##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936315_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1488692 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.44720466019835 34.0 31.0 34.0 31.0 34.0 2 32.58926561034787 34.0 31.0 34.0 31.0 34.0 3 32.671058217549366 34.0 31.0 34.0 31.0 34.0 4 36.16510131041209 37.0 35.0 37.0 35.0 37.0 5 36.12248067431007 37.0 35.0 37.0 35.0 37.0 6 36.15795409661636 37.0 36.0 37.0 35.0 37.0 7 36.10960292659596 37.0 36.0 37.0 35.0 37.0 8 36.08716443696883 37.0 36.0 37.0 35.0 37.0 9 37.85017250042319 39.0 38.0 39.0 35.0 39.0 10-11 37.78173053929221 39.0 38.0 39.0 35.0 39.0 12-13 37.721054791723205 39.0 38.0 39.0 35.0 39.0 14-15 39.06429133763062 40.0 38.0 41.0 36.0 41.0 16-17 38.98288497553557 40.0 38.0 41.0 35.0 41.0 18-19 38.99584299505875 40.0 38.5 41.0 35.0 41.0 20-21 38.94449993685732 40.0 38.0 41.0 35.0 41.0 22-23 38.82366601016194 40.0 38.0 41.0 35.0 41.0 24-25 38.98787056019647 40.0 38.0 41.0 35.0 41.0 26-27 38.90399525220798 40.0 38.0 41.0 35.0 41.0 28-29 38.8107194100593 40.0 38.0 41.0 35.0 41.0 30-31 38.700709414707674 40.0 38.0 41.0 35.0 41.0 32-33 38.55709105711591 40.0 38.0 41.0 34.0 41.0 34-35 38.420757953962266 40.0 38.0 41.0 34.0 41.0 36-37 38.27350788477402 40.0 38.0 41.0 34.0 41.0 38-39 38.14482344232387 40.0 38.0 41.0 33.0 41.0 40-41 38.00037516155122 40.0 38.0 41.0 33.0 41.0 42-43 38.028703385253635 40.0 38.0 41.0 33.0 41.0 44-45 37.902240020098176 40.0 38.0 41.0 33.0 41.0 46-47 37.7069820352363 40.0 37.0 41.0 33.0 41.0 48-49 37.48274357624008 40.0 37.0 41.0 32.5 41.0 50-51 37.276045683056 40.0 37.0 41.0 32.0 41.0 52-53 37.055636088593204 40.0 36.0 41.0 31.0 41.0 54-55 36.85158682924339 39.0 36.0 41.0 31.0 41.0 56-57 36.58072186859337 39.0 35.0 41.0 31.0 41.0 58-59 36.306577519057 39.0 35.0 41.0 30.5 41.0 60-61 36.022089525570095 38.0 35.0 40.0 30.0 41.0 62-63 35.7050511455694 38.0 35.0 40.0 29.5 41.0 64-65 35.311272916090104 37.0 34.5 40.0 29.0 41.0 66-67 34.946450978442826 37.0 34.0 39.5 28.5 41.0 68-69 34.579580262404846 36.0 34.0 39.0 28.0 41.0 70-71 34.191741475066706 36.0 34.0 39.0 28.0 40.5 72-73 33.772976209988364 35.0 33.5 38.0 27.5 40.0 74-75 33.33820629116029 35.0 33.0 37.0 26.0 39.0 76-77 32.629249703766796 35.0 32.5 36.0 26.0 39.0 78-79 32.68678443895715 35.0 33.0 36.0 26.0 39.0 80-81 32.51287506079162 35.0 33.0 36.0 26.0 37.5 82-83 32.224414788283944 35.0 33.0 35.5 26.0 37.0 84-85 31.922876256472122 35.0 32.5 35.0 25.5 37.0 86-87 31.502735622949544 35.0 32.0 35.0 24.5 36.0 88-89 31.001455976118635 34.5 31.5 35.0 22.0 36.0 90-91 30.770335301056228 34.0 31.0 35.0 20.0 35.5 92-93 30.45990104064508 34.0 31.0 35.0 19.5 35.0 94-95 30.27360763677107 34.0 31.0 35.0 18.0 35.0 96-97 29.979374847181283 34.0 31.0 35.0 12.0 35.0 98-99 29.472145682249923 34.0 31.0 35.0 2.0 35.0 100-101 25.990560169598545 30.5 24.