FastQCFastQC Report
Thu 26 May 2016
SRR936318_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936318_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3558227
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT137100.38530425405686597No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT54770.15392497443249123No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT52830.14847282087399144No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC47060.13225687962010294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG134900.030.1138291
TACATGG140400.028.4540372
ACATGGG136900.027.9363
AGTACAT91050.023.8616942
GAGTACA104700.023.1081331
CATGGGG86000.022.7301254
TACCTGG32650.020.8760262
CATGGGA62950.020.7521744
GTATACG1856.744309E-620.4742931
GTACCTG35250.020.416211
GTATAGG8150.019.7520391
GTATAGC8300.017.6837441
GTACTAG6050.016.4344271
GTCCTAA11000.015.9257411
CTTACAC12350.015.71351053
AGAGTAC102450.015.66342710-11
GTGTAGG17350.015.5548571
TACACTG27250.015.4587265
ATGGGAG33300.015.3507575
ATGGGGA47550.015.3292335
Produced by FastQC (version 0.11.5)