 35.0 37.0 33.0 37.0 9 36.95197049184632 39.0 37.0 39.0 33.0 39.0 10-11 36.86736176178829 39.0 37.0 39.0 33.0 39.0 12-13 36.776035126254115 39.0 37.0 39.0 33.0 39.0 14-15 37.94802382004577 40.0 38.0 41.0 33.0 41.0 16-17 37.902103287288824 40.0 38.0 41.0 33.0 41.0 18-19 37.90850459642874 40.0 38.0 41.0 33.0 41.0 20-21 37.83867775868546 40.0 38.0 41.0 32.5 41.0 22-23 37.681606909238425 40.0 38.0 41.0 32.0 41.0 24-25 37.82460844252729 40.0 38.0 41.0 33.0 41.0 26-27 37.754720348237214 40.0 38.0 41.0 32.5 41.0 28-29 37.61776883654441 40.0 38.0 41.0 32.0 41.0 30-31 37.458555570626615 40.0 37.0 41.0 32.0 41.0 32-33 37.25303485560019 40.0 37.0 41.0 31.5 41.0 34-35 36.981573030617646 40.0 36.5 41.0 31.0 41.0 36-37 36.75889423182103 40.0 36.0 41.0 30.0 41.0 38-39 36.575883422540784 40.0 36.0 41.0 30.0 41.0 40-41 36.376128636248 40.0 35.5 41.0 30.0 41.0 42-43 36.17618914271463 39.5 35.0 41.0 29.5 41.0 44-45 35.95880559734822 39.0 35.0 41.0 28.5 41.0 46-47 35.62928819267559 39.0 35.0 41.0 27.5 41.0 48-49 35.398033164794484 39.0 35.0 41.0 26.5 41.0 50-51 35.07723137910364 38.5 34.5 40.5 26.0 41.0 52-53 35.07953037480881 39.0 35.0 40.0 26.0 41.0 54-55 35.16059560115487 39.0 35.0 41.0 26.0 41.0 56-57 35.02546459652875 39.0 35.0 41.0 26.0 41.0 58-59 34.800310220654836 38.5 35.0 41.0 26.0 41.0 60-61 34.50627879604633 38.0 34.0 40.0 25.0 41.0 62-63 34.19833432198067 37.5 34.0 40.0 23.5 41.0 64-65 33.84796968926054 37.0 34.0 40.0 23.0 41.0 66-67 33.49900958164904 36.5 33.5 39.5 22.0 41.0 68-69 33.13311828951719 36.0 33.0 39.0 22.0 41.0 70-71 32.71333530021021 35.5 33.0 39.0 20.5 40.5 72-73 32.29332009858554 35.0 32.5 38.0 19.0 40.0 74-75 31.874906943179553 35.0 32.0 37.0 18.0 39.5 76-77 31.46287290704194 35.0 32.0 37.0 15.0 39.0 78-79 31.067925697058875 35.0 31.5 36.0 10.0 39.0 80-81 30.688872060701488 35.0 31.0 36.0 7.0 37.5 82-83 30.3484309885619 35.0 31.0 35.0 4.5 37.0 84-85 30.032190627873355 34.0 31.0 35.0 2.0 36.5 86-87 29.697371406569076 34.0 30.5 35.0 2.0 36.0 88-89 29.349387028238645 34.0 30.0 35.0 2.0 36.0 90-91 28.982409995068224 34.0 29.5 35.0 2.0 35.5 92-93 28.698357136919007 34.0 29.0 35.0 2.0 35.0 94-95 28.341084265880916 34.0 29.0 35.0 2.0 35.0 96-97 27.897980346649547 34.0 29.0 35.0 2.0 35.0 98-99 27.383076566308024 34.0 28.0 35.0 2.0 35.0 100-101 24.168514259961533 30.0 14.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3383770473365466 1101 2 0.10805809262696542 1101 3 0.2712743295603275 1101 4 -0.041586216549958976 1101 5 0.3216052307287143 1101 6 0.16799480555619795 1101 7 0.3080992233284263 1101 8 0.22464609095071353 1101 9 0.3011901221360418 1101 10-11 0.32268688804891354 1101 12-13 0.3301836144787984 1101 14-15 0.3165807571521455 1101 16-17 0.32527451962241827 1101 18-19 0.3820168291620263 1101 20-21 0.5251390504174509 1101 22-23 0.5348940687598613 1101 24-25 0.4809490463343593 1101 26-27 0.24957830582876284 1101 28-29 0.4209275506790533 1101 30-31 0.4441438861206706 1101 32-33 0.6057871248408375 1101 34-35 0.519756680551211 1101 36-37 0.4709217762639142 1101 38-39 0.48852436931684196 1101 40-41 0.45712070606620614 1101 42-43 0.42215285855828455 1101 44-45 0.4102385777866431 1101 46-47 0.4232856455158682 1101 48-49 0.49018673681634795 