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04775365850245805 1101 2 -0.04925534094969919 1101 3 0.05497935389489328 1101 4 0.11306841831702741 1101 5 0.05968018260440999 1101 6 0.18203021216817916 1101 7 0.07647720146728432 1101 8 0.039203070630826176 1101 9 0.07117224982433612 1101 10-11 0.09293474144595848 1101 12-13 0.19349945344354325 1101 14-15 0.20209787556580494 1101 16-17 0.21876754889240857 1101 18-19 0.11564017226806556 1101 20-21 0.08737217092328109 1101 22-23 0.11178473583218107 1101 24-25 -0.0021904743452765274 1101 26-27 0.11949720444533085 1101 28-29 0.19927456492207085 1101 30-31 0.18478834354111484 1101 32-33 0.22958599304849514 1101 34-35 0.10474438243397088 1101 36-37 0.0363189868423035 1101 38-39 0.2751045081589467 1101 40-41 0.14321089982178847 1101 42-43 0.11785156531605878 1101 44-45 0.19250263570550175 1101 46-47 0.18395738783766546 1101 48-49 0.2916897835739647 1101 50-51 0.1712506840861181 1101 52-53 0.12669117417998876 1101 54-55 0.06258920360914288 1101 56-57 0.017185642838725812 1101 58-59 -0.05891294005339631 1101 60-61 0.04243166107789165 1101 62-63 -0.09248005682131577 1101 64-65 -0.05661686833285984 1101 66-67 -0.05742897167718297 1101 68-69 -0.015975568357440295 1101 70-71 0.041200381382914486 1101 72-73 -0.09089490099600539 1101 74-75 -0.2227321143189016 1101 76-77 -0.1146364976432821 1101 78-79 -0.1977693298463663 1101 80-81 -0.09789189577155355 1101 82-83 -0.21935444510992141 1101 84-85 -0.10200092839331276 1101 86-87 -0.2567342600013838 1101 88-89 -0.23189756926154814 1101 90-91 -0.009076387833573563 1101 92-93 0.045745225584887805 1101 94-95 0.15517978302577262 1101 96-97 -0.012051052122572514 1101 98-99 -0.08520723041821299 1101 100-101 -0.11882793558395477 1102 1 0.08078827053235926 1102 2 0.0839100167894955 1102 3 0.14655546019004362 1102 4 0.11271023506483147 1102 5 0.02530164819146563 1102 6 0.13083412124727545 1102 7 0.1582259120958014 1102 8 0.16920594668354028 1102 9 0.06694286432917096 1102 10-11 0.18877287555235256 1102 12-13 0.08570188050266836 1102 14-15 0.19579261521245428 1102 16-17 0.32878097635324366 1102 18-19 0.2485961486644328 1102 20-21 0.2007192316738724 1102 22-23 0.12301133996675873 1102 24-25 0.14483110546048295 1102 26-27 0.22178351931605533 1102 28-29 0.1628839699085276 1102 30-31 0.133798399138918 1102 32-33 0.18072662143953977 1102 34-35 0.14332583559930612 1102 36-37 0.21675071235041798 1102 38-39 0.11993205380379379 1102 40-41 0.13321676367569069 1102 42-43 0.15163959729024157 1102 44-45 0.2563954112845792 1102 46-47 0.20189566386461877 1102 48-49 0.16902025379388164 1102 50-51 0.006158788614463617 1102 52-53 0.061216863682744815 1102 54-55 -0.00715919177105917 1102 56-57 0.18961264903254005 1102 58-59 0.1656273767358698 1102 60-61 0.10153689696420543 1102 62-63 -0.03080166538759954 1102 64-65 0.07381329857818741 1102 66-67 0.06284678825652179 1102 68-69 -0.0919724676314857 1102 70-71 -0.11611146801308792 1102 72-73 4.4182831450712E-4 1102 74-75 -0.07604754437112149 1102 76-77 -0.09326166210467335 1102 78-79 0.014088417880877557 1102 80-81 0.10335386440328875 1102 82-83 0.03929709199027798 1102 84-85 0.0717167726230663 1102 86-87 0.015415315913177352 