##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936317_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2282667 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.160901261550634 31.0 31.0 34.0 28.0 34.0 2 31.249643509105795 33.0 31.0 34.0 28.0 34.0 3 31.322235350140865 34.0 31.0 34.0 28.0 34.0 4 34.80963189111684 37.0 35.0 37.0 32.0 37.0 5 34.762737622263785 37.0 35.0 37.0 32.0 37.0 6 34.83935939845803 37.0 35.0 37.0 32.0 37.0 7 34.7663014359957 37.0 35.0 37.0 32.0 37.0 8 34.757595829790326 37.0 35.0 37.0 32.0 37.0 9 36.362361220449586 39.0 37.0 39.0 32.0 39.0 10-11 36.25425916263739 39.0 37.0 39.0 32.0 39.0 12-13 36.13714637308026 39.0 37.0 39.0 32.0 39.0 14-15 37.30541336077492 40.0 37.0 41.0 31.5 41.0 16-17 37.227939292064946 40.0 37.0 41.0 31.0 41.0 18-19 37.140524439175756 40.0 37.0 41.0 31.0 41.0 20-21 37.03234505952905 40.0 37.0 41.0 31.0 41.0 22-23 36.85410136476324 40.0 37.0 41.0 30.5 41.0 24-25 37.016288622037294 40.0 37.0 41.0 31.0 41.0 26-27 36.95693896656849 40.0 37.0 41.0 30.5 41.0 28-29 36.80410808935338 40.0 37.0 41.0 30.0 41.0 30-31 36.65429320176793 40.0 36.5 41.0 30.0 41.0 32-33 36.47380914517974 40.0 36.0 41.0 30.0 41.0 34-35 36.20482794906134 40.0 36.0 41.0 29.5 41.0 36-37 36.01145436456566 40.0 36.0 41.0 28.0 41.0 38-39 35.83812005868574 39.5 35.0 41.0 27.5 41.0 40-41 35.643184047432236 39.0 35.0 41.0 27.0 41.0 42-43 35.45438909836608 39.0 35.0 41.0 26.0 41.0 44-45 35.28383268343565 39.0 35.0 41.0 26.0 41.0 46-47 35.02967165162505 39.0 35.0 40.5 24.5 41.0 48-49 34.87393123920397 39.0 34.5 40.5 24.0 41.0 50-51 34.583766927020015 38.5 34.5 40.5 24.0 41.0 52-53 34.614250786470386 38.5 34.0 40.0 24.0 41.0 54-55 34.691609858117715 39.0 34.0 40.5 24.0 41.0 56-57 34.52089244729959 38.0 34.0 40.5 23.0 41.0 58-59 34.26986152601321 38.0 34.0 40.0 23.0 41.0 60-61 33.95142743115838 37.5 34.0 40.0 22.0 41.0 62-63 33.624078544965165 37.0 33.5 40.0 21.5 41.0 64-65 33.27357341215342 36.5 33.0 40.0 20.5 41.0 66-67 32.90337289670372 36.0 33.0 39.0 19.5 41.0 68-69 32.51154723838387 35.5 32.0 39.0 18.0 41.0 70-71 32.09583504733717 35.0 32.0 38.5 15.5 40.0 72-73 31.680866723004275 35.0 32.0 37.5 12.0 40.0 74-75 31.254752445275635 35.0 31.0 37.0 7.5 39.0 76-77 30.843452198678122 35.0 31.0 36.5 7.0 39.0 78-79 30.442204885776157 35.0 31.0 36.0 2.0 38.5 80-81 30.041861340265577 34.5 30.0 35.5 2.0 37.0 82-83 29.685126871330773 34.0 30.0 35.0 2.0 37.0 84-85 29.361764111891922 34.0 30.0 35.0 2.0 36.0 86-87 29.0194886069672 34.0 29.0 35.0 2.0 36.0 88-89 28.674994863464534 34.0 29.0 35.0 2.0 36.0 90-91 28.298823919564263 34.0 29.0 35.0 2.0 35.0 92-93 27.947508331263386 34.0 28.5 35.0 2.0 35.0 94-95 27.532139598110454 34.0 27.0 35.0 2.0 35.0 96-97 27.049634046490354 34.0 26.5 35.0 2.0 35.0 98-99 26.483412385599827 33.5 25.0 35.0 2.0 35.0 100-101 23.249109265609043 29.0 12.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5418573254065073 1101 2 0.3453815623092247 1101 3 0.5582003056321909 1101 4 0.3345450252637079 1101 5 0.6044800326063466 1101 6 0.5585341654732972 1101 7 0.6026060985560804 1101 8 0.42128603989331026 1101 9 0.4189576861816917 1101 10-11 0.7093505188954197 1101 12-13 0.6194389995639469 1101 14-15 0.7464274423882884 1101 16-17 0.6742652403539111 1101 18-19 0.8393232869524923 1101 20-21 1.026108216612137 1101 22-23 0.8916120554956493 1101 24-25 0.8384071832041187 1101 26-27 0.8043540845877786 1101 28-29 0.7532737463648402 1101 30-31 0.8878182597041189 1101 32-33 0.9506929549416299 1101 34-35 0.8268919634022112 1101 36-37 0.6577269514328705 1101 38-39 0.6365229330167992 1101 40-41 0.657689410074596 1101 42-43 0.7128082294621052 1101 44-45 0.5902350770922169 1101 46-47 0.47018458922705264 1101 48-49 0.4785109412634867 1101 50-51 0.5623933448361313 1101 52-53 0.5984180398053738 1101 54-55 0.7335152720432845 1101 56-57 0.6981299530461982 1101 58-59 0.6511800864143922 1101 60-61 0.5551675637505724 1101 62-63 0.5791745047200507 1101 64-65 0.5648118112951224 1101 66-67 0.5479948090493352 