1101 50-51 0.5623117087658827 1101 52-53 0.6130425937015502 1101 54-55 0.6281079048154226 1101 56-57 0.6124532989209328 1101 58-59 0.6409725548132954 1101 60-61 0.539571286988263 1101 62-63 0.4071445514215384 1101 64-65 0.47873621014500145 1101 66-67 0.6750016738438376 1101 68-69 0.7109366364710326 1101 70-71 0.6529970523280255 1101 72-73 0.6945827982409298 1101 74-75 0.6126147355110483 1101 76-77 0.6106611976607326 1101 78-79 0.570797631422927 1101 80-81 0.5751072690625456 1101 82-83 0.734776751678897 1101 84-85 0.6619754115693652 1101 86-87 0.5324287454690335 1101 88-89 0.548068533285619 1101 90-91 0.6674635543122704 1101 92-93 0.7066752508802843 1101 94-95 1.0715918024808637 1101 96-97 1.0194532134540673 1101 98-99 1.132856476189506 1101 100-101 1.222798469332215 1102 1 0.16232587305867696 1102 2 0.2527752483843244 1102 3 0.21642458497152006 1102 4 0.07795235590858596 1102 5 0.16989763179842754 1102 6 0.11355259393562278 1102 7 0.02693726860191248 1102 8 0.12207101437849133 1102 9 0.15328582335352792 1102 10-11 0.19110691801306956 1102 12-13 0.1539861987593838 1102 14-15 0.2109121116240118 1102 16-17 0.2988820300919812 1102 18-19 0.21310591883461427 1102 20-21 0.21814323824055037 1102 22-23 0.19406253787786198 1102 24-25 0.28843982954420255 1102 26-27 0.17047804862790628 1102 28-29 -0.04105376518712944 1102 30-31 0.09377751551622282 1102 32-33 -0.21784007197661737 1102 34-35 -0.15539582472830915 1102 36-37 -0.1855949162251065 1102 38-39 -0.10758003480041367 1102 40-41 -0.32925071651531823 1102 42-43 -0.1622652692444646 1102 44-45 -0.34227667274131335 1102 46-47 -0.2507859002970676 1102 48-49 -0.3725086565782405 1102 50-51 -0.4739387833928177 1102 52-53 -0.31433319055653186 1102 54-55 -0.34675902058609864 1102 56-57 -0.18508002725373274 1102 58-59 -0.18882874090632384 1102 60-61 -0.1100615754067178 1102 62-63 -0.2276775300134659 1102 64-65 -0.42904117561026567 1102 66-67 -0.3328940856656857 1102 68-69 -0.18545615985963337 1102 70-71 -0.27929776223680136 1102 72-73 -0.37814624091188875 1102 74-75 -0.463227558945583 1102 76-77 -0.4465677826887138 1102 78-79 -0.5268044691359606 1102 80-81 -0.6315334218515645 1102 82-83 -0.39173053226982546 1102 84-85 -0.41637241807469394 1102 86-87 -0.4902593869868497 1102 88-89 -0.49936329314726535 1102 90-91 -0.5144204036151905 1102 92-93 -0.4723991839681325 1102 94-95 -0.583604370697639 1102 96-97 -0.434536212164776 1102 98-99 -0.3094101127544384 1102 100-101 -0.19147464422989202 1103 1 0.40269222111892233 1103 2 0.38605926183878836 1103 3 0.3107843636239025 1103 4 0.32746556870413457 1103 5 0.27845068519212646 1103 6 0.2677728959705661 1103 7 0.36217928124752774 1103 8 0.2432657391242472 1103 9 0.36605079440276 1103 10-11 0.36867722350040566 1103 12-13 0.43850567517954886 1103 14-15 0.42734352246846896 1103 16-17 0.5276424242564488 1103 18-19 0.507043026125082 1103 20-21 0.5668767037511557 1103 22-23 0.5528478879010379 1103 24-25 0.6754420686667046 1103 26-27 0.6546517926971873 1103 28-29 0.6487325810976117 1103 30-31 0.6663019041963167 1103 32-33 0.4615792357695554 1103 34-35 0.4228462175092389 1103 36-37 0.5651917358428804 1103 38-39 0.4424347611632484 1103 40-41 0.4117839159036265 1103 42-43 