1102 88-89 0.13546258488075935 1102 90-91 0.28864976607229664 1102 92-93 0.2155558472180985 1102 94-95 0.1724119851705197 1102 96-97 0.13325577682639533 1102 98-99 0.23007137321996396 1102 100-101 0.5362583548197364 1103 1 0.06485859593608012 1103 2 0.13728532976371355 1103 3 0.15882505581449635 1103 4 -0.023348420132919045 1103 5 0.0783862124541983 1103 6 0.1479446829178741 1103 7 0.10758699180232156 1103 8 0.1800301857326545 1103 9 0.106286197181646 1103 10-11 0.16878613816665222 1103 12-13 0.13123452655077728 1103 14-15 0.2918545916551025 1103 16-17 0.3098934927497936 1103 18-19 0.06369450559873968 1103 20-21 0.15111498540077406 1103 22-23 0.20237679208658932 1103 24-25 0.26109368733140315 1103 26-27 0.14282455626015178 1103 28-29 0.16695696796386272 1103 30-31 0.19660431430962433 1103 32-33 0.14378951374062865 1103 34-35 0.2457149513094592 1103 36-37 0.31219456821510505 1103 38-39 0.23050378877962885 1103 40-41 0.2764845890878007 1103 42-43 0.3585738422326017 1103 44-45 0.27155935441860635 1103 46-47 0.3418920071404372 1103 48-49 0.5444444953031891 1103 50-51 0.5821336634527299 1103 52-53 0.503521136682366 1103 54-55 0.5384578832298175 1103 56-57 0.527644649283026 1103 58-59 0.46445890239500187 1103 60-61 0.5517464596893262 1103 62-63 0.46421926292830307 1103 64-65 0.4791690016700727 1103 66-67 0.3920587586046267 1103 68-69 0.348038524906336 1103 70-71 0.2687397413338175 1103 72-73 0.40047990521306787 1103 74-75 0.33519526099610175 1103 76-77 0.1821402700323418 1103 78-79 0.14520154932277762 1103 80-81 0.1918505034600102 1103 82-83 0.3997871936740509 1103 84-85 0.37572865753578455 1103 86-87 -0.004335188144448665 1103 88-89 0.21758959062752226 1103 90-91 0.3481814034460342 1103 92-93 0.5387473622760872 1103 94-95 0.45583550651762295 1103 96-97 0.39160334007262776 1103 98-99 0.10583640576704667 1103 100-101 0.24510259514367405 1104 1 0.07423066716382465 1104 2 0.04412760021929785 1104 3 -0.05831617712866688 1104 4 0.06265405128446133 1104 5 0.16225088589128944 1104 6 0.19582455359645934 1104 7 0.11189551072973813 1104 8 0.2132516541408762 1104 9 0.1835454260379663 1104 10-11 0.248920639460529 1104 12-13 0.1674207081231316 1104 14-15 0.20298617597411095 1104 16-17 0.12078726350497959 1104 18-19 0.19377424502442153 1104 20-21 0.2238270038200696 1104 22-23 0.28133813832865684 1104 24-25 0.2718551077598903 1104 26-27 0.3579262114074382 1104 28-29 0.3301857452707182 1104 30-31 0.26310596375026307 1104 32-33 0.10002227033674416 1104 34-35 0.216271393346382 1104 36-37 0.1571076406083165 1104 38-39 0.3274383313369498 1104 40-41 0.4728344197540082 1104 42-43 0.5456986575896323 1104 44-45 0.5370797897041228 1104 46-47 0.31970083005657557 1104 48-49 0.19943814117058167 1104 50-51 0.3170225703882821 1104 52-53 0.24201236490656441 1104 54-55 0.2769853267175435 1104 56-57 0.24170429252976078 1104 58-59 0.43417141904853906 1104 60-61 0.26656387988953867 1104 62-63 0.29323163976897604 1104 64-65 0.25139365043688855 1104 66-67 0.16666428659024035 1104 68-69 0.384861765887095 