1101 68-69 0.6437226388197459 1101 70-71 0.8656870455978449 1101 72-73 0.8716728407496248 1101 74-75 0.7389142553259838 1101 76-77 0.5492860489159774 1101 78-79 0.5831696316125239 1101 80-81 0.6761402123593427 1101 82-83 0.7676898565241288 1101 84-85 0.6877754234432452 1101 86-87 0.6768431659330787 1101 88-89 0.51483521901363 1101 90-91 0.553873117276062 1101 92-93 0.6953907892250974 1101 94-95 0.9226623940061778 1101 96-97 1.096648704422524 1101 98-99 1.1665631259014795 1101 100-101 1.0524656625053659 1102 1 0.3411422774873287 1102 2 0.3426375534534003 1102 3 0.34087111432446093 1102 4 0.23367689525862545 1102 5 0.31090848293693796 1102 6 0.345016568512996 1102 7 0.2793181745885107 1102 8 0.22029624204144227 1102 9 0.20887920044774688 1102 10-11 0.35115638887038614 1102 12-13 0.45697253301761975 1102 14-15 0.4929232588094621 1102 16-17 0.44852002479239417 1102 18-19 0.5258595429735635 1102 20-21 0.46765629037076906 1102 22-23 0.49856158328478983 1102 24-25 0.6320290953838708 1102 26-27 0.49844072805112205 1102 28-29 0.4539025297860988 1102 30-31 0.5206037553011384 1102 32-33 0.27002243545982196 1102 34-35 0.3047755910986041 1102 36-37 0.25551075165825665 1102 38-39 0.28516588775037377 1102 40-41 0.25632862501780096 1102 42-43 0.17978164204574654 1102 44-45 0.16164363173803054 1102 46-47 0.33138625866947535 1102 48-49 0.25466828172402955 1102 50-51 0.21220959646917237 1102 52-53 0.26470856666704634 1102 54-55 0.3227411853000959 1102 56-57 0.290361161846171 1102 58-59 0.19048054615929289 1102 60-61 0.34705695571052075 1102 62-63 0.39653885005728284 1102 64-65 0.19656138495902553 1102 66-67 0.277311275097027 1102 68-69 0.34067944673413564 1102 70-71 0.5747203845824131 1102 72-73 0.6104886236622704 1102 74-75 0.4748551367197784 1102 76-77 0.33378686169708516 1102 78-79 0.4544610061600096 1102 80-81 0.3717028264229505 1102 82-83 0.4062311388234505 1102 84-85 0.5069141746524437 1102 86-87 0.4470138499656713 1102 88-89 0.481332182303845 1102 90-91 0.5300341975269802 1102 92-93 0.5941785985057599 1102 94-95 0.4980670393244573 1102 96-97 0.6103969217891105 1102 98-99 0.7155279519264326 1102 100-101 0.7349148262882395 1103 1 0.3210372420550698 1103 2 0.2569599067363342 1103 3 0.3566359847884044 1103 4 0.25943886081250866 1103 5 0.2477620448917932 1103 6 0.1402309202976042 1103 7 0.3199859599003716 1103 8 0.34097041051094124 1103 9 0.2803839438601017 1103 10-11 0.40303817814209 1103 12-13 0.4510609454842722 1103 14-15 0.5362107187713931 1103 16-17 0.6114477345765934 1103 18-19 0.420576620022473 1103 20-21 0.5100598550012023 1103 22-23 0.501876429668549 1103 24-25 0.6074572829493263 1103 26-27 0.6048330590885556 1103 28-29 0.5493789923700945 1103 30-31 0.53053587213968 1103 32-33 0.5639926448426777 1103 34-35 0.3683486878091813 1103 36-37 0.40230713275187924 1103 38-39 0.5209394729445123 1103 40-41 0.539545411575709 1103 42-43 0.4116068429049875 1103 44-45 0.4679864646369083 1103 46-47 0.48978699101258627 1103 48-49 0.45742001511444386 1103 50-51 0.6025583194554187 1103 52-53 0.5804794571748673 1103 54-55 0.5586619271223867 1103 56-57 0.5452832643894894 1103 58-59 0.5979018269188501 1103 60-61 0.5276845500627658 1103 62-63 0.3624343459177837 1103 64-65 0.4732602795643004 1103 66-67 0.5371425800896503 1103 68-69 0.6487069363567244 1103 70-71 0.5595608629752391 1103 72-73 0.532404832295974 1103 74-75 0.5769073027889 1103 76-77 0.3981979867381753 1103 78-79 0.5150293365247265 1103 80-81 0.48868613465684163 1103 82-83 0.5363051758075414 1103 84-85 0.4614895991133139 1103 86-87 0.46727034205743223 1103 88-89 0.48762551706414925 1103 90-91 0.6702649085419381 1103 92-93 0.6012034163755438 1103 94-95 0.6342165428141726 1103 96-97 0.8055366441408651 1103 98-99 0.7914344940896747 1103 100-101 1.0218024062688222 1104 1 0.4125380567497565 1104 2 0.3143323683368031 1104 3 0.4220054435751699 1104 4 0.38630248684376767 1104 5 0.37564021890599264 1104 6 0.33409267816614374 1104 7 0.4064073490264377 1104 8 0.421059769051098 