##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936317_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2282667 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.43384733734706 34.0 31.0 34.0 31.0 34.0 2 32.56760885402908 34.0 31.0 34.0 31.0 34.0 3 32.6541637479317 34.0 31.0 34.0 31.0 34.0 4 36.15399267611088 37.0 35.0 37.0 35.0 37.0 5 36.10931993146613 37.0 35.0 37.0 35.0 37.0 6 36.13958540601848 37.0 36.0 37.0 35.0 37.0 7 36.08537513356087 37.0 36.0 37.0 35.0 37.0 8 36.05607169157832 37.0 35.0 37.0 35.0 37.0 9 37.821332677959596 39.0 38.0 39.0 35.0 39.0 10-11 37.74915132167767 39.0 37.5 39.0 35.0 39.0 12-13 37.694090947124565 39.0 37.0 39.0 35.0 39.0 14-15 39.046132221651256 40.0 38.0 41.0 36.0 41.0 16-17 38.95957009936184 40.0 38.0 41.0 35.0 41.0 18-19 38.95461974961744 40.0 38.0 41.0 35.0 41.0 20-21 38.89901001766793 40.0 38.0 41.0 35.0 41.0 22-23 38.77947155673604 40.0 38.0 41.0 34.5 41.0 24-25 38.941004754526176 40.0 38.0 41.0 35.0 41.0 26-27 38.86658960768259 40.0 38.0 41.0 35.0 41.0 28-29 38.78002135221651 40.0 38.0 41.0 35.0 41.0 30-31 38.68231415269945 40.0 38.0 41.0 34.0 41.0 32-33 38.5469974376464 40.0 38.0 41.0 34.0 41.0 34-35 38.42135296125103 40.0 38.0 41.0 34.0 41.0 36-37 38.28657684191343 40.0 38.0 41.0 34.0 41.0 38-39 38.15973289139414 40.0 38.0 41.0 33.0 41.0 40-41 38.024955238762374 40.0 38.0 41.0 33.0 41.0 42-43 38.07990171146295 40.0 38.0 41.0 33.0 41.0 44-45 37.979869599902216 40.0 38.0 41.0 33.0 41.0 46-47 37.82035925520455 40.0 37.0 41.0 33.0 41.0 48-49 37.62481584041825 40.0 37.0 41.0 33.0 41.0 50-51 37.429883990963205 40.0 37.0 41.0 32.0 41.0 52-53 37.215989235398766 40.0 36.0 41.0 32.0 41.0 54-55 37.007614338841364 39.0 36.0 41.0 31.0 41.0 56-57 36.746725869344935 39.0 35.0 41.0 31.0 41.0 58-59 36.463489199256834 39.0 35.0 41.0 31.0 41.0 60-61 36.17187701929366 38.0 35.0 40.0 30.0 41.0 62-63 35.86183836713809 38.0 35.0 40.0 30.0 41.0 64-65 35.47494838274702 37.0 34.5 40.0 29.0 41.0 66-67 35.113805035951366 36.5 34.0 39.5 29.0 41.0 68-69 34.75196557360316 36.0 34.0 39.0 29.0 41.0 70-71 34.37605704204775 35.5 34.0 39.0 28.5 40.5 72-73 33.96297138391189 35.0 34.0 37.5 28.0 40.0 74-75 33.53432936122527 35.0 33.0 37.0 27.5 39.0 76-77 32.821740534208445 35.0 32.5 36.0 26.0 39.0 78-79 32.87827637583581 35.0 33.0 36.0 26.5 39.0 80-81 32.71959291477907 35.0 33.0 36.0 27.0 37.0 82-83 32.43881411524326 35.0 33.0 35.5 26.0 37.0 84-85 32.154759980321266 35.0 33.0 35.0 26.0 36.5 86-87 31.747835974323017 35.0 32.0 35.0 25.0 36.0 88-89 31.26151164405496 34.5 32.0 35.0 24.0 36.0 90-91 31.03814134957048 34.0 32.0 35.0 23.0 35.5 92-93 30.74253515734008 34.0 31.0 35.0 21.0 35.0 94-95 30.570747507192245 34.0 31.0 35.0 20.0 35.0 96-97 30.303128095337602 34.0 31.0 35.0 18.5 35.0 98-99 29.82685275600865 34.0 31.0 35.0 4.5 35.0 100-101 26.406280022447426 31.0 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0716566444886908 1101 2 0.16082539845501032 1101 3 0.1989927311905646 1101 4 0.14812008146179068 1101 5 0.13135694321096025 1101 6 0.19129607908696045 1101 7 0.18067931023634287 1101 8 0.07443746687598463 1101 9 0.2139146157585472 1101 10-11 0.1954806391527555 1101 12-13 0.256771360606308 1101 14-15 0.3499147825581588 1101 16-17 0.36649315442678443 1101 18-19 0.3210029310325382 1101 20-21 0.254890436560153 1101 22-23 0.3213524401761916 1101 24-25 0.2560271433258805 1101 26-27 