##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936319_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2288807 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.449281219430034 34.0 31.0 34.0 31.0 34.0 2 32.57612939841586 34.0 31.0 34.0 31.0 34.0 3 32.66287546306875 34.0 31.0 34.0 31.0 34.0 4 36.16259212768923 37.0 35.0 37.0 35.0 37.0 5 36.119488449659585 37.0 35.0 37.0 35.0 37.0 6 36.155671491742204 37.0 36.0 37.0 35.0 37.0 7 36.09951647299226 37.0 36.0 37.0 35.0 37.0 8 36.07246788392381 37.0 36.0 37.0 35.0 37.0 9 37.83480739092462 39.0 38.0 39.0 35.0 39.0 10-11 37.765152326080795 39.0 38.0 39.0 35.0 39.0 12-13 37.7087248946722 39.0 37.0 39.0 35.0 39.0 14-15 39.04429949751115 40.0 38.0 41.0 35.5 41.0 16-17 38.973604371185516 40.0 38.0 41.0 35.0 41.0 18-19 38.98472850703445 40.0 38.0 41.0 35.0 41.0 20-21 38.933479319138755 40.0 38.0 41.0 35.0 41.0 22-23 38.82031403259427 40.0 38.0 41.0 35.0 41.0 24-25 38.969751927532556 40.0 38.0 41.0 35.0 41.0 26-27 38.89672086812038 40.0 38.0 41.0 35.0 41.0 28-29 38.80166086524552 40.0 38.0 41.0 35.0 41.0 30-31 38.691570979990885 40.0 38.0 41.0 35.0 41.0 32-33 38.55481501935287 40.0 38.0 41.0 34.0 41.0 34-35 38.4243887754625 40.0 38.0 41.0 34.0 41.0 36-37 38.28489558097297 40.0 38.0 41.0 34.0 41.0 38-39 38.15196541254899 40.0 38.0 41.0 33.0 41.0 40-41 38.008159709403195 40.0 37.5 41.0 33.0 41.0 42-43 38.04747123719912 40.0 38.0 41.0 33.0 41.0 44-45 37.91986349220358 40.0 37.5 41.0 33.0 41.0 46-47 37.712291163038216 40.0 37.0 41.0 33.0 41.0 48-49 37.484787271272765 40.0 37.0 41.0 32.5 41.0 50-51 37.280446756760185 40.0 36.5 41.0 32.0 41.0 52-53 37.05498213698228 40.0 36.0 41.0 31.5 41.0 54-55 36.84258939263992 39.0 35.5 41.0 31.0 41.0 56-57 36.580555503369226 39.0 35.0 41.0 31.0 41.0 58-59 36.29910167174428 39.0 35.0 41.0 31.0 41.0 60-61 35.99940951770944 38.0 35.0 40.0 30.0 41.0 62-63 35.68765343692151 37.5 35.0 40.0 30.0 41.0 64-65 35.29502269086035 37.0 34.5 40.0 29.0 41.0 66-67 34.93707136512602 36.5 34.0 39.0 29.0 41.0 68-69 34.56787575361312 36.0 34.0 39.0 29.0 41.0 70-71 34.18296802657454 35.5 34.0 38.5 28.0 40.0 72-73 33.78566519588589 35.0 34.0 37.5 28.0 40.0 74-75 33.36157832442841 35.0 33.0 37.0 27.0 39.0 76-77 32.65422576040706 35.0 32.5 36.0 26.0 39.0 78-79 32.71935488662871 35.0 33.0 36.0 26.0 38.5 80-81 32.56815624908522 35.0 33.0 36.0 26.0 37.0 82-83 32.30985137672158 35.0 33.0 35.0 26.0 37.0 84-85 32.04373566665953 35.0 33.0 35.0 26.0 36.5 86-87 31.651405950785716 35.0 32.0 35.0 25.0 36.0 88-89 31.175434844440794 34.5 32.0 35.0 23.5 36.0 90-91 30.977221102521966 34.0 32.0 35.0 23.0 35.0 92-93 30.69981086216531 34.0 31.0 35.0 20.0 35.0 94-95 30.554745550848107 34.0 31.0 35.0 20.0 35.0 96-97 30.304540312922846 34.0 31.0 35.0 18.5 35.0 98-99 29.845744092883322 34.0 31.0 35.0 3.5 35.0 100-101 26.483473923314634 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.026998280069420844 1101 2 0.12447667870304002 1101 3 0.23646925743931746 1101 4 0.1372650159710247 1101 5 0.11185281639254185 1101 6 0.14649782871120465 1101 7 0.09765511632856771 1101 8 -0.029037117095128906 1101 9 0.177947405786 1101 10-11 0.11112888131471976 1101 12-13 0.28369154405901753 1101 