1104 70-71 0.36341851194633534 1104 72-73 0.13026794715253232 1104 74-75 0.2757879621938386 1104 76-77 0.29509018981004687 1104 78-79 0.3629995954544327 1104 80-81 0.3793972815087372 1104 82-83 0.32782965991154356 1104 84-85 0.3726092064274873 1104 86-87 0.3152584765987889 1104 88-89 0.3969709241832682 1104 90-91 0.6045706538778717 1104 92-93 0.543758237829465 1104 94-95 0.33279744098336295 1104 96-97 0.35698264557361625 1104 98-99 0.40426938228858234 1104 100-101 0.48508358761748127 1105 1 0.03167467243391542 1105 2 0.11737540254764411 1105 3 0.040930026893022387 1105 4 0.040911681038885206 1105 5 0.03934542357028903 1105 6 0.09270893554855064 1105 7 0.09367373488144892 1105 8 0.10227517516725015 1105 9 0.10225533337938231 1105 10-11 0.0703309290996117 1105 12-13 0.1121511558649857 1105 14-15 0.04976904385462433 1105 16-17 0.11737412283239479 1105 18-19 0.10923462213840907 1105 20-21 0.18998502019191932 1105 22-23 0.052501122410383516 1105 24-25 0.13456650541426285 1105 26-27 0.19673482498440364 1105 28-29 0.0643679090323559 1105 30-31 0.11307194573691959 1105 32-33 0.12119821366165695 1105 34-35 0.13947815807132002 1105 36-37 0.11250192558208028 1105 38-39 0.21162265497245158 1105 40-41 0.19215341152488463 1105 42-43 0.14110676575612047 1105 44-45 -0.019800488487632606 1105 46-47 -0.013670841037829007 1105 48-49 0.15902636121852964 1105 50-51 0.10999326089996231 1105 52-53 0.23965270271083483 1105 54-55 0.22282000997203966 1105 56-57 0.15400801399242425 1105 58-59 0.23498461155809025 1105 60-61 0.21663461575857212 1105 62-63 0.0730163003447899 1105 64-65 0.037630934179517794 1105 66-67 0.14692951910066654 1105 68-69 -0.08580066742680259 1105 70-71 -0.23602910031311097 1105 72-73 -0.18223124289476544 1105 74-75 -0.0195175519641424 1105 76-77 -0.030302367264624763 1105 78-79 -0.12795377286885667 1105 80-81 -0.045157244506945915 1105 82-83 0.06366736698944919 1105 84-85 0.028042909099252 1105 86-87 0.11918458731392079 1105 88-89 0.0025056009581412297 1105 90-91 0.33112360318563105 1105 92-93 0.290923943352265 1105 94-95 0.14132354305900208 1105 96-97 0.08581264190183191 1105 98-99 0.024498760892868177 1105 100-101 0.22339974995360024 1106 1 -0.1043897074364537 1106 2 -0.12201929349988205 1106 3 -0.061377577380937964 1106 4 -0.06518869662583882 1106 5 0.024001623826229945 1106 6 -0.09792383492909806 1106 7 0.0032989832766290306 1106 8 -0.05329772036632363 1106 9 -0.11790915365205024 1106 10-11 0.06110857509355583 1106 12-13 -0.0652911800381375 1106 14-15 0.036544536223303226 1106 16-17 0.006650473529980161 1106 18-19 -0.04249920226310877 1106 20-21 0.05093625670574653 1106 22-23 0.03897880065668602 1106 24-25 0.06998349720552 1106 26-27 -0.11528854236996011 1106 28-29 0.05883350824998246 1106 30-31 0.04782100157511593 1106 32-33 0.07721770032894426 1106 34-35 0.10965356928890202 1106 36-37 0.10465835992771133 1106 38-39 0.05325524858231745 1106 40-41 0.07552211841336032 1106 42-43 0.10122672372401098 1106 44-45 0.19418611117736617 1106 46-47 