0.3384010423190844 1103 44-45 0.5278423854462844 1103 46-47 0.5706223795241883 1103 48-49 0.6572386283624212 1103 50-51 0.6482319817557851 1103 52-53 0.7183037460871518 1103 54-55 0.6492519876108602 1103 56-57 0.6547033598382725 1103 58-59 0.5761176483853419 1103 60-61 0.5738399229311995 1103 62-63 0.5317573628144316 1103 64-65 0.515176561790966 1103 66-67 0.5837582782361039 1103 68-69 0.46678394903866405 1103 70-71 0.3859800566408822 1103 72-73 0.571466598038981 1103 74-75 0.595643114468043 1103 76-77 0.5606570863670015 1103 78-79 0.4966890551639409 1103 80-81 0.4950282921128668 1103 82-83 0.5252424108446263 1103 84-85 0.5951708995749172 1103 86-87 0.7088360864346654 1103 88-89 0.6423322256485413 1103 90-91 0.7012430967152525 1103 92-93 0.729828101761651 1103 94-95 0.5768479912313644 1103 96-97 0.6229973993326716 1103 98-99 0.7739676084698779 1103 100-101 1.149360735878414 1104 1 0.06371854947650846 1104 2 0.041952232522884714 1104 3 0.10587378509401546 1104 4 0.1488228151272324 1104 5 0.19640466323451022 1104 6 0.21111626087937196 1104 7 0.21534475648586948 1104 8 0.1104099621664929 1104 9 0.08692053866907656 1104 10-11 0.15245419269358962 1104 12-13 0.17334415791726343 1104 14-15 0.22883510366655457 1104 16-17 0.3276178298965675 1104 18-19 0.1814525470021664 1104 20-21 0.27740490099411375 1104 22-23 0.35967825640653217 1104 24-25 0.33017467173185366 1104 26-27 0.31478800541361807 1104 28-29 0.35234742770220606 1104 30-31 0.38756272738289255 1104 32-33 0.49352640365275846 1104 34-35 0.5724287806800348 1104 36-37 0.5195830206693941 1104 38-39 0.4940219076550747 1104 40-41 0.5190622735342174 1104 42-43 0.5851999624550999 1104 44-45 0.5370273779297534 1104 46-47 0.6856081615056766 1104 48-49 0.6819697824575286 1104 50-51 0.478986323184607 1104 52-53 0.43095868817124483 1104 54-55 0.6871420775137338 1104 56-57 0.8175347044919974 1104 58-59 0.703554801693052 1104 60-61 0.6273703098348022 1104 62-63 0.6182737433254033 1104 64-65 0.498476943766164 1104 66-67 0.6549439361018727 1104 68-69 0.6563788189221 1104 70-71 0.5494696340659218 1104 72-73 0.5969741903560077 1104 74-75 0.6910034552047897 1104 76-77 0.7222508485509849 1104 78-79 0.685496047588309 1104 80-81 0.8052332355792053 1104 82-83 0.7496761924371782 1104 84-85 0.6031092535258864 1104 86-87 0.6173648480133167 1104 88-89 0.7408366883809379 1104 90-91 0.8526750032261816 1104 92-93 0.72030469851234 1104 94-95 0.4827276161387779 1104 96-97 0.5759389991671853 1104 98-99 0.5284548875615265 1104 100-101 0.7731156464092805 1105 1 0.13620810159989105 1105 2 0.16751501851897643 1105 3 0.10447168212368396 1105 4 0.060759609098646195 1105 5 0.08647260953111413 1105 6 0.04976900839818654 1105 7 0.15802148429239082 1105 8 0.2008382387996548 1105 9 0.15044418085939526 1105 10-11 0.20265210279604418 1105 12-13 0.0578761910908554 1105 14-15 0.08793986598340808 1105 16-17 0.19499251406665508 1105 18-19 0.1426449973727344 1105 20-21 0.21488887646697918 1105 22-23 0.2723925132528322 1105 24-25 0.24009070666198085 1105 26-27 0.18140594063977034 1105 28-29 0.2528061722262507 1105 30-31 0.22784197879063584 1105 32-33 0.3658664472256632 1105 34-35 0.17441451016254206 1105 36-37 0.1644210400309376 1105 38-39 0.23630562314509262 