FastQCFastQC Report
Thu 26 May 2016
SRR936317_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936317_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2282667
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23340.10224881684450689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29750.035.3441961
GTATCAA42900.033.160931
GTACCTG24050.029.0813851
ATCAACG50550.027.7771513
TCAACGC50750.027.6676854
TACCTGG24050.027.6219142
TACATGG79350.027.0291462
GTACATG81900.026.5488171
CAACGCA53100.026.443225
ACATGGG79900.025.5886653
AACGCAG55100.025.5694876
TATCAAC55350.025.5469722
AGTACAT56200.021.4455992
ACGCAGA68150.020.5339877
CATGGGG47850.020.5213324
GAGTACA65950.020.3445051
GTGTACG3052.0372681E-1020.2794591
CGCAGAG71400.019.5993188
CATGGGA34750.019.2478164
GTACGCT1559.7146985E-418.3627053
Produced by FastQC (version 0.11.5)