FastQCFastQC Report
Thu 26 May 2016
SRR936315_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936315_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488692
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32360.21737202859960286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23540.15812538792443298No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16080.10801428367990155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19100.045.076971
GTATCAA31950.042.8776661
ATCAACG41250.033.1192363
TCAACGC41700.032.8755844
TATCAAC42300.032.416892
CAACGCA42600.032.1810345
AACGCAG44350.031.2320866
ATACCGT1004.9995277E-528.4618426
GTACCTG13500.025.7216381
ACGCAGA54600.025.02147
CGCAGAG56300.024.0973558
GTACATG49250.023.4698091
TACCTGG15300.022.9484922
TACATGG48900.022.8019962
GTGGTAT10600.022.4374581
ACATGGG50350.021.95173
GTGTAGC9450.020.6377121
TATCACG1150.00374769520.6293722
GCAGAGT65100.020.4027549
CTACACG2801.6025297E-920.3298874
Produced by FastQC (version 0.11.5)