1105 40-41 0.1794923591840174 1105 42-43 0.2432608347141283 1105 44-45 0.23812880668464942 1105 46-47 0.31632825599802317 1105 48-49 0.2557617805279122 1105 50-51 0.23766879749587844 1105 52-53 0.1293159813852185 1105 54-55 0.3079671271926472 1105 56-57 0.3626287020090899 1105 58-59 0.39448315865023886 1105 60-61 0.1789645690378805 1105 62-63 0.010829202573972907 1105 64-65 0.0840434306512492 1105 66-67 0.21568937984260117 1105 68-69 0.16850337316981978 1105 70-71 0.2685505247281341 1105 72-73 0.22340604083272098 1105 74-75 0.12180856887593094 1105 76-77 0.27230537222033746 1105 78-79 0.2533790516238419 1105 80-81 0.2035108031745132 1105 82-83 0.1987654929553493 1105 84-85 0.12763707946994884 1105 86-87 0.25563056724012867 1105 88-89 0.38460471813166563 1105 90-91 0.466758358846171 1105 92-93 0.33366490778748314 1105 94-95 0.4202263215180899 1105 96-97 0.42697039182000296 1105 98-99 0.6132699190628124 1105 100-101 0.4852518464892839 1106 1 0.11666783255076396 1106 2 0.11722051346589879 1106 3 0.08817415176980248 1106 4 0.05912725577507416 1106 5 0.20081355752445518 1106 6 0.16771180385144646 1106 7 0.16890907024760082 1106 8 0.16404281602417825 1106 9 0.14403850541661 1106 10-11 0.18310712632865034 1106 12-13 0.10855020372108015 1106 14-15 0.23008282624113718 1106 16-17 0.10911127191796055 1106 18-19 0.17953681014122935 1106 20-21 0.0908813571508702 1106 22-23 0.06351069097087247 1106 24-25 0.25889594597367704 1106 26-27 0.0794824895372912 1106 28-29 0.036438209928633114 1106 30-31 0.08694760518660871 1106 32-33 0.20273109146589263 1106 34-35 0.010421955953255235 1106 36-37 0.060913430601395646 1106 38-39 0.09338011064173202 1106 40-41 0.010714153902924295 1106 42-43 0.023656858604496733 1106 44-45 0.125593489839531 1106 46-47 0.19991716478261168 1106 48-49 0.35732970947010756 1106 50-51 0.24816634022354123 1106 52-53 0.10579861639173771 1106 54-55 0.03148857194862131 1106 56-57 0.10834808966811948 1106 58-59 0.28021035614032996 1106 60-61 0.2466433455753929 1106 62-63 0.23350712280968366 1106 64-65 0.23067390549077516 1106 66-67 0.2157149343988891 1106 68-69 0.38566419920066863 1106 70-71 0.44788410673270107 1106 72-73 0.3565430374640073 1106 74-75 0.3941472860013455 1106 76-77 0.20201756515096037 1106 78-79 0.18359426582033933 1106 80-81 0.375991505001263 1106 82-83 0.3979753640079515 1106 84-85 0.3902444422307738 1106 86-87 0.3638875133720205 1106 88-89 0.33328780666430546 1106 90-91 0.5559334488026906 1106 92-93 0.49051922260706604 1106 94-95 0.562645001988404 1106 96-97 0.6503992573501343 1106 98-99 0.5796073952231033 1106 100-101 0.4258406413991729 1107 1 0.07272776431162953 1107 2 0.18932962190678637 1107 3 0.2869771854443286 1107 4 0.15989680035303167 1107 5 0.12136039775587193 1107 6 0.19319207384106107 1107 7 0.09033856883831959 1107 8 0.2576945368371142 1107 9 0.2821717571596736 1107 10-11 0.40054101966791933 1107 12-13 0.4908951822109202 1107 14-15 0.49719313999280956 1107 16-17 0.118592431983231 1107 18-19 0.3264850559504566 1107 20-21 0.4040401058528431 1107 22-23 0.47288198232230627 1107 24-25 0.4527572054307285 1107 26-27 0.4046609682496509 1107 28-29 0.3812275586899432 1107 30-31 0.42015901669824984 1107 32-33 