##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936318_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3558227 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.36889917366149 33.0 31.0 34.0 28.0 34.0 2 31.455331826777776 33.0 31.0 34.0 28.0 34.0 3 31.52802505292664 34.0 31.0 34.0 28.0 34.0 4 35.02212028631113 37.0 35.0 37.0 32.0 37.0 5 34.9844270756194 37.0 35.0 37.0 32.0 37.0 6 35.07224834166004 37.0 35.0 37.0 32.0 37.0 7 35.00147432977154 37.0 35.0 37.0 32.0 37.0 8 34.99851976841275 37.0 35.0 37.0 32.0 37.0 9 36.620949141243656 39.0 37.0 39.0 32.0 39.0 10-11 36.51947318144683 39.0 37.0 39.0 32.0 39.0 12-13 36.40892908181519 39.0 37.0 39.0 32.0 39.0 14-15 37.58802768907099 40.0 38.0 41.0 32.0 41.0 16-17 37.52385808999819 40.0 38.0 41.0 32.0 41.0 18-19 37.431400807199765 40.0 37.0 41.0 32.0 41.0 20-21 37.325650106078115 40.0 37.0 41.0 31.5 41.0 22-23 37.148780136849055 40.0 37.0 41.0 31.0 41.0 24-25 37.31141211620282 40.0 37.0 41.0 31.5 41.0 26-27 37.26013334731033 40.0 37.0 41.0 31.0 41.0 28-29 37.12201427845947 40.0 37.0 41.0 31.0 41.0 30-31 36.98590196746863 40.0 37.0 41.0 30.5 41.0 32-33 36.817177628071505 40.0 36.5 41.0 30.0 41.0 34-35 36.55039448017229 40.0 36.0 41.0 30.0 41.0 36-37 36.35594651493567 40.0 36.0 41.0 30.0 41.0 38-39 36.18929525856557 40.0 36.0 41.0 29.0 41.0 40-41 36.017558744846795 39.0 35.0 41.0 28.5 41.0 42-43 35.836305412780014 39.0 35.0 41.0 28.0 41.0 44-45 35.66630192508797 39.0 35.0 41.0 27.0 41.0 46-47 35.42606092865913 39.0 35.0 41.0 26.5 41.0 48-49 35.280797992933 39.0 35.0 40.5 26.0 41.0 50-51 34.97670974898453 38.5 34.5 40.5 26.0 41.0 52-53 35.01542762729865 38.5 34.5 40.0 26.0 41.0 54-55 35.08686208046873 39.0 35.0 40.5 26.0 41.0 56-57 34.89125089546002 38.0 34.0 40.5 26.0 41.0 58-59 34.62837643579232 38.0 34.0 40.0 26.0 41.0 60-61 34.273704572530086 37.5 34.0 40.0 25.0 41.0 62-63 33.95326942322679 37.0 34.0 40.0 24.0 41.0 64-65 33.59206143958775 36.5 33.0 39.5 23.0 41.0 66-67 33.19179495855661 36.0 33.0 39.0 22.5 41.0 68-69 32.78060477872828 35.0 33.0 39.0 22.0 40.5 70-71 32.3424525753978 35.0 32.0 37.5 20.5 40.0 72-73 31.924159138807052 35.0 32.0 37.0 19.5 39.5 74-75 31.509738276956472 35.0 31.5 36.5 18.0 39.0 76-77 31.121408639752325 35.0 31.0 36.0 17.0 39.0 78-79 30.733384491770764 35.0 31.0 36.0 12.0 37.5 80-81 30.335455691837538 34.5 31.0 35.0 7.5 37.0 82-83 29.994075560665465 34.0 30.0 35.0 7.0 36.5 84-85 29.687463587904872 34.0 30.0 35.0 2.0 36.0 86-87 29.380232205533822 34.0 30.0 35.0 2.0 36.0 88-89 29.045778838730637 34.0 29.5 35.0 2.0 35.5 90-91 28.667650208938326 34.0 29.0 35.0 2.0 35.0 92-93 28.32488315107496 34.0 29.0 35.0 2.0 35.0 94-95 27.916357781558062 34.0 28.5 35.0 2.0 35.0 96-97 27.439060380352352 34.0 27.0 35.0 2.0 35.0 98-99 26.872870393035633 33.5 26.5 35.0 2.0 35.0 100-101 23.50606875278053 29.0 13.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.499581662389339 1101 2 0.43868961251282457 1101 3 0.5673506788601692 1101 4 0.28469215288928496 1101 5 0.6061495142818742 1101 6 0.4906493322174086 1101 7 0.5874350570150924 1101 8 0.4804561658252524 1101 9 0.5590662097113608 1101 10-11 0.7715483482825292 1101 12-13 0.7143809953874367 1101 14-15 0.8891281319291338 1101 16-17 0.8384359598264481 1101 18-19 0.8255425513094394 1101 20-21 0.9044637261503397 1101 22-23 0.86129950029801 1101 24-25 0.8356303322682805 1101 26-27 0.7956146683319858 1101 28-29 0.8074710013110291 1101 30-31 0.8751800260308968 1101 32-33 1.00485966546821 1101 34-35 0.7751073594683646 1101 36-37 0.6978261334330824 1101 38-39 0.7391479606174087 1101 40-41 0.6917001462027272 