##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936316_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2225835 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.21258538930334 31.0 31.0 34.0 28.0 34.0 2 31.304269633643106 33.0 31.0 34.0 28.0 34.0 3 31.372130009636834 33.0 31.0 34.0 28.0 34.0 4 34.86621470144912 37.0 35.0 37.0 32.0 37.0 5 34.83593393041263 37.0 35.0 37.0 32.0 37.0 6 34.916154611640124 37.0 35.0 37.0 32.0 37.0 7 34.85197689855717 37.0 35.0 37.0 32.0 37.0 8 34.84515878310836 37.0 35.0 37.0 32.0 37.0 9 36.452636875599495 39.0 37.0 39.0 32.0 39.0 10-11 36.34692620971455 39.0 37.0 39.0 32.0 39.0 12-13 36.234338574063216 39.0 37.0 39.0 32.0 39.0 14-15 37.38310993402476 40.0 37.5 41.0 32.0 41.0 16-17 37.31940934525695 40.0 37.0 41.0 32.0 41.0 18-19 37.255338109069186 40.0 37.0 41.0 31.0 41.0 20-21 37.163063524475085 40.0 37.0 41.0 31.0 41.0 22-23 36.98559461954727 40.0 37.0 41.0 31.0 41.0 24-25 37.15372792682297 40.0 37.0 41.0 31.0 41.0 26-27 37.09834511542859 40.0 37.0 41.0 31.0 41.0 28-29 36.954393744370094 40.0 37.0 41.0 30.5 41.0 30-31 36.79184306114335 40.0 37.0 41.0 30.0 41.0 32-33 36.602012952442564 40.0 36.0 41.0 30.0 41.0 34-35 36.33038657402727 40.0 36.0 41.0 29.5 41.0 36-37 36.12786122960597 40.0 36.0 41.0 29.0 41.0 38-39 35.9543198395209 40.0 35.0 41.0 28.0 41.0 40-41 35.76376752993821 39.0 35.0 41.0 27.0 41.0 42-43 35.57065999950581 39.0 35.0 41.0 27.0 41.0 44-45 35.39074459697147 39.0 35.0 41.0 26.0 41.0 46-47 35.125518513277044 39.0 35.0 41.0 24.5 41.0 48-49 34.96199561063601 39.0 35.0 40.5 24.0 41.0 50-51 34.67258624291558 38.5 34.5 40.5 24.0 41.0 52-53 34.718141955715495 39.0 34.5 40.0 24.0 41.0 54-55 34.814406952896334 39.0 35.0 41.0 24.0 41.0 56-57 34.66789339730933 39.0 34.0 41.0 23.0 41.0 58-59 34.44032554075212 38.5 34.0 40.5 23.0 41.0 60-61 34.14931228055988 38.0 34.0 40.0 22.0 41.0 62-63 33.856406247543056 37.5 34.0 40.0 21.5 41.0 64-65 33.536590313298156 37.0 33.0 40.0 20.5 41.0 66-67 33.17876392454966 36.5 33.0 39.5 19.5 41.0 68-69 32.79533995107454 36.0 33.0 39.0 18.0 41.0 70-71 32.382256546419654 35.5 32.0 39.0 14.5 40.5 72-73 31.96509602014525 35.0 32.0 38.5 12.0 40.0 74-75 31.525831878823006 35.0 31.5 37.0 7.5 39.5 76-77 31.103578881633183 35.0 31.0 37.0 7.0 39.0 78-79 30.688161970676173 35.0 31.0 36.0 2.0 39.0 80-81 30.261424588974474 35.0 30.5 36.0 2.0 37.5 82-83 29.886877508889924 34.0 30.0 35.5 2.0 37.0 84-85 29.533310645218535 34.0 30.0 35.0 2.0 37.0 86-87 29.179179049660014 34.0 29.5 35.0 2.0 36.0 88-89 28.824770479393127 34.0 29.0 35.0 2.0 36.0 90-91 28.43674576057974 34.0 29.0 35.0 2.0 35.5 92-93 28.085834754148443 34.0 29.0 35.0 2.0 35.0 94-95 27.66665947835307 34.0 27.5 35.0 2.0 35.0 96-97 27.195731040261293 34.0 27.0 35.0 2.0 35.0 98-99 26.632748608949 34.0 25.5 35.0 2.0 35.0 100-101 23.347454101494495 29.0 13.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4106051623116933 1101 2 0.32130802922698365 1101 3 0.40418294642871544 1101 4 0.238608469234876 1101 5 0.44244880648959395 1101 6 0.33494976878794347 1101 7 0.4797378004783681 1101 8 0.43731502813879786 1101 9 0.4916641727205686 1101 10-11 0.6498815937353086 1101 12-13 0.5451426129427333 1101 14-15 0.7203486627065985 1101 16-17 0.7247929595215581 1101 18-19 0.7507633283975537 1101 20-21 0.7179646146344325 1101 22-23 0.719226029914573 1101 24-25 0.7393192174513459 1101 26-27 0.6823899464414254 1101 28-29 0.7136824779129043 1101 30-31 