0.23005091729014282 1101 28-29 0.3197022603897963 1101 30-31 0.3973734843459624 1101 32-33 0.4328432443457544 1101 34-35 0.3832579470113302 1101 36-37 0.3762956363364651 1101 38-39 0.3671817553281471 1101 40-41 0.45979096381540785 1101 42-43 0.3649460994025375 1101 44-45 0.3835507393488342 1101 46-47 0.5393642639249094 1101 48-49 0.47572201648450374 1101 50-51 0.4385116139493661 1101 52-53 0.4921088743242521 1101 54-55 0.5610643383510094 1101 56-57 0.38059244408326265 1101 58-59 0.33287425482905775 1101 60-61 0.583672441315457 1101 62-63 0.5762120148068348 1101 64-65 0.5692692088809821 1101 66-67 0.42503675246691586 1101 68-69 0.5647225510194289 1101 70-71 0.5750071183457877 1101 72-73 0.46044705027224353 1101 74-75 0.3871049028467013 1101 76-77 0.20495650334171955 1101 78-79 0.33850050989451574 1101 80-81 0.4700864634861688 1101 82-83 0.46646617647555644 1101 84-85 0.7442767319494905 1101 86-87 0.6666719829142984 1101 88-89 0.5371806635230243 1101 90-91 0.7504202804064413 1101 92-93 0.9331839979988352 1101 94-95 0.8931286939681939 1101 96-97 0.8584782373613571 1101 98-99 0.8068445186896618 1101 100-101 0.8567814673858685 1102 1 -0.03134366466731109 1102 2 0.07745051189532148 1102 3 0.13863301322061972 1102 4 0.11285236233500484 1102 5 0.13714264823624234 1102 6 0.11571541430182464 1102 7 0.1532586685880446 1102 8 0.06950094358457193 1102 9 0.13556600745920377 1102 10-11 0.20933681180142116 1102 12-13 0.2673514768888623 1102 14-15 0.3301081047884793 1102 16-17 0.32763174607159584 1102 18-19 0.33469604882097315 1102 20-21 0.1656974334586252 1102 22-23 0.1946697838280187 1102 24-25 0.21221898958559393 1102 26-27 0.31295459026293315 1102 28-29 0.14392014556327126 1102 30-31 0.23023153187621404 1102 32-33 0.14798615420184547 1102 34-35 0.1315745427050814 1102 36-37 -0.001371083518336036 1102 38-39 0.07121424662689435 1102 40-41 0.1302688092972346 1102 42-43 0.09169258150639337 1102 44-45 0.10738990841728224 1102 46-47 0.19040741611942025 1102 48-49 0.1928051295857358 1102 50-51 -0.03249129810073015 1102 52-53 -0.010702700077636962 1102 54-55 -0.05466671587096528 1102 56-57 0.11520420397874886 1102 58-59 -0.07401169652375472 1102 60-61 -0.1591930359316649 1102 62-63 -0.0714807838528273 1102 64-65 0.03944662453624659 1102 66-67 0.007910507116719145 1102 68-69 -0.07474869248580518 1102 70-71 -0.07083049496986149 1102 72-73 -0.12785538408196828 1102 74-75 0.03806403441750916 1102 76-77 -0.1252320818207835 1102 78-79 -0.1962306739735169 1102 80-81 -0.04157993723984532 1102 82-83 0.12297864742985354 1102 84-85 0.19665623590369563 1102 86-87 0.07722766589974484 1102 88-89 0.19510428204446129 1102 90-91 0.30186234357399755 1102 92-93 0.19548815764300542 1102 94-95 0.1314186623544984 1102 96-97 0.12603954499152437 1102 98-99 0.2898721033834484 1102 100-101 0.6487915299151616 1103 1 -0.02427059089023942 1103 2 0.039800393023341485 1103 3 0.05967583771054308 1103 4 -0.01973480407811934 1103 5 -0.01136610089419321 1103 6 -0.0070581250253098915 1103 7 0.11378533642686506 1103 8 0.1507524247064822 1103 9 0.06528438407314496 1103 10-11 0.097660220025368 1103 12-13 0.14563717861575753 1103 14-15 0.21109661510472222 1103 16-17 0.20042726333669947 1103 18-19 0.12845937891813008 1103 20-21 0.1262843017362414 1103 22-23 0.13386256283316555 1103 24-25 0.07908285250441338 1103 26-27 0.08282551287765472 1103 28-29 