14-15 0.38839008876474423 1101 16-17 0.42249103425267975 1101 18-19 0.37691325747567817 1101 20-21 0.1754312642596858 1101 22-23 0.19069716310445273 1101 24-25 0.23549866238943906 1101 26-27 0.33386116496571816 1101 28-29 0.2338691472569394 1101 30-31 0.1908761862974515 1101 32-33 0.24743515429067742 1101 34-35 0.3081028548774114 1101 36-37 0.19417602342847573 1101 38-39 0.2030294505709449 1101 40-41 0.23618631904864884 1101 42-43 0.2267569246427925 1101 44-45 0.2724097098232079 1101 46-47 0.3099384201106332 1101 48-49 0.20630877200589737 1101 50-51 0.24251502202073283 1101 52-53 0.26081014568244854 1101 54-55 0.24912221849533012 1101 56-57 0.17772342109621775 1101 58-59 0.1065572040714784 1101 60-61 0.27016934990788855 1101 62-63 0.21500584034731673 1101 64-65 0.337275949529932 1101 66-67 0.25478787116307444 1101 68-69 0.24799593990773872 1101 70-71 0.15417796092845037 1101 72-73 0.26101850403560434 1101 74-75 0.1977968194905344 1101 76-77 -0.025830766155884533 1101 78-79 0.006252611146258857 1101 80-81 0.29037968829295124 1101 82-83 0.16375199866205037 1101 84-85 0.3119513685238431 1101 86-87 0.2543162126738885 1101 88-89 0.2994697256850145 1101 90-91 0.39521180062401484 1101 92-93 0.4715257261717376 1101 94-95 0.45060698131554844 1101 96-97 0.35995195399638646 1101 98-99 0.33971119032895203 1101 100-101 0.7016240209036795 1102 1 0.0030694944385558642 1102 2 0.1050657124477894 1102 3 0.12132042067047877 1102 4 0.09364242687414759 1102 5 0.06167320213703675 1102 6 0.13120567933780336 1102 7 0.12683911834883332 1102 8 0.14656691080979556 1102 9 0.12705817317133494 1102 10-11 0.11375080751188449 1102 12-13 0.1863810228935776 1102 14-15 0.20508821686222234 1102 16-17 0.2124313732785339 1102 18-19 0.34446778886871243 1102 20-21 0.2213828410140053 1102 22-23 0.3029412364653581 1102 24-25 0.3007545101119078 1102 26-27 0.22860657990936772 1102 28-29 0.21237099390614844 1102 30-31 0.2691229743905197 1102 32-33 0.28015083344721603 1102 34-35 0.31447426811647006 1102 36-37 0.20241239452604276 1102 38-39 0.28541083887234464 1102 40-41 0.19007122703184365 1102 42-43 0.24442414898700093 1102 44-45 0.22471691649008818 1102 46-47 0.27982193916110276 1102 48-49 0.3142648312249463 1102 50-51 0.12706251405015223 1102 52-53 0.13135908977790933 1102 54-55 0.049952421468901775 1102 56-57 0.15412734798784555 1102 58-59 0.2119626777291046 1102 60-61 0.27849347630076693 1102 62-63 0.36318349747033096 1102 64-65 0.3211655925110861 1102 66-67 0.3320668256755255 1102 68-69 0.23164820165842315 1102 70-71 0.19583647287642947 1102 72-73 0.11412119035777835 1102 74-75 0.1640665881069694 1102 76-77 0.09545462795377802 1102 78-79 0.2507376012027649 1102 80-81 0.23347258895268652 1102 82-83 0.1745048633358124 1102 84-85 0.2812856278123306 1102 86-87 0.161984384017682 1102 88-89 0.26086497174707546 1102 90-91 0.418716961771775 1102 92-93 0.2934626559990683 1102 94-95 0.36225760160027676 1102 96-97 0.2627209807964732 1102 98-99 0.3849714573828358 1102 100-101 0.6079468214971335 1103 1 0.1252236606798789 1103 2 0.1097307068817841 1103 3 0.1650551384569141 1103 4 0.08497987273318586 1103 5 0.13693436696376438 1103 6 0.11394619646603843 1103 7 0.1314525846829966 