##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936318_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3558227 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46658827556533 34.0 31.0 34.0 31.0 34.0 2 32.605148856438895 34.0 31.0 34.0 31.0 34.0 3 32.69526705294519 34.0 31.0 34.0 31.0 34.0 4 36.180823483156075 37.0 35.0 37.0 35.0 37.0 5 36.13584630772573 37.0 35.0 37.0 35.0 37.0 6 36.16856344465938 37.0 37.0 37.0 35.0 37.0 7 36.11384068526263 37.0 36.0 37.0 35.0 37.0 8 36.089268335044395 37.0 36.0 37.0 35.0 37.0 9 37.857764274173626 39.0 38.0 39.0 35.0 39.0 10-11 37.790146609533345 39.0 38.0 39.0 35.0 39.0 12-13 37.7398044025859 39.0 38.0 39.0 35.0 39.0 14-15 39.104273701481105 40.5 38.0 41.0 36.0 41.0 16-17 39.03422758019654 40.0 38.0 41.0 36.0 41.0 18-19 39.02948898426098 40.0 38.5 41.0 35.0 41.0 20-21 38.97010379045519 40.0 38.0 41.0 35.0 41.0 22-23 38.85788146737124 40.0 38.0 41.0 35.0 41.0 24-25 39.00479747357322 40.0 38.0 41.0 35.0 41.0 26-27 38.934057326865315 40.0 38.0 41.0 35.0 41.0 28-29 38.8533224552565 40.0 38.0 41.0 35.0 41.0 30-31 38.774447779750986 40.0 38.0 41.0 35.0 41.0 32-33 38.65851728965015 40.0 38.0 41.0 34.5 41.0 34-35 38.53621663260944 40.0 38.0 41.0 34.0 41.0 36-37 38.41315309562881 40.0 38.0 41.0 34.0 41.0 38-39 38.29414691642776 40.0 38.0 41.0 34.0 41.0 40-41 38.191080136258876 40.0 38.0 41.0 33.0 41.0 42-43 38.24048100360095 40.0 38.0 41.0 34.0 41.0 44-45 38.15112807586475 40.0 38.0 41.0 33.0 41.0 46-47 38.0169182573231 40.0 37.5 41.0 33.0 41.0 48-49 37.844205274143555 40.0 37.0 41.0 33.0 41.0 50-51 37.65418324912941 40.0 37.0 41.0 33.0 41.0 52-53 37.45123301576881 40.0 36.0 41.0 32.5 41.0 54-55 37.250594045854854 39.5 36.0 41.0 32.0 41.0 56-57 36.971925062678686 39.0 35.5 41.0 31.5 41.0 58-59 36.69470806668602 39.0 35.0 41.0 31.0 41.0 60-61 36.37277680710084 38.0 35.0 40.0 31.0 41.0 62-63 36.06486151670481 37.5 35.0 40.0 31.0 41.0 64-65 35.67156058340291 37.0 35.0 40.0 30.0 41.0 66-67 35.29328145731006 36.5 34.0 39.0 30.0 41.0 68-69 34.916683786616204 36.0 34.0 39.0 29.0 41.0 70-71 34.52779432003635 35.5 34.0 38.5 29.0 40.0 72-73 34.12341371137929 35.0 34.0 37.5 29.0 39.5 74-75 33.71676961587892 35.0 33.5 37.0 28.5 39.0 76-77 33.004172021627625 35.0 32.5 36.0 27.0 39.0 78-79 33.06936600728397 35.0 33.0 36.0 28.0 37.5 80-81 32.91595842536185 35.0 33.0 36.0 28.0 37.0 82-83 32.666069787003465 35.0 33.0 35.0 27.0 37.0 84-85 32.412326279352044 35.0 33.0 35.0 27.0 36.0 86-87 32.05914757546385 35.0 33.0 35.0 26.0 36.0 88-89 31.59304479450018 35.0 32.0 35.0 25.0 36.0 90-91 31.39545537707403 34.5 32.0 35.0 25.0 35.0 92-93 31.130775664396904 34.0 32.0 35.0 24.0 35.0 94-95 30.99472321467967 34.0 32.0 35.0 24.0 35.0 96-97 30.755696165534125 34.0 32.0 35.0 23.0 35.0 98-99 30.33555672530167 34.0 31.0 35.0 18.5 35.0 100-101 27.00913432448239 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1581619349012442 1101 2 0.12557296511131 1101 3 0.19729655595232032 1101 4 0.18020374506715342 1101 5 0.128907648228747 1101 6 0.1530574085524563 1101 7 0.18992190457282732 1101 8 0.13865600378995424 1101 9 0.19384485755765724 1101 10-11 0.14462534156952955 1101 12-13 0.28168808745454044 1101 14-15 0.41547209138545327 1101 16-17 0.43777184702745586 1101 18-19 0.30067389761298813 1101 20-21 0.2610156627378899 1101 22-23 0.31880495551092736 1101 24-25 0.2047320632968379 1101 26-27 0.15411783081838593 1101 