0.02231143694868365 1103 30-31 -0.004796714551901005 1103 32-33 0.07821719010806305 1103 34-35 -0.003207073036470831 1103 36-37 0.00995519903767672 1103 38-39 0.13786234352858173 1103 40-41 0.14031941588832808 1103 42-43 0.14267279753997286 1103 44-45 0.1213568879988216 1103 46-47 0.13905070845274992 1103 48-49 0.01626399835260628 1103 50-51 0.1660516601679447 1103 52-53 0.09172643540584602 1103 54-55 0.11489020889951007 1103 56-57 0.05585612695276865 1103 58-59 0.10373631017283458 1103 60-61 0.0781175715767688 1103 62-63 0.022294299663336403 1103 64-65 0.12138706623546369 1103 66-67 -0.014122375700736711 1103 68-69 0.11712013935812848 1103 70-71 -0.014705052544421449 1103 72-73 0.008448442754811936 1103 74-75 0.21128793036057658 1103 76-77 0.07115226071397984 1103 78-79 -0.034784563877423125 1103 80-81 0.0233839807193732 1103 82-83 0.09984304546368605 1103 84-85 0.05688610602364008 1103 86-87 -0.10187198398214647 1103 88-89 0.010177611912290274 1103 90-91 0.18106156307052856 1103 92-93 0.3284307933137711 1103 94-95 0.17117388545923973 1103 96-97 0.13792578718457804 1103 98-99 0.08704583611643457 1103 100-101 0.5649437854249371 1104 1 -0.013387301395695772 1104 2 -0.040698939366357934 1104 3 0.07146718227195237 1104 4 0.043986402243071154 1104 5 0.007409364284058029 1104 6 0.0797453014678382 1104 7 0.07409428049468403 1104 8 0.07188319800741994 1104 9 0.042303881350171935 1104 10-11 0.09520801767546061 1104 12-13 0.16033761834076188 1104 14-15 0.1571039903360969 1104 16-17 0.16301386643235105 1104 18-19 0.2456797848710366 1104 20-21 0.09470986844092266 1104 22-23 0.2015421538239579 1104 24-25 0.1392261594962747 1104 26-27 0.23160590633793987 1104 28-29 0.27992830695474424 1104 30-31 0.2764092914575542 1104 32-33 0.16555009651463592 1104 34-35 0.06280647687384544 1104 36-37 0.14305176471276582 1104 38-39 0.07574366457559734 1104 40-41 0.13164740642908157 1104 42-43 0.16179114125517202 1104 44-45 0.1716441084668574 1104 46-47 0.2063477845297612 1104 48-49 0.2297106630708754 1104 50-51 0.3367208845747811 1104 52-53 0.2977311316001803 1104 54-55 0.3628740066947742 1104 56-57 0.3256234566452818 1104 58-59 0.4196542313546985 1104 60-61 0.1083428160329305 1104 62-63 0.15958945600258545 1104 64-65 0.16584499341532677 1104 66-67 0.10111621141047067 1104 68-69 0.37311538012762924 1104 70-71 0.2596406368324651 1104 72-73 0.14148042023752794 1104 74-75 0.2743956085475787 1104 76-77 0.2156065585388447 1104 78-79 0.23837740659490692 1104 80-81 0.32160282700473175 1104 82-83 0.272179266044418 1104 84-85 0.4041337178125488 1104 86-87 0.376277030647973 1104 88-89 0.4005728820986647 1104 90-91 0.5201523189123591 1104 92-93 0.5791609622315939 1104 94-95 0.5549762651699979 1104 96-97 0.4687233474939525 1104 98-99 0.2834162662331998 1104 100-101 0.34216681310927655 1105 1 0.039379299265384304 1105 2 0.1405724802138124 1105 3 0.11123432961078095 1105 4 0.06952203840707227 1105 5 0.018573857598809695 1105 6 0.03248009584567768 1105 7 0.06482747689139501 1105 8 0.1355938086903805 1105 9 0.18995729090706703 1105 10-11 0.11729363628676026 1105 12-13 0.107854695341004 1105 14-15 0.03927878735322565 1105 16-17 0.10986089726121406 1105 18-19 0.08192180164496676 1105 20-21 0.14661149618380875 1105 22-23 -0.024012487117026637 1105 24-25 0.1024923418431527 1105 26-27 0.12532575687396275 1105 28-29 0.06334471067939518 1105 30-31 0.1076264266105511 