##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936321_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1599829 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.4535259705881 34.0 31.0 34.0 31.0 34.0 2 32.575487130187035 34.0 31.0 34.0 31.0 34.0 3 32.663435904712316 34.0 31.0 34.0 31.0 34.0 4 36.15438087445596 37.0 35.0 37.0 35.0 37.0 5 36.12411701500598 37.0 35.0 37.0 35.0 37.0 6 36.173836078730915 37.0 36.0 37.0 35.0 37.0 7 36.118282641457306 37.0 36.0 37.0 35.0 37.0 8 36.103421053125054 37.0 36.0 37.0 35.0 37.0 9 37.85453757870373 39.0 38.0 39.0 35.0 39.0 10-11 37.79497683814958 39.0 38.0 39.0 35.0 39.0 12-13 37.743164113164596 39.0 38.0 39.0 35.0 39.0 14-15 39.04608179999238 40.0 38.0 41.0 36.0 41.0 16-17 38.97752416039464 40.0 38.0 41.0 35.5 41.0 18-19 39.052419977385085 40.0 38.5 41.0 36.0 41.0 20-21 39.014924407546054 40.0 38.5 41.0 35.0 41.0 22-23 38.908712743674485 40.0 38.0 41.0 35.0 41.0 24-25 39.04930058149965 40.0 39.0 41.0 35.0 41.0 26-27 38.94089305794557 40.0 38.0 41.0 35.0 41.0 28-29 38.848212215180496 40.0 38.0 41.0 35.0 41.0 30-31 38.727015199749474 40.0 38.0 41.0 35.0 41.0 32-33 38.5635405408953 40.0 38.0 41.0 34.0 41.0 34-35 38.42265392113782 40.0 38.0 41.0 34.0 41.0 36-37 38.26246086300473 40.0 38.0 41.0 34.0 41.0 38-39 38.111873831515744 40.0 38.0 41.0 33.5 41.0 40-41 37.95952379910603 40.0 37.5 41.0 33.0 41.0 42-43 37.969753017353725 40.0 38.0 41.0 33.0 41.0 44-45 37.80203259223329 40.0 37.5 41.0 33.0 41.0 46-47 37.54534609636405 40.0 37.0 41.0 33.0 41.0 48-49 37.276024812651855 40.0 37.0 41.0 32.0 41.0 50-51 37.076472548003565 40.0 37.0 41.0 31.5 41.0 52-53 36.847619964383696 40.0 36.0 41.0 31.0 41.0 54-55 36.65809095847119 39.5 36.0 41.0 31.0 41.0 56-57 36.43811494853513 39.0 35.0 41.0 31.0 41.0 58-59 36.19184019042035 39.0 35.0 41.0 30.0 41.0 60-61 35.92309615590166 39.0 35.0 41.0 30.0 41.0 62-63 35.63118777069299 38.0 35.0 40.0 29.0 41.0 64-65 35.2479911915586 37.5 35.0 40.0 29.0 41.0 66-67 34.90533582026579 37.0 34.0 40.0 28.0 41.0 68-69 34.558060892757915 36.5 34.0 39.0 28.0 41.0 70-71 34.18558483437917 36.0 34.0 39.0 28.0 40.5 72-73 33.78101878388253 35.0 34.0 38.5 27.5 40.0 74-75 33.359482169656886 35.0 33.5 37.0 26.5 39.5 76-77 32.60985236547156 35.0 32.5 36.5 26.0 39.0 78-79 32.63756845262837 35.0 33.0 36.0 26.0 39.0 80-81 32.48713768784039 35.0 33.0 36.0 26.0 37.5 82-83 32.22095267681733 35.0 33.0 35.5 26.0 37.0 84-85 31.949590549990035 35.0 33.0 35.0 25.5 37.0 86-87 31.563502724353665 35.0 32.5 35.0 24.5 36.0 88-89 31.06606612331693 35.0 32.0 35.0 21.5 36.0 90-91 30.852743324442798 34.5 32.0 35.0 20.0 36.0 92-93 30.605613787473537 34.0 31.5 35.0 19.5 35.0 94-95 30.48634072766527 34.0 31.5 35.0 19.0 35.0 96-97 30.262258966427037 34.0 31.0 35.0 15.5 35.0 98-99 29.856302142291455 34.0 31.0 35.0 2.0 35.0 100-101 26.626658849164507 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10170172285368295 1101 2 0.0412255911705941 1101 3 0.10199554673674527 1101 4 0.12121566875957512 1101 5 0.04202771154795215 1101 6 0.07403442163175811 1101 7 0.16604169981228267 1101 8 0.05021507334119235 1101 9 0.024103010042807682 1101 10-11 0.09795707243272034 1101 12-13 0.19491448642999387 1101 14-15 0.3053410704334709 1101 16-17 0.27977755513090585 1101 18-19 0.3000055938421866 1101 20-21 0.3778712936524755 1101 22-23 0.38585089598675637 1101 24-25 