FastQCFastQC Report
Thu 26 May 2016
SRR936315_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936315_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488692
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31700.21293860650826363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21780.14630292901419503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19250.049.4367521
GTATCAA34200.045.9617541
TCAACGC42700.036.1362424
ATCAACG43300.035.635513
CAACGCA43550.035.4309435
TATCAAC45600.034.0468442
AACGCAG46000.033.7485166
TATATCG902.4726543E-531.5545525
ACGCAGA52750.029.4309757
CGCAGAG53900.028.716198
GTACCTG13550.026.2063521
GTGGTAT9100.026.0143641
GTGTAGC8600.025.8752161
GTACATG49200.024.9240861
TACATGG48800.024.8306452
TACCTGG15000.023.9822652
ACATGGG49650.023.7374113
GCAGAGT63050.023.4977959
TACCCGG2103.5786798E-822.5397222
CATGGGG34500.021.8137974
Produced by FastQC (version 0.11.5)