1103 8 0.131336954511454 1103 9 0.20930649972337534 1103 10-11 0.23835899950752548 1103 12-13 0.21957873037924713 1103 14-15 0.2931243400046597 1103 16-17 0.22278244491717203 1103 18-19 0.1708038736081221 1103 20-21 0.18020277461081236 1103 22-23 0.22567606605803547 1103 24-25 0.2328755962853606 1103 26-27 0.2178161826082743 1103 28-29 0.2807016293059519 1103 30-31 0.16949266420107278 1103 32-33 0.17910848318252448 1103 34-35 0.17378918744581995 1103 36-37 0.1611606262858274 1103 38-39 0.13048966228165426 1103 40-41 0.2416853690646832 1103 42-43 0.27642480037996364 1103 44-45 0.17724155032460942 1103 46-47 0.38628616359028456 1103 48-49 0.43482235554279924 1103 50-51 0.3552889751877544 1103 52-53 0.31876496592574455 1103 54-55 0.3883306474439294 1103 56-57 0.346040839113833 1103 58-59 0.30841756876661464 1103 60-61 0.316410744533691 1103 62-63 0.4106102522208843 1103 64-65 0.4151967184000753 1103 66-67 0.3339254831254692 1103 68-69 0.2523031918632199 1103 70-71 0.1879653125705616 1103 72-73 0.38407338103119315 1103 74-75 0.30450666724635056 1103 76-77 0.3233999935845375 1103 78-79 0.16461543099475762 1103 80-81 0.4265622332629704 1103 82-83 0.43561816196059056 1103 84-85 0.4273212453960511 1103 86-87 0.31914484543723276 1103 88-89 0.39886876829858053 1103 90-91 0.6173096613094806 1103 92-93 0.7370103413665454 1103 94-95 0.7017226572846553 1103 96-97 0.5312238221336756 1103 98-99 0.524108629073492 1103 100-101 0.9993440593965381 1104 1 0.05747722214734097 1104 2 0.06716759042047471 1104 3 0.02109760633209845 1104 4 0.05149387528592797 1104 5 0.056945601262860635 1104 6 0.01229942618798674 1104 7 0.0626223421722969 1104 8 0.042210588112020275 1104 9 0.0926373303663155 1104 10-11 0.15476323127890623 1104 12-13 0.20875459443310973 1104 14-15 0.21562748922340091 1104 16-17 0.14184891359322194 1104 18-19 0.21242118165872625 1104 20-21 0.19129768775355416 1104 22-23 0.2567031701949887 1104 24-25 0.2687568769954538 1104 26-27 0.3196982753023647 1104 28-29 0.26003357781726777 1104 30-31 0.21497280535281504 1104 32-33 0.18352886008769786 1104 34-35 0.2640730481516371 1104 36-37 0.24644710091271094 1104 38-39 0.24560924917090432 1104 40-41 0.36167407915024086 1104 42-43 0.41274704123332384 1104 44-45 0.2990878128345855 1104 46-47 0.34986402367876934 1104 48-49 0.42786279067723854 1104 50-51 0.5007376936712333 1104 52-53 0.40060537492097126 1104 54-55 0.3178979878904613 1104 56-57 0.45300165532459147 1104 58-59 0.5292115194858553 1104 60-61 0.4781627529427297 1104 62-63 0.41754687575785 1104 64-65 0.218103940886472 1104 66-67 0.24570356122609383 1104 68-69 0.39731463471984796 1104 70-71 0.42649117231423617 1104 72-73 0.33410890533092186 1104 74-75 0.42421104389568853 1104 76-77 0.40423237450514193 1104 78-79 0.28380258496305544 1104 80-81 0.4072977739692263 1104 82-83 0.4957161005779369 1104 84-85 0.38012293777091344 1104 86-87 0.42638721431711346 1104 88-89 0.6365037397818121 1104 90-91 0.8296921391398193 1104 92-93 0.7752330279768742 1104 94-95 0.7504170176108929 1104 96-97 0.7731655066667713 1104 98-99 0.7833883964901283 1104 100-101 0.7784774611367418 1105 1 -0.025072414938939858 1105 2 0.020258861650255255 1105 3 0.08895910697359 