0.2550540525217144 1101 26-27 0.34670518441383535 1101 28-29 0.30347088543604883 1101 30-31 0.24922333441143252 1101 32-33 0.339434650866167 1101 34-35 0.2471235542526884 1101 36-37 0.2790056607972602 1101 38-39 0.24343220984795266 1101 40-41 0.3894614685627147 1101 42-43 0.3483000496700086 1101 44-45 0.2940171106780056 1101 46-47 0.4210103472774307 1101 48-49 0.27165182235580687 1101 50-51 0.20107772918656508 1101 52-53 0.36571192713259393 1101 54-55 0.37680391426723503 1101 56-57 0.3255467889139467 1101 58-59 0.1976621910359171 1101 60-61 0.30521014771814947 1101 62-63 0.3769564367976699 1101 64-65 0.37843698869492215 1101 66-67 0.30838454047659525 1101 68-69 0.35809086092142195 1101 70-71 0.3261731058788584 1101 72-73 0.26738878712331626 1101 74-75 0.4217391742478611 1101 76-77 0.21436418581351546 1101 78-79 0.11955237297397048 1101 80-81 0.12708062571957157 1101 82-83 0.14412044347738373 1101 84-85 0.2587142015847661 1101 86-87 0.29384078047675644 1101 88-89 0.3056790718317757 1101 90-91 0.3778813236674097 1101 92-93 0.43388414101558226 1101 94-95 0.19997815452829926 1101 96-97 0.10063178745514278 1101 98-99 0.24046171284307505 1101 100-101 0.5178011967657277 1102 1 -0.032611837205024585 1102 2 0.10942319097014774 1102 3 0.07561890855136255 1102 4 0.00862765544444244 1102 5 0.011996059991197683 1102 6 0.02703569485269952 1102 7 0.07098425146977405 1102 8 0.06079712450524255 1102 9 0.007830837334729779 1102 10-11 0.06055710209007259 1102 12-13 0.13644322082792826 1102 14-15 0.12303909719667416 1102 16-17 0.13990809862750808 1102 18-19 0.16409871793777597 1102 20-21 0.02638280121765746 1102 22-23 0.15785946593975808 1102 24-25 0.13374728808804548 1102 26-27 0.1727379506780835 1102 28-29 0.16440345585948535 1102 30-31 0.04705042180348329 1102 32-33 0.14100240002630215 1102 34-35 0.06861797815856363 1102 36-37 0.09153001306527386 1102 38-39 0.16382598793935443 1102 40-41 0.06996073479883336 1102 42-43 0.1980817101751029 1102 44-45 0.2647499030202738 1102 46-47 0.1705851732961463 1102 48-49 0.19158591144036308 1102 50-51 0.26782237424426114 1102 52-53 0.1612940038633326 1102 54-55 0.036820686012980275 1102 56-57 0.2055354884389189 1102 58-59 0.08301017919342257 1102 60-61 -0.027688296293369774 1102 62-63 0.04587782946617125 1102 64-65 -0.04182205296343966 1102 66-67 0.003582605589826926 1102 68-69 -0.051416622447447935 1102 70-71 -0.16658231809348223 1102 72-73 -0.2233136319964686 1102 74-75 -0.10817589322262222 1102 76-77 -0.4101105466311239 1102 78-79 -0.19842126265985627 1102 80-81 0.017416929901770573 1102 82-83 -0.08284473981018436 1102 84-85 0.11188421493058343 1102 86-87 -0.058952124256663296 1102 88-89 -0.1612611583706638 1102 90-91 0.11942923307952924 1102 92-93 0.027100787995898656 1102 94-95 -0.019470423196981557 1102 96-97 -0.2730636724128317 1102 98-99 0.046774894514832965 1102 100-101 0.4175115262180391 1103 1 0.09567015413441027 1103 2 0.08023174106434539 1103 3 0.19981425472759184 1103 4 0.05169870566194845 1103 5 0.061753899385941224 1103 6 0.07132297328314507 1103 7 0.2602068715390189 1103 8 0.14197764512588407 1103 9 0.20390793416937925 1103 10-11 0.1810001918818429 1103 12-13 0.24132046364941573 1103 14-15 0.3494592631087414 1103 16-17 0.3254021358541479 1103 18-19 0.20282764601952863 1103 20-21 0.25590124192036967 1103 22-23 0.35896077883493405 1103 24-25 