1101 42-43 0.5721658221417272 1101 44-45 0.7175045938624365 1101 46-47 0.5964599951984297 1101 48-49 0.6928286063482147 1101 50-51 0.8066487472835249 1101 52-53 0.771412873417681 1101 54-55 0.8305778089773312 1101 56-57 0.7293280515828116 1101 58-59 0.7562744319113364 1101 60-61 0.7156838534117611 1101 62-63 0.6636581502841281 1101 64-65 0.6972766986595644 1101 66-67 0.7017127298068644 1101 68-69 0.7209341239078597 1101 70-71 0.8623107001197639 1101 72-73 0.8196548521484743 1101 74-75 0.6346197755911582 1101 76-77 0.721774188107279 1101 78-79 0.839491647435409 1101 80-81 0.8624981819226782 1101 82-83 0.9681520560838273 1101 84-85 0.86726063565429 1101 86-87 0.8817603891811778 1101 88-89 0.7902827101310059 1101 90-91 0.7857782475500414 1101 92-93 0.8851576414604061 1101 94-95 1.1942835499599056 1101 96-97 1.3376988992804542 1101 98-99 1.4090308837437853 1101 100-101 1.4451011614985667 1102 1 0.5063463274156703 1102 2 0.5643919994741786 1102 3 0.4937772471879178 1102 4 0.4857760671938962 1102 5 0.5825072266326643 1102 6 0.4724314298205883 1102 7 0.5059738877975732 1102 8 0.5139752436168834 1102 9 0.484428354426214 1102 10-11 0.6545457455285728 1102 12-13 0.6688897307342998 1102 14-15 0.8296921320320862 1102 16-17 0.7749214049755793 1102 18-19 0.7943027743442528 1102 20-21 0.7188331109105448 1102 22-23 0.8796737506579078 1102 24-25 0.8498386928712236 1102 26-27 0.756523011192904 1102 28-29 0.7734596460183099 1102 30-31 0.7850913276652633 1102 32-33 0.604696460396859 1102 34-35 0.5905019703314025 1102 36-37 0.5498117181803579 1102 38-39 0.6815530157012049 1102 40-41 0.6227277555927628 1102 42-43 0.6233815933335833 1102 44-45 0.633049481495263 1102 46-47 0.6450668724654278 1102 48-49 0.6555661663454586 1102 50-51 0.49734679501175094 1102 52-53 0.5118097781700826 1102 54-55 0.512576465455659 1102 56-57 0.6820440166009547 1102 58-59 0.7097999384875777 1102 60-61 0.6221012226045559 1102 62-63 0.7542305302056533 1102 64-65 0.6186016963165528 1102 66-67 0.6745750361254821 1102 68-69 0.6694937283882822 1102 70-71 0.7853679035316574 1102 72-73 0.7327793672143628 1102 74-75 0.8687447840151883 1102 76-77 0.854601041800759 1102 78-79 0.773531767216717 1102 80-81 0.6695791384362337 1102 82-83 0.7427066436759198 1102 84-85 0.7769849386410215 1102 86-87 0.6856329951122042 1102 88-89 0.8090591524864017 1102 90-91 0.7836362853678764 1102 92-93 0.8640145143557518 1102 94-95 0.8730687474082757 1102 96-97 1.019442133387436 1102 98-99 0.9914700750451217 1102 100-101 1.0475875705724285 1103 1 0.23337392243448107 1103 2 0.17084110365016514 1103 3 0.2563056378009314 1103 4 0.18068321877480997 1103 5 0.17191961300186875 1103 6 0.202352879460463 1103 7 0.28059680664657805 1103 8 0.2682026741166581 1103 9 0.15186767484133412 1103 10-11 0.28375107885371875 1103 12-13 0.28611817959848196 1103 14-15 0.33470050521867023 1103 16-17 0.42572192458410285 1103 18-19 0.36432067557355197 1103 20-21 0.37381394116194144 1103 22-23 0.5277525889647166 1103 24-25 0.5727988251080092 1103 26-27 0.5575648468156302 1103 28-29 0.5229958091776155 1103 30-31 0.4368297842115467 1103 32-33 0.5094640882830319 1103 34-35 0.30619677216470365 1103 36-37 0.3240764301986587 1103 38-39 0.37036902054929755 1103 40-41 0.4678497017824199 1103 42-43 0.3688938406116833 1103 44-45 0.5414538990693742 1103 46-47 0.6379377460248961 1103 48-49 0.5652796286971977 1103 50-51 0.4160438199431198 1103 52-53 0.4923266645359803 1103 54-55 0.5666833458563971 1103 56-57 0.5115393876325385 1103 58-59 0.5280604968605189 1103 60-61 0.49646189127393825 1103 62-63 0.5225838463548982 1103 