1104 9 0.3479811580436163 1104 10-11 0.39229946591365206 1104 12-13 0.43160055893886096 1104 14-15 0.4831539661654105 1104 16-17 0.47980326905641846 1104 18-19 0.332058323297872 1104 20-21 0.4749631565681227 1104 22-23 0.5950469864307095 1104 24-25 0.6429515390175098 1104 26-27 0.49963715987765767 1104 28-29 0.5423364916680953 1104 30-31 0.46376576885587895 1104 32-33 0.6181545599195886 1104 34-35 0.6547438357435951 1104 36-37 0.497099199363916 1104 38-39 0.483380185520609 1104 40-41 0.5876932227881255 1104 42-43 0.553250778348314 1104 44-45 0.568340756540195 1104 46-47 0.7371342190449681 1104 48-49 0.7169868277193743 1104 50-51 0.6464647995732378 1104 52-53 0.6643090809253707 1104 54-55 0.8405873572487081 1104 56-57 0.735160372519438 1104 58-59 0.7967190869850569 1104 60-61 0.7253429236532796 1104 62-63 0.6780037773888807 1104 64-65 0.782665776891001 1104 66-67 0.8966954960853641 1104 68-69 0.7626309567651646 1104 70-71 0.8107128250236428 1104 72-73 0.8238406051289076 1104 74-75 0.7387927718066258 1104 76-77 0.774727688233277 1104 78-79 0.6834626872407625 1104 80-81 0.6831661961266526 1104 82-83 0.756879941475372 1104 84-85 0.4698402196375113 1104 86-87 0.5637945899691239 1104 88-89 0.6883508150055455 1104 90-91 0.8876756884903259 1104 92-93 0.6662439648665917 1104 94-95 0.4454387472079837 1104 96-97 0.6198030894168802 1104 98-99 0.646616902606052 1104 100-101 0.9059099383265341 1105 1 0.342257555070109 1105 2 0.3352357643603412 1105 3 0.3043535604410721 1105 4 0.18690520313288062 1105 5 0.22955876926435081 1105 6 0.28046319557699917 1105 7 0.2792282178514256 1105 8 0.36301202431173607 1105 9 0.37026251656455145 1105 10-11 0.517152835230597 1105 12-13 0.47894661617948486 1105 14-15 0.5001150326261481 1105 16-17 0.43988092378329924 1105 18-19 0.4737878416429453 1105 20-21 0.5651833401524158 1105 22-23 0.6600092870402818 1105 24-25 0.5925943398198044 1105 26-27 0.47532626310077575 1105 28-29 0.5191076822848544 1105 30-31 0.6315922380494357 1105 32-33 0.6252877404247812 1105 34-35 0.3200000133996923 1105 36-37 0.5914816149732971 1105 38-39 0.6539374878273563 1105 40-41 0.6002499670328447 1105 42-43 0.6124652955816785 1105 44-45 0.7120635693122352 1105 46-47 0.7351542837216201 1105 48-49 0.7606794605338436 1105 50-51 0.575197931381588 1105 52-53 0.5576290876815833 1105 54-55 0.6550432316647701 1105 56-57 0.7360835446629892 1105 58-59 0.8182178343658038 1105 60-61 0.6924312036303562 1105 62-63 0.48456174974180755 1105 64-65 0.5460113471632511 1105 66-67 0.5914597264929569 1105 68-69 0.6760662885971627 1105 70-71 0.7458885297628299 1105 72-73 0.6734535744719778 1105 74-75 0.6683497537272238 1105 76-77 0.7046471084435346 1105 78-79 0.6476387987936931 1105 80-81 0.601100874059572 1105 82-83 0.7459916911903584 1105 84-85 0.8448163184586335 1105 86-87 0.7943599402645702 1105 88-89 0.7693896932497459 1105 90-91 0.8725378492591247 1105 92-93 0.7211926298971747 1105 94-95 0.6690585252795849 1105 96-97 0.5868985716932968 1105 98-99 0.5240328384135786 1105 100-101 0.6014137984104835 1106 1 0.3272203307840158 1106 2 0.304641519176144 1106 3 0.36984859208105547 1106 4 0.33145036792684834 1106 5 0.37724685968789373 1106 6 0.29295916759227936 1106 7 0.37796279407231026 1106 8 0.26465456404501 1106 9 0.4858191423469904 1106 10-11 0.3641497805474714 1106 12-13 0.32412218802160453 1106 14-15 0.45187228760833165 1106 16-17 0.5532863642626396 1106 18-19 0.4696117982961212 1106 20-21 0.40554607389626085 1106 22-23 0.43496786574775825 1106 24-25 0.3750170925116336 1106 26-27 0.3829285739128139 1106 28-29 0.369161176556112 1106 30-31 0.40651404904892985 1106 32-33 0.4225811102960648 1106 34-35 0.29584132441103606 1106 36-37 0.31714347761879225 1106 38-39 0.28400769988154906 1106 40-41 0.14884359671464154 1106 42-43 0.26317481356689854 1106 44-45 0.37386310606107287 1106 46-47 0.431818278235653 1106 48-49 0.4590492571897613 1106 50-51 0.4581937554480007 1106 52-53 0.42651269714223616 1106 54-55 0.37089437227589883 1106 56-57 