28-29 0.3187729242017028 1101 30-31 0.2506431355150127 1101 32-33 0.33667913174105735 1101 34-35 0.33581125828838765 1101 36-37 0.293836329828963 1101 38-39 0.27377416534519483 1101 40-41 0.3764971845569818 1101 42-43 0.37872127576195425 1101 44-45 0.42619885078166675 1101 46-47 0.41045853548222766 1101 48-49 0.40750373016654606 1101 50-51 0.33741574086494097 1101 52-53 0.3088900846510185 1101 54-55 0.32071011795985527 1101 56-57 0.26164095280984867 1101 58-59 0.20056625450970245 1101 60-61 0.2894617274632125 1101 62-63 0.274512383466309 1101 64-65 0.3637596617104677 1101 66-67 0.2652182327369843 1101 68-69 0.31467383400000415 1101 70-71 0.2660979657921487 1101 72-73 0.22377442305812423 1101 74-75 0.20321860041710238 1101 76-77 0.187745205327424 1101 78-79 0.09149389241540007 1101 80-81 0.2581958868853107 1101 82-83 0.15725507290545693 1101 84-85 0.3346295917691151 1101 86-87 0.36592571992279943 1101 88-89 0.2945489353875068 1101 90-91 0.49799359654376474 1101 92-93 0.7325691441027757 1101 94-95 0.5171703337133202 1101 96-97 0.4156315936006969 1101 98-99 0.4416136152759442 1101 100-101 0.8103488437483222 1102 1 0.1006689133351486 1102 2 0.19194102992728546 1102 3 0.1374645531403189 1102 4 0.12697205280945667 1102 5 0.09462024029622995 1102 6 0.11752658486835799 1102 7 0.16636331686991923 1102 8 0.1817756226054712 1102 9 0.11411270131539908 1102 10-11 0.1645928872100697 1102 12-13 0.24554646046512119 1102 14-15 0.2716272007465079 1102 16-17 0.26071949388991555 1102 18-19 0.3452208362874458 1102 20-21 0.34687849620898703 1102 22-23 0.385239675741893 1102 24-25 0.24976567982808717 1102 26-27 0.3369019110886029 1102 28-29 0.2692881798922713 1102 30-31 0.29525707344227214 1102 32-33 0.2679804360259226 1102 34-35 0.25806083580996386 1102 36-37 0.14928278946859308 1102 38-39 0.23748488457726324 1102 40-41 0.17125720443277004 1102 42-43 0.1808543571047423 1102 44-45 0.2219911699951851 1102 46-47 0.311118291467416 1102 48-49 0.27219466222046407 1102 50-51 0.1638138001264764 1102 52-53 0.17963241475928982 1102 54-55 0.10897754675448112 1102 56-57 0.23299533386347804 1102 58-59 0.11575581032823123 1102 60-61 0.1471496657704492 1102 62-63 0.3047734997917573 1102 64-65 0.23944642861142285 1102 66-67 0.3103840303454035 1102 68-69 0.19639200724277828 1102 70-71 0.2745422293888211 1102 72-73 0.2377186671051632 1102 74-75 0.31368818658766884 1102 76-77 0.1557811094607331 1102 78-79 0.20289911038008057 1102 80-81 0.2080934625143982 1102 82-83 0.3020530328273239 1102 84-85 0.43958274239423645 1102 86-87 0.3211713488109922 1102 88-89 0.42949368261662 1102 90-91 0.5557970054107315 1102 92-93 0.5340164163785026 1102 94-95 0.44007673968674865 1102 96-97 0.23454071805406684 1102 98-99 0.4435701506404506 1102 100-101 0.9509961901358359 1103 1 -0.02915379802342244 1103 2 -0.014491998344340118 1103 3 0.06450594120833131 1103 4 -0.014482285147678908 1103 5 0.10337153675173738 1103 6 0.01171020565119818 1103 7 0.12876958432404706 1103 8 0.08391806298338622 1103 9 0.10921290762603064 1103 10-11 0.12896672641898732 1103 12-13 0.1344665654265782 1103 14-15 0.17675207628054324 1103 16-17 0.20380316695781175 1103 18-19 0.09765123774095485 1103 20-21 0.08624156619259793 1103 22-23 0.1496606779709424 1103 24-25 0.13651040607299336 1103 26-27 0.10225101863131414 1103 28-29 0.21844928337841196 1103 30-31 0.14124362704173876 1103 32-33 0.13103018087848994 1103 