0.45317056632125485 1107 34-35 0.20515382066176358 1107 36-37 0.24379338164723663 1107 38-39 0.3051342086983979 1107 40-41 0.5702838579527665 1107 42-43 0.11501014446712787 1107 44-45 0.3589866251310312 1107 46-47 0.5691682959204201 1107 48-49 0.5702070644619468 1107 50-51 0.6883986870563561 1107 52-53 0.450745119136144 1107 54-55 0.5518003765770132 1107 56-57 0.5991028923680162 1107 58-59 0.7014933631125828 1107 60-61 0.6267070416145515 1107 62-63 0.780234704029084 1107 64-65 0.457579687273892 1107 66-67 0.49147057154471696 1107 68-69 0.5553830836391782 1107 70-71 0.6443650628223025 1107 72-73 0.59512503568385 1107 74-75 0.5708667081939005 1107 76-77 0.5739848177841012 1107 78-79 0.4030197034408687 1107 80-81 0.5598820627820054 1107 82-83 0.6710723637112608 1107 84-85 0.533007014549419 1107 86-87 0.24696726785948542 1107 88-89 0.5022176761198729 1107 90-91 0.90772055383162 1107 92-93 0.6863330855354128 1107 94-95 0.6281494709099285 1107 96-97 0.9915054545018904 1107 98-99 1.0040798399642377 1107 100-101 0.9875590437133717 1108 1 0.11782333246958032 1108 2 0.20606192604456908 1108 3 0.11101494011771962 1108 4 0.10391328848577785 1108 5 0.170608192336843 1108 6 0.1719754176763928 1108 7 0.21468613237539103 1108 8 0.320720949355497 1108 9 0.23464366432203576 1108 10-11 0.18285369032534504 1108 12-13 0.18258022736949897 1108 14-15 0.2352937228535481 1108 16-17 0.3222970108532124 1108 18-19 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2316 94-95 -2.430275068260581 2316 96-97 -2.8374291973158243 2316 98-99 -2.5375452240398566 2316 100-101 -2.5201566500910353 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13515.0 3 2955.0 4 2429.0 5 2091.0 6 2026.0 7 2250.0 8 2639.0 9 3231.0 10 3823.0 11 4552.0 12 6198.0 13 9231.0 14 9042.0 15 8956.0 16 10839.0 17 14379.0 18 12443.0 19 8202.0 20 7875.0 21 7903.0 22 8435.0 23 9281.0 24 10675.0 25 12157.0 26 14184.0 27 17459.0 28 20865.0 29 25232.0 30 30724.0 31 38203.0 32 48296.0 33 63274.0 34 88112.0 35 132153.0 36 214322.0 37 339714.0 38 330925.0 39 61204.0 40 35.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.521956371790935 18.26624051108653 12.514828625798339 22.696974491324198 2 15.578899726063023 20.12742883077296 39.90821673403608 24.38545470912794 3 20.287598255131396 25.717557834092453 28.57403626516102 25.420807645615135 4 12.305007254308359 14.983631704921866 37.19806793483187 35.513293105937905 5 13.211181110434595 39.3336742310111 33.33310412340329 14.12204053515101 6 32.5817889041137 35.050646109838674 17.55823489993299 14.809330086114638 7 29.708153669395017 33.04074521830341 20.036930862774465 17.214170249527115 8 27.866062961460347 33.77719377099252 19.532193061474285 18.82455020607285 9 28.316014268692026 15.7236935056946 19.732668327929854 36.22762389768353 10-11 25.748235557386344 26.44691795271525 26.636629522370654 21.168216967527755 12-13 26.883209960895684 23.524633281771436 27.385743099233572 22.206413658099308 14-15 23.83768995459437 24.597555216115108 26.054836198104447 25.509918631186075 16-17 22.4197878807108 28.25152099889686 26.79712001910568 22.531571101286662 18-19 22.280106774024155 27.21206021354805 28.94798204596034 21.559850966467454 20-21 23.338638255951473 