FastQCFastQC Report
Thu 26 May 2016
SRR936320_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936320_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3240053
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53750.040.6114161
GGTATCA31750.036.8365441
TCAACGC65550.032.569334
ATCAACG66400.032.15293
CAACGCA66600.032.0558475
TATCAAC70400.030.5272852
AACGCAG71400.029.9657466
TACATGG117050.027.5006182
GTACATG120600.027.0126251
ACATGGG121250.025.6114273
ACGCAGA86400.024.4350197
CGCAGAG88700.023.8021538
CATGGGG85250.023.2106274
GTACCTG31500.022.848551
GAGTACA84750.022.5718571
TACCTGG30250.022.377712
AGTACAT80850.021.3121032
GTCTACG2401.4629222E-719.7294251
GTGTAGC16350.019.1140121
GCAGAGT108000.019.0226489
Produced by FastQC (version 0.11.5)