0.9227595711460665 1101 32-33 0.81514574278048 1101 34-35 0.5950521692649176 1101 36-37 0.5522147725158888 1101 38-39 0.5613465804569557 1101 40-41 0.40347261258329326 1101 42-43 0.4917486527106334 1101 44-45 0.4616661852332484 1101 46-47 0.35850860198505785 1101 48-49 0.3799022410297823 1101 50-51 0.37014802661732915 1101 52-53 0.45403535125916505 1101 54-55 0.3515201960694654 1101 56-57 0.26802132512810317 1101 58-59 0.41917736288230145 1101 60-61 0.35406224807275777 1101 62-63 0.36586749192076695 1101 64-65 0.35089799390945586 1101 66-67 0.43303742820732083 1101 68-69 0.4909011746740859 1101 70-71 0.5137023087425021 1101 72-73 0.4613587835443198 1101 74-75 0.5002613582279736 1101 76-77 0.3818311541490509 1101 78-79 0.5484938748998189 1101 80-81 0.6791222757486857 1101 82-83 0.8825141783842803 1101 84-85 0.8018869845237191 1101 86-87 0.7889455639433187 1101 88-89 0.6120429218155259 1101 90-91 0.7322010791557929 1101 92-93 0.7063064884863586 1101 94-95 0.8793505810668307 1101 96-97 0.9334315818110959 1101 98-99 1.0750264554081284 1101 100-101 1.1982157579387973 1102 1 0.5651549136186027 1102 2 0.6259047276993002 1102 3 0.480755993680404 1102 4 0.5023880967027736 1102 5 0.5769074972135115 1102 6 0.4782988146185332 1102 7 0.5539100923459301 1102 8 0.49435424805454886 1102 9 0.6453971067915276 1102 10-11 0.7048763914818466 1102 12-13 0.7673618392872825 1102 14-15 0.8285024019848848 1102 16-17 0.8269686011328119 1102 18-19 0.7915090270221299 1102 20-21 0.6565864827086685 1102 22-23 0.8902252643797155 1102 24-25 0.9168656782272038 1102 26-27 0.8832952654283872 1102 28-29 0.7903945691342074 1102 30-31 0.8292961180340157 1102 32-33 0.6001464935121774 1102 34-35 0.6409784452973284 1102 36-37 0.5886617391368745 1102 38-39 0.7865092566145648 1102 40-41 0.8537717950258994 1102 42-43 0.9506860078511394 1102 44-45 0.9189434716870934 1102 46-47 0.8947972276596516 1102 48-49 1.0212451943025513 1102 50-51 0.880854940962017 1102 52-53 0.8550413317359116 1102 54-55 0.8556648166010277 1102 56-57 1.0504169839956177 1102 58-59 1.0371913286066459 1102 60-61 0.9505648428552789 1102 62-63 1.0033454986540917 1102 64-65 0.9607123305586924 1102 66-67 0.9669147248119003 1102 68-69 0.9746126758837121 1102 70-71 1.2008213917078763 1102 72-73 1.1513166368323127 1102 74-75 1.2793165253767995 1102 76-77 1.3062155541558162 1102 78-79 1.2705642766152891 1102 80-81 1.2300427679749113 1102 82-83 1.2169545766315828 1102 84-85 1.2871084080802007 1102 86-87 1.1873472455823233 1102 88-89 1.110809237012873 1102 90-91 1.195115990590601 1102 92-93 1.3335742797702572 1102 94-95 1.448177874710776 1102 96-97 1.6534253116153579 1102 98-99 1.7242750892879073 1102 100-101 1.5607470031806407 1103 1 0.4126381550567615 1103 2 0.38767315596362906 1103 3 0.537610491620967 1103 4 0.4868520688716629 1103 5 0.3896746701241298 1103 6 0.425577548270212 1103 7 0.5120936857754259 1103 8 0.4223155084953163 1103 9 0.4483065736354206 1103 10-11 0.5316201434305157 1103 12-13 0.5490149062887113 1103 14-15 0.603873828739502 1103 16-17 0.7429829761635816 1103 18-19 0.594540542143811 1103 20-21 0.7289539321253002 1103 22-23 0.69925714462061 1103 24-25 0.7970999083589732 1103 26-27 0.8081596790223031 1103 28-29 0.7124498211074908 1103 30-31 0.6668093484775781 1103 32-33 0.7264959474743335 1103 34-35 0.4437181727296746 1103 36-37 0.6273495047713595 1103 38-39 0.6187987694880945 1103 40-41 0.6790353270004985 1103 42-43 