64-65 0.44413223712973604 1103 66-67 0.5859132582858493 1103 68-69 0.5393473407811555 1103 70-71 0.4873439928300485 1103 72-73 0.4463730790418907 1103 74-75 0.5437921029025254 1103 76-77 0.45172218879926973 1103 78-79 0.5419006221279332 1103 80-81 0.615780539399502 1103 82-83 0.7241600282053184 1103 84-85 0.7763182586097415 1103 86-87 0.738687124389525 1103 88-89 0.5553418154196734 1103 90-91 0.6865577260873579 1103 92-93 0.6816390865482163 1103 94-95 0.6566485155948847 1103 96-97 0.8194695372411083 1103 98-99 0.8581883105784129 1103 100-101 0.9896882730683458 1104 1 0.271205672179498 1104 2 0.1304805733615133 1104 3 0.26314006180499305 1104 4 0.2572012883475381 1104 5 0.2729153232439927 1104 6 0.19632211735284244 1104 7 0.26124482140846794 1104 8 0.26082572491606015 1104 9 0.2675710202059065 1104 10-11 0.37789996318873875 1104 12-13 0.41887934393559334 1104 14-15 0.536864994956531 1104 16-17 0.5589547328292213 1104 18-19 0.37559042885557403 1104 20-21 0.44112891614865646 1104 22-23 0.515950111240997 1104 24-25 0.4288033481331368 1104 26-27 0.45770333008796626 1104 28-29 0.4570038431954302 1104 30-31 0.460192879325227 1104 32-33 0.5538655023043546 1104 34-35 0.6159673722598171 1104 36-37 0.6700568712363406 1104 38-39 0.5857438594755209 1104 40-41 0.651722870910838 1104 42-43 0.6467691149979871 1104 44-45 0.6734216667556225 1104 46-47 0.8526745140072123 1104 48-49 0.7400582547279839 1104 50-51 0.7290225183044043 1104 52-53 0.7540061218844443 1104 54-55 0.8731212712660579 1104 56-57 0.9552695846499972 1104 58-59 0.8685644990521766 1104 60-61 0.8305193656872305 1104 62-63 0.8891513070870829 1104 64-65 0.8736325742106743 1104 66-67 0.9635614710136835 1104 68-69 0.8541375066201482 1104 70-71 0.8055362747216748 1104 72-73 0.9128913780730414 1104 74-75 0.8342399806767951 1104 76-77 0.8350221391823673 1104 78-79 0.7894358251851799 1104 80-81 0.9025881102643005 1104 82-83 0.8962533403095669 1104 84-85 0.6741594170794016 1104 86-87 0.6098632623477371 1104 88-89 0.7648774017746796 1104 90-91 0.8699244145014795 1104 92-93 0.7369401476215955 1104 94-95 0.5393084906220054 1104 96-97 0.8187765170414245 1104 98-99 0.9613359135692505 1104 100-101 1.2512904856050078 1105 1 0.3845579689730698 1105 2 0.42949111391808614 1105 3 0.4056762060259729 1105 4 0.32705145451816264 1105 5 0.28992384555435535 1105 6 0.29222944564001807 1105 7 0.4133133967925744 1105 8 0.34459627539153104 1105 9 0.2958657400232312 1105 10-11 0.4177005548037087 1105 12-13 0.41981146312857476 1105 14-15 0.5415145709271059 1105 16-17 0.596999426661128 1105 18-19 0.5641933239486718 1105 20-21 0.5912990140696905 1105 22-23 0.6597709237746585 1105 24-25 0.638340604511896 1105 26-27 0.6286433056752756 1105 28-29 0.623922155741333 1105 30-31 0.7018687021106516 1105 32-33 0.6256603120049249 1105 34-35 0.4578125401600843 1105 36-37 0.46360335393531216 1105 38-39 0.5341835280448848 1105 40-41 0.5399316039343702 1105 42-43 0.5317563363395124 1105 44-45 0.5207098048699876 1105 46-47 0.6050503885095182 1105 48-49 0.6082130015701424 1105 50-51 0.5321838780501693 1105 52-53 0.5324115858425316 1105 54-55 0.682723114788665 1105 56-57 0.7703914929973905 1105 58-59 0.767088989833205 1105 60-61 0.6607134518231348 1105 62-63 0.5878925062837794 1105 64-65 0.651224327227844 1105 66-67 0.5963501808072564 1105 68-69 0.6387869991471433 1105 70-71 0.6895788603334267 1105 72-73 0.6757999636372674 1105 74-75 0.5642391444270167 1105 76-77 0.6828189203281774 1105 78-79 0.7157669361983352 1105 80-81 0.7538545570389985 1105 82-83 0.7003502658309095 1105 84-85 0.6472090912231572 1105 86-87 