FastQCFastQC Report
Thu 26 May 2016
SRR936320_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936320_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3240053
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34500.037.2292671
GTATCAA55700.036.638861
TCAACGC68600.029.1113874
ATCAACG69500.028.7344063
CAACGCA71300.028.0755215
TATCAAC74150.027.2611482
AACGCAG74750.027.0335666
TACATGG119550.026.4741442
GTACATG121850.026.4303531
ACATGGG123750.024.724043
ACGCAGA91000.021.9455097
CGCAGAG92100.021.5288878
TACCTGG29250.021.4138012
CATGGGG81550.021.2893334
GTACCTG30850.021.1254141
GAGTACA89150.019.689961
TCGCGTA1951.0457612E-519.4607819
CGTACAC4001.8189894E-1218.9742623
AGTACAT84750.018.8684352
ACCGTGC6950.017.7457128
Produced by FastQC (version 0.11.5)