1105 4 0.026538959128416195 1105 5 0.04167249635432313 1105 6 0.055132889614000646 1105 7 0.09560352807922357 1105 8 0.04537296172025407 1105 9 0.05530947886935422 1105 10-11 0.1018483702089128 1105 12-13 0.06791951157752862 1105 14-15 0.08166152416001893 1105 16-17 0.10260746606543591 1105 18-19 0.05462223765240992 1105 20-21 0.10108805910086005 1105 22-23 -0.047279908784723546 1105 24-25 0.08898146630632198 1105 26-27 0.14738098822819268 1105 28-29 0.029544451770917135 1105 30-31 0.011171743975985748 1105 32-33 0.05366574898855703 1105 34-35 0.04854828119697174 1105 36-37 0.04825651065082326 1105 38-39 0.07889940519239502 1105 40-41 0.14236854976275737 1105 42-43 0.04195038057783762 1105 44-45 -0.12095487102226343 1105 46-47 -0.10756337114802506 1105 48-49 -0.08075057836429522 1105 50-51 -0.0556363990444666 1105 52-53 -0.1262127824283681 1105 54-55 -0.14004663748747959 1105 56-57 0.02532611992592848 1105 58-59 -0.051516595785223274 1105 60-61 0.0062154449640132725 1105 62-63 -9.697985349390592E-4 1105 64-65 0.06622746612892882 1105 66-67 0.02832441283968734 1105 68-69 0.011632792069192988 1105 70-71 -0.017069352183561648 1105 72-73 -0.08554944437614154 1105 74-75 -0.1284409402419655 1105 76-77 -0.10003365605317782 1105 78-79 -0.04433074208994725 1105 80-81 0.06939986980716384 1105 82-83 -0.06555418715117867 1105 84-85 -0.03966494387166364 1105 86-87 -0.09750251194994419 1105 88-89 0.02025011737357474 1105 90-91 0.1837501335592897 1105 92-93 0.10289544195869738 1105 94-95 1.6872003061862983E-4 1105 96-97 0.003269962116757341 1105 98-99 -0.043806244588651566 1105 100-101 0.13709094232713426 1106 1 -0.009811799543555821 1106 2 0.05989200478744294 1106 3 0.044832142294062294 1106 4 0.03749915795923897 1106 5 0.008924045344421927 1106 6 0.043396445465155864 1106 7 0.06605369785553705 1106 8 0.05605224200869685 1106 9 0.02802537483322709 1106 10-11 0.127656557978014 1106 12-13 0.13240869490093843 1106 14-15 0.11933217561099241 1106 16-17 0.046351558899814904 1106 18-19 0.14327654156952008 1106 20-21 0.19080784066700573 1106 22-23 0.10871564552618906 1106 24-25 0.2136012198681243 1106 26-27 0.10706130117669943 1106 28-29 0.07260156312317889 1106 30-31 0.13493386460468315 1106 32-33 0.14828060179073788 1106 34-35 0.3013189606522886 1106 36-37 0.11698091293317958 1106 38-39 0.2244366509654938 1106 40-41 0.1549958819992554 1106 42-43 0.1324700361244453 1106 44-45 0.3174088878603669 1106 46-47 0.2900571350304304 1106 48-49 0.22276352882231976 1106 50-51 0.1813092025237708 1106 52-53 0.10002728478204403 1106 54-55 0.056570963240631045 1106 56-57 0.08210448307222151 1106 58-59 0.10520707138318386 1106 60-61 0.057435384054578265 1106 62-63 0.10607591855120546 1106 64-65 0.027682116599756057 1106 66-67 0.06482063225369927 1106 68-69 0.05573131484021587 1106 70-71 -0.07017914670171166 1106 72-73 0.009970736251858625 1106 74-75 0.0802740980889709 1106 76-77 0.12138747672239703 1106 78-79 -0.06012300239029145 1106 80-81 -0.0338298182944996 1106 82-83 0.07717606103729224 1106 84-85 0.12419239151532224 1106 86-87 0.19601098264171668 1106 88-89 0.1545584976725216 1106 90-91 0.3158308560712797 1106 92-93 0.39855914483264243 1106 94-95 