0.5497510978521447 1105 88-89 0.6386292173527615 1105 90-91 0.6648017315800026 1105 92-93 0.6553379057465989 1105 94-95 0.6671041906292778 1105 96-97 0.7284730599558245 1105 98-99 0.7211371781748248 1105 100-101 0.7039339872458505 1106 1 0.08819135565019565 1106 2 0.11680345341736142 1106 3 0.11718801939518642 1106 4 0.10459600767686084 1106 5 0.2545909534209869 1106 6 0.0974623808909243 1106 7 0.1421452745160039 1106 8 0.04610359193779345 1106 9 0.03991314404667179 1106 10-11 0.17174934150678212 1106 12-13 0.1100043851968664 1106 14-15 0.17323309045269042 1106 16-17 0.17978772559681744 1106 18-19 0.16704627072013523 1106 20-21 0.1276335481573696 1106 22-23 0.11797164650503333 1106 24-25 0.16660608184867698 1106 26-27 0.15428569560766192 1106 28-29 0.21633680552567114 1106 30-31 0.27990682029257385 1106 32-33 0.23709490351740214 1106 34-35 0.10825017516932434 1106 36-37 0.35323326558440016 1106 38-39 0.3409482659060856 1106 40-41 0.1631812107070516 1106 42-43 0.26705499958904255 1106 44-45 0.3715027013489234 1106 46-47 0.39408537421915923 1106 48-49 0.3867266316415936 1106 50-51 0.3285300714904267 1106 52-53 0.24662198234771182 1106 54-55 0.1952423713163114 1106 56-57 0.28650233550128235 1106 58-59 0.2818199109451953 1106 60-61 0.3206803653850159 1106 62-63 0.3466206937117846 1106 64-65 0.19660326775357362 1106 66-67 0.27951817389136835 1106 68-69 0.39185434133970887 1106 70-71 0.41502997958322396 1106 72-73 0.4521394323074759 1106 74-75 0.3915371197178068 1106 76-77 0.39919560390078956 1106 78-79 0.48806086577207 1106 80-81 0.48929313429902876 1106 82-83 0.524250577298627 1106 84-85 0.37826691410243285 1106 86-87 0.4731380561402396 1106 88-89 0.3553551298545905 1106 90-91 0.4088037135987612 1106 92-93 0.30276941372333965 1106 94-95 0.3266541648886587 1106 96-97 0.49366549710751784 1106 98-99 0.48231570702860793 1106 100-101 0.6432089933273311 1107 1 0.05520463256171482 1107 2 0.11924026621495187 1107 3 0.22893770098760768 1107 4 0.12108753758189295 1107 5 0.09423185689167468 1107 6 0.2492420793866259 1107 7 0.18317518326099247 1107 8 0.18613680505148977 1107 9 0.2773779319277381 1107 10-11 0.3115481505932891 1107 12-13 0.23045870315350214 1107 14-15 0.4089303922924543 1107 16-17 0.26742932618939363 1107 18-19 0.3344830958812963 1107 20-21 0.39973367178386354 1107 22-23 0.3241794197803429 1107 24-25 0.36038238247049037 1107 26-27 0.37320609815433414 1107 28-29 0.48776989194487896 1107 30-31 0.3143373329839463 1107 32-33 0.40286718076054484 1107 34-35 0.309022742269363 1107 36-37 0.3376134585677377 1107 38-39 0.3105049112259124 1107 40-41 0.4466619039934798 1107 42-43 0.02844621565186145 1107 44-45 0.2225836569627191 1107 46-47 0.38218123049262687 1107 48-49 0.323256944675137 1107 50-51 0.2866441461632476 1107 52-53 0.29132565563603663 1107 54-55 0.46232829103868056 1107 56-57 0.45390298578814736 1107 58-59 0.5020257120945644 1107 60-61 0.5708720235322744 1107 62-63 0.5940639772327785 1107 64-65 0.2218896880446195 1107 66-67 0.2316120168270288 1107 68-69 0.3913640573156556 1107 70-71 0.5158261381962248 1107 72-73 0.42775564381637565 1107 74-75 0.38803291917382765 1107 76-77 0.36363991524611805 1107 78-79 0.38497703558370944 1107 80-81 0.4247312408749693 1107 82-83 0.5706770904226453 1107 84-85 0.5209072366687479 1107 86-87 0.3713650843395193 1107 88-89 0.5164080513145421 1107 90-91 0.7595501661028479 1107 92-93 0.5183885155589074 1107 94-95 0.7043679787726873 1107 96-97 0.8000194260331419 1107 98-99 0.7464291052770662 1107 100-101 0.9791400031713842 1108 1 0.004712973367031026 1108 2 0.07298407692866249 1108 