0.5540160284455098 1103 44-45 0.6855084485491929 1103 46-47 0.5820580027790356 1103 48-49 0.6999754101741544 1103 50-51 0.5456447307518104 1103 52-53 0.5547010324202049 1103 54-55 0.6522644387641705 1103 56-57 0.6718601484715734 1103 58-59 0.6069649957163747 1103 60-61 0.5878812063499268 1103 62-63 0.7098337491997171 1103 64-65 0.595659008794307 1103 66-67 0.8898409809861079 1103 68-69 0.6972607850584609 1103 70-71 0.7039420841216 1103 72-73 0.5980620502785499 1103 74-75 0.7254129804384206 1103 76-77 0.899488130010365 1103 78-79 0.7765392577439627 1103 80-81 0.7959465303438371 1103 82-83 0.8874355083830068 1103 84-85 0.9449991369237978 1103 86-87 0.8082078602953935 1103 88-89 0.6927490149055195 1103 90-91 0.8392221869508916 1103 92-93 0.9348422998395094 1103 94-95 0.9928588261540234 1103 96-97 1.0586520034711455 1103 98-99 1.020722151103822 1103 100-101 1.1761100609104993 1104 1 0.21675266560691497 1104 2 0.026646287511017164 1104 3 0.15045551486202768 1104 4 0.1948893140376171 1104 5 0.2688984483578025 1104 6 0.17480202026696645 1104 7 0.2997630382962626 1104 8 0.26538877978640585 1104 9 0.11072741031367883 1104 10-11 0.20789741079688895 1104 12-13 0.19405697806345046 1104 14-15 0.40081259919312373 1104 16-17 0.402715233173069 1104 18-19 0.184353127005906 1104 20-21 0.301400636255174 1104 22-23 0.3111928426949149 1104 24-25 0.2174591289657215 1104 26-27 0.2109510842889577 1104 28-29 0.32007725051709457 1104 30-31 0.3368283774074996 1104 32-33 0.3920679551255475 1104 34-35 0.3234518668198092 1104 36-37 0.42837627411496015 1104 38-39 0.3183988646327407 1104 40-41 0.27987601917782 1104 42-43 0.2980905485101246 1104 44-45 0.3688313754364785 1104 46-47 0.2595518332431652 1104 48-49 0.2520351431744885 1104 50-51 0.1484291983877597 1104 52-53 0.19489890355866635 1104 54-55 0.3790097484302777 1104 56-57 0.5269102480761276 1104 58-59 0.4011358992932159 1104 60-61 0.4609431296593769 1104 62-63 0.6041022751514191 1104 64-65 0.5039150370998442 1104 66-67 0.564315207688054 1104 68-69 0.5163890717610045 1104 70-71 0.4867231324967065 1104 72-73 0.5602772357610561 1104 74-75 0.4530083976378023 1104 76-77 0.5271516846541111 1104 78-79 0.44529291238870883 1104 80-81 0.452125729344818 1104 82-83 0.6702393518304142 1104 84-85 0.5000299102714045 1104 86-87 0.4494394384081595 1104 88-89 0.6192752936595198 1104 90-91 0.6805749258283846 1104 92-93 0.4200968613316931 1104 94-95 0.21123784874096785 1104 96-97 0.5408953854670031 1104 98-99 0.5872376765302363 1104 100-101 0.7781026475565547 1105 1 0.31938682980154454 1105 2 0.32108516719665303 1105 3 0.42647065528657535 1105 4 0.32921242533707584 1105 5 0.36597149223806724 1105 6 0.41118815888082594 1105 7 0.4452238003257776 1105 8 0.46344710701990977 1105 9 0.40884834108379664 1105 10-11 0.5496968290655673 1105 12-13 0.5213824586659896 1105 14-15 0.5021614646244146 1105 16-17 0.6790289443107227 1105 18-19 0.6242416927107257 1105 20-21 0.7130107029038584 1105 22-23 0.7091259628551896 1105 24-25 0.6604330858944891 1105 26-27 0.6472568014365478 1105 28-29 0.5514936314548038 1105 30-31 0.8438616439637272 1105 32-33 0.7437495824322511 1105 34-35 0.471875220828359 1105 36-37 0.43972340590985226 1105 38-39 0.6567839823273829 1105 40-41 0.5856610675270133 1105 42-43 0.6914357925676029 1105 44-45 0.7281135536543033 1105 46-47 0.9058813397346697 1105 48-49 0.8209587475971816 1105 50-51 0.8387829565262166 1105 52-53 0.8991824301603586 1105 54-55 