0.3175375371972784 1106 96-97 0.2890319427237493 1106 98-99 0.2002117657029423 1106 100-101 0.3402151765834418 1107 1 0.023098627099791713 1107 2 0.09369663636409342 1107 3 0.03614395526215475 1107 4 0.0018121692665715727 1107 5 -0.016756765122352135 1107 6 0.047130678023044936 1107 7 0.011418254128805927 1107 8 0.028303227203700487 1107 9 0.02973576071302375 1107 10-11 0.012002603231024978 1107 12-13 0.008411405495188262 1107 14-15 0.08853237742697218 1107 16-17 0.1019441044471705 1107 18-19 0.11687725074614264 1107 20-21 0.14315247284418575 1107 22-23 0.03518235999298014 1107 24-25 0.1505163243110772 1107 26-27 0.25581198473961564 1107 28-29 0.24760720450764495 1107 30-31 0.1244650018622977 1107 32-33 0.14581795549007381 1107 34-35 0.19037487951098342 1107 36-37 0.24589894543665025 1107 38-39 0.22768499653850682 1107 40-41 0.17019827576607582 1107 42-43 -0.05545562680647009 1107 44-45 -0.09085560390093406 1107 46-47 0.02523109894922726 1107 48-49 -0.03598448418198075 1107 50-51 -0.040824291740669594 1107 52-53 0.12569228572379387 1107 54-55 0.1720342937866377 1107 56-57 0.06085488911541148 1107 58-59 0.011619005277076155 1107 60-61 0.12240331915317171 1107 62-63 0.1362770020609858 1107 64-65 0.1796941118209574 1107 66-67 0.14637583001606913 1107 68-69 0.20435131391212025 1107 70-71 0.36780588725122954 1107 72-73 0.1877495275579406 1107 74-75 0.06406683271369928 1107 76-77 -0.0016957036717926144 1107 78-79 0.05399356135144018 1107 80-81 0.19189482663949065 1107 82-83 0.31728816990509756 1107 84-85 0.2067195907226207 1107 86-87 0.3104381637419884 1107 88-89 0.42067682025234276 1107 90-91 0.08457230869138144 1107 92-93 0.40242235581769137 1107 94-95 0.49866988134720813 1107 96-97 0.31078277824008893 1107 98-99 0.25659524245179455 1107 100-101 0.3665633992288697 1108 1 -0.1653639193813916 1108 2 -0.11850191170171342 1108 3 -0.08814731211396065 1108 4 -0.13805456521352966 1108 5 -0.07964875751410005 1108 6 -0.14907246128946383 1108 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-0.6689866452048534 2316 80-81 -0.6442049140908139 2316 82-83 -0.7952987222759589 2316 84-85 -0.7314922328212035 2316 86-87 -0.8585884016814909 2316 88-89 -0.6805683018144997 2316 90-91 -0.8050931235850634 2316 92-93 -0.904938420586241 2316 94-95 -0.7960098036002101 2316 96-97 -0.6639207771175037 2316 98-99 -0.8631179852137336 2316 100-101 -0.8044414532957198 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 12.0 5 33.0 6 69.0 7 164.0 8 376.0 9 705.0 10 1197.0 11 1783.0 12 2812.0 13 4021.0 14 4892.0 15 6763.0 16 8729.0 17 11781.0 18 10938.0 19 7490.0 20 6916.0 21 7386.0 22 8090.0 23 9235.0 24 11154.0 25 13520.0 26 17048.0 27 21141.0 28 27194.0 29 34025.0 30 42367.0 31 52490.0 32 66310.0 33 86512.0 34 119820.0 35 179895.0 36 311943.0 37 558393.0 38 564429.0 39 89143.