0.2645579096171531 1103 26-27 0.29574419457474477 1103 28-29 0.3370822157604536 1103 30-31 0.3888268252006313 1103 32-33 0.3590342409401046 1103 34-35 0.31742542348747094 1103 36-37 0.14751645996256713 1103 38-39 0.28898208535597547 1103 40-41 0.40827389630171496 1103 42-43 0.2551694699675622 1103 44-45 0.37799229049696237 1103 46-47 0.3589654098342834 1103 48-49 0.49759849787345445 1103 50-51 0.48530725770139327 1103 52-53 0.47440174807071145 1103 54-55 0.5189792198316283 1103 56-57 0.5130350692199457 1103 58-59 0.5384698897843805 1103 60-61 0.4564247843249092 1103 62-63 0.39411908258565376 1103 64-65 0.5655680376864964 1103 66-67 0.5053275429042614 1103 68-69 0.4480956039505273 1103 70-71 0.2765558071592764 1103 72-73 0.3089800382902794 1103 74-75 0.4523918772229294 1103 76-77 0.4165478342958906 1103 78-79 0.42931278482532065 1103 80-81 0.4765721790164861 1103 82-83 0.4956483406108134 1103 84-85 0.47654257612964557 1103 86-87 0.4973741366060409 1103 88-89 0.5144330189244357 1103 90-91 0.5790426638689432 1103 92-93 0.7102555833677222 1103 94-95 0.5031604964214793 1103 96-97 0.5078870175722727 1103 98-99 0.5075138049400714 1103 100-101 1.0731670367281048 1104 1 -0.01954012287625062 1104 2 0.04228111853152683 1104 3 0.01704288980734958 1104 4 0.030655275644818403 1104 5 -0.02281161455515246 1104 6 0.12501303287204024 1104 7 0.07076404818276671 1104 8 0.09286757011308566 1104 9 0.049076906001211285 1104 10-11 0.044858017416324 1104 12-13 0.09261085726403451 1104 14-15 0.10841356430958626 1104 16-17 0.1529620703073249 1104 18-19 0.20673843522202873 1104 20-21 0.11322869294375693 1104 22-23 0.028110633794945272 1104 24-25 0.04725649239099994 1104 26-27 0.15177853516468787 1104 28-29 0.13496281109648578 1104 30-31 0.032112326634823773 1104 32-33 0.11722044502943874 1104 34-35 0.11025535730195912 1104 36-37 -0.1553754745172924 1104 38-39 -0.06050210037529524 1104 40-41 -0.02017629678307742 1104 42-43 0.11011827461494761 1104 44-45 0.1968777084247364 1104 46-47 0.12504635578292778 1104 48-49 0.041795420528004 1104 50-51 0.25003670544361967 1104 52-53 0.09639382449244494 1104 54-55 0.11408384595446108 1104 56-57 -0.0576086547855752 1104 58-59 0.030261283855608667 1104 60-61 -0.07068310960666935 1104 62-63 0.09041114918870363 1104 64-65 0.0938853926298222 1104 66-67 0.02012896251359564 1104 68-69 0.18257756379689738 1104 70-71 0.21066723104879514 1104 72-73 0.19821962453306696 1104 74-75 0.15856077183991601 1104 76-77 0.24275841539960652 1104 78-79 0.08880816194361074 1104 80-81 0.11557606931038578 1104 82-83 0.05930936360272199 1104 84-85 0.201269249856864 1104 86-87 0.2307977286981 1104 88-89 0.21186042981516096 1104 90-91 0.4415623369736785 1104 92-93 0.35200417562660036 1104 94-95 0.3843683082765317 1104 96-97 0.32924092755119716 1104 98-99 0.1563187383017457 1104 100-101 0.29273914252960864 1105 1 -0.01673131383981996 1105 2 0.04336238572697226 1105 3 0.05587506599912473 1105 4 0.008434751453478384 1105 5 0.0431912550147473 1105 6 0.09095979070455229 1105 7 0.0806280220997806 1105 8 -0.012303961949086784 1105 9 0.0530613456962854 1105 10-11 -0.019684289867022642 1105 12-13 0.09381548742600643 1105 14-15 0.005397431839853084 1105 16-17 0.006249210040280673 1105 18-19 -0.030070818473454608 1105 20-21 -0.07329450136534632 1105 22-23 -0.14090261437498697 1105 24-25 0.022907036407872283 1105 