3 0.08382281670727565 1108 4 0.11879759159955938 1108 5 0.14324956518716903 1108 6 0.044791567263935406 1108 7 0.06896605791049382 1108 8 0.12862885175389493 1108 9 0.14484950197107338 1108 10-11 0.013551423348708624 1108 12-13 0.053422870448024184 1108 14-15 0.011519682723353242 1108 16-17 0.1286235080979381 1108 18-19 0.1477785243607599 1108 20-21 0.22621309394807554 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-2.0704432486995543 2316 44-45 -2.3150664406153965 2316 46-47 -2.407923095560257 2316 48-49 -2.5140205567298253 2316 50-51 -2.3931445618027567 2316 52-53 -2.585578788447471 2316 54-55 -2.49949227585563 2316 56-57 -2.431652148507439 2316 58-59 -2.7127613446393752 2316 60-61 -2.6971130165825272 2316 62-63 -2.6458871118580163 2316 64-65 -2.375512354054429 2316 66-67 -2.538192552598339 2316 68-69 -2.6543528889659633 2316 70-71 -2.5093057159308856 2316 72-73 -2.4905734552705354 2316 74-75 -2.4425300582916343 2316 76-77 -2.962376008380712 2316 78-79 -2.9281081649260656 2316 80-81 -2.676177425634382 2316 82-83 -2.7183080753404774 2316 84-85 -2.9022029198458696 2316 86-87 -2.9187691214981264 2316 88-89 -2.974635638257336 2316 90-91 -3.0860464657048006 2316 92-93 -3.2658039911018406 2316 94-95 -3.232953499546266 2316 96-97 -3.4906319299253568 2316 98-99 -3.422533051227628 2316 100-101 -3.251391146303572 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42210.0 3 9559.0 4 7717.0 5 6442.0 6 6188.0 7 6538.0 8 7568.0 9 8917.0 10 10299.0 11 12119.0 12 14604.0 13 18594.0 14 17561.0 15 15316.0 16 16906.0 17 19031.0 18 18349.0 19 17100.0 20 17845.0 21 19375.0 22 20655.0 23 23087.0 24 26536.0 25 31186.0 26 37160.0 27 44488.0 28 53603.0 29 63223.0 30 77304.0 31 95570.0 32 120502.0 33 156770.0 34 216648.0 35 318473.0 36 510515.0 37 773990.0 38 611603.0 39 84650.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.48260061853345 16.987569440207263 11.487519812443606 25.042310128815682 2 16.881366546394258 19.163141271312234 37.09500114819534 26.86049103409817 3 21.08338797792705 22.006645044927552 27.431904591523114 29.478062385622284 4 13.345311952818516 13.81720547969846 34.81649553148589 38.02098703599713 5 14.956158114662616 36.69061432041775 33.04720004699402 15.306027517925608 6 36.28298089351708 33.19594368420298 15.831559634368341 14.689515787911603 7 32.06792833365748 30.25766404237834 18.930878876146558 18.74352874781762 8 28.60727252899337 33.210320072447594 18.56146437683993 19.6209430217191 9 27.403846829523598 15.322191437517057 18.550191234013823 38.72377049894552 10-11 27.09950395570732 24.73733541306578 26.024591430940237 22.138569200286664 12-13 27.364424751152015 21.616995442529923 26.63743589959794 24.381143906720126 14-15 23.881722530534123 23.542590372705284 24.23472521048201 28.34096188627858 16-17 24.619039065730398 26.659886656901428 24.657844221123888 24.063230056244286 18-19 24.507747947386306 26.075031037604617 25.73545139512522 23.681769619883855 20-21 25.033968293448954 25.020140679112924 25.558138864941167 24.38775216249695 22-23 24.574973393457398 24.978544017473396 25.687128982250734 24.759353606818472 24-25 24.681787496832317 25.123822510784176 25.636020920273328 24.558369072110175 26-27 24.62953732198841 25.095873339405184 26.06927107874915 24.205318259857258 28-29 24.23334586061437 25.837545245081685 25.73365407670238 24.19545481760157 30-31 24.420489046713495 25.31222273863293 25.56307466403986 24.704213550613723 32-33 24.358872558437085 25.38578953219273 25.79063963843303 24.464698270937156 34-35 24.89981166297493 25.178660923329065 25.67280080559271 24.248726608103297 