0.797172479100233 1105 56-57 0.939605763736644 1105 58-59 0.8373563793178747 1105 60-61 0.8453081371717914 1105 62-63 0.6937279060024366 1105 64-65 0.7181226689525175 1105 66-67 0.7096897694632958 1105 68-69 0.8364116090341867 1105 70-71 0.9019131583086306 1105 72-73 0.9379128446526863 1105 74-75 0.8951839186918491 1105 76-77 0.7676359470881415 1105 78-79 0.7396003246474407 1105 80-81 0.8663381768541605 1105 82-83 0.9070300056039642 1105 84-85 0.9333128397316663 1105 86-87 0.809416997448114 1105 88-89 0.7003502445398233 1105 90-91 0.764162204789745 1105 92-93 0.6284977743801967 1105 94-95 0.5463671009657993 1105 96-97 0.5878046970207258 1105 98-99 0.6879479858880231 1105 100-101 0.6943593413193128 1106 1 0.21759296577526044 1106 2 0.2243022923045821 1106 3 0.22282452409724485 1106 4 0.24599449660198758 1106 5 0.29323529126399706 1106 6 0.24704310604700908 1106 7 0.23975362236907927 1106 8 0.24234591354012736 1106 9 0.3710549704472115 1106 10-11 0.3870634830237094 1106 12-13 0.41515626378453163 1106 14-15 0.4741814562517419 1106 16-17 0.42749457710170446 1106 18-19 0.43548018495665985 1106 20-21 0.4792979360716103 1106 22-23 0.35459836480332996 1106 24-25 0.3967975887482922 1106 26-27 0.34535393379581336 1106 28-29 0.34167919457318163 1106 30-31 0.4174605760740562 1106 32-33 0.4053505946286933 1106 34-35 0.34338224602487344 1106 36-37 0.4823233547019825 1106 38-39 0.44866393724951337 1106 40-41 0.45749678557722717 1106 42-43 0.44995447409576883 1106 44-45 0.5293263298310578 1106 46-47 0.54638760615763 1106 48-49 0.5383627889073566 1106 50-51 0.5094572464375204 1106 52-53 0.5091324090417828 1106 54-55 0.49365698404721314 1106 56-57 0.4768531818507711 1106 58-59 0.6650367562377042 1106 60-61 0.6943161715745134 1106 62-63 0.6160533693962833 1106 64-65 0.44536390100640233 1106 66-67 0.45463416033260273 1106 68-69 0.5461536260262321 1106 70-71 0.6059875012967026 1106 72-73 0.638553550424227 1106 74-75 0.4513406372018096 1106 76-77 0.5533819373623814 1106 78-79 0.7029238555983 1106 80-81 0.5906475714425738 1106 82-83 0.7251168496666551 1106 84-85 0.6697310734712936 1106 86-87 0.4597268144080111 1106 88-89 0.44504093218303 1106 90-91 0.47571098122066857 1106 92-93 0.4160446837796421 1106 94-95 0.33943450846457424 1106 96-97 0.42322839542505974 1106 98-99 0.43189485118141846 1106 100-101 0.5645936453595013 1107 1 0.2548941515112304 1107 2 0.38228456126205757 1107 3 0.4071166389897094 1107 4 0.39481499362847927 1107 5 0.3713296075042578 1107 6 0.34102276447879376 1107 7 0.3448478139494 1107 8 0.43426868304391064 1107 9 0.5645227898516367 1107 10-11 0.5866480721225642 1107 12-13 0.5407566480304524 1107 14-15 0.6253880910934626 1107 16-17 0.41970626433725755 1107 18-19 0.613430042331025 1107 20-21 0.7262752992939951 1107 22-23 0.7906489180734511 1107 24-25 0.7841541381612913 1107 26-27 0.7127612271353101 1107 28-29 0.7059047263944578 1107 30-31 0.6720726270233044 1107 32-33 0.7556648109943396 1107 34-35 0.710973596091975 1107 36-37 0.7183871072593391 1107 38-39 0.8092372996212731 1107 40-41 0.9796473894446152 1107 42-43 0.4776568448344136 1107 44-45 0.7764914162497192 1107 46-47 0.8102736229793877 1107 48-49 0.8670283409675434 1107 50-51 0.8385521419798252 1107 52-53 0.7399175466443495 1107 54-55 0.817893808592018 1107 56-57 0.6759050330508032 1107 58-59 0.7986505744248618 1107 60-61 0.8552462813485846 1107 62-63 0.9727428414981318 1107 64-65 0.7520123086590544 1107 