26-27 0.0588853007488126 1105 28-29 -0.00199171630136874 1105 30-31 0.00946486531118751 1105 32-33 -0.003837060985659946 1105 34-35 0.0447650424455972 1105 36-37 0.08413464561889583 1105 38-39 0.21321155300515215 1105 40-41 0.24077412281211963 1105 42-43 0.23221976274977152 1105 44-45 0.14426064134243433 1105 46-47 0.34180199261892596 1105 48-49 0.21075366170352083 1105 50-51 0.16007311132803181 1105 52-53 0.24287238718499538 1105 54-55 0.20245614986372829 1105 56-57 0.30420111675817196 1105 58-59 0.26325180930359693 1105 60-61 0.2232043014327516 1105 62-63 0.17471316566686568 1105 64-65 0.15928916928577763 1105 66-67 0.17501185745575754 1105 68-69 0.1581940449051018 1105 70-71 0.0224797874654854 1105 72-73 -0.04683791646555591 1105 74-75 0.11190517408419964 1105 76-77 0.013880756018565421 1105 78-79 0.040553678873564536 1105 80-81 0.14329130891762532 1105 82-83 0.018193131291845077 1105 84-85 -0.024378877534736887 1105 86-87 0.0942853286485672 1105 88-89 0.22466923424849483 1105 90-91 0.2875791170989821 1105 92-93 0.3441220645916445 1105 94-95 0.12271315471955901 1105 96-97 0.11659286322201368 1105 98-99 0.02423727823659405 1105 100-101 0.23723179161582308 1106 1 0.023208216010139893 1106 2 -0.01059404240958628 1106 3 -0.02269846970238376 1106 4 -0.02803115204355322 1106 5 -0.020033407194368635 1106 6 -0.024931467036907407 1106 7 0.06194808977900834 1106 8 0.0717857713061818 1106 9 -0.12948605800562518 1106 10-11 -0.01718106416469567 1106 12-13 0.011457187440626626 1106 14-15 0.10897542838057461 1106 16-17 -0.053941857342152844 1106 18-19 0.04529539625117707 1106 20-21 0.1006766587487391 1106 22-23 -0.04969907547243224 1106 24-25 8.391079140039892E-4 1106 26-27 -0.04589398410020351 1106 28-29 -0.11717529216388556 1106 30-31 -0.02796023069772957 1106 32-33 0.06997010164263884 1106 34-35 0.15590315271754918 1106 36-37 0.12354290969340553 1106 38-39 0.20196620326191095 1106 40-41 -0.037718534960369254 1106 42-43 -5.613978933425301E-4 1106 44-45 0.08956739016625193 1106 46-47 0.11695148677321043 1106 48-49 0.1009425950563454 1106 50-51 0.28635677945594296 1106 52-53 0.10259446121071392 1106 54-55 0.1600866955386877 1106 56-57 0.335952877108447 1106 58-59 0.297717732526948 1106 60-61 0.19312053628718928 1106 62-63 0.24947884831872358 1106 64-65 0.27582937825928155 1106 66-67 0.22570761387510885 1106 68-69 0.2257690340780414 1106 70-71 0.18551740825559904 1106 72-73 0.14681249361782278 1106 74-75 0.24106328146892508 1106 76-77 0.29570335180556384 1106 78-79 0.16520589032899835 1106 80-81 0.17942679648511017 1106 82-83 0.27627285433425186 1106 84-85 0.38342073170471735 1106 86-87 0.4469545693016528 1106 88-89 0.5454679082808553 1106 90-91 0.6582744072597606 1106 92-93 0.573761583950791 1106 94-95 0.5399852103190987 1106 96-97 0.6704472972961888 1106 98-99 0.47019051250193655 1106 100-101 0.6518542432213259 1107 1 -0.0252684565903607 1107 2 0.08414245810962484 1107 3 0.05344101257027489 1107 4 0.09999881383688347 1107 5 0.11563081176424816 1107 6 0.008448224403529991 1107 7 0.10409595359742951 1107 8 0.08327052876056484 1107 9 0.09822681842220504 1107 10-11 0.008074832586522973 1107 12-13 0.1044840009697765 1107 14-15 0.18461563383969803 1107 16-17 0.17146099460859432 1107 18-19 0.09650858791412986 1107 20-21 0.047821926513165636 1107 22-23 0.08351697184992446 1107 24-25 0.07621182709513619 