1105 32-33 0.17869452515274986 1105 34-35 0.1331351874926554 1105 36-37 0.12526371165459693 1105 38-39 0.1812164595161434 1105 40-41 0.14623264343468634 1105 42-43 0.23385490901958406 1105 44-45 0.23976426884114943 1105 46-47 0.16354080220257572 1105 48-49 0.20468209201403909 1105 50-51 0.15589565905568747 1105 52-53 0.14004886870277744 1105 54-55 0.08108567112059717 1105 56-57 0.13108180052068974 1105 58-59 0.023956652651577315 1105 60-61 0.25312634163280734 1105 62-63 0.2553701631800891 1105 64-65 0.18826714527185828 1105 66-67 0.14549759314139266 1105 68-69 0.17041549366839348 1105 70-71 0.19209735740252398 1105 72-73 0.16961341464460133 1105 74-75 0.061902685968341586 1105 76-77 -0.08396378945534622 1105 78-79 -0.1345445267531602 1105 80-81 0.0037837111264735768 1105 82-83 0.17312906346232637 1105 84-85 0.1732939831452498 1105 86-87 0.16529856575535362 1105 88-89 0.35674387026110566 1105 90-91 0.31632724742953755 1105 92-93 0.4452258419380932 1105 94-95 0.2483549822824962 1105 96-97 0.2631014184905034 1105 98-99 0.3248500488738486 1105 100-101 0.6903058667410811 1106 1 -0.030298470736909167 1106 2 -0.029901930778478913 1106 3 0.015249099912487907 1106 4 -0.04744229669667277 1106 5 0.004951586421270804 1106 6 -0.035738380289778604 1106 7 -7.037736848118925E-4 1106 8 0.01584522518005116 1106 9 -0.06201938497603976 1106 10-11 0.05876054271546849 1106 12-13 0.027802504951452534 1106 14-15 0.1438252955404664 1106 16-17 0.031391254876020014 1106 18-19 0.173223154608813 1106 20-21 0.19436144416024348 1106 22-23 0.08121065665151406 1106 24-25 0.12113949652742662 1106 26-27 -0.021633674405322267 1106 28-29 0.006088499241585055 1106 30-31 0.09697158385341709 1106 32-33 0.14605204424780993 1106 34-35 0.10992982480721025 1106 36-37 0.052302859714970396 1106 38-39 0.08033805153375795 1106 40-41 0.03656949710227764 1106 42-43 0.11297827027734542 1106 44-45 0.07761099381517766 1106 46-47 0.24419389767042787 1106 48-49 0.1984584714725912 1106 50-51 0.26747434809701787 1106 52-53 0.1894669730782894 1106 54-55 0.15876288708291497 1106 56-57 0.17127197847200648 1106 58-59 0.16170231581810413 1106 60-61 0.17590142335589576 1106 62-63 0.09842154928976043 1106 64-65 0.2613006545926382 1106 66-67 0.18054610895523382 1106 68-69 0.03814273127427015 1106 70-71 0.1375941143809314 1106 72-73 0.03886261585242323 1106 74-75 0.18249352437745614 1106 76-77 0.11089836785864549 1106 78-79 0.1389266609733184 1106 80-81 0.13392789761404345 1106 82-83 0.3029875593711253 1106 84-85 0.2826004278330103 1106 86-87 0.1932419140708852 1106 88-89 0.20171123809711133 1106 90-91 0.3374845939029534 1106 92-93 0.4137072455996744 1106 94-95 0.4676526290613836 1106 96-97 0.37656305319816497 1106 98-99 0.32996262610798865 1106 100-101 0.8325365317558742 1107 1 0.014953210271663409 1107 2 -0.009482702179759883 1107 3 0.030085867802362998 1107 4 0.04064513419110227 1107 5 0.008117193889631835 1107 6 -0.02717381222506532 1107 7 0.06090708066957973 1107 8 -3.120058309207252E-4 1107 9 0.02791134603677392 1107 10-11 0.009798286567750836 1107 12-13 0.10069815516888525 1107 14-15 0.11655201500107637 1107 16-17 0.10140853523837023 1107 18-19 0.11890435497618768 1107 20-21 0.008858506314219028 1107 22-23 0.02544690889686052 1107 24-25 0.0911819505232998 1107 26-27 0.09276296984789667 1107 28-29 0.07201806101750208 1107 30-31 -0.03519506375628367 