66-67 0.7984125296339926 1107 68-69 0.8060604243924487 1107 70-71 0.8017402639808466 1107 72-73 0.7308803647762474 1107 74-75 0.7906220826704242 1107 76-77 0.8736252442238133 1107 78-79 0.8707766248820938 1107 80-81 1.0411256789580996 1107 82-83 1.0939771645429879 1107 84-85 1.053653974967947 1107 86-87 0.7978100579128622 1107 88-89 1.1129146003382822 1107 90-91 1.2525561315825477 1107 92-93 0.9774268691416346 1107 94-95 1.1581573307655049 1107 96-97 1.3077881625309686 1107 98-99 1.2088686945938747 1107 100-101 1.2855057161076822 1108 1 -0.02950343758803342 1108 2 -0.010346547179587162 1108 3 -0.07350931986481157 1108 4 0.02852686351992162 1108 5 0.058019135357888274 1108 6 0.049911855129678884 1108 7 0.010665450586898828 1108 8 -0.04646296805992023 1108 9 0.08568777917764692 1108 10-11 0.012179385197448767 1108 12-13 0.05417537325155308 1108 14-15 0.12806636528336668 1108 16-17 0.24834607027325006 1108 18-19 0.12012124636129329 1108 20-21 0.209602696168389 1108 22-23 0.22439023745726416 1108 24-25 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-1.709187601265885 2315 98-99 -1.6667882117418067 2315 100-101 -1.8719138600746383 2316 1 -1.1929555980073907 2316 2 -1.2462180010706945 2316 3 -1.3070393250417673 2316 4 -1.1024325871514193 2316 5 -1.3394365724424446 2316 6 -1.232200789809525 2316 7 -1.3355812227175647 2316 8 -1.6324954919561563 2316 9 -1.6955417957242531 2316 10-11 -1.654541529544936 2316 12-13 -1.6790137143663912 2316 14-15 -1.962308877109514 2316 16-17 -2.0257227586347213 2316 18-19 -1.9801242733713806 2316 20-21 -1.9708273884848708 2316 22-23 -1.9303808356632217 2316 24-25 -1.977541794700386 2316 26-27 -1.9057248190387241 2316 28-29 -2.269300766940191 2316 30-31 -2.0813570094842575 2316 32-33 -2.0427679342760925 2316 34-35 -1.996451006137022 2316 36-37 -2.0565001144926853 2316 38-39 -1.9153591774793313 2316 40-41 -1.976828286882494 2316 42-43 -2.1161883524693152 2316 44-45 -2.2143200667260388 2316 46-47 -2.1735342417798407 2316 48-49 -2.2081897517584963 2316 50-51 -2.285946278987751 2316 52-53 -2.3488925503983538 2316 54-55 -2.36027528071849 2316 56-57 -2.387339255453348 2316 58-59 -2.4314323898284798 2316 60-61 -2.541646599173575 2316 62-63 -2.5412868547195906 2316 64-65 -2.325569539452541 2316 66-67 -2.532386682630147 2316 68-69 -2.603685478751096 2316 70-71 -2.416849590222281 2316 72-73 -2.2527661729931623 2316 74-75 -2.2796833430109693 2316 76-77 -2.6390900067916725 2316 78-79 -2.7830523575527124 2316 80-81 -2.3675514671154083 2316 82-83 -2.552959676899153 2316 84-85 -2.6784238016229303 2316 86-87 -2.7229748293910596 2316 88-89 -2.742370981301665 2316 90-91 -2.892630763954802 2316 92-93 -2.946897351544923 2316 94-95 -2.902252916374298 2316 96-97 -3.0190731921227254 2316 98-99 -2.8917837347605158 2316 100-101 -2.902130720236631 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33192.0 3 7028.0 4 5331.0 5 4309.0 6 4008.0 7 4382.0 8 4984.0 9 5982.0 10 6878.0 11 7983.0 12 9690.0 13 12862.0 14 12772.0 15 11973.0 16 13563.0 17 15910.0 18 14838.0 19 12033.0 20 12061.0 21 12524.0 22 13494.0 23 14684.0 24 16519.0 25 19216.0 26 22468.0 27 26810.0 28 31794.0 29 38308.0 30 45790.0 31 56311.0 32 70274.0 33 90876.0 34 123519.0 35 179735.0 36 282592.0 37 451368.0 38 446736.0 39 82937.0 40 101.