FastQCFastQC Report
Thu 26 May 2016
SRR936318_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936318_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3558227
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT136750.3843206181055902No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT57280.16097904939735436No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT54870.15420601327571287No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC49480.13905801962606656No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT38010.10682286430854467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG126350.029.4444311
TACATGG133100.027.4131322
ACATGGG132450.026.252143
CATGGGG78500.020.9083214
AGTACAT91750.020.737132
CATGGGA64100.020.2030684
GAGTACA105000.020.071791
GTATAGG9350.018.3173481
GTGTAGC17950.018.2875841
TAGCCCT17200.016.5476194
TACCTGG31650.016.4903282
TATACAG15300.015.5023445
TAGCGCG4601.9281288E-1015.4684274
GTACCTG35250.015.3857051
ATGGGAG33150.015.3115455
ACTATAC7450.015.2815593
GCACCGT14100.015.1395236
TACCGTA2205.762576E-415.0936467
ATGGGGA42750.015.0911245
AGAGTAC106950.014.9918325
Produced by FastQC (version 0.11.5)