26.102500856372036 28.597950207904628 21.960910679771867 22-23 22.773913086965543 26.044029136045733 28.303716771230032 22.878341005758696 24-25 22.547187830086614 26.24728389222659 28.975598715962104 22.229929561724692 26-27 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26.872129759153434 27.474489627630085 22.54481416059307 52-53 22.896974185829485 27.04442934641773 27.651501944787082 22.4070945229657 54-55 22.471708613797453 27.023989889472638 27.708787163851344 22.79551433287856 56-57 22.704088442486288 27.534482287540463 27.041575342530283 22.71985392744297 58-59 22.630643418837774 26.778697585609123 27.544060749189757 23.046598246363338 60-61 22.665289522219016 26.742677587099728 27.782758708402643 22.809274182278614 62-63 23.132202542473745 26.52372723221702 27.7992663561718 22.544803869137436 64-65 22.95708320037388 26.76599470842305 27.856974152681847 22.419947938521222 66-67 22.577164821024464 27.238218625312392 27.567448077673657 22.617168475989484 68-69 22.67437331889409 27.17936025950433 27.592059589511784 22.55420683208979 70-71 22.622854449402233 27.123301780941635 27.351976282164614 22.90186748749152 72-73 22.631272497957085 27.07436581748885 27.00395518089755 23.290406503656516 74-75 22.56857589048462 27.474015099071163 27.013837674305822 22.94357133613839 76-77 22.78590771949979 27.037359347751057 27.461235739277544 22.715497193471606 78-79 22.716763477762758 26.959231306497568 27.45502178249662 22.868983433243052 80-81 22.418665885288835 27.577832361164894 27.227814035844766 22.775687717701505 82-83 22.53899150495385 27.41944247196453 27.12438180943949 22.917184213642138 84-85 22.595154742167576 27.08225833146729 26.95409036705157 23.368496559313563 86-87 22.708985542931515 26.84425611674196 27.393902353230853 23.052855987095672 88-89 23.10565655118864 26.597827138529656 27.333275537781333 22.963240772500374 90-91 22.409367447810958 26.96726080885948 27.565340769850437 23.05803097347913 92-93 22.375475653364653 27.274238020037995 27.398136434647196 22.952149891950153 94-95 22.30382481122793 27.133588410778327 27.291339458081342 23.2712473199124 96-97 22.46755966481643 26.82517211140593 27.004355728616723 23.702912495160913 98-99 22.448110790745336 26.796532012577984 27.009168516862736 23.746188679813944 100-101 22.720432379616945 26.92441614214725 27.01320793637886 23.341943541856946 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 312.0 1 234.5 2 302.0 3 466.0 4 399.5 5 379.0 6 776.0 7 1435.5 8 2073.0 9 2214.5 10 1842.5 11 1637.0 12 1663.5 13 1826.5 14 2014.5 15 2128.0 16 2328.5 17 2458.5 18 2530.0 19 2615.0 20 2701.0 21 3015.5 22 3431.5 23 3938.5 24 4477.0 25 5275.5 26 7019.5 27 9195.0 28 10896.5 29 12612.5 30 14766.5 31 17617.0 32 21687.5 33 25451.0 34 28787.0 35 32178.0 36 37322.5 37 41909.5 38 43451.0 39 45270.0 40 46616.0 41 47339.5 42 48234.5 43 50008.0 44 53424.0 45 57722.5 46 59463.0 47 60883.5 48 75573.0 49 75929.0 50 61756.5 51 56936.0 52 53415.5 53 52543.0 54 51888.5 55 49062.5 56 44211.5 57 39694.5 58 37146.0 59 35089.5 60 31258.5 61 26521.5 62 21553.5 63 16137.0 64 11596.0 65 8803.5 66 6415.0 67 5046.5 68 4012.5 69 2908.0 70 2196.5 71 1490.5 72 1149.0 73 999.0 74 727.5 75 506.5 76 338.5 77 196.0 78 118.5 79 73.5 80 43.0 81 34.0 82 33.5 83 25.0 84 19.0 85 19.5 86 20.0 87 15.0 88 12.0 89 15.0 90 15.5 91 16.5 92 13.0 93 9.0 94 13.5 95 14.5 96 13.0 97 12.5 98 8.0 99 4.