FastQCFastQC Report
Thu 26 May 2016
SRR936316_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936316_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2225835
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40700.1828527271787891No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28470.12790705510516276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28550.042.6179241
GTATCAA51600.040.7378921
ATCAACG63300.032.744663
TCAACGC63500.032.7153174
CAACGCA65800.031.6436865
AACGCAG68800.030.4007576
TATCAAC69650.029.7593232
GTACATG87400.029.3598021
TACATGG86000.029.1640242
ACATGGG88000.027.4794673
TACCTGG20850.026.5552182
ACGCAGA81500.025.4892737
GTACCTG22350.025.4196171
CGCAGAG84500.024.6414368
GAGTACA63000.022.6950761
CATGGGG42600.022.5500094
AGTACAT59450.021.8902592
GTATAGC11250.021.8834631
GCAGAGT93000.021.7786469
GTGTAGC15450.021.7568041
Produced by FastQC (version 0.11.5)