FastQCFastQC Report
Thu 26 May 2016
SRR936319_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936319_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2288807
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44660.19512348572859134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33340.14566540560213248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA82900.029.3812541
GTACATG112150.028.4204161
TACATGG113900.027.7835872
GGTATCA58300.026.6829661
ACATGGG114800.026.3224833
TACCTGG23450.026.0995542
GTACCTG24950.025.1685541
ATCAACG100100.024.4063263
TCAACGC100500.024.4035954
TATCAAC103600.023.8596572
CAACGCA102400.023.8581395
TATAACG1000.001665430623.722272
AACGCAG104350.023.5941436
CATGGGG54750.022.8743654
AGTACAT79600.020.9208952
ACGCAGA120600.020.2969917
CATGGGA67900.019.8416524
CGCAGAG123000.019.7466838
GAGTACA99850.019.2957571
CCGTATG1559.712883E-418.363229
Produced by FastQC (version 0.11.5)