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.7310678310178 17.642075049656064 12.503646993781931 24.1232101255442 2 16.658356577548425 19.00250089255164 38.48022817858724 25.858914351312695 3 20.435886468365396 22.95623877417362 27.975796998174157 28.632077759286823 4 13.307893588231773 14.498863381665645 34.64791919982768 37.545323830274896 5 14.579516752613916 37.03042676818098 33.09038289379576 15.299673585409343 6 35.71441366615883 33.65508756308417 15.987062255576815 14.643436515180177 7 31.619441918868652 30.908722316910076 18.813032291495087 18.658803472726184 8 28.51267057467056 34.36148176757586 18.1221483506473 19.003699307106277 9 27.59437558518477 15.34380137774832 18.61913215050461 38.4426908865623 10-11 26.827092799720376 25.493599265990913 25.777219503670047 21.902088430618665 12-13 27.041769167366997 22.19959275700797 26.86870946439092 23.88992861123411 14-15 23.539643141601715 23.679060750862785 24.642532113891647 28.13876399364385 16-17 24.045326093415216 27.463002317379647 25.015663284387575 23.476008304817565 18-19 23.916935766099982 25.925252762683794 26.886801726838478 23.27100974437775 20-21 24.427392366891812 25.17695365552031 26.92164100444533 23.474012973142543 22-23 24.193072720012093 25.038819366953774 26.837333526738394 23.930774386295735 24-25 24.01472239207413 25.26577181471964 26.959746954517044 23.759758838689184 26-27 23.85158860072454 25.637971136766446 26.845786986356586 23.664653276152432 28-29 23.576168344247165 25.704720345601608 27.062877865221928 23.6562334449293 30-31 23.65805720557301 25.491566360339053 27.044773406624184 23.80560302746375 32-33 23.59638828473235 25.559054760321466 26.970640176793342 23.873916778152847 34-35 24.03471036177089 25.529138152430413 26.697036704009886 23.739114781788814 36-37 24.072677179132445 25.40804563426397 26.64213859930929 23.877138587294294 38-39 23.879485504107276 25.479360722753174 26.875526885553956 23.76562688758559 40-41 23.942811841424888 25.370942926093658 26.957104730413878 23.729140502067573 42-43 23.899700685467636 25.473368521706035 26.67659065870388 23.950340134122445 44-45 23.86582893495054 25.58130745569576 26.63067129225069 23.922192317103008 46-47 24.021663088633126 25.77543116651467 26.2969297369763 23.9059760078759 48-49 24.155724642462467 25.707941573982268 26.14501807078083 23.991315712774433 50-51 24.030245487339382 25.859952846017254 26.089998916402173 24.019802750241187 52-53 24.320329871753923 25.743528818555244 26.079731824086462 23.856409485604377 54-55 23.973407881908347 25.639303765709386 26.13176738799855 24.255520964383713 56-57 23.937323524432138 26.129296173108536 26.07693105915379 23.856449243305534 58-59 24.21102462867153 25.623439258240925 26.088120044976016 24.07741606811153 60-61 24.133954363013125 25.611925482555222 26.405710991382087 23.848409163049563 62-63 24.021946804601697 25.715995276141673 26.372079428278578 23.889978490978052 64-65 23.94915476025854 25.7472457590555 26.18931369150098 24.11428578918498 66-67 23.96506127427957 26.000837991145602 25.949370130377964 24.084730604196857 68-69 23.77948424659659 25.754530635392147 26.454524125450508 24.011460992560753 70-71 23.74316973912352 25.89320140413976 26.23991094036688 24.12371791636984 72-73 23.952603363378582 25.82107420142697 26.068390445523143 24.157931989671308 74-75 23.725460469143968 26.10150178673868 26.03271049066173 24.14032725345562 76-77 23.97017845257057 25.923961707914394 25.961652319647033 24.144207519868008 78-79 23.949942770869605 25.884667885163857 26.108532848033803 24.05685649593273 80-81 