0.3120304832628946 1106 58-59 0.30246984089486517 1106 60-61 0.2569465679226113 1106 62-63 0.37858283750401966 1106 64-65 0.3419034832200154 1106 66-67 0.24752415932197636 1106 68-69 0.3245974021999487 1106 70-71 0.4810247173532147 1106 72-73 0.4749405483864422 1106 74-75 0.4094170665104464 1106 76-77 0.4367904046037978 1106 78-79 0.3889094863915368 1106 80-81 0.4345998998799452 1106 82-83 0.4888347196378078 1106 84-85 0.443436640398879 1106 86-87 0.3950120347727264 1106 88-89 0.25991964872460116 1106 90-91 0.4794247592526517 1106 92-93 0.4261165174490138 1106 94-95 0.6852378533546606 1106 96-97 0.6353243869705771 1106 98-99 0.7082844937287227 1106 100-101 1.0981083265853009 1107 1 0.14114383982255063 1107 2 0.31569117670407465 1107 3 0.3674640127297053 1107 4 0.2840944293468226 1107 5 0.18635326024558196 1107 6 0.4305270810761215 1107 7 0.3523124291007278 1107 8 0.4713264068287515 1107 9 0.4353123500765008 1107 10-11 0.46924647311931267 1107 12-13 0.5227702809113879 1107 14-15 0.5655381588004715 1107 16-17 0.3474388888184592 1107 18-19 0.535826753463077 1107 20-21 0.6375280226490077 1107 22-23 0.7105504555521591 1107 24-25 0.7503310713473397 1107 26-27 0.7941707682421963 1107 28-29 0.7571532611542722 1107 30-31 0.6742794418498264 1107 32-33 0.7250506160813899 1107 34-35 0.6366605094861129 1107 36-37 0.6611886684404666 1107 38-39 0.7191616186693821 1107 40-41 0.812366171839038 1107 42-43 0.5179232247694401 1107 44-45 0.6210824799845085 1107 46-47 0.8116515236610624 1107 48-49 0.6600713251530408 1107 50-51 0.7734082200518415 1107 52-53 0.7449918961596396 1107 54-55 0.737023663319988 1107 56-57 0.6937688102582484 1107 58-59 0.8799488444247103 1107 60-61 0.9190527803696398 1107 62-63 0.9105759438012981 1107 64-65 0.6630268358685001 1107 66-67 0.8416970625335054 1107 68-69 0.8929332129384804 1107 70-71 0.9779517820522159 1107 72-73 0.8242800401925621 1107 74-75 0.9060762579296515 1107 76-77 0.9564433588633996 1107 78-79 0.9843147911123751 1107 80-81 1.067368224994194 1107 82-83 1.2576135125574694 1107 84-85 1.177625662112753 1107 86-87 0.985196346343006 1107 88-89 1.1002551865182433 1107 90-91 1.2087731586521713 1107 92-93 1.0297493586209079 1107 94-95 1.1448821213275302 1107 96-97 1.0987921886945564 1107 98-99 1.2144958342200987 1107 100-101 1.2164709707573742 1108 1 0.011709255989806877 1108 2 0.1087119330325308 1108 3 0.16159005072555743 1108 4 0.16359540185114696 1108 5 0.23172902012672836 1108 6 0.12193279839674886 1108 7 0.07828780428643967 1108 8 0.18056465962246193 1108 9 0.20351989182958619 1108 10-11 0.17472165452187483 1108 12-13 0.19506458368680057 1108 14-15 0.16800952195727348 1108 16-17 0.22280447508425283 1108 18-19 0.20674618273581302 1108 20-21 0.21997378131004552 1108 22-23 0.17538374821496205 1108 24-25 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2313 68-69 -0.3098228482917378 2313 70-71 -0.10920396216118178 2313 72-73 -0.23953563930403377 2313 74-75 -0.3177407499330833 2313 76-77 -0.4325873219167846 2313 78-79 -0.5443398575720906 2313 80-81 -0.47058125530120876 2313 82-83 -0.35031101236506856 2313 84-85 -0.3682126712504328 2313 86-87 -0.39340404828621445 2313 88-89 -0.24457336469698632 2313 90-91 -0.26449645788284926 2313 92-93 -0.44951096532132695 2313 94-95 -0.3658615995305077 2313 96-97 -0.34241529612625143 2313 98-99 -0.09678251669543059 2313 100-101 -0.20310951927539733 2314 1 -0.6425027980652338 2314 2 -0.7651329889311818 2314 3 -0.5349210262770328 2314 4 -0.56972840371985 2314 5 -0.6468866351614366 2314 6 -0.5530073594247611 2314 7 -0.66541310489702 2314 8 -0.7484773304007959 2314 9 -0.8373963004067164 2314 10-11 -0.6813211982206866 2314 12-13 -0.8734248548539156 2314 14-15 -1.0208279539949174 2314 16-17 -1.0872727976786152 2314 18-19 -0.9744483637296426 2314 20-21 -1.1019810544861528 2314 22-23 -0.9796526856871637 2314 24-25 -0.8736531505806724 2314 26-27 -0.8746228690662718 2314 28-29 -0.9903542821613911 2314 30-31 -1.0911656676809898 2314 32-33 -1.1786578274008832 