0.29618667636283647 1106 48-49 0.245312606107575 1106 50-51 0.33905523744936517 1106 52-53 0.24838684963594204 1106 54-55 0.005474483166160837 1106 56-57 0.16945273240852288 1106 58-59 0.22747501388026592 1106 60-61 0.21620831239911809 1106 62-63 0.2454597806864598 1106 64-65 0.3272311089664939 1106 66-67 0.2135446860326411 1106 68-69 0.2258387928143648 1106 70-71 0.15522296747926845 1106 72-73 0.06172612156729684 1106 74-75 0.43845086153191914 1106 76-77 0.1324607235517803 1106 78-79 0.004745957667886103 1106 80-81 0.12216851472398815 1106 82-83 0.12317400993502758 1106 84-85 0.13838213108912 1106 86-87 0.24450659070562253 1106 88-89 0.22315127237943244 1106 90-91 0.3940310493189898 1106 92-93 0.28491746166848486 1106 94-95 0.1478041864415438 1106 96-97 0.1379751105598217 1106 98-99 0.03557900684311832 1106 100-101 0.45001522900517443 1107 1 -0.12133674510787529 1107 2 0.0038902715239927943 1107 3 -0.10479902731576374 1107 4 -0.12464431071693127 1107 5 -0.05673204559580114 1107 6 -0.008169462724097798 1107 7 -0.0242001160034917 1107 8 -0.02666125954993248 1107 9 0.004272214329425594 1107 10-11 0.008641034769127032 1107 12-13 0.01835801293307071 1107 14-15 -0.027327171017624607 1107 16-17 0.05591323363816514 1107 18-19 -0.008284270785331671 1107 20-21 -0.07592102227217623 1107 22-23 -0.08072997648711322 1107 24-25 0.04781363467130717 1107 26-27 0.08309102611634245 1107 28-29 -0.021458308784239932 1107 30-31 -0.1470934286864818 1107 32-33 -0.0098426146826327 1107 34-35 0.09252719788550223 1107 36-37 -0.02385100404791274 1107 38-39 0.027672643522194562 1107 40-41 -0.037236126211894316 1107 42-43 -0.1715228675085143 1107 44-45 -0.1573098940557216 1107 46-47 -0.18390916995463158 1107 48-49 -0.35825289418762196 1107 50-51 -0.21036038846046523 1107 52-53 -0.1279168984930763 1107 54-55 0.06364181126101442 1107 56-57 -0.1588784321750154 1107 58-59 -0.2320811783460499 1107 60-61 -0.10571383191375361 1107 62-63 -0.12087744127198619 1107 64-65 -0.32131825939524816 1107 66-67 -0.1576685768852002 1107 68-69 -0.0033964406462843044 1107 70-71 0.05587802174662926 1107 72-73 -0.14645900486029007 1107 74-75 -0.23257248048182078 1107 76-77 -0.34298270880942283 1107 78-79 -0.3111676963811334 1107 80-81 -0.2233608627880983 1107 82-83 0.06789087600243704 1107 84-85 -0.2152186147663535 1107 86-87 -0.18701736250124057 1107 88-89 -0.11148006986332959 1107 90-91 -0.5644099787818462 1107 92-93 -0.26086638608798296 1107 94-95 -0.1476678528908799 1107 96-97 -0.6389820713510233 1107 98-99 -0.6724783713085216 1107 100-101 -0.24649916063624744 1108 1 -0.15924616241328238 1108 2 -0.14433990971266297 1108 3 -0.13106545169080874 1108 4 -0.03294080775850006 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72-73 -0.21825394127444042 2316 74-75 -0.17573764765791822 2316 76-77 -0.08865014712269925 2316 78-79 -0.2421437273334064 2316 80-81 -0.3504377382313706 2316 82-83 -0.3671071978812215 2316 84-85 -0.20385212823056875 2316 86-87 -0.27302295723916004 2316 88-89 -0.1647920899093407 2316 90-91 -0.16173345711994003 2316 92-93 -0.16470037766107026 2316 94-95 -0.1812014783218565 2316 96-97 -0.08726134741930025 2316 98-99 -0.287453715819332 2316 100-101 -0.2135463702877125 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 15.0 5 24.0 6 44.0 7 92.0 8 281.0 9 461.0 10 728.0 11 1280.0 12 1815.0 13 2713.0 14 3431.0 15 4462.0 16 5820.0 17 7738.0 18 7497.0 19 5343.0 20 5008.0 21 5422.0 22 5976.0 23 6643.0 24 7766.0 25 9439.0 26 11492.0 27 14303.0 28 18193.0 29 22382.0 30 27796.0 31 35160.0 32 42963.0 33 55858.0 34 76852.0 35 114201.0 36 195311.0 37 355095.0 38 373308.0 39 63707.0 40 69.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.45501850123185 17.935553481154606 13.612043918031528 23.99738409958202 2 15.994541841181285 18.883321441480664 40.1659081411489 24.95622857618915 3 20.19511087585612 22.83501221206267 28.185413772627243 28.784463139453965 4 13.273665741469692 15.317742017825045 35.31784949472423 36.09074274598104 5 14.547065477613904 36.53489103185884 33.54898125334186 15.369062237185396 6 34.93382109932746 34.258060095708174 16.418574157716975 14.389544647247382 7 30.772315562923698 30.86212594680431 19.37835361511985 18.98720487515215 8 27.12260158582165 35.83024561158386 18.246420347526556 18.80073245506794 9 26.554922038944255 16.419178715274885 19.058744186171484 37.967155059609375 10-11 26.330076204915283 25.7111064910708 26.21339593002811 21.745421373985806 12-13 26.47628906075507 22.551766132718654 27.276018756081267 23.695926050445003 14-15 23.087650098207018 24.29223103234249 24.972324698460124 27.647794170990377 16-17 23.45889945115973 27.57243055865766 25.473530489502643 23.49513950067996 18-19 23.335585870012064 26.264532891961533 27.32254220483485 23.077339033191553 20-21 23.93077278577436 25.631326023112905 27.42639175867137 23.011509432441365 22-23 23.58232044718466 25.399251624665016 27.40937259331433 23.609055334835997 24-25 23.32126981034798 25.647475308963337 27.57818758302419 23.45306729766449 26-27 23.413238213274486 25.8643598708893 27.41452797678426 23.307873939051955 28-29 23.205422868979017 25.9824533296253 27.466910644718045 23.345213156677637 30-31 23.412708542132968 25.724204456601473 27.435566560173257 23.427520441092305 32-33 23.305026110625217 25.731090788665828 27.482692558303878 23.481190542405077 34-35 23.629312142904404 25.90619278619456 27.265670893847787 23.198824177053247 36-37 23.647772675610536 25.840670870804704 27.18366189917055 23.32789455441421 38-39 23.370766666823403 25.900203601687654 27.33596159310428 23.393068138384663 40-41 23.509459222082388 25.756383425713864 27.393068823746148 23.341088528457604 42-43 23.532442581433934 25.69584114252702 27.19362769795046 23.578088578088575 44-45 23.504546659138445 25.953962074282867 27.11302175403335 23.428469512545337 46-47 23.476949629770466 26.197665587145448 26.852063043322715 23.47332173976137 48-49 23.7123559211523 26.070188439159953 26.823347147345466 23.394108492342276 50-51 23.61699197560099 26.150270223064187 26.725749621284645 23.506988180050183 