36-37 24.73639194540514 25.097234677949487 25.792405445029015 24.373967931616363 38-39 24.470767815499975 25.459136608864995 25.833671458013384 24.236424117621645 40-41 24.582381804759045 25.49405585300924 25.669877999115453 24.253684343116262 42-43 24.440273192232116 25.522288611149506 25.306484199640828 24.730953996977554 44-45 24.530342096515035 25.204180975645713 25.549615899411656 24.715861028427597 46-47 24.753449016331093 25.528800682748965 25.426269707025504 24.291480593894434 48-49 24.9147001107578 25.45480327567393 25.302536017470413 24.327960596097856 50-51 24.08535254665881 25.59104550852309 25.87487786973459 24.448724075083504 52-53 24.69721775915719 25.752294806348175 25.187378113129093 24.36310932136554 54-55 24.428922961775683 25.274920652853144 25.43363612934203 24.862520256029146 56-57 24.45290039924238 25.45294798693471 25.509474257670835 24.584677356152078 58-59 25.062247847325196 25.248670141974372 25.23518481464594 24.453897196054488 60-61 24.70388724113123 25.352134950293102 25.849479279555325 24.094498529020342 62-63 24.493424726586245 25.462485959547966 25.6995022037597 24.34458711010609 64-65 24.338819883539262 25.795066327374343 25.410375605863173 24.455738183223218 66-67 24.4457976115111 25.552790551762733 25.229550533307204 24.77186130341896 68-69 24.10411777690125 25.493656881723027 25.53106695244628 24.871158388929445 70-71 24.52477063650215 25.29388334354421 25.493803793306625 24.687542226647015 72-73 24.474612545020804 25.284344522790065 25.6956708011578 24.545372131031336 74-75 24.14915629187876 25.88912378382554 25.422659064977548 24.539060859318145 76-77 24.4909365524783 25.361272655794597 25.308332881460093 24.83945791026701 78-79 24.39224737041212 25.506723529337837 25.54398899800229 24.557040102247747 80-81 24.556318593448356 25.47002473632311 25.472284148150198 24.501372522078338 82-83 24.4054491433994 25.42260939803544 25.732361031874696 24.439580426690465 84-85 24.344123016023755 25.543705197831716 25.376326563580935 24.735845222563594 86-87 24.292103088063698 25.504059034962616 25.587574030592698 24.61626384638099 88-89 24.236520201513752 25.526756218919527 25.265288309575297 24.971435269991428 90-91 24.369682012044805 25.31649754117004 25.50735529916353 24.806465147621623 92-93 24.47564903718141 25.521603543591652 25.537311153773405 24.465436265453526 94-95 24.36967575012914 25.317170876171396 25.566213665608796 24.74693970809067 96-97 24.059951989618643 25.359400225990996 25.885456107093308 24.69519167729705 98-99 24.249445029317528 25.5424427292559 25.270497919442224 24.93761432198435 100-101 24.170364812335134 25.30950426377902 25.232579069260204 25.28755185462564 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 75.0 1 64.0 2 122.0 3 219.5 4 194.5 5 164.0 6 327.5 7 604.5 8 862.0 9 964.0 10 912.5 11 956.0 12 1133.5 13 1368.5 14 1664.5 15 1967.0 16 2330.0 17 2563.0 18 2530.5 19 2579.5 20 2605.5 21 2489.5 22 2522.0 23 2661.0 24 3012.0 25 3723.0 26 5003.0 27 6813.5 28 8994.0 29 12004.5 30 15864.0 31 20789.5 32 26632.0 33 31495.5 34 37058.5 35 42786.5 36 48331.0 37 55543.0 38 63289.5 39 69238.5 40 74924.5 41 84000.5 42 92749.0 43 100169.0 44 108227.5 45 116635.0 46 124906.5 47 139197.5 48 165493.5 49 169900.0 50 153792.0 51 148504.0 52 150448.5 53 154572.0 54 156430.0 55 151665.5 56 141684.0 57 131893.5 58 124934.5 59 115129.5 60 99149.0 61 81980.5 62 67048.0 63 53820.0 64 41266.0 65 31788.5 66 24297.0 67 18702.0 68 13666.0 69 9305.5 70 6845.0 71 5251.0 72 4214.0 73 3767.5 74 3018.0 75 2243.0 76 1512.0 77 821.0 78 521.5 79 326.0 80 173.5 81 117.0 82 89.0 83 60.5 84 