2314 34-35 -0.9136700092093761 2314 36-37 -0.854960100550791 2314 38-39 -1.0052741240686984 2314 40-41 -0.892452817335105 2314 42-43 -1.1870511948013487 2314 44-45 -1.3124375135754605 2314 46-47 -1.1755643711364598 2314 48-49 -1.0664448489520097 2314 50-51 -0.9026387159748097 2314 52-53 -0.9233027466846764 2314 54-55 -1.134621016683866 2314 56-57 -1.2398722932292898 2314 58-59 -1.253202536515964 2314 60-61 -1.104129887901287 2314 62-63 -1.2398813989103203 2314 64-65 -1.0045984679844508 2314 66-67 -1.1843552282050709 2314 68-69 -1.0184619374893913 2314 70-71 -0.9078830642567723 2314 72-73 -1.1892113182367616 2314 74-75 -1.185339096652907 2314 76-77 -1.0082527378750399 2314 78-79 -0.9456511471697162 2314 80-81 -1.0389459866268957 2314 82-83 -0.9837630652856646 2314 84-85 -1.1569838176679994 2314 86-87 -1.3381892619163338 2314 88-89 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-1.101860799174112 2315 46-47 -0.8737453412377434 2315 48-49 -1.0895801260260853 2315 50-51 -1.1918051229343405 2315 52-53 -1.3619528507218064 2315 54-55 -1.0897203533764213 2315 56-57 -1.1164845345378396 2315 58-59 -1.1573016921849373 2315 60-61 -1.165863044736625 2315 62-63 -1.2087284998073144 2315 64-65 -1.1187851372430444 2315 66-67 -1.4164758063697178 2315 68-69 -1.1087105517847 2315 70-71 -1.1553356201349487 2315 72-73 -1.3258423407423123 2315 74-75 -1.4014297020430853 2315 76-77 -1.262245927891179 2315 78-79 -1.257948202759387 2315 80-81 -1.1654998581145506 2315 82-83 -1.4423570057268478 2315 84-85 -0.945698051367394 2315 86-87 -0.9636486349781705 2315 88-89 -1.106030216285415 2315 90-91 -1.111076590647194 2315 92-93 -1.1837056863581559 2315 94-95 -1.20327467049621 2315 96-97 -1.2103918252869903 2315 98-99 -1.4048570385857424 2315 100-101 -1.4368399602426827 2316 1 -1.3261472834309025 2316 2 -1.3065916407971017 2316 3 -1.2946843200424567 2316 4 -1.3008813100327785 2316 5 -1.3899269362982736 2316 6 -1.3475664798352867 2316 7 -1.3821979366737054 2316 8 -1.4688124508194562 2316 9 -1.4119721788706912 2316 10-11 -1.5723001012643252 2316 12-13 -1.5943261566264155 2316 14-15 -1.8386086399572221 2316 16-17 -2.0193596174035235 2316 18-19 -1.8953275365383035 2316 20-21 -1.9349754666052021 2316 22-23 -1.9081423419844725 2316 24-25 -1.9223615152273723 2316 26-27 -1.9581381121368082 2316 28-29 -2.297367995005189 2316 30-31 -2.150855308327955 2316 32-33 -2.0936242985907967 2316 34-35 -2.0404090129436057 2316 36-37 -1.9817727828098626 2316 38-39 -1.9484444805050742 2316 40-41 -1.8801276704480685 2316 42-43 -2.171458405337475 2316 44-45 -2.341675057712706 2316 46-47 -2.369435181686619 2316 48-49 -2.2116945657978064 2316 50-51 -2.241740696707616 2316 52-53 -2.4447571709276517 2316 54-55 -2.3767850515028144 2316 56-57 -2.3679826067491874 2316 58-59 -2.5929329442529223 2316 60-61 -2.583899289362357 2316 62-63 -2.6438033321941674 2316 64-65 -2.5138519825771155 2316 66-67 -2.4876630382811413 2316 68-69 -2.6028086046032115 2316 70-71 -2.411419125321533 2316 72-73 -2.4262756558490395 2316 74-75 -2.3543157121053717 2316 76-77 -2.6782877834464784 2316 78-79 -2.694378160105124 2316 80-81 -2.585705427259537 2316 82-83 -2.6331206139554126 2316 84-85 -2.9700406939322868 2316 86-87 -3.0376250876671307 2316 88-89 -2.9615584013851155 2316 90-91 -3.1334475857500017 2316 92-93 -3.126414326869803 2316 94-95 -3.035700755270927 2316 96-97 -3.1568573295159297 2316 98-99 -2.979188860143587 2316 100-101 -2.876362401461872 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37651.0 3 7618.0 4 6013.0 5 4738.0 6 4499.0 7 4731.0 8 5664.0 9 6447.0 10 7444.0 11 8727.0 12 10111.0 13 12949.0 14 12163.0 15 11287.0 16 12128.0 17 13897.0 18 13351.0 19 12077.0 20 12400.0 21 13008.0 22 14061.0 23 15519.0 24 17805.0 25 20470.0 26 23926.0 27 28460.0 28 34105.0 29 40904.0 30 49005.0 31 59743.0 32 74399.0 33 97494.0 34 132886.0 35 194082.0 36 310131.0 37 472310.0 38 413395.0 39 67017.0 40 52.