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0453798499714657 2 0.01193877595668037 3 0.01043861562704514 4 0.005625601236132112 5 0.008188375132592296 6 0.006313174720548259 7 0.005188054473321837 8 0.00950101542102312 9 0.015189123337556702 10-11 0.0020002137728469733 12-13 0.008344641833595965 14-15 0.008594668555201837 16-17 0.01903328418224698 18-19 0.011813762595877434 20-21 0.0035628807828836707 22-23 0.010063575544636333 24-25 0.021471044717904227 26-27 0.007250774926570278 28-29 0.017283097131005877 30-31 0.014282776471735415 32-33 0.012470082740092847 34-35 0.2629968577891762 36-37 0.4666123691969579 38-39 0.4641746086613007 40-41 0.4830203728023432 42-43 0.4752382910923605 44-45 0.4872395737294423 46-47 0.4947716287178192 48-49 0.5020536569845903 50-51 0.5108358455809965 52-53 0.5006472566755572 54-55 0.48911477414148635 56-57 0.48402047968876677 58-59 0.48186399921491607 60-61 0.47789482500942293 62-63 0.477519784927014 64-65 0.4916150413575451 66-67 0.4672061826607719 68-69 0.4919900814399539 70-71 0.47564458451497 72-73 0.4838017063073616 74-75 0.4964905624288596 76-77 0.4836454396063579 78-79 0.48286410610133956 80-81 0.4856456533792049 82-83 0.4865207469048255 84-85 0.48614570682241665 86-87 0.487864640533457 88-89 0.5007410166961594 90-91 0.5252436354135348 92-93 0.5127110459930405 94-95 0.4852706132967961 96-97 0.45808020732215754 98-99 0.4503606322925763 100-101 0.45876778080657366 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1599829.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.33642772044849 #Duplication Level Percentage of deduplicated Percentage of total 1 66.3399772435992 25.43237742575438 2 14.261111546330191 10.934401440182818 3 6.294870537672642 7.23968548131204 4 3.6581195685280834 5.609569457265405 5 2.2329540774884826 4.280174129735897 6 1.4507282639610626 3.3369443540012997 7 1.0536023659254923 2.827394566317732 8 0.7917062517522176 2.4280951596900873 9 0.6028035585777582 2.079840154774083 >10 3.0271198038031493 20.49398185571168 >50 0.18084369678806844 4.753576100599616 >100 0.09979337222334214 6.851077320270154 >500 0.0034298456501754646 0.8898608954161055 >1k 0.002449889750125332 1.7479142102755414 >5k 4.899779500250664E-4 1.0951074486931949 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6038 0.3774153362640632 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5992 0.3745400289655957 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5460 0.34128647499201475 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4313 0.26959131257153107 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3058 0.19114542866768885 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2981 0.18633241427677583 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2464 0.1540164605092169 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2206 0.13788973696563822 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2000 0.1250133608029358 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1903 0.11895021280399341 