1107 26-27 0.23631655587902856 1107 28-29 0.15655032347178377 1107 30-31 -0.05133167039510056 1107 32-33 0.10213567425797976 1107 34-35 0.048641618820497 1107 36-37 0.09515737713147843 1107 38-39 0.051902229074102024 1107 40-41 -0.03917047087491454 1107 42-43 -0.08825841984853611 1107 44-45 -0.07475216553764596 1107 46-47 0.027448352965166123 1107 48-49 -0.11425776468256288 1107 50-51 0.0035936508400098433 1107 52-53 0.08327981946617058 1107 54-55 0.011754196651281745 1107 56-57 -0.08693643827502484 1107 58-59 -0.11397563640940689 1107 60-61 0.015928724983233167 1107 62-63 -0.0326904329451807 1107 64-65 0.014051173986914023 1107 66-67 -0.14182224074586713 1107 68-69 -0.08217164608811345 1107 70-71 0.0421721145306293 1107 72-73 -0.026608549254156344 1107 74-75 -0.017528730102057466 1107 76-77 -0.14532774521268976 1107 78-79 -0.26316712554401533 1107 80-81 -0.04215753818333212 1107 82-83 0.05676814435057054 1107 84-85 -0.025830028568236685 1107 86-87 0.014954495128684187 1107 88-89 0.14135408985628573 1107 90-91 0.06344519052633757 1107 92-93 0.36391373125831095 1107 94-95 0.21387029784320433 1107 96-97 -0.06546910976507547 1107 98-99 -0.002102174043084659 1107 100-101 0.25543262550881707 1108 1 -0.039045761778346844 1108 2 0.04875204643141018 1108 3 -0.008390797973724773 1108 4 0.05083897346720789 1108 5 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-0.9205988775486524 2316 94-95 -0.9546252858741511 2316 96-97 -0.8807212859043503 2316 98-99 -1.0614083696396044 2316 100-101 -1.0317585547267178 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 15.0 5 25.0 6 68.0 7 115.0 8 298.0 9 485.0 10 948.0 11 1483.0 12 2415.0 13 3805.0 14 4693.0 15 6421.0 16 8369.0 17 12167.0 18 10744.0 19 6084.0 20 5076.0 21 5427.0 22 5647.0 23 6074.0 24 7212.0 25 8872.0 26 10762.0 27 13685.0 28 17345.0 29 21706.0 30 27253.0 31 34508.0 32 43142.0 33 56132.0 34 78150.0 35 117953.0 36 201859.0 37 370448.0 38 428267.0 39 82133.0 40 39.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.80848756823837 18.60633903892799 12.96532143327212 22.61985195956152 2 15.83406637170078 20.39653468224897 39.58500963109258 24.18438931495767 3 20.126776049190255 25.75581515274445 28.919653287945152 25.197755510120146 4 12.516212670229132 15.279132957334815 37.09002649658182 35.11462787585423 5 13.072959672565004 39.28326089850852 33.5632745749702 14.080504853956267 6 32.57891937200788 35.340714538866344 17.415923826858997 14.66444226226678 7 29.54922057294873 33.10785090156511 20.028328027557947 17.314600497928215 8 27.777093676886714 34.035887585485696 19.42045056065367 18.766568176973912 9 28.0487477099115 15.78137413436061 19.68441627198907 36.48546188373882 10-11 25.659652748217145 26.565786504598332 26.628574857530758 21.14598588965377 12-13 26.75018814522561 23.605850128888857 27.46217093337534 22.181790792510196 14-15 23.667935760634418 24.67819998262314 26.140387503914482 25.513476752827955 16-17 22.306658778281687 28.288861156611006 26.92027011780384 22.484209947303462 18-19 22.1724634320293 27.224128328715132 29.059105691920823 21.544302547334745 20-21 23.232427725846865 26.06360619278879 28.698138581729225 22.005827499635117 22-23 22.686830905052975 26.03121958659332 28.447477824192465 22.834471684161244 24-25 22.477931588863093 26.255439749268344 29.06395057375994 22.202678088108623 26-27 22.652326881346728 