23.78071771704201 26.221204700310423 26.061338220933816 23.936739361713748 82-83 23.883861767287502 26.18567227380902 25.884467322932863 24.045998635970616 84-85 23.862768746940255 25.972329963362796 25.723093848352857 24.441807441344096 86-87 23.85881996807509 25.840041225045262 26.1379647933564 24.16317401352325 88-89 23.791415224967484 25.951134698767696 25.949003107508457 24.308446968756368 90-91 23.75894062839814 26.12186560272513 26.011591095465025 24.107602673411705 92-93 23.73669351790685 25.929643396687364 26.068336461048176 24.265326624357613 94-95 23.733861071903796 25.873164043666446 26.062830105129002 24.330144779300753 96-97 23.785840618260494 25.787899389359403 25.971624611577354 24.45463538080275 98-99 23.639459014627857 25.823380846178228 25.810295097825254 24.726865041368658 100-101 23.608490246597437 25.866494581637205 25.908175975875476 24.616839195889884 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 154.0 1 103.5 2 119.5 3 215.5 4 187.5 5 166.0 6 370.5 7 746.5 8 1116.5 9 1238.5 10 1083.0 11 938.5 12 969.5 13 1094.5 14 1241.5 15 1383.0 16 1456.5 17 1526.5 18 1606.5 19 1716.5 20 1919.5 21 2162.5 22 2486.0 23 2954.0 24 3580.0 25 4500.0 26 5757.5 27 7264.5 28 9104.0 29 11293.5 30 14262.0 31 17922.0 32 22162.0 33 25912.5 34 29257.0 35 32070.0 36 36224.0 37 41223.5 38 45004.5 39 48082.5 40 50710.5 41 55947.0 42 61517.0 43 66611.0 44 72104.5 45 77476.0 46 82796.5 47 87780.5 48 96171.0 49 98693.0 50 96037.5 51 95300.5 52 94387.0 53 96116.0 54 98410.0 55 95402.5 56 88139.5 57 81061.5 58 75160.5 59 68091.5 60 58178.0 61 48035.5 62 39454.5 63 31094.5 64 24242.5 65 19214.0 66 13969.0 67 10952.0 68 7863.5 69 4756.0 70 3348.5 71 2406.5 72 1835.0 73 1577.0 74 1239.5 75 966.5 76 651.5 77 319.0 78 158.5 79 60.5 80 28.5 81 18.5 82 8.5 83 5.0 84 4.5 85 4.0 86 2.5 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2667328437915473 2 0.018524934605670115 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.0583618452757267E-4 12-13 6.553632525590843E-5 14-15 0.0 16-17 6.553632525590843E-4 18-19 0.0 20-21 6.553632525590843E-5 22-23 4.369088350393895E-5 24-25 0.002119007849941039 26-27 0.0020753169664371003 28-29 0.0019442443159252833 30-31 0.0011578084128543822 32-33 0.0014417991556299854 34-35 0.00166025357314968 36-37 2.1845441751969475E-5 38-39 1.5291809226378633E-4 40-41 5.024451602952979E-4 42-43 0.003386043471555269 44-45 0.0038011068648426886 46-47 0.013369410352205319 48-49 0.013369410352205319 50-51 0.005898269273031759 52-53 0.0069905413606302326 54-55 3.713725097834811E-4 56-57 0.003036516403523757 58-59 0.0013762628303740768 60-61 0.001201499296358321 62-63 0.0 64-65 8.082813448228707E-4 66-67 0.0 68-69 0.0 70-71 1.5291809226378633E-4 72-73 0.0016165626896457413 74-75 0.0 76-77 0.19304816876215425 78-79 0.10415906627339047 80-81 9.611994370866569E-4 82-83 0.0 84-85 5.898269273031759E-4 86-87 0.12312090971409997 88-89 0.5903293724634712 90-91 0.3157977059664707 92-93 0.29663925354999354 94-95 0.0020753169664371003 96-97 0.0027525256607481537 98-99 0.0025122258014764897 100-101 6.990541360630232E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2288807.