34-35 0.2020420162590142 1103 36-37 0.1364917506150647 1103 38-39 0.2164869334877224 1103 40-41 0.23870089872163902 1103 42-43 0.14238618552496263 1103 44-45 0.12074520108095754 1103 46-47 0.16121595066802996 1103 48-49 0.1586591631993599 1103 50-51 0.08684896923664098 1103 52-53 0.1236720094431476 1103 54-55 0.11733481090029585 1103 56-57 0.07937510446723195 1103 58-59 4.730638501300177E-4 1103 60-61 0.026574627985844756 1103 62-63 -0.010255427723855348 1103 64-65 0.05972904032432069 1103 66-67 0.041419426973746454 1103 68-69 -0.09046730298390315 1103 70-71 -0.2065476248451219 1103 72-73 -0.06510365165987508 1103 74-75 0.029404905748819488 1103 76-77 0.02619272646938242 1103 78-79 -0.08865578350984293 1103 80-81 -0.08051103292617512 1103 82-83 0.012814661408519612 1103 84-85 -0.13178242704876197 1103 86-87 -0.12003227793604765 1103 88-89 -0.02053337113531839 1103 90-91 0.04092103878223696 1103 92-93 0.12280787573778085 1103 94-95 0.09240226408878982 1103 96-97 0.10158095126594802 1103 98-99 0.10984348839817315 1103 100-101 0.4159058768369839 1104 1 -0.0025180404292584058 1104 2 0.005890041581984917 1104 3 -0.021483373460647215 1104 4 0.013156493773024636 1104 5 0.030406542725550878 1104 6 7.285438926629695E-5 1104 7 0.052417059254786125 1104 8 0.07783249096720368 1104 9 0.020194462977023875 1104 10-11 0.03298027022608352 1104 12-13 0.10354941078915658 1104 14-15 0.1405110680086139 1104 16-17 0.18051466905865965 1104 18-19 0.25823804562767805 1104 20-21 0.11666738251418707 1104 22-23 0.16069530603508042 1104 24-25 0.13630171037880956 1104 26-27 0.21599346984376666 1104 28-29 0.18752850571313218 1104 30-31 0.09380030954475416 1104 32-33 0.11100510209389114 1104 34-35 0.10091685244474036 1104 36-37 0.07193218120914224 1104 38-39 0.2499131742609606 1104 40-41 0.26898197972538895 1104 42-43 0.2327554157253715 1104 44-45 0.19079779165270594 1104 46-47 0.2739334101414457 1104 48-49 0.18602417127174675 1104 50-51 0.33181182251784946 1104 52-53 0.23288200636172007 1104 54-55 0.14752118224002686 1104 56-57 0.26618018566160373 1104 58-59 0.3460339768420013 1104 60-61 0.32301392550117924 1104 62-63 0.22437868408668038 1104 64-65 0.10387930419687308 1104 66-67 0.1546277138231673 1104 68-69 0.3176406338903206 1104 70-71 0.24504040864688648 1104 72-73 0.16034766381044818 1104 74-75 0.23814152156396062 1104 76-77 0.20478621503367123 1104 78-79 0.28732513966924245 1104 80-81 0.23478408080763558 1104 82-83 0.20366118372447062 1104 84-85 0.31620891521524186 1104 86-87 0.21331085615211975 1104 88-89 0.327705563278311 1104 90-91 0.5208691588813359 1104 92-93 0.4799273159112367 1104 94-95 0.3714149369757287 1104 96-97 0.1691716015130531 1104 98-99 0.20325336794921967 1104 100-101 0.3270918081181797 1105 1 -0.026442221416928646 1105 2 0.06852197540325022 1105 3 0.07167004463322257 1105 4 0.04667967938764406 1105 5 -0.05593225598078533 1105 6 -0.012600864710982762 1105 7 0.046056753873060075 1105 8 0.024737613985713836 1105 9 0.0794781325063525 1105 10-11 0.09280995099816636 1105 12-13 0.10129189918913539 1105 14-15 0.15077626077144402 1105 16-17 0.13789318563863162 1105 18-19 0.10040220381711151 1105 20-21 0.11101974878360465 1105 22-23 -0.004260132372948533 1105 24-25 0.10628029803093852 1105 26-27 0.173657508581897 1105 28-29 0.11871362946448727 1105 30-31 0.09571270938752008 1105 32-33 0.11425513878185001 1105 34-35 0.10612314220401942 1105 