26.08774330904996 29.136109299243056 22.123820510360257 28-29 22.171670211419364 26.168025966029756 29.54754786978855 22.112755952762328 30-31 22.17670291369066 26.50290550218635 29.186787551892895 22.133604032230085 32-33 21.906458833523253 26.416340950700988 28.947955662207175 22.72924455356858 34-35 22.641391495023296 26.111909298917464 28.862120340329085 22.384578865730155 36-37 23.04475856154537 25.81618148951012 28.98008410904172 22.15897583990279 38-39 22.885245922133382 25.92045194929072 29.039417111428715 22.154885017147183 40-41 22.49953900919794 26.088297709422655 29.30088791516519 22.11127536621422 42-43 22.445982995098056 26.357441738645583 28.82416516332932 22.372410102927038 44-45 22.282172149464827 26.744870014958483 28.31701110353359 22.655946732043102 46-47 22.589946812101935 26.636740659609952 27.889606979692577 22.883705548595536 48-49 22.6942970198366 26.67871556117226 27.798593830244993 22.82839358874614 50-51 23.05340700851928 26.822368476572063 27.568809504386536 22.555415010522122 52-53 22.873468948902246 26.954219431174742 27.775051602845004 22.39726001707801 54-55 22.4344715957374 26.914221367821344 27.8090711995057 22.842235836935558 56-57 22.70494590533246 27.451106021547854 27.10549772844902 22.738450344670667 58-59 22.579637980200047 26.70405462676006 27.688613965978288 23.0276934270616 60-61 22.631261145875722 26.701658387543 27.849296821429085 22.817783645152197 62-63 23.10850097104128 26.505551530819854 27.86235278895432 22.523594709184543 64-65 22.963392874723056 26.711115653312905 27.874096416984123 22.451395054979915 66-67 22.519750548339854 27.191531094885768 27.620389429120234 22.668328927654144 68-69 22.578045084207464 27.213323178078152 27.67159104866553 22.537040689048858 70-71 22.586494589566176 27.146460660865824 27.352295609739937 22.91474913982806 72-73 22.6177565390782 27.03073187044671 26.9858201468304 23.365691443644696 74-75 22.502842491291254 27.438870029234373 27.072674642102374 22.985612837371995 76-77 22.75947805337834 27.050569450402858 27.44747810348121 22.742474392737588 78-79 22.66889595065206 26.947732071685415 27.501006650620745 22.88236532704178 80-81 22.407688922670722 27.466014671760615 27.26742834140097 22.8588680641677 82-83 22.479277472779906 27.47156102308434 27.10395923564331 22.945202268492444 84-85 22.50391763557957 27.023715837498912 26.948269708347894 23.524096818573625 86-87 22.657816270097932 26.824486848161754 27.3541526051939 23.163544276546418 88-89 22.9741136880017 26.60220265826792 27.276248407236814 23.147435246493565 90-91 22.368395889692472 26.979653808448784 27.570517569316845 23.0814327325419 92-93 22.34729737057982 27.28367852381581 27.302768409593092 23.06625569601128 94-95 22.101431383420607 27.1293695824616 27.294704712707553 23.474494321410244 96-97 22.32523571055085 26.8466714860477 26.98315810726467 23.844934696136775 98-99 22.277844297153774 26.9169691292796 26.86277432991756 23.942412243649066 100-101 22.460832995794515 26.99072893382739 27.174312545942787 23.37412552443531 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 268.0 1 201.0 2 287.0 3 451.5 4 352.0 5 326.0 6 682.0 7 1339.0 8 1959.5 9 2043.5 10 1645.0 11 1320.0 12 1279.5 13 1379.5 14 1495.0 15 1518.5 16 1605.5 17 1766.5 18 1857.5 19 2016.5 20 2267.5 21 2591.5 22 3054.0 23 3710.0 24 4220.0 25 5052.0 26 6826.5 27 9123.5 28 