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.63130840953467 18.026679156133923 12.061220563752018 24.280791870579392 2 15.99510595525984 19.274232755591623 39.85343926612694 24.8772220230216 3 20.350008829075833 23.374405385331873 28.0442451274959 28.2313406580964 4 12.4545875248797 14.84281420310822 36.73344685516082 35.969151416851254 5 13.89183061583662 37.53191779978694 33.95281085566887 14.623440728707576 6 34.278652632449166 34.83173630983109 16.97158156321845 13.918029494501289 7 30.42480826343055 31.35911686607869 19.76641161696717 18.449663253523596 8 27.398669763333256 35.04759799408614 19.13144163488899 18.422290607691615 9 26.981896709379033 15.559196174175144 19.454308613722628 38.0045985027232 10-11 25.910902525713375 26.13549724209909 26.99402503474052 20.959575197447013 12-13 26.190246186251816 22.978686503429138 27.729853812310388 23.101213498008654 14-15 22.82242411171618 24.762338024118904 25.516615445444906 26.89862241872001 16-17 23.02633796152545 28.000255232834803 26.08657066377163 22.886836141868116 18-19 22.82920974922402 26.880595190809377 27.97265152509108 22.31754353487553 20-21 23.4611004942369 26.108652144699697 28.06428112354709 22.365966237516314 22-23 22.934103755358056 26.227298460653657 27.81604676449708 23.022551019491196 24-25 22.728912906910594 26.23844796766015 28.26739511612921 22.76524400930004 26-27 22.725960558851817 26.620449882406223 27.914823869930434 22.73876568881153 28-29 22.53098832363111 26.66258506763846 28.070040239323994 22.736386369406432 30-31 22.75562687022201 26.522255964883467 28.009738915378513 22.712378249516007 32-33 22.479224283381 26.492492883797524 28.10155219709241 22.926730635729065 34-35 22.913637207257366 26.657778750808607 27.899271488214616 22.52931255371941 36-37 22.98676781861985 26.45030133874664 27.740724540137347 22.822206302496163 38-39 22.729672298940464 26.75069373678966 27.856113516667175 22.6635204476027 40-41 23.012000977017955 26.59188686818339 27.853206212642267 22.542905942156384 42-43 22.81719160247173 26.44831359632491 27.859405867290736 22.875088933912625 44-45 22.88690757039352 26.766176072862596 27.708202866264614 22.63871349047927 46-47 22.84053398041813 26.90047481532639 27.313361634262378 22.9456295699931 48-49 22.96393818981756 26.821486396751936 27.396856024831006 22.817719388599496 50-51 22.88911885930259 27.021405689261297 27.234882261596187 22.854593189839925 52-53 23.075257508738282 26.986640319543163 27.187946552246938 22.750155619471617 54-55 22.8970080617306 26.867461175689737 27.34035132078283 22.895179441796834 56-57 22.904310976909155 27.1734350563275 27.145194554707565 22.777059412055785 58-59 22.98147568334956 26.913680129176527 27.228359421540905 22.876484765933007 60-61 22.98364268900613 26.941211737607638 27.38111469169322 22.69403088169301 62-63 22.86631639869873 26.982943171896455 27.433087772553954 22.717652656850856 64-65 22.75163033638006 26.938715245716516 27.301694520363007 23.00795989754042 66-67 22.80017948045464 27.202980386704546 27.059973827271733 22.936866305569083 68-69 22.90409032816497 26.904949125726475 27.325610928131244 22.865349617977316 70-71 22.76524430915165 26.83054389763775 27.340021177244417 23.06419061596618 72-73 22.88203966384248 26.972175894865448 27.209972938001204 22.935811503290875 74-75 22.74483578876167 27.083283475201352 27.113424127061247 23.058456608975728 76-77 22.81848211788433 27.073217697254766 27.145252275658336 22.96304790920257 78-79 22.844625780559724 27.00288543543618 27.149798389736503 23.002690394267596 80-81 22.697608353054342 27.2016739700057 