36-37 0.06254994848753626 1105 38-39 0.03812851928110916 1105 40-41 0.13238692760307913 1105 42-43 0.1686483445532545 1105 44-45 0.1551820769049428 1105 46-47 0.15791822014494983 1105 48-49 0.12993035928427332 1105 50-51 0.06772260101560335 1105 52-53 0.17023247682931952 1105 54-55 0.14655917754795666 1105 56-57 0.10689129183867152 1105 58-59 0.11306857527068814 1105 60-61 0.15049192315279214 1105 62-63 0.20895055594564127 1105 64-65 0.10602743214926136 1105 66-67 0.08864579241867432 1105 68-69 0.16541510294880624 1105 70-71 0.033069366108257725 1105 72-73 0.02989804985490707 1105 74-75 0.057678547565885196 1105 76-77 0.06343705571865854 1105 78-79 -0.10865051258290492 1105 80-81 -0.09113823829983403 1105 82-83 -0.08634706449188201 1105 84-85 0.015985812481858375 1105 86-87 0.016202384760475752 1105 88-89 0.10865682733061632 1105 90-91 0.22881064751840796 1105 92-93 0.24044551504643508 1105 94-95 0.02864082436644466 1105 96-97 -0.09990809018831825 1105 98-99 -0.021116846176074233 1105 100-101 0.10702551400895288 1106 1 0.047132172708693076 1106 2 0.009124674244411324 1106 3 -0.04909972532767881 1106 4 -0.06673128093763836 1106 5 -0.012461039651569195 1106 6 0.01773578485873628 1106 7 0.05972526274828027 1106 8 0.012375621695625227 1106 9 -0.07807963529707251 1106 10-11 0.05286261027190875 1106 12-13 0.01744243367399889 1106 14-15 0.14396029080388217 1106 16-17 -0.012423620609872899 1106 18-19 0.06909309626474425 1106 20-21 0.15838306591923867 1106 22-23 0.0168091825849217 1106 24-25 0.09176834568195602 1106 26-27 0.05468144068431968 1106 28-29 0.025357351716365883 1106 30-31 0.07081290917316352 1106 32-33 0.07816800113690192 1106 34-35 0.15756100445673837 1106 36-37 0.1700666362918284 1106 38-39 0.20017852779606926 1106 40-41 0.09449367580928225 1106 42-43 0.14900708674543495 1106 44-45 0.09870940119919425 1106 46-47 0.1166458114369533 1106 48-49 0.11085396258654612 1106 50-51 0.09511184739825751 1106 52-53 0.01831673593814287 1106 54-55 0.1612382259298215 1106 56-57 0.18517373680774796 1106 58-59 0.20159539507372415 1106 60-61 0.18627311846064742 1106 62-63 0.1359876461043612 1106 64-65 0.18086100788158177 1106 66-67 0.1713713191226276 1106 68-69 0.2210583043379586 1106 70-71 0.08783562473910678 1106 72-73 -0.06374085864319312 1106 74-75 0.1471477071369094 1106 76-77 0.11324475292000358 1106 78-79 0.008810391127248351 1106 80-81 0.13815769126571809 1106 82-83 0.11522262389626547 1106 84-85 0.17684991073362255 1106 86-87 0.028693969900352556 1106 88-89 0.15046580293228473 1106 90-91 0.3185691554914136 1106 92-93 0.3309988413696807 1106 94-95 0.24028489985114376 1106 96-97 0.26356226129880866 1106 98-99 0.2861235993208133 1106 100-101 0.5941188135648048 1107 1 0.016569532525331 1107 2 0.07422250529968721 1107 3 0.08682228200968467 1107 4 0.05267877371755958 1107 5 0.03615020692452475 1107 6 0.0777195876732506 1107 7 0.03751764479597597 1107 8 0.08729323685375334 1107 9 0.015055188642563166 1107 10-11 0.04507058814131426 1107 12-13 0.0878200105270679 1107 14-15 0.15880618446568917 1107 16-17 0.1540509071042493 1107 18-19 0.12984160844711568 1107 20-21 0.08432174208918752 1107 22-23 0.054860983356427084 1107 24-25 0.07595360646296001 1107 26-27 0.15523884512701613 1107 28-29 0.14429026435880843 1107 30-31 0.073862725821499 1107 32-33 0.1239259659512939 1107 34-35 0.13611163934410087 1107 36-37 0.14795591205344039 1107 38-39 