FastQCFastQC Report
Thu 26 May 2016
SRR936317_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936317_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2282667
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38650.036.146731
GGTATCA25350.034.000861
TCAACGC46300.030.0608354
ATCAACG46200.029.8184933
TACATGG75400.029.7612512
GTACATG77850.029.6255781
CAACGCA47400.029.0635935
ACATGGG75200.028.2029693
AACGCAG50200.027.6274956
GTACCTG22250.027.4571951
TATCAAC50200.027.443122
GTGTAGC14000.026.3853591
TACCTGG22700.024.8179152
AGTACAT51500.024.2683852
GAGTACA60350.023.8557361
CATGGGG45500.022.9940554
ACGCAGA60450.022.8651287
CCGTACG1050.002240757722.5422029
CGCAGAG61750.022.3085678
TAGCCCT15050.021.0752624
Produced by FastQC (version 0.11.5)