27.09117009717794 23.00954757976201 82-83 22.981549582252566 27.200471362685143 27.007050122920013 22.810928932142286 84-85 22.945325852644764 27.0834683118583 26.913728348786165 23.057477486710777 86-87 22.756673777837978 26.940300023703905 27.235108868646506 23.06791732981161 88-89 22.947326022162905 26.95083643158348 27.091225714945633 23.010611831307983 90-91 22.755208552111267 27.267149141170993 27.078463759985873 22.899178546731868 92-93 22.826143408305818 27.035933592830897 27.193343747558462 22.944579251304827 94-95 22.73845320330489 27.05203395187142 27.20653302632173 23.002979818501963 96-97 22.777695026337526 27.004405385764645 27.133136535362702 23.08476305253513 98-99 22.629016104813303 26.877838020321594 27.078431699984613 23.414714174880498 100-101 22.74735056098731 27.12603967828031 26.812923132754285 23.313686627978093 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 80.0 1 75.0 2 118.0 3 187.0 4 163.0 5 177.5 6 371.0 7 654.5 8 989.0 9 1162.0 10 1145.5 11 1211.0 12 1431.0 13 1702.0 14 1927.5 15 2154.5 16 2378.5 17 2510.5 18 2617.5 19 2768.5 20 2904.0 21 3099.5 22 3545.0 23 4301.0 24 5339.0 25 6610.0 26 8644.5 27 11355.0 28 13807.5 29 16548.5 30 20367.5 31 24509.5 32 29135.0 33 33870.0 34 38872.0 35 44146.0 36 49961.0 37 55634.0 38 59392.5 39 62628.5 40 66126.5 41 70422.0 42 74469.0 43 78111.5 44 81920.5 45 85039.0 46 86225.5 47 86465.5 48 88990.0 49 90296.5 50 88220.5 51 85072.5 52 82310.0 53 80326.0 54 77545.0 55 72295.0 56 65266.0 57 58217.5 58 52305.0 59 46794.0 60 40849.5 61 34587.0 62 28359.0 63 21786.5 64 15974.0 65 12071.0 66 8759.5 67 6865.5 68 5244.0 69 3666.0 70 2913.5 71 2248.0 72 1821.0 73 1628.0 74 1299.0 75 1001.5 76 674.0 77 350.0 78 192.5 79 123.5 80 85.5 81 52.0 82 45.5 83 34.5 84 23.0 85 19.5 86 19.5 87 17.5 88 18.5 89 17.0 90 20.0 91 22.5 92 16.5 93 14.0 94 19.0 95 26.5 96 29.0 97 26.0 98 18.5 99 14.5 100 9.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04613998791464776 2 0.012265060078577254 3 0.010512908638780502 4 0.0056158699993485595 5 0.008805684158978542 6 0.005930358719312079 7 0.0056158699993485595 8 0.00970422335887431 9 0.014736042878290619 10-11 0.0021564940797498466 12-13 0.00912017287894206 14-15 0.008760757198983752 16-17 0.018689615357832003 18-19 0.011815790478629368 20-21 0.0036166202795804717 22-23 0.009659296398879521 24-25 0.021070744237555793 26-27 0.007390484919142703 28-29 0.01797078399791539 30-31 0.01484836027827759 32-33 0.012579548798540773 34-35 0.2680791702889028 36-37 0.4741591357849975 38-39 0.47072222334539626 40-41 0.49114152666302757 42-43 0.48103296066420015 44-45 0.49581393050248557 46-47 0.5024855840617116 48-49 0.5097188246208726 50-51 0.5184795818198563 52-53 0.5076072575011176 54-55 0.49644290794241264 56-57 0.49109659970303277 58-59 0.4888727151832908 60-61 0.4852560949037103 62-63 0.48381843218387705 64-65 0.49904867162211036 66-67 0.47406928186500796 68-69 0.4991609890220973 70-71 0.48168440158412457 72-73 0.4906473301030849 74-75 0.503990637221537 76-77 0.49089442838305625 78-79 0.49051254922310056 80-81 0.49332048422277486 82-83 0.49329802074277745 84-85 0.4931407763827957 86-87 0.4946458295426211 88-89 0.5067087183012218 90-91 0.5315757906583372 92-93 0.5191759496997757 94-95 0.49127630754301194 96-97 0.46566794034598247 98-99 0.45654776746704046 100-101 0.4657128673059773 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2225835.