1107 32-33 0.08950404098722231 1107 34-35 0.06792305282316136 1107 36-37 0.047563208568050186 1107 38-39 0.09460679897489399 1107 40-41 0.1116819024475717 1107 42-43 0.07555024105011654 1107 44-45 -0.030164431554673854 1107 46-47 -0.01600050179548873 1107 48-49 -0.12540447330771087 1107 50-51 0.038062784085916235 1107 52-53 0.1537042765302985 1107 54-55 0.17434379864508287 1107 56-57 0.06775071274540068 1107 58-59 0.011554962461431728 1107 60-61 0.0471724059943881 1107 62-63 0.05249442188719655 1107 64-65 0.047715283701101896 1107 66-67 -0.059256725245496966 1107 68-69 0.06880062067257597 1107 70-71 0.250455443622549 1107 72-73 0.15604680201559518 1107 74-75 0.08315449362689264 1107 76-77 0.009996819818489655 1107 78-79 0.060916521439466464 1107 80-81 0.1961044671393779 1107 82-83 0.36645785079512194 1107 84-85 0.1471238623649711 1107 86-87 0.19058485088413946 1107 88-89 0.24234636979301882 1107 90-91 0.018681272827549833 1107 92-93 0.2171103888920527 1107 94-95 0.14191364921135374 1107 96-97 0.07900886072775393 1107 98-99 0.010107176246858529 1107 100-101 0.18893524522644256 1108 1 -0.11443023634107874 1108 2 -0.03341492114036271 1108 3 -0.013002174341885109 1108 4 -0.05248740630599258 1108 5 -0.0726975565966228 1108 6 -0.020927894231228095 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-0.6221582539215476 2316 76-77 -0.4841458435267967 2316 78-79 -0.5938783565169672 2316 80-81 -0.6869891007575291 2316 82-83 -0.7833654511360884 2316 84-85 -0.8048824762722191 2316 86-87 -0.8029619498294984 2316 88-89 -0.8489835494965838 2316 90-91 -0.7470095436230082 2316 92-93 -0.7194389727036565 2316 94-95 -0.6938315423239025 2316 96-97 -0.6807090091436727 2316 98-99 -0.8474917837528082 2316 100-101 -0.8696849241298743 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 21.0 5 39.0 6 81.0 7 178.0 8 366.0 9 652.0 10 1167.0 11 1665.0 12 2538.0 13 3496.0 14 4443.0 15 5760.0 16 7014.0 17 8892.0 18 8662.0 19 6840.0 20 6921.0 21 7403.0 22 8223.0 23 9313.0 24 11467.0 25 14135.0 26 17605.0 27 22257.0 28 27696.0 29 34700.0 30 43091.0 31 53841.0 32 67659.0 33 87654.0 34 121595.0 35 180057.0 36 306684.0 37 546170.0 38 567071.0 39 97241.0 40 64.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.54093098477348 17.581827269988043 12.071449466461338 24.805792278777137 2 16.974712518775448 18.936297560813166 38.077613358127735 26.011376562283655 3 20.552231227769973 22.260846632469825 27.86267116491367 29.324250974846528 4 13.21519958890193 14.446390997898511 34.95192246613282 37.38648694706674 5 14.649311529014087 36.68323062452824 33.52828073477209 15.139177111685587 6 36.053660038893106 33.746271357144956 16.079261670668565 14.12080693329338 7 31.829040328703222 30.370220448273884 19.02287981558414 18.777859407438754 8 28.358582307450014 34.532018905955184 18.342622905574927 18.766775881019875 9 27.03438565502546 15.167784000031542 18.508393909405093 39.28943643553791 10-11 26.74616762227861 25.33180645703952 26.236541993186886 21.68548392749499 12-13 26.57230761951825 22.04943434513358 27.19966670521811 24.178591330130057 14-15 23.21208043047891 23.993140479973647 24.460335213151986 28.33444387639546 16-17 24.109747712475855 27.11572299518959 25.15495672032571 23.61957257200885 18-19 23.938577111773203 26.10032475170491 26.518475975689842 23.442622160832045 20-21 24.306069239637065 25.512947248316824 26.611530856566212 