0.22930818504783446 1107 40-41 0.189600076188583 1107 42-43 0.055753206257513455 1107 44-45 0.09307624061690944 1107 46-47 0.13341556733714555 1107 48-49 0.13509662327694372 1107 50-51 0.17745112723984846 1107 52-53 0.1502640404108817 1107 54-55 0.19441489000379875 1107 56-57 0.006587036944452507 1107 58-59 -0.007027028164799276 1107 60-61 0.18255937839168013 1107 62-63 0.15603319713974173 1107 64-65 0.1318055887984073 1107 66-67 -0.03972619817285761 1107 68-69 0.12357631370148425 1107 70-71 0.2768324918118239 1107 72-73 0.2103802498904912 1107 74-75 0.10954035361835679 1107 76-77 0.09864031509632554 1107 78-79 0.06322491177673584 1107 80-81 0.29095536904026886 1107 82-83 0.37255552276477744 1107 84-85 0.258138195999706 1107 86-87 0.2929935542739486 1107 88-89 0.34159112634252864 1107 90-91 0.16360277110782562 1107 92-93 0.325867275183537 1107 94-95 0.35937405131772593 1107 96-97 0.10438397677362232 1107 98-99 0.19437152560758975 1107 100-101 0.3612962694564139 1108 1 -0.13758781280810695 1108 2 -0.06332860737221324 1108 3 -0.03258485223910412 1108 4 -0.020082118750629263 1108 5 -0.05962492346883863 1108 6 -0.05969077264749956 1108 7 -0.02087896128224287 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-1.1449587374445862 2316 84-85 -0.9999669601201617 2316 86-87 -1.086507111107636 2316 88-89 -0.9980023684890362 2316 90-91 -1.0163742998969063 2316 92-93 -1.028700917052273 2316 94-95 -0.9930575721330719 2316 96-97 -0.9519157763514983 2316 98-99 -1.1164464231024986 2316 100-101 -1.1486470224334049 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 17.0 4 33.0 5 57.0 6 126.0 7 258.0 8 557.0 9 955.0 10 1486.0 11 2294.0 12 3279.0 13 4493.0 14 5434.0 15 6918.0 16 8350.0 17 10457.0 18 9944.0 19 8489.0 20 9137.0 21 9973.0 22 11184.0 23 13291.0 24 16170.0 25 20150.0 26 25290.0 27 32255.0 28 40920.0 29 51533.0 30 64149.0 31 80498.0 32 101777.0 33 133590.0 34 185706.0 35 278884.0 36 488049.0 37 900965.0 38 893483.0 39 138022.0 40 50.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.752883839088334 17.33865365892148 11.903805808116033 25.004656693874157 2 17.104774562278617 19.38963703566902 36.83715642943822 26.668431972614144 3 20.978678426081306 22.034288425106098 27.764867165585557 29.22216598322704 4 13.534802585669773 14.087240639790547 34.77650526512221 37.60145150941747 5 14.906103517285436 36.661910552643214 33.19613953803397 15.235846392037383 6 36.30931921993735 33.3950026234976 15.681883140114444 14.61379501645061 7 31.948804840163376 30.244248048255496 18.988782896650495 18.81816421493064 8 28.54236112535822 33.40006132267559 18.548900899239985 19.508676652726205 9 27.12367704477539 15.295089380188504 18.66193472198373 38.919298853052375 10-11 27.03631424493946 24.75834813943384 26.12147428853432 22.08386332709238 12-13 27.22604794584263 21.61375214538268 26.820775426385584 24.339424482389102 14-15 23.699555986731593 23.557757838384116 24.360390722682954 28.382295452201333 16-17 24.52159083851128 26.717519171918152 24.76380607155474 23.997083918015825 18-19 24.390405671139025 26.08591301229517 25.86696970148335 23.656711615082454 20-21 24.933485094393976 24.98148655579678 25.631459505792915 24.45356884401633 22-23 24.50585987148373 24.971133793759336 25.81075150780874 24.7122548269482 24-25 24.64922684323832 25.095386915635697 25.746626046192674 24.508760194933306 26-27 24.587112517275518 25.11710815404386 