47.5 85 42.0 86 45.5 87 41.0 88 39.5 89 41.0 90 33.5 91 31.5 92 33.5 93 32.5 94 31.0 95 27.5 96 32.5 97 32.0 98 22.5 99 18.5 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04918179756378668 2 0.013208825631416996 3 0.010904307116999561 4 0.006210958435198205 5 0.009218074057669733 6 0.00618285455087604 7 0.0051430108309559784 8 0.009695840091146518 9 0.015288513071257118 10-11 0.002163999092806614 12-13 0.009330489594958387 14-15 0.00904945075173675 16-17 0.01995375786873631 18-19 0.012534332407685062 20-21 0.0036394530197202146 22-23 0.01015955418246222 24-25 0.02294682154904676 26-27 0.00779882789940046 28-29 0.018492355883983794 30-31 0.015471188319351181 32-33 0.013784955260021353 34-35 0.2679845889539931 36-37 0.4720047371907414 38-39 0.4674800118148729 40-41 0.4899350153882818 42-43 0.4793820068253093 44-45 0.49513423398788214 46-47 0.5011906210593084 48-49 0.5078371897015002 50-51 0.5188398604136274 52-53 0.5075280469739564 54-55 0.4950358703927546 56-57 0.4888811197262007 58-59 0.48736350997280387 60-61 0.4839348360854999 62-63 0.4811244476532835 64-65 0.49801488213090395 66-67 0.4704871274373445 68-69 0.49846454428005854 70-71 0.47969114955285314 72-73 0.4879958473700525 74-75 0.502314776432195 76-77 0.48931672993319425 78-79 0.48944319741264397 80-81 0.4913261576622289 82-83 0.49145262514167876 84-85 0.4904830411325641 86-87 0.4930967023745253 88-89 0.5064882032540363 90-91 0.532343776830427 92-93 0.5210881711594004 94-95 0.49097485910820193 96-97 0.46351736412544786 98-99 0.45382152403430137 100-101 0.46299744226548784 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3558227.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.18879685510998 #Duplication Level Percentage of deduplicated Percentage of total 1 65.07393773150429 24.85095388590493 2 14.279651584254935 10.906454270257221 3 6.594281579685692 7.554830389560317 4 3.832892689116981 5.854942410884982 5 2.4438708591074616 4.666424388928897 6 1.6085836402699192 3.6857924317632746 7 1.1263854151658563 3.0110712660229315 8 0.8531704107004122 2.606524119762303 9 0.6429093754891707 2.20967419831214 >10 3.281757916306701 21.75253308075335 >50 0.17514111228790447 4.598389558420202 >100 0.08263148241673175 5.559651037698893 >500 0.0027980883169900763 0.7167481730153464 >1k 0.0017672136684653802 1.3379167971280796 >5k 1.4726780570544835E-4 0.30257014096761464 >10k+ 7.363390285272417E-5 0.38552385061951644 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13710 0.38530425405686597 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5477 0.15392497443249123 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5283 0.14847282087399144 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4706 0.13225687962010294 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 5.6207768644327636E-5 0.0 0.0 0.0 0.0 7 1.1241553728865527E-4 0.0 0.0 0.0 0.0 8 1.1241553728865527E-4 0.0 0.0 0.0 0.0 9 1.1241553728865527E-4 0.0 0.0 0.0 0.0 10-11 1.405194216108191E-4 0.0 0.0 2.8103884322163818E-5 2.8103884322163818E-5 12-13 1.9672719025514674E-4 0.0 0.0 2.8103884322163818E-5 2.8103884322163818E-5 14-15 1.9672719025514674E-4 0.0 0.0 4.2155826483245725E-5 2.8103884322163818E-5 16-17 1.9672719025514674E-4 0.0 0.0 7.025971080540955E-5 2.8103884322163818E-5 18-19 2.1077913241622864E-4 0.0 0.0 8.431165296649146E-5 5.6207768644327636E-5 20-21 2.2483107457731054E-4 0.0 0.0 1.264674794497372E-4 5.6207768644327636E-5 22-23 2.810388432216382E-4 0.0 0.0 2.669869010605563E-4 5.6207768644327636E-5 24-25 4.215582648324573E-4 0.0 0.0 3.372466118659658E-4 5.6207768644327636E-5 