FastQCFastQC Report
Thu 26 May 2016
SRR936321_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936321_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1599829
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA60690.3793530433565087No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60270.3767277627796471No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA55080.3442867956512852No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44010.27509190044686027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32350.20220911109874867No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT31490.19683353658422245No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG25380.15864195485892554No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA21310.1332017359355281No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT20220.1263885077717681No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC19790.12370072051450498No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGC300.003959518431.65436778-79
TACCTGG11950.029.3766062
TACATGG117450.028.1121482
GTACATG120250.027.5289821
CCGTACG350.00840968927.13231578-79
ACATGGG118950.026.8370113
CATGGGA76500.023.8096644
GTACGAT1000.001667506123.716663
TAGACTC8200.022.5604555
ATGGGAT26100.022.1725885
CATGGGT11750.021.7991434
CATGGGG40600.021.6137054
ATGGGAG28200.021.1942735
AGTACAT84950.020.3829632
ACACCGA1656.3918786E-520.1238566
GTACCTG17750.020.0968931
CTAGACT11050.019.7459984
AGTACTT59850.019.7347812-13
GAGTACT57350.019.68523812-13
GCGTGTA1708.040748E-519.5319779
Produced by FastQC (version 0.11.5)