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.10215094263544 #Duplication Level Percentage of deduplicated Percentage of total 1 68.03175875416923 30.003468934693313 2 15.400610964506575 13.584001387309508 3 6.1332682265668685 8.11470963299166 4 3.1714552394806437 5.594719907175494 5 1.8874139236364167 4.1619506875722525 6 1.168829046305014 3.092872501576821 7 0.8408411075351905 2.595803101030281 8 0.5704035250797328 2.0124817889022175 9 0.41796840310327676 1.6589975042621707 >10 2.152786871766691 16.824236645180545 >50 0.14446641269044203 4.409116639737911 >100 0.07564059747125625 5.9492099551657684 >500 0.0031700366529754842 0.9526866608774223 >1k 0.0013868910356766963 1.0457446535247643 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4466 0.19512348572859134 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3334 0.14566540560213248 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.1845441751969475E-5 0.0 12-13 0.0 0.0 0.0 4.369088350393895E-5 0.0 14-15 0.0 0.0 0.0 4.369088350393895E-5 0.0 16-17 0.0 0.0 0.0 1.0922720875984738E-4 0.0 18-19 0.0 0.0 0.0 2.6214530102363373E-4 0.0 20-21 0.0 0.0 0.0 6.116723690551453E-4 0.0 22-23 0.0 0.0 0.0 0.0015073354808858939 0.0 24-25 0.0 0.0 0.0 0.0021190078499410394 0.0 26-27 0.0 0.0 0.0 0.0025122258014764897 0.0 28-29 0.0 0.0 0.0 0.00332050714629936 0.0 30-31 0.0 0.0 0.0 0.005723505739016003 0.0 32-33 0.0 0.0 0.0 0.0095901489291146 0.0 34-35 0.0 0.0 0.0 0.014483527881555762 0.0 36-37 0.0 0.0 0.0 0.023571231650375064 0.0 38-39 0.0 0.0 0.0 0.03951840412931278 0.0 40-41 0.0 0.0 0.0 0.05904822905557349 0.0 42-43 0.0 0.0 0.0 0.07822852691380269 0.0 44-45 0.0 0.0 0.0 0.10024873219978792 0.0 46-47 0.0 0.0 0.0 0.128101670433549 0.0 48-49 0.0 0.0 0.0 0.15905666139608976 0.0 50-51 0.0 0.0 0.0 0.19289525066989047 0.0 52-53 0.0 0.0 0.0 0.22533573167156515 0.0 54-55 0.0 0.0 0.0 0.2593927753628856 0.0 56-57 0.0 0.0 0.0 0.30271228635704106 0.0 58-59 0.0 0.0 0.0 0.3547481286102323 0.0 60-61 0.0 0.0 0.0 0.4149323206369082 0.0 62-63 0.0 0.0 0.0 0.4728008958378753 0.0 64-65 0.0 0.0 0.0 0.5308005436893544 0.0 66-67 0.0 0.0 0.0 0.5980408134019164 0.0 68-69 0.0 0.0 0.0 0.6795024656950106 0.0 70-71 0.0 0.0 0.0 0.7706416486842271 0.0 72-73 0.0 0.0 0.0 0.8616279135811801 0.0 74-75 0.0 0.0 0.0 0.9555851585564008 0.0 76-77 0.0 0.0 0.0 1.063589022578138 0.0 78-79 0.0 0.0 0.0 1.1917780747786948 0.0 80-81 0.0 0.0 0.0 1.3373997894973233 0.0 82-83 0.0 0.0 0.0 1.488526555537448 0.0 84-85 0.0 0.0 0.0 1.6371410957760966 0.0 86-87 0.0 0.0 0.0 1.7983386104638792 0.0 88-89 0.0 0.0 0.0 1.9828670569427653 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8290 0.0 29.381254 1 GTACATG 11215 0.0 28.420416 1 TACATGG 11390 0.0 27.783587 2 GGTATCA 5830 0.0 26.682966 1 ACATGGG 11480 0.0 26.322483 3 TACCTGG 2345 0.0 26.099554 2 GTACCTG 2495 0.0 25.168554 1 ATCAACG 10010 0.0 24.406326 3 TCAACGC 10050 0.0 24.403595 4 TATCAAC 10360 0.0 23.859657 2 CAACGCA 10240 0.0 23.858139 5 TATAACG 100 0.0016654306 23.72227 2 AACGCAG 10435 0.0 23.594143 6 CATGGGG 5475 0.0 22.874365 4 AGTACAT 7960 0.0 20.920895 2 ACGCAGA 12060 0.0 20.296991 7 CATGGGA 6790 0.0 19.841652 4 CGCAGAG 12300 0.0 19.746683 8 GAGTACA 9985 0.0 19.295757 1 CCGTATG 155 9.712883E-4 18.36322 9 >>END_MODULE