FastQCFastQC Report
Thu 26 May 2016
SRR936319_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936319_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2288807
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45200.19748279343780403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33090.144573133514534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG107950.030.3942031
GTATCAA87200.029.7539221
TACATGG108950.029.5444852
ACATGGG111000.027.973523
GGTATCA60900.026.277231
TCAACGC103600.024.9910434
ATCAACG103700.024.967493
TACCTGG22550.024.770242
GTACCTG23450.024.4299561
TATCAAC106300.024.3138752
CAACGCA106600.024.2877315
AACGCAG108250.024.0039256
CATGGGG54850.023.2993564
CATGGGA61950.021.8515154
ACGCAGA124500.020.6427867
TATACAG10250.020.3182185
CGCAGAG127200.020.1310758
AGTACAT74650.020.0378912
GAGTACT54400.019.66589712-13
GTACTTT54000.019.2417714-15
Produced by FastQC (version 0.11.5)