26-27 4.496621491546211E-4 0.0 0.0 5.058699177989488E-4 5.6207768644327636E-5 28-29 6.885451658930136E-4 0.0 0.0 0.00154571363771901 5.6207768644327636E-5 30-31 7.588048766984231E-4 0.0 0.0 0.004890075872056504 5.6207768644327636E-5 32-33 7.588048766984231E-4 0.0 0.0 0.01052490467865035 5.6207768644327636E-5 34-35 8.150126453427508E-4 0.0 0.0 0.019237108818521136 5.6207768644327636E-5 36-37 8.993242983092422E-4 0.0 0.0 0.033682505360113336 5.6207768644327636E-5 38-39 9.836359512757336E-4 1.4051942161081909E-5 0.0 0.05696657352102606 5.6207768644327636E-5 40-41 9.836359512757336E-4 2.8103884322163818E-5 0.0 0.08471915928916283 5.6207768644327636E-5 42-43 0.0010679476042422251 2.8103884322163818E-5 0.0 0.11148810910602387 5.6207768644327636E-5 44-45 0.0011522592572087167 2.8103884322163818E-5 0.0 0.14418697851486145 5.6207768644327636E-5 46-47 0.0013068306209806175 2.8103884322163818E-5 0.0 0.18306870247457513 5.6207768644327636E-5 48-49 0.0013208825631416995 2.8103884322163818E-5 0.0 0.22582876247074737 5.6207768644327636E-5 50-51 0.0013208825631416995 2.8103884322163818E-5 0.0 0.2723828468504117 5.6207768644327636E-5 52-53 0.0013208825631416995 2.8103884322163818E-5 0.0 0.3193584894949086 5.6207768644327636E-5 54-55 0.0013489864474638634 2.8103884322163818E-5 0.0 0.37222189590489874 5.6207768644327636E-5 56-57 0.0013770903317860271 2.8103884322163818E-5 0.0 0.4351324409600624 5.6207768644327636E-5 58-59 0.0013770903317860271 2.8103884322163818E-5 0.0 0.5037199706483032 5.6207768644327636E-5 60-61 0.0013770903317860271 2.8103884322163818E-5 0.0 0.5758766936454588 5.6207768644327636E-5 62-63 0.0013770903317860271 2.8103884322163818E-5 0.0 0.6503941429256761 5.6207768644327636E-5 64-65 0.0013770903317860271 2.8103884322163818E-5 0.0 0.7299562394417219 5.6207768644327636E-5 66-67 0.0015176097533968463 2.8103884322163818E-5 0.0 0.8249473684506357 5.6207768644327636E-5 68-69 0.0015176097533968463 2.8103884322163818E-5 0.0 0.9311097914776095 5.6207768644327636E-5 70-71 0.0016300252906855015 2.8103884322163818E-5 0.0 1.0503124168300673 5.6207768644327636E-5 72-73 0.0016721811171687474 2.8103884322163818E-5 0.0 1.1694166785873976 5.6207768644327636E-5 74-75 0.001714336943651993 2.8103884322163818E-5 0.0 1.2940855094405164 5.6207768644327636E-5 76-77 0.001714336943651993 2.8103884322163818E-5 0.0 1.43180859456128 5.6207768644327636E-5 78-79 0.001714336943651993 2.8103884322163818E-5 0.0 1.5967502916480596 5.6207768644327636E-5 80-81 0.0020796874398401224 2.8103884322163818E-5 0.0 1.780395123751239 8.431165296649146E-5 82-83 0.0020796874398401224 2.8103884322163818E-5 0.0 1.9635481378787807 8.431165296649146E-5 84-85 0.0021499471506455318 2.8103884322163818E-5 0.0 2.150930786596808 8.431165296649146E-5 86-87 0.0022202068614509416 2.8103884322163818E-5 0.0 2.3521545983435006 8.431165296649146E-5 88-89 0.0022202068614509416 2.8103884322163818E-5 0.0 2.5838289687532585 8.431165296649146E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13490 0.0 30.113829 1 TACATGG 14040 0.0 28.454037 2 ACATGGG 13690 0.0 27.936 3 AGTACAT 9105 0.0 23.861694 2 GAGTACA 10470 0.0 23.108133 1 CATGGGG 8600 0.0 22.730125 4 TACCTGG 3265 0.0 20.876026 2 CATGGGA 6295 0.0 20.752174 4 GTATACG 185 6.744309E-6 20.474293 1 GTACCTG 3525 0.0 20.41621 1 GTATAGG 815 0.0 19.752039 1 GTATAGC 830 0.0 17.683744 1 GTACTAG 605 0.0 16.434427 1 GTCCTAA 1100 0.0 15.925741 1 CTTACAC 1235 0.0 15.7135105 3 AGAGTAC 10245 0.0 15.663427 10-11 GTGTAGG 1735 0.0 15.554857 1 TACACTG 2725 0.0 15.458726 5 ATGGGAG 3330 0.0 15.350757 5 ATGGGGA 4755 0.0 15.329233 5 >>END_MODULE