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.25066804014679E-5 7 0.0 0.0 0.0 0.0 6.25066804014679E-5 8 0.0 0.0 0.0 0.0 1.250133608029358E-4 9 0.0 0.0 0.0 0.0 2.500267216058716E-4 10-11 0.0 0.0 0.0 3.125334020073395E-5 2.500267216058716E-4 12-13 0.0 0.0 0.0 1.250133608029358E-4 2.500267216058716E-4 14-15 0.0 0.0 0.0 2.1877338140513768E-4 2.500267216058716E-4 16-17 0.0 0.0 0.0 5.93813463813945E-4 3.7504008240880747E-4 18-19 0.0 0.0 0.0 7.500801648176149E-4 5.000534432117432E-4 20-21 0.0 0.0 0.0 0.001312640288430826 5.000534432117432E-4 22-23 0.0 0.0 0.0 0.0029690673190697256 5.313067834124772E-4 24-25 0.0 0.0 0.0 0.004781761050712294 5.625601236132112E-4 26-27 0.0 0.0 0.0 0.005594347895931377 5.625601236132112E-4 28-29 0.0 0.0 0.0 0.007375788287373213 5.625601236132112E-4 30-31 0.0 0.0 0.0 0.012063789317483305 5.93813463813945E-4 32-33 0.0 0.0 0.0 0.019970884388268995 7.813335050183489E-4 34-35 0.0 0.0 0.0 0.032284700427358176 8.125868452190828E-4 36-37 0.0 0.0 0.0 0.05406827854726974 8.125868452190828E-4 38-39 0.0 0.0 0.0 0.08660300569623378 8.750935256205507E-4 40-41 0.0 0.0 0.0 0.12588845432855636 8.750935256205507E-4 42-43 0.0 0.0 0.0 0.1630174224870283 9.063468658212846E-4 44-45 0.0 0.0 0.0 0.2023966311399531 9.688535462227526E-4 46-47 0.0 0.0 0.0 0.2514956285953061 0.0010626135668249544 48-49 0.0 0.0 0.0 0.31253340200733953 0.0010626135668249544 50-51 0.0 0.0 0.0 0.38110323040774985 0.0010626135668249544 52-53 0.0 0.0 0.0 0.4398282566449289 0.00118762692762789 54-55 0.0 0.0 0.0 0.5012098167991704 0.001312640288430826 56-57 0.0 0.0 0.0 0.5775929802497641 0.001312640288430826 58-59 0.0 0.0 0.0 0.6634459057811803 0.001312640288430826 60-61 0.0 0.0 0.0 0.7685821422164494 0.001312640288430826 62-63 0.0 0.0 0.0 0.8694366710442178 0.001375146968832294 64-65 0.0 0.0 0.0 0.9678534393363292 0.001375146968832294 66-67 0.0 0.0 0.0 1.083209518017238 0.001375146968832294 68-69 0.0 0.0 0.0 1.2156299204477479 0.001375146968832294 70-71 0.0 0.0 0.0 1.3705839811629867 0.0014064003090330278 72-73 0.0 0.0 0.0 1.5301635362279344 0.0016876803708396335 74-75 0.0 0.0 0.0 1.6843987701185563 0.0016876803708396335 76-77 0.0 0.0 0.0 1.8510415800688698 0.0017189337110403674 78-79 0.0 0.0 0.0 2.05203181089979 0.0017501870512411015 80-81 0.0 0.0 0.0 2.2787747940561145 0.0018126937316425692 82-83 0.0 0.0 0.0 2.514956285953061 0.0021252271336499088 84-85 0.0 0.0 0.0 2.754544391931888 0.0021252271336499088 86-87 0.0 0.0 0.0 3.0169786895974506 0.002156480473850643 88-89 0.0 0.0 0.0 3.310510060762744 0.002187733814051377 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 11960 0.0 28.418434 2 GTACATG 12250 0.0 28.333288 1 TCATACG 70 0.008533846 27.050095 2 ACATGGG 12255 0.0 26.767834 3 TACCTGG 1210 0.0 26.602987 2 GTGTACG 280 0.0 25.3666 1 TAAGGTG 495 0.0 24.861898 5 GTATAGG 445 0.0 24.473547 1 CATGGGG 4380 0.0 24.423082 4 ATGGGAT 2865 0.0 24.28616 5 CATGGGA 7915 0.0 23.86097 4 TATTCCG 200 2.1334927E-8 23.666613 5 GTACGAT 105 0.0022411367 22.541042 3 GTACCTG 1590 0.0 22.037569 1 GAGTACT 5685 0.0 21.653316 12-13 GTCTACG 110 0.0029307974 21.523178 1 TGGGATA 800 0.0 21.297956 6 AGTACAT 8560 0.0 21.069685 2 AGTACTT 5875 0.0 21.033627 12-13 GTACTTT 5895 0.0 20.680841 14-15 >>END_MODULE