23.569452655479896 22-23 23.987247362184114 25.312168014141367 26.60096860467918 24.09961601899534 24-25 23.81748979134969 25.49008311928864 26.802345371969 23.89008171739267 26-27 23.89004642914408 25.7740235115994 26.57505548453574 23.76087457472078 28-29 23.676765818188986 25.93726281341486 26.673522830697653 23.712448537698496 30-31 23.841496965799312 25.62519604458689 26.633325214093833 23.89998177551997 32-33 23.726875201384008 25.594360743901305 26.753062186944383 23.9257018677703 34-35 24.056595921035225 25.75395222545143 26.482539062260418 23.706912791252925 36-37 24.156495991199762 25.656474853630545 26.4102208580276 23.77680829714209 38-39 23.834679237934775 25.7075554872144 26.600239938790853 23.85752533605997 40-41 23.952488513340647 25.653473509713283 26.637571802573152 23.75646617437292 42-43 24.021743678082114 25.574954289953823 26.412049972082208 23.991252059881855 44-45 23.855324143287955 25.74542343231569 26.538583448499143 23.860668975897216 46-47 24.01342906746847 25.982748139263588 26.244319208714657 23.759503584553286 48-49 24.23469968962115 25.8727981240569 26.19829282868875 23.6942093576332 50-51 24.03006921255061 25.918584345293432 26.1844511407059 23.866895301450057 52-53 24.162005329641502 25.967884145466574 26.076645633604123 23.7934648912878 54-55 23.99966530290744 25.786461502605952 26.23000086302784 23.983872331458766 56-57 23.897725871321455 26.14738695016079 26.15706884726939 23.797818331248365 58-59 24.027099406693935 25.899520422249218 26.185593566727544 23.887786604329296 60-61 23.998816280566913 25.865123888592812 26.385290161306962 23.75076966953331 62-63 23.976887561786278 25.958254094881116 26.27144038092284 23.79341796240976 64-65 23.854964967229954 25.89244467555999 26.262168704467637 23.990421652742416 66-67 23.834050257878175 26.074696834886563 26.199638405426633 23.89161450180863 68-69 23.697455651656593 25.893417655750927 26.422185101900542 23.98694159069194 70-71 23.77232834698222 25.788743812329844 26.35163862232054 24.087289218367395 72-73 23.782933891049886 26.075475731838686 26.14228431014776 23.999306066963662 74-75 23.782531573812562 26.161196530199106 25.95231805602832 24.103953839960013 76-77 23.917967399832115 26.11755153097894 25.966982865983795 23.997498203205154 78-79 23.840655197523365 26.053209802615868 26.15242700628697 23.953707993573794 80-81 23.757252554493824 26.139555708205048 26.10505624179679 23.998135495504336 82-83 23.977566592060953 26.223973974302865 25.984582946176555 23.813876487459627 84-85 23.8570155303904 26.106349474416508 25.84336606497657 24.193268930216522 86-87 23.810483557034395 25.929205034244347 26.21023038420468 24.05008102451658 88-89 23.865714103795334 26.047756368099588 25.965247626469157 24.12128190163592 90-91 23.806998054142987 26.31859497522416 25.990594145554326 23.88381282507853 92-93 23.791224253480266 26.038840313416696 26.13721415730984 24.032721275793204 94-95 23.775469210889817 26.00473843746345 26.203830791870182 24.015961559776557 96-97 23.79377644986901 25.97477898204695 26.08057845808764 24.150866109996407 98-99 23.640363040077315 25.90820257310372 25.997332881219897 24.454101505599066 100-101 23.611942260092437 26.01780824918406 25.95371607561387 24.416533415109633 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 38.0 1 27.5 2 54.0 3 98.5 4 76.5 5 81.0 6 166.0 7 291.0 8 439.5 9 510.0 