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.24479649233839 17.209420967604796 11.676894716681115 24.868887823375704 2 16.685864353772555 18.720570909567282 38.488814152514486 26.104750584145677 3 20.493454157193984 22.247541119354235 27.47768726551968 29.7813174579321 4 13.012121140430773 14.150194892446619 34.918452724397945 37.91923124272466 5 14.652827340074243 36.652353294507165 33.483871278665625 15.210948086752968 6 36.14438406852683 33.47224224124675 16.227019681049843 14.156354009176574 7 31.997762146096637 30.32318325517493 19.034901408253248 18.644153190475187 8 28.466485868379433 34.35138249352675 18.41614566675575 18.76598597133807 9 27.267676674737267 15.182894182244855 18.4800982142153 39.06933092880258 10-11 26.845196698096156 25.30015366097548 26.217913824997346 21.63673581593102 12-13 26.713617172941035 22.00508702287802 27.123019337127314 24.15827646705363 14-15 23.323422414769425 23.971681045275147 24.37687280982975 28.32802373012568 16-17 24.21109108228401 27.08597507915379 25.063423807741952 23.639510030820247 18-19 24.048859586631373 26.095592481307108 26.427148751893824 23.4283991801677 20-21 24.44402971016207 25.50889648845651 26.533195244341286 23.51387855704013 22-23 24.073835134712997 25.31591414276723 26.474136388161302 24.136114334358467 24-25 23.96019647622261 25.480542982459987 26.672633105630066 23.886627435687338 26-27 23.9965187355641 25.739122472512644 26.4379602401573 23.826398551765955 28-29 23.774149718285237 25.933466329400556 26.567987265327154 23.724396686987053 30-31 23.94002167933339 25.640938012353892 26.524695074129472 23.894345234183245 32-33 23.772644236003693 25.633473412704582 26.677485704171033 23.916396647120692 34-35 24.154973096260093 25.826909537464736 26.303758617827228 23.714358748447946 36-37 24.235510326136346 25.68603449387442 26.265443216206897 23.813011963782333 38-39 23.96878442134298 25.727064576474735 26.46140122892583 23.84274977325645 40-41 24.048541065489637 25.672357271256356 26.570667608009398 23.708434055244613 42-43 24.10984364196664 25.547332336629907 26.35165615756306 23.991167863840396 44-45 23.953219046210485 25.77721758130699 26.48096989993054 23.788593472551987 46-47 24.10577193970982 26.000977250316947 26.151284511903082 23.74196629807015 48-49 24.372061551384206 25.881702425335146 26.089714706204113 23.656521317076532 50-51 24.103210410858182 25.969072539413308 26.10932369707423 23.818393352654283 52-53 24.206370344217646 26.054728100593554 25.978855032033092 23.760046523155705 54-55 24.037361950498056 25.852044387124035 26.14398964711268 23.96660401526523 56-57 23.958725804690996 26.167224489508474 26.09552327362487 23.778526432175667 58-59 24.07141814951984 25.93651539095849 26.086229332356808 23.90583712716486 60-61 24.053715213508113 25.93124334716148 26.299412008460177 23.715629430870226 62-63 24.015368124411477 26.03675313492265 26.181178646854207 23.766700093811664 64-65 23.86781396055196 25.979427504899917 26.15460294996418 23.998155584583944 66-67 23.880972063825823 26.180825452875574 26.033422367818552 23.904780115480055 68-69 23.725915022264793 25.956025932453773 26.34623206447194 23.971826980809492 70-71 23.790244673177142 25.82496271124974 26.285235063264345 24.099557552308777 72-73 23.90507531310679 26.091194408676078 26.030541625393354 23.973188652823776 74-75 23.778173478191967 26.221716469158846 25.92985684351394 24.070253209135252 76-77 23.989526138767218 26.166474796996315 25.92203628482512 23.921962779411356 78-79 23.934411365398468 26.091568536874842 26.04200755430856 23.93201254341813 80-81 23.85342846308697 26.21676563972808 26.02102763990458 23.908778257280368 82-83 24.05611513356329 26.320320806302917 25.878267996331704 23.74529606380209 84-85 23.960932336724543 26.17521995312743 25.727819985391108 24.136027724756918 86-87 23.91033549909788 25.981911809175617 26.20098253904605 23.90677015268046 88-89 23.94979069551425 26.143910976148227 25.925868044626384 23.98043028371114 90-91 23.87813279800688 26.374045414975605 25.95090825107562 23.79691353594189 92-93 23.872299376431187 