26.147280498181285 24.14849883049934 28-29 24.184257368323205 25.844149219531985 25.846228949931476 24.125364462213337 30-31 24.33719756718801 25.300679812500015 25.67155689119206 24.690565729119914 32-33 24.313506441860252 25.34073319941627 25.85168369436839 24.494076664355084 34-35 24.788626824748285 25.121481259144467 25.805106681483302 24.284785234623943 36-37 24.64754542609664 25.08983061636499 25.9370649603475 24.325558997190875 38-39 24.394683636940023 25.426814290957672 25.96432993781726 24.214172134285043 40-41 24.52163008664344 25.414538544521132 25.783306098147563 24.280525270687864 42-43 24.367005537805696 25.468594368660924 25.40362970224458 24.760770391288805 44-45 24.467728261451523 25.109156652003772 25.652287494261294 24.77082759228341 46-47 24.636564378137198 25.48827702282983 25.551871229671637 24.32328736936134 48-49 24.807913522963876 25.423337921962386 25.408806092125335 24.3599424629484 50-51 24.065447221650743 25.53437144113061 25.94947684268631 24.450704494532342 52-53 24.645675864137175 25.695529839900328 25.28160010298004 24.37719419298246 54-55 24.35700131330042 25.184130452072683 25.516432274533468 24.94243596009343 56-57 24.44411655593403 25.364672917158497 25.603324227400986 24.587886299506483 58-59 25.009945392033934 25.179006517516466 25.365126923718456 24.445921166731143 60-61 24.662861350465686 25.33161616331945 25.91522913399547 24.090293352219394 62-63 24.42491724108664 25.380603879403985 25.792620875509066 24.401858004000307 64-65 24.26716695784899 25.703710126020376 25.497706772353222 24.531416143777413 66-67 24.397150603376343 25.48586697813265 25.29728991433093 24.819692504160077 68-69 24.121662277308335 25.415705630922364 25.651244285426422 24.81138780634288 70-71 24.472278273968445 25.247567604026735 25.585784465266343 24.694369656738484 72-73 24.443653463340272 25.215342497784675 25.743043683422567 24.597960355452482 74-75 24.105600907418218 25.80367975398984 25.521362184031542 24.569357154560404 76-77 24.389815333543307 25.317505249222066 25.3557735670113 24.936905850223322 78-79 24.2910446448981 25.493893196884176 25.587550772801603 24.627511385416117 80-81 24.532493391996198 25.41184620810891 25.5011473546204 24.554513045274486 82-83 24.357271191523193 25.389990576767584 25.739926092405007 24.51281213930421 84-85 24.329535349856275 25.5177190515215 25.36934363515976 24.783401963462463 86-87 24.244340263869407 25.491243020959036 25.569258860777396 24.69515785439416 88-89 24.19116353128001 25.5390299630312 25.226092014216484 25.0437144914723 90-91 24.330634997624173 25.349019568043573 25.44043004223206 24.87991539210019 92-93 24.462800925694303 25.513712843945026 25.493277780024854 24.53020845033582 94-95 24.3051718507335 25.35259935932927 25.493796488395926 24.848432301541308 96-97 23.93552290921191 25.375281186869962 25.87565610269658 24.81353980122154 98-99 24.127090345181415 25.585601587582413 25.21579898567064 25.07150908156553 100-101 23.919859344375265 25.404587270958245 25.385167306785412 25.290386077881085 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 87.0 1 73.0 2 104.5 3 168.5 4 136.0 5 107.5 6 241.0 7 444.5 8 610.0 9 643.0 10 514.5 11 443.0 12 477.0 13 526.0 14 583.5 15 654.5 16 740.5 17 799.5 18 852.0 19 937.5 20 1068.0 21 1284.0 22 1566.0 23 1926.0 24 2489.5 25 3324.0 26 4716.0 27 6612.5 28 8940.0 29 11891.5 30 15860.5 31 20913.5 32 26538.0 33 31606.5 34 37910.0 35 