10 475.0 11 460.5 12 497.5 13 556.0 14 659.0 15 748.0 16 818.0 17 867.0 18 960.5 19 1129.0 20 1274.0 21 1485.5 22 1852.5 23 2349.0 24 3100.5 25 4139.5 26 5631.5 27 7649.0 28 9710.5 29 12062.0 30 15121.5 31 18618.5 32 22917.5 33 26602.0 34 30124.5 35 34331.5 36 40125.5 37 45472.0 38 49120.5 39 53121.5 40 56970.5 41 61569.5 42 65566.0 43 69958.5 44 75919.5 45 80873.0 46 84440.5 47 86686.0 48 89721.0 49 91536.5 50 90287.5 51 89878.0 52 91014.0 53 92535.5 54 94595.5 55 91584.5 56 83640.5 57 76688.0 58 72017.5 59 66003.0 60 57340.5 61 48936.5 62 40979.5 63 33024.0 64 25123.5 65 18506.0 66 13206.5 67 10603.0 68 8120.5 69 5516.5 70 4164.0 71 3052.0 72 2376.5 73 2077.5 74 1649.5 75 1165.5 76 779.5 77 399.5 78 208.5 79 107.0 80 49.0 81 27.0 82 15.5 83 3.5 84 2.5 85 2.5 86 1.5 87 1.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26201806921465115 2 0.01888142247642779 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.8189245299467686E-4 12-13 1.314252144530937E-4 14-15 0.0 16-17 5.914134650389216E-4 18-19 0.0 20-21 2.1904202408848948E-5 22-23 8.761680963539579E-5 24-25 0.0019275698119787074 26-27 0.002146611836067197 28-29 0.002168516038476046 30-31 0.0010075933108070515 32-33 0.0011171143228512963 34-35 0.001270443739713239 36-37 6.571260722654685E-5 38-39 6.571260722654685E-5 40-41 8.323596915362601E-4 42-43 0.003176109349283097 44-45 0.0038770438263662633 46-47 0.01333965926698901 48-49 0.01292347942122088 50-51 0.00582651784075382 52-53 0.006549356520245835 54-55 2.628504289061874E-4 56-57 0.002475174872199931 58-59 0.0014894857638017285 60-61 0.0013799647517574838 62-63 0.0 64-65 8.104554891274111E-4 66-67 0.0 68-69 0.0 70-71 1.0952101204424475E-4 72-73 0.0011390185252601454 74-75 0.0 76-77 0.1885732785377806 78-79 0.10148216976019718 80-81 0.0010075933108070515 82-83 0.0 84-85 5.914134650389216E-4 86-87 0.12272924609678065 88-89 0.578730931844198 90-91 0.30941876322740025 92-93 0.29156683826418833 94-95 0.002540887479426478 96-97 0.0020589950264318013 98-99 0.0023656538601556864 100-101 7.447428819008642E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2282667.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.82263764544821 #Duplication Level Percentage of deduplicated Percentage of total 1 73.8380885307201 39.003225948907534 2 14.335535885519986 15.144816350682838 3 4.922004644906091 7.799798035412624 4 2.2791640810076212 4.815658335423462 5 1.2411127850473613 3.2779425460844904 6 0.7626412905311899 2.417083472591162 7 0.4966550392445883 1.8364240420961921 8 0.35643423899826965 1.5062237320829346 9 0.2635349368268955 1.2528549427430868 >10 1.345175313908537 13.051648217992224 >50 0.10449779448909867 3.8248321957749325 >100 0.052421291554597044 4.77119063914094 >500 0.002154192375488626 0.8374515418279745 >1k 5.799748703238388E-4 0.46084999923952574 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2334 0.10224881684450689 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 4.3808404817697896E-5 14-15 0.0 0.0 0.0 2.1904202408848948E-5 4.3808404817697896E-5 16-17 0.0 0.0 0.0 4.3808404817697896E-5 4.3808404817697896E-5 18-19 4.3808404817697896E-5 0.0 0.0 8.761680963539579E-5 4.3808404817697896E-5 20-21 8.761680963539579E-5 0.0 0.0 1.5332941686194262E-4 8.761680963539579E-5 22-23 8.761680963539579E-5 0.0 0.0 2.1904202408848947E-4 1.5332941686194262E-4 24-25 1.314252144530937E-4 0.0 