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.740584634242786 #Duplication Level Percentage of deduplicated Percentage of total 1 75.64749381288702 44.435780126842474 2 13.713336385481503 16.110587931384345 3 4.769732632056329 8.405306500680434 4 2.1520112793645065 5.056416027574235 5 1.0869013976632114 3.192261176925632 6 0.6947713374658098 2.448676472991387 7 0.4117545885614582 1.6930693680552507 8 0.2921602011162858 1.3729328816342863 9 0.1973421384996492 1.0432793329596994 >10 0.9408274638848259 9.801860139807227 >50 0.06196375235473151 2.5139738950406447 >100 0.030401582385434816 3.1004496882621435 >500 0.001073411524160436 0.4250210477796944 >1k 2.3001675517713858E-4 0.4003854100625469 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4070 0.1828527271787891 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2847 0.12790705510516276 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.492695999478847E-5 0.0 5 0.0 0.0 0.0 4.492695999478847E-5 0.0 6 0.0 0.0 0.0 4.492695999478847E-5 0.0 7 0.0 0.0 0.0 4.492695999478847E-5 0.0 8 0.0 0.0 0.0 4.492695999478847E-5 0.0 9 0.0 0.0 0.0 4.492695999478847E-5 0.0 10-11 0.0 0.0 0.0 8.985391998957695E-5 0.0 12-13 0.0 0.0 0.0 8.985391998957695E-5 0.0 14-15 0.0 0.0 0.0 2.0217131997654812E-4 0.0 16-17 0.0 0.0 0.0 3.594156799583078E-4 0.0 18-19 0.0 0.0 0.0 4.941965599426732E-4 0.0 20-21 0.0 0.0 0.0 7.412948399140099E-4 0.0 22-23 0.0 0.0 0.0 0.0014151992398358368 0.0 24-25 0.0 0.0 0.0 0.0019543227597732986 0.0 26-27 0.0 0.0 0.0 0.002291274959734212 0.0 28-29 0.0 0.0 0.0 0.002852861959669068 0.0 30-31 0.0 0.0 0.0 0.00487457515943455 0.0 32-33 0.0 0.0 0.0 0.007502802319129675 0.0 34-35 0.0 0.0 0.0 0.012714329678525139 0.0 36-37 0.0 0.0 0.0 0.02120552511754016 0.0 38-39 0.0 0.0 0.0 0.034503905275997546 0.0 40-41 0.0 0.0 0.0 0.05110441699407189 0.0 42-43 0.0 0.0 0.0 0.06680638951225046 0.0 44-45 0.0 0.0 0.0 0.08587788403003817 0.0 46-47 0.0 0.0 0.0 0.10995873458724478 0.0 48-49 0.0 0.0 0.0 0.13729678974407356 0.0 50-51 0.0 0.0 0.0 0.1676224877405558 0.0 52-53 0.0 0.0 0.0 0.1972293543771214 0.0 54-55 0.0 0.0 0.0 0.2300035716933196 0.0 56-57 0.0 0.0 0.0 0.26868568424883243 0.0 58-59 0.0 0.0 0.0 0.3117257119238398 0.0 60-61 0.0 0.0 0.0 0.36415547423775796 0.0 62-63 0.0 0.0 0.0 0.41539467211181424 0.0 64-65 0.0 0.0 0.0 0.4666787969458652 0.0 66-67 0.0 0.0 0.0 0.5282287321387256 0.0 68-69 0.0 0.0 0.0 0.6045596371698712 0.0 70-71 0.0 0.0 0.0 0.6852709208005086 0.0 72-73 0.0 0.0 0.0 0.767914063710922 0.0 74-75 0.0 0.0 0.0 0.8539267286209444 0.0 76-77 0.0 0.0 0.0 0.9471501706101306 0.0 78-79 0.0 0.0 0.0 1.0599617671570445 0.0 80-81 0.0 0.0 0.0 1.1878912857422046 0.0 82-83 0.0 0.0 0.0 1.3223576770066066 0.0 84-85 0.0 0.0 0.0 1.460103736350628 0.0 86-87 0.0 0.0 0.0 1.6058243310937244 0.0 88-89 0.0 0.0 0.0 1.7712004708345406 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2855 0.0 42.617924 1 GTATCAA 5160 0.0 40.737892 1 ATCAACG 6330 0.0 32.74466 3 TCAACGC 6350 0.0 32.715317 4 CAACGCA 6580 0.0 31.643686 5 AACGCAG 6880 0.0 30.400757 6 TATCAAC 6965 0.0 29.759323 2 GTACATG 8740 0.0 29.359802 1 TACATGG 8600 0.0 29.164024 2 ACATGGG 8800 0.0 27.479467 3 TACCTGG 2085 0.0 26.555218 2 ACGCAGA 8150 0.0 25.489273 7 GTACCTG 2235 0.0 25.419617 1 CGCAGAG 8450 0.0 24.641436 8 GAGTACA 6300 0.0 22.695076 1 CATGGGG 4260 0.0 22.550009 4 AGTACAT 5945 0.0 21.890259 2 GTATAGC 1125 0.0 21.883463 1 GCAGAGT 9300 0.0 21.778646 9 GTGTAGC 1545 0.0 21.756804 1 >>END_MODULE