43740.0 36 48773.5 37 56329.0 38 64445.5 39 70338.0 40 76034.0 41 84792.5 42 93804.0 43 101947.5 44 109595.0 45 117618.0 46 125798.0 47 141003.0 48 168866.0 49 171975.5 50 153862.5 51 148010.0 52 150603.5 53 155752.0 54 158209.0 55 153492.5 56 143045.5 57 132667.0 58 126040.0 59 116150.5 60 98518.5 61 81130.0 62 66850.0 63 53117.5 64 40225.0 65 30667.5 66 23342.0 67 18176.5 68 13101.5 69 8779.5 70 6318.0 71 4707.0 72 3707.0 73 3414.5 74 2785.0 75 1990.5 76 1263.5 77 684.5 78 403.5 79 192.0 80 82.5 81 56.0 82 38.5 83 19.5 84 6.0 85 5.0 86 4.0 87 2.0 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26901038073175204 2 0.021639990928066143 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.529349588994744E-4 12-13 1.1241553728865529E-4 14-15 0.0 16-17 7.869087610205869E-4 18-19 0.0 20-21 5.620776864432764E-5 22-23 1.405194216108191E-5 24-25 0.002445037936028252 26-27 0.0023747782252228427 28-29 0.002065635497679041 30-31 0.0011241553728865527 32-33 0.0015316616955579282 34-35 0.0018267524809406483 36-37 7.025971080540955E-5 38-39 1.405194216108191E-4 40-41 6.604412815708498E-4 42-43 0.003906439920780771 44-45 0.0042858423591299825 46-47 0.01441729265727004 48-49 0.014599967905364105 50-51 0.006042335129265221 52-53 0.007194594386473938 54-55 4.4966214915462114E-4 56-57 0.0030071156224715286 58-59 0.0013068306209806175 60-61 0.0013208825631416995 62-63 0.0 64-65 9.133762404703241E-4 66-67 0.0 68-69 0.0 70-71 1.9672719025514674E-4 72-73 0.0016300252906855015 74-75 0.0 76-77 0.19634778781679754 78-79 0.1064996696388398 80-81 0.0011803631415308804 82-83 0.0 84-85 6.463893394097679E-4 86-87 0.12538547990333387 88-89 0.5841251836940139 90-91 0.31028093485884967 92-93 0.2923787605456313 94-95 0.0022764146300952694 96-97 0.002669869010605563 98-99 0.0031616869862434297 100-101 9.274281826314061E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3558227.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.07644733941951 #Duplication Level Percentage of deduplicated Percentage of total 1 61.14047308353939 21.445905844219652 2 15.72764730332474 11.033399848160668 3 7.2627034960481005 7.642495101628476 4 4.171555114682396 5.852933332145726 5 2.70378562625141 4.741959706814349 6 1.8193502784371467 3.828980654013528 7 1.3477137525839242 3.309110732978503 8 0.9987017543522769 2.802472759545881 9 0.7371165464514194 2.3269886752156186 >10 3.7803356449632384 23.054167060787233 >50 0.20635364248887816 4.981462249103577 >100 0.09874477837249121 6.092828562872874 >500 0.0031194226371619245 0.7172788031172039 >1k 0.00215960028062927 1.4717037003836486 >5k 1.599703911577237E-4 0.31464764754847147 >10k+ 7.998519557886185E-5 0.38366532146458737 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13675 0.3843206181055902 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5728 0.16097904939735436 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5487 0.15420601327571287 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4948 0.13905801962606656 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3801 0.10682286430854467 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 2.8103884322163818E-5 0.0 0.0 0.0 0.0 6 5.6207768644327636E-5 0.0 0.0 0.0 0.0 7 5.6207768644327636E-5 0.0 0.0 0.0 0.0 8 5.6207768644327636E-5 0.0 0.0 0.0 0.0 9 5.6207768644327636E-5 0.0 0.0 0.0 0.0 10-11 5.6207768644327636E-5 0.0 0.0 0.0 0.0 12-13 5.6207768644327636E-5 