26.148305735838058 26.057497949395074 23.921896938335678 94-95 23.86521417719981 26.041879638569306 26.169987396098136 23.922918788132748 96-97 23.934875362155577 26.0157517874556 26.023034502909564 24.026338347479257 98-99 23.712357665947035 25.99012052015002 25.91721134047949 24.38031047342346 100-101 23.86705871791848 26.163084402497343 25.678198207078395 24.29165867250578 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 41.0 1 35.0 2 68.5 3 121.0 4 109.5 5 100.5 6 192.0 7 383.5 8 575.0 9 691.0 10 727.0 11 781.0 12 970.0 13 1142.0 14 1318.0 15 1547.0 16 1736.5 17 1892.5 18 1968.5 19 2034.0 20 2063.5 21 2121.5 22 2301.0 23 2579.0 24 3182.5 25 4169.0 26 5658.5 27 7541.5 28 9494.5 29 11933.0 30 14931.5 31 18381.0 32 22750.5 33 26604.0 34 29869.0 35 34014.5 36 39657.0 37 44872.0 38 48725.5 39 52596.5 40 56383.5 41 60809.0 42 65148.5 43 69939.5 44 75346.0 45 80076.0 46 83659.5 47 86183.5 48 89384.0 49 90918.5 50 89771.0 51 89200.0 52 90296.5 53 91731.0 54 93517.0 55 90548.0 56 82930.0 57 76829.5 58 72070.0 59 65692.5 60 57580.0 61 48943.5 62 40726.0 63 33206.5 64 25427.5 65 19066.5 66 14059.5 67 11058.5 68 8418.5 69 5874.5 70 4499.0 71 3341.0 72 2590.0 73 2188.5 74 1725.0 75 1278.0 76 886.5 77 463.5 78 256.5 79 168.0 80 104.0 81 69.5 82 45.5 83 32.5 84 31.5 85 28.0 86 24.0 87 22.5 88 21.0 89 20.5 90 21.0 91 22.5 92 22.0 93 19.0 94 16.0 95 24.0 96 26.0 97 21.5 98 17.0 99 13.5 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04525408217668192 2 0.012003502920049224 3 0.009900699488799724 4 0.005519859007029935 5 0.00814836329609181 6 0.005081774958852956 7 0.005388433792576841 8 0.008630255749086485 9 0.013536797088668649 10-11 0.0019494740143875563 12-13 0.008433117927406846 14-15 0.0073379078069643975 16-17 0.017567170331896857 18-19 0.010908292799606777 20-21 0.0035484807902335298 22-23 0.008915010380401522 24-25 0.020020441001687938 26-27 0.0071407699852847565 28-29 0.01726051149817297 30-31 0.013536797088668649 32-33 0.01185017350318728 34-35 0.2561039345642619 36-37 0.4534388940655821 38-39 0.45017516790666356 40-41 0.4702832257179869 42-43 0.46042633463400484 44-45 0.4751021502479337 46-47 0.48132294373204676 48-49 0.48918655239682357 50-51 0.49873678464708165 52-53 0.4867113775246236 54-55 0.4747516830093921 56-57 0.4704584593372577 58-59 0.4673261583927923 60-61 0.46482907931818346 62-63 0.46268246748211633 64-65 0.4794610865272946 66-67 0.45238749234995734 68-69 0.47834397220444336 70-71 0.46147773634962963 72-73 0.46956038703849484 74-75 0.48307527992475463 76-77 0.46986704587221884 78-79 0.46956038703849484 80-81 0.4710498728022966 82-83 0.47166319046974436 84-85 0.4714879568504736 86-87 0.47304315522150187 88-89 0.4860980598571758 90-91 0.5105431497454513 92-93 0.49880249725430825 94-95 0.4710060643974789 96-97 0.44522481816226367 98-99 0.43705455066376303 100-101 0.4457505190200761 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2282667.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.693720427474915 #Duplication Level Percentage of deduplicated Percentage of total 1 76.3305820624278 42.51134097451285 2 13.011044845466795 14.492669881855324 3 4.458419756820106 7.4491795045400915 4 2.0840869441589986 4.642822224581672 5 1.1104582821169722 3.0922776555298355 6 0.6869170182260966 2.2954178621975347 7 0.437970334814307 1.7074538167872917 8 0.30900840364162663 1.3767862113725657 9 0.23204938145751092 1.1631324038636783 >10 1.2020083753091506 12.258680793888917 >50 0.08917653811384943 3.4427924985263347 >100 0.045756445907942075 4.36090882275681 >500 0.001970009014797877 0.7784211032164436 >1k 5.516025241433296E-4 0.42811624637061774 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 4.3808404817697896E-5 9 0.0 0.0 0.0 0.0 4.3808404817697896E-5 10-11 0.0 0.0 0.0 0.0 4.3808404817697896E-5 12-13 0.0 0.0 0.0 0.0 4.3808404817697896E-5 14-15 0.0 4.3808404817697896E-5 0.0 2.1904202408848948E-5 4.3808404817697896E-5 16-17 0.0 4.3808404817697896E-5 0.0 4.3808404817697896E-5 