10780.0 29 12494.5 30 15016.0 31 17865.0 32 21987.5 33 25758.0 34 29197.5 35 32676.5 36 37897.5 37 42684.0 38 44145.5 39 45840.5 40 47135.0 41 47941.5 42 48918.5 43 50647.5 44 54098.0 45 57842.0 46 59527.0 47 61511.0 48 76689.5 49 76523.5 50 62009.0 51 57584.5 52 53515.5 53 52627.5 54 52166.0 55 49198.0 56 44140.0 57 39672.5 58 37170.5 59 35203.0 60 31056.5 61 26159.0 62 21523.5 63 15958.5 64 11089.0 65 8244.5 66 6085.5 67 4905.5 68 3909.0 69 2781.5 70 2053.5 71 1331.5 72 1038.5 73 971.0 74 683.5 75 445.0 76 293.5 77 134.0 78 77.5 79 46.5 80 26.5 81 15.5 82 10.0 83 9.5 84 7.0 85 2.0 86 2.0 87 1.0 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2699663526539399 2 0.020127151089272665 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.250668040146791E-4 12-13 6.250668040146792E-5 14-15 0.0 16-17 5.313067834124772E-4 18-19 0.0 20-21 3.125334020073396E-5 22-23 0.0 24-25 0.0019377070924455051 26-27 0.0023440005150550465 28-29 0.0029065606386682575 30-31 0.0012188802678286242 32-33 0.0012501336080293583 34-35 0.001531413669835964 36-37 6.250668040146792E-5 38-39 2.1877338140513766E-4 40-41 8.750935256205506E-4 42-43 0.0034378674220807348 44-45 0.0042504542672998176 46-47 0.013563949647118535 48-49 0.01472032323454569 50-51 0.006531948101953396 52-53 0.006938241524562938 54-55 4.6880010301100924E-4 56-57 0.0025627738964601843 58-59 0.0016876803708396337 60-61 0.001375146968832294 62-63 0.0 64-65 0.0010938669070256883 66-67 0.0 68-69 3.125334020073396E-5 70-71 2.1877338140513766E-4 72-73 0.0015001603296352297 74-75 0.0 76-77 0.19464580277017107 78-79 0.10673015678550644 80-81 0.001312640288430826 82-83 0.0 84-85 7.500801648176148E-4 86-87 0.12091917323663966 88-89 0.589594262886846 90-91 0.31006438813148157 92-93 0.296500438484363 94-95 0.0030003206592704593 96-97 0.0024065071954565143 98-99 0.002781547277865322 100-101 8.125868452190828E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1599829.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.027323312183526 #Duplication Level Percentage of deduplicated Percentage of total 1 63.58412174210443 22.90765711524034 2 15.198939775585007 10.951542345948143 3 6.8777959885078825 7.433657392596372 4 3.86741186593201 5.5732999070122995 5 2.4705355455181897 4.450339142631276 6 1.625217965204209 3.513135185110864 7 1.1721192284662731 2.955982288306707 8 0.8614175996787018 2.4827656296343736 9 0.6444495934689791 2.0896014476080937 >10 3.3685619903982076 21.370214681948553 >50 0.21327577621295146 5.258716316618944 >100 0.1092184798459754 7.165149991608157 >500 0.0038139469925071094 0.9485406814907007 >1k 0.0026004184039821196 1.799897633449596 >5k 5.20083680796424E-4 1.099500240795568 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6069 0.3793530433565087 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6027 0.3767277627796471 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5508 0.3442867956512852 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4401 0.27509190044686027 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3235 0.20220911109874867 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3149 0.19683353658422245 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2538 0.15864195485892554 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2131 0.1332017359355281 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2022 