FastQCFastQC Report
Thu 26 May 2016
SRR936316_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936316_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2225835
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39860.17907886253922686No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30180.1355895652642716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29650.045.097831
GTATCAA51500.037.6987531
CGTATAC1404.620233E-1033.884993
ATCAACG60750.031.4698143
TCAACGC61400.031.0594024
CAACGCA62550.030.5642095
TACATGG84300.030.2822192
GTACATG87150.030.085571
AACGCAG65750.029.004526
ACATGGG88100.028.1618523
TATCAAC68650.027.9237562
GTACCTG22550.026.5887581
TACCTGG21150.026.4730872
ACGCAGA78750.024.2767147
CGCAGAG81400.023.4280998
GAGTACA62850.022.7138121
GTACGCT1050.002213332822.5899963
AGTACAT57000.022.476152
CATGGGG39350.022.18244
CATGGGA50000.021.5373024
Produced by FastQC (version 0.11.5)