0.0 0.0 0.0 0.0 14-15 1.6862330593298293E-4 0.0 0.0 1.4051942161081909E-5 0.0 16-17 1.6862330593298293E-4 0.0 0.0 4.2155826483245725E-5 0.0 18-19 2.2483107457731057E-4 0.0 0.0 5.6207768644327636E-5 0.0 20-21 2.810388432216382E-4 0.0 0.0 9.836359512757337E-5 0.0 22-23 3.09142727543802E-4 0.0 0.0 2.3888301673839247E-4 0.0 24-25 3.9345438051029347E-4 0.0 0.0 3.09142727543802E-4 0.0 26-27 4.075063226713754E-4 0.0 0.0 4.7776603347678495E-4 0.0 28-29 6.744932237319316E-4 0.0 0.0 0.0015176097533968463 0.0 30-31 7.869087610205869E-4 0.0 0.0 0.004904127814217587 0.0 32-33 7.869087610205869E-4 0.0 0.0 0.01074973575322766 0.0 34-35 8.571684718259964E-4 0.0 0.0 0.019616511256870348 0.0 36-37 9.27428182631406E-4 0.0 0.0 0.03421647916223445 0.0 38-39 0.0010117398355978975 2.8103884322163818E-5 0.0 0.057753482282046645 0.0 40-41 0.0010398437199200614 2.8103884322163818E-5 0.0 0.08564658747179424 0.0 42-43 0.0011101034307254708 2.8103884322163818E-5 0.0 0.11282304361132665 0.0 44-45 0.0012084670258530443 2.8103884322163818E-5 0.0 0.14598562711147994 0.0 46-47 0.0013630383896249452 2.8103884322163818E-5 0.0 0.18538727293115365 0.0 48-49 0.0014332981004303547 2.8103884322163818E-5 0.0 0.2287515664402524 0.0 50-51 0.0014332981004303547 2.8103884322163818E-5 0.0 0.2752775469355946 0.0 52-53 0.0014332981004303547 2.8103884322163818E-5 0.0 0.322688799787085 0.0 54-55 0.00154571363771901 2.8103884322163818E-5 0.0 0.3760159202883908 0.0 56-57 0.00154571363771901 2.8103884322163818E-5 0.0 0.43951664691431996 0.0 58-59 0.00154571363771901 2.8103884322163818E-5 0.0 0.5087646178841316 0.0 60-61 0.00154571363771901 2.8103884322163818E-5 0.0 0.5815115224520526 0.0 62-63 0.00154571363771901 2.8103884322163818E-5 0.0 0.6565629455343911 0.0 64-65 0.00154571363771901 2.8103884322163818E-5 0.0 0.736504444488786 0.0 66-67 0.0016862330593298291 2.8103884322163818E-5 0.0 0.8324089497381701 0.0 68-69 0.0017705447122963206 2.8103884322163818E-5 0.0 0.9391756062780705 0.0 70-71 0.0017845966544574026 2.8103884322163818E-5 0.0 1.0590948806807434 0.0 72-73 0.00184080442310173 2.8103884322163818E-5 0.0 1.1793654536374436 0.0 74-75 0.0018829602495849759 2.8103884322163818E-5 0.0 1.3052005956899322 0.0 76-77 0.0018829602495849759 2.8103884322163818E-5 0.0 1.4440337842414213 0.0 78-79 0.0018829602495849759 2.8103884322163818E-5 0.0 1.6096078187254494 0.0 80-81 0.0020937393820012044 2.8103884322163818E-5 0.0 1.7944049100858377 0.0 82-83 0.0021358952084844503 2.8103884322163818E-5 0.0 1.9780918980155007 0.0 84-85 0.002149947150645532 2.8103884322163818E-5 0.0 2.1665143904534476 0.0 86-87 0.002178051034967696 2.8103884322163818E-5 0.0 2.3680332929855235 0.0 88-89 0.002192102977128778 2.8103884322163818E-5 0.0 2.601660883355671 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12635 0.0 29.444431 1 TACATGG 13310 0.0 27.413132 2 ACATGGG 13245 0.0 26.25214 3 CATGGGG 7850 0.0 20.908321 4 AGTACAT 9175 0.0 20.73713 2 CATGGGA 6410 0.0 20.203068 4 GAGTACA 10500 0.0 20.07179 1 GTATAGG 935 0.0 18.317348 1 GTGTAGC 1795 0.0 18.287584 1 TAGCCCT 1720 0.0 16.547619 4 TACCTGG 3165 0.0 16.490328 2 TATACAG 1530 0.0 15.502344 5 TAGCGCG 460 1.9281288E-10 15.468427 4 GTACCTG 3525 0.0 15.385705 1 ATGGGAG 3315 0.0 15.311545 5 ACTATAC 745 0.0 15.281559 3 GCACCGT 1410 0.0 15.139523 6 TACCGTA 220 5.762576E-4 15.093646 7 ATGGGGA 4275 0.0 15.091124 5 AGAGTAC 10695 0.0 14.991832 5 >>END_MODULE