0.1263885077717681 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1979 0.12370072051450498 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.25066804014679E-5 7 0.0 0.0 0.0 0.0 6.25066804014679E-5 8 0.0 0.0 0.0 0.0 6.25066804014679E-5 9 0.0 0.0 0.0 0.0 1.250133608029358E-4 10-11 0.0 0.0 0.0 3.125334020073395E-5 1.250133608029358E-4 12-13 0.0 0.0 0.0 1.250133608029358E-4 1.250133608029358E-4 14-15 0.0 0.0 0.0 2.1877338140513768E-4 1.875200412044037E-4 16-17 0.0 0.0 0.0 5.93813463813945E-4 2.500267216058716E-4 18-19 0.0 0.0 0.0 7.500801648176149E-4 2.500267216058716E-4 20-21 0.0 0.0 0.0 0.001312640288430826 2.500267216058716E-4 22-23 0.0 0.0 0.0 0.0029690673190697256 3.750400824088074E-4 24-25 0.0 0.0 0.0 0.004844267731113763 5.625601236132112E-4 26-27 0.0 0.0 0.0 0.0057193612567343136 6.25066804014679E-4 28-29 0.0 0.0 0.0 0.007500801648176149 6.25066804014679E-4 30-31 0.0 0.0 0.0 0.012157549338085508 6.25066804014679E-4 32-33 0.0 0.0 0.0 0.019970884388268995 6.25066804014679E-4 34-35 0.0 0.0 0.0 0.03215968706655524 7.500801648176149E-4 36-37 0.0 0.0 0.0 0.05419329190807268 7.500801648176149E-4 38-39 0.0 0.0 0.0 0.0874468458816536 8.125868452190828E-4 40-41 0.0 0.0 0.0 0.1273886146581916 8.125868452190828E-4 42-43 0.0 0.0 0.0 0.1645175828166635 8.125868452190828E-4 44-45 0.0 0.0 0.0 0.2043343382323986 8.125868452190828E-4 46-47 0.0 0.0 0.0 0.254089655831967 8.125868452190828E-4 48-49 0.0 0.0 0.0 0.31575249604801514 8.125868452190828E-4 50-51 0.0 0.0 0.0 0.3845410978298306 8.125868452190828E-4 52-53 0.0 0.0 0.0 0.4437661775102214 8.438401854198168E-4 54-55 0.0 0.0 0.0 0.5049289642830578 8.750935256205507E-4 56-57 0.0 0.0 0.0 0.5821247145788706 8.750935256205507E-4 58-59 0.0 0.0 0.0 0.6686027069143015 9.063468658212846E-4 60-61 0.0 0.0 0.0 0.7748015569163954 9.376002060220186E-4 62-63 0.0 0.0 0.0 0.8755935790637625 9.376002060220186E-4 64-65 0.0 0.0 0.0 0.9744791474588846 9.376002060220186E-4 66-67 0.0 0.0 0.0 1.090616559644812 9.376002060220186E-4 68-69 0.0 0.0 0.0 1.2240995756421467 9.376002060220186E-4 70-71 0.0 0.0 0.0 1.3800537432438091 9.376002060220186E-4 72-73 0.0 0.0 0.0 1.541133458638392 0.0010001068864234864 74-75 0.0 0.0 0.0 1.696306292735036 0.0010001068864234864 76-77 0.0 0.0 0.0 1.8638554495511708 0.0010313602266242205 78-79 0.0 0.0 0.0 2.0656895205675108 0.0010626135668249544 80-81 0.0 0.0 0.0 2.292713783785642 0.0010938669070256883 82-83 0.0 0.0 0.0 2.529082795723793 0.0011876269276278903 84-85 0.0 0.0 0.0 2.7698272752900466 0.0011876269276278903 86-87 0.0 0.0 0.0 3.0318865328732008 0.0011876269276278903 88-89 0.0 0.0 0.0 3.3264180109249177 0.0011876269276278903 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTACGC 30 0.0039595184 31.654367 78-79 TACCTGG 1195 0.0 29.376606 2 TACATGG 11745 0.0 28.112148 2 GTACATG 12025 0.0 27.528982 1 CCGTACG 35 0.008409689 27.132315 78-79 ACATGGG 11895 0.0 26.837011 3 CATGGGA 7650 0.0 23.809664 4 GTACGAT 100 0.0016675061 23.71666 3 TAGACTC 820 0.0 22.560455 5 ATGGGAT 2610 0.0 22.172588 5 CATGGGT 1175 0.0 21.799143 4 CATGGGG 4060 0.0 21.613705 4 ATGGGAG 2820 0.0 21.194273 5 AGTACAT 8495 0.0 20.382963 2 ACACCGA 165 6.3918786E-5 20.123856 6 GTACCTG 1775 0.0 20.096893 1 CTAGACT 1105 0.0 19.745998 4 AGTACTT 5985 0.0 19.73478 12-13 GAGTACT 5735 0.0 19.685238 12-13 GCGTGTA 170 8.040748E-5 19.531977 9 >>END_MODULE