0.0 2.1904202408848947E-4 2.1904202408848947E-4 26-27 1.314252144530937E-4 0.0 0.0 2.847546313150363E-4 2.1904202408848947E-4 28-29 1.7523361927079158E-4 0.0 0.0 8.761680963539579E-4 2.1904202408848947E-4 30-31 2.1904202408848947E-4 0.0 0.0 0.0023875580625645353 2.1904202408848947E-4 32-33 2.4094622649733843E-4 0.0 0.0 0.005147487566079503 2.628504289061874E-4 34-35 2.628504289061874E-4 0.0 0.0 0.008871201975583824 2.628504289061874E-4 36-37 2.628504289061874E-4 0.0 0.0 0.015508175305465055 2.628504289061874E-4 38-39 3.5046723854158316E-4 0.0 0.0 0.026482180712298378 2.628504289061874E-4 40-41 3.5046723854158316E-4 0.0 0.0 0.04089514589732099 2.628504289061874E-4 42-43 3.5046723854158316E-4 0.0 0.0 0.05473860181971352 2.628504289061874E-4 44-45 3.5046723854158316E-4 0.0 0.0 0.0717362628889803 2.628504289061874E-4 46-47 3.5046723854158316E-4 0.0 0.0 0.09331190226169653 2.628504289061874E-4 48-49 3.5046723854158316E-4 0.0 0.0 0.11493135003923043 3.0665883372388525E-4 50-51 3.5046723854158316E-4 0.0 0.0 0.13856598443837845 3.0665883372388525E-4 52-53 3.5046723854158316E-4 0.0 0.0 0.16239775665920608 3.94275643359281E-4 54-55 3.5046723854158316E-4 0.0 0.0 0.1920779509231964 4.3808404817697894E-4 56-57 3.5046723854158316E-4 0.0 0.0 0.22473711671479019 4.3808404817697894E-4 58-59 3.5046723854158316E-4 0.0 0.0 0.26427420206276253 4.8189245299467686E-4 60-61 3.5046723854158316E-4 0.0 0.0 0.3087616371551347 5.257008578123748E-4 62-63 3.5046723854158316E-4 0.0 0.0 0.3545633243920379 5.257008578123748E-4 64-65 3.5046723854158316E-4 2.1904202408848948E-5 0.0 0.4026430486794613 5.257008578123748E-4 66-67 3.5046723854158316E-4 8.761680963539579E-5 0.0 0.4560893025570528 5.257008578123748E-4 68-69 3.5046723854158316E-4 1.314252144530937E-4 0.0 0.5203124240197978 5.257008578123748E-4 70-71 3.723714409504321E-4 1.314252144530937E-4 0.0 0.5911068062051976 5.914134650389215E-4 72-73 3.94275643359281E-4 1.314252144530937E-4 0.0 0.6645515968820682 6.133176674477705E-4 74-75 3.94275643359281E-4 1.314252144530937E-4 0.0 0.7393763523106962 6.571260722654684E-4 76-77 3.94275643359281E-4 1.314252144530937E-4 0.0 0.8267522157195947 6.571260722654684E-4 78-79 3.94275643359281E-4 1.314252144530937E-4 0.0 0.924729713094376 6.571260722654684E-4 80-81 5.257008578123748E-4 1.314252144530937E-4 0.0 1.0359154445216934 6.571260722654684E-4 82-83 5.257008578123748E-4 1.314252144530937E-4 0.0 1.155315251852329 7.009344770831663E-4 84-85 5.257008578123748E-4 1.314252144530937E-4 0.0 1.270881823761416 7.009344770831663E-4 86-87 5.257008578123748E-4 1.314252144530937E-4 0.0 1.3969843170291592 7.009344770831663E-4 88-89 5.257008578123748E-4 1.314252144530937E-4 0.0 1.5424282210239162 7.009344770831663E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2975 0.0 35.344196 1 GTATCAA 4290 0.0 33.16093 1 GTACCTG 2405 0.0 29.081385 1 ATCAACG 5055 0.0 27.777151 3 TCAACGC 5075 0.0 27.667685 4 TACCTGG 2405 0.0 27.621914 2 TACATGG 7935 0.0 27.029146 2 GTACATG 8190 0.0 26.548817 1 CAACGCA 5310 0.0 26.44322 5 ACATGGG 7990 0.0 25.588665 3 AACGCAG 5510 0.0 25.569487 6 TATCAAC 5535 0.0 25.546972 2 AGTACAT 5620 0.0 21.445599 2 ACGCAGA 6815 0.0 20.533987 7 CATGGGG 4785 0.0 20.521332 4 GAGTACA 6595 0.0 20.344505 1 GTGTACG 305 2.0372681E-10 20.279459 1 CGCAGAG 7140 0.0 19.599318 8 CATGGGA 3475 0.0 19.247816 4 GTACGCT 155 9.7146985E-4 18.362705 3 >>END_MODULE