4.3808404817697896E-5 18-19 0.0 4.3808404817697896E-5 0.0 4.3808404817697896E-5 4.3808404817697896E-5 20-21 0.0 8.761680963539579E-5 0.0 6.571260722654685E-5 8.761680963539579E-5 22-23 0.0 8.761680963539579E-5 0.0 8.761680963539579E-5 1.0952101204424474E-4 24-25 0.0 8.761680963539579E-5 0.0 8.761680963539579E-5 1.314252144530937E-4 26-27 0.0 8.761680963539579E-5 0.0 1.5332941686194262E-4 1.5332941686194265E-4 28-29 1.314252144530937E-4 8.761680963539579E-5 0.0 7.009344770831663E-4 1.7523361927079158E-4 30-31 2.1904202408848947E-4 8.761680963539579E-5 0.0 0.0021904202408848947 2.1904202408848947E-4 32-33 2.1904202408848947E-4 8.761680963539579E-5 0.0 0.004972253946808711 3.0665883372388525E-4 34-35 2.1904202408848947E-4 8.761680963539579E-5 0.0 0.008652159951495333 3.0665883372388525E-4 36-37 2.1904202408848947E-4 8.761680963539579E-5 0.0 0.015179612269332322 3.0665883372388525E-4 38-39 2.1904202408848947E-4 8.761680963539579E-5 0.0 0.02597838405689485 3.0665883372388525E-4 40-41 2.1904202408848947E-4 8.761680963539579E-5 0.0 0.040150403015420125 3.94275643359281E-4 42-43 2.1904202408848947E-4 8.761680963539579E-5 0.0 0.05395005053299495 4.1617984576813E-4 44-45 2.1904202408848947E-4 1.0952101204424474E-4 0.0 0.0707505737805821 4.3808404817697894E-4 46-47 3.285630361327342E-4 1.314252144530937E-4 0.0 0.09226050054607177 4.3808404817697894E-4 48-49 3.5046723854158316E-4 1.314252144530937E-4 0.0 0.11381423571637914 4.8189245299467686E-4 50-51 3.5046723854158316E-4 1.314252144530937E-4 0.0 0.1371641154842121 4.8189245299467686E-4 52-53 3.5046723854158316E-4 1.314252144530937E-4 0.0 0.16068922887131587 4.8189245299467686E-4 54-55 3.5046723854158316E-4 1.314252144530937E-4 0.0 0.1903694231353062 4.8189245299467686E-4 56-57 3.5046723854158316E-4 1.314252144530937E-4 0.0 0.2225466964739053 5.037966554035259E-4 58-59 3.5046723854158316E-4 1.7523361927079158E-4 0.0 0.2616676019761095 5.476050602212237E-4 60-61 3.94275643359281E-4 1.7523361927079158E-4 0.0 0.306111228663664 5.695092626300726E-4 62-63 4.3808404817697894E-4 1.7523361927079158E-4 0.0 0.35193482010297605 5.695092626300726E-4 64-65 4.3808404817697894E-4 1.7523361927079158E-4 0.0 0.3997735981639021 5.695092626300726E-4 66-67 4.3808404817697894E-4 1.7523361927079158E-4 0.0 0.45273795958849894 5.695092626300726E-4 68-69 4.3808404817697894E-4 1.7523361927079158E-4 0.0 0.5165449012054759 5.695092626300726E-4 70-71 4.3808404817697894E-4 1.7523361927079158E-4 0.0 0.5874488044029199 6.133176674477705E-4 72-73 4.3808404817697894E-4 1.7523361927079158E-4 0.0 0.6607402656629285 8.54263893945109E-4 74-75 4.3808404817697894E-4 1.7523361927079158E-4 0.0 0.7343821941614787 8.761680963539579E-4 76-77 4.3808404817697894E-4 1.7523361927079158E-4 0.0 0.8217142491655594 8.761680963539579E-4 78-79 4.3808404817697894E-4 1.7523361927079158E-4 0.0 0.9198888843620204 8.980722987628068E-4 80-81 5.257008578123748E-4 1.7523361927079158E-4 0.0 1.0306584359435695 9.637849059893537E-4 82-83 5.257008578123748E-4 1.7523361927079158E-4 0.0 1.149751584440481 0.0011828269300778432 84-85 5.476050602212237E-4 1.7523361927079158E-4 0.0 1.2656248151832923 0.0011828269300778432 86-87 5.914134650389215E-4 1.7523361927079158E-4 0.0 1.3917711168558533 0.0011828269300778432 88-89 6.133176674477705E-4 1.7523361927079158E-4 0.0 1.5357474392892174 0.0011828269300778432 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3865 0.0 36.14673 1 GGTATCA 2535 0.0 34.00086 1 TCAACGC 4630 0.0 30.060835 4 ATCAACG 4620 0.0 29.818493 3 TACATGG 7540 0.0 29.761251 2 GTACATG 7785 0.0 29.625578 1 CAACGCA 4740 0.0 29.063593 5 ACATGGG 7520 0.0 28.202969 3 AACGCAG 5020 0.0 27.627495 6 GTACCTG 2225 0.0 27.457195 1 TATCAAC 5020 0.0 27.44312 2 GTGTAGC 1400 0.0 26.385359 1 TACCTGG 2270 0.0 24.817915 2 AGTACAT 5150 0.0 24.268385 2 GAGTACA 6035 0.0 23.855736 1 CATGGGG 4550 0.0 22.994055 4 ACGCAGA 6045 0.0 22.865128 7 CCGTACG 105 0.0022407577 22.542202 9 CGCAGAG 6175 0.0 22.308567 8 TAGCCCT 1505 0.0 21.075262 4 >>END_MODULE