52-53 23.749328209813513 26.251545117428925 26.53245418390928 23.466672488848282 54-55 23.590790285949584 26.11146451676609 26.597630532636433 23.700114664647888 56-57 23.533944138161285 26.458229910985686 26.652568759207245 23.35525719164578 58-59 23.727504233647643 26.152522824079767 26.734552811941402 23.385420130331187 60-61 23.653519907219103 26.123445212953722 26.84093201407835 23.38210286574882 62-63 23.49663328613306 26.253449336733187 26.780892219478574 23.469025157655178 64-65 23.4843283983126 26.121328962570868 26.758369830991214 23.63597280812532 66-67 23.44413753818789 26.321629994653023 26.642146259938254 23.592086207220834 68-69 23.319665854320437 26.21411950893805 26.86482496043507 23.60138967630645 70-71 23.422337760044066 26.02872323989548 26.797755073252322 23.751183926808135 72-73 23.51167785000467 26.212606798134985 26.573465092736953 23.702250259123396 74-75 23.40017276911544 26.41392578182727 26.429912970580887 23.755988478476407 76-77 23.517673638849498 26.355631800368407 26.364044453806795 23.7626501069753 78-79 23.52861869997586 26.40713119058714 26.392236999859463 23.672013109577534 80-81 23.44185474753758 26.563015348527586 26.411740088682773 23.583389815252065 82-83 23.60266596448426 26.51535710543215 26.43404411389327 23.44793281619032 84-85 23.64753279336861 26.354264103541563 26.2020484624776 23.796154640612222 86-87 23.46262400851199 26.328863797702983 26.567389237670273 23.64112295611475 88-89 23.641827893527942 26.363121325458277 26.35785116735691 23.637199613656872 90-91 23.557243966730095 26.56575954245794 26.377396732304476 23.49959975850749 92-93 23.49926145005061 26.35708763733071 26.439481852892975 23.7041690597257 94-95 23.49918315581303 26.402070055136985 26.42715980051805 23.67158698853194 96-97 23.55144920575316 26.34688101848462 26.368645806953044 23.733023968809178 98-99 23.34806496967352 26.374835167566484 26.28240137092218 23.994698491837813 100-101 23.39539054733052 26.447456807372976 26.23968885186877 23.917463793427736 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 72.0 1 55.5 2 79.5 3 140.5 4 117.0 5 105.0 6 214.0 7 480.5 8 808.5 9 934.5 10 861.0 11 782.0 12 823.0 13 919.0 14 978.5 15 1048.0 16 1121.5 17 1198.0 18 1310.5 19 1443.0 20 1589.0 21 1839.5 22 2167.5 23 2656.5 24 3330.0 25 4180.0 26 5365.0 27 6722.5 28 8120.0 29 9840.5 30 11868.5 31 14168.0 32 16657.5 33 18810.0 34 21411.0 35 24042.5 36 27049.5 37 29980.5 38 32312.0 39 34615.0 40 36839.0 41 40455.5 42 43726.5 43 46221.5 44 49331.5 45 52359.5 46 54689.5 47 56331.5 48 58416.0 49 59114.5 50 58270.0 51 58404.0 52 59239.0 53 59912.0 54 59730.0 55 56912.0 56 52818.5 57 48623.0 58 44872.0 59 40719.0 60 35232.0 61 29311.5 62 23530.5 63 18815.5 64 14544.0 65 10804.5 66 7695.5 67 5930.5 68 4390.0 69 2950.5 70 2208.0 71 1643.0 72 1316.5 73 1076.0 74 819.5 75 575.5 76 338.0 77 162.0 78 83.0 79 42.5 80 23.5 81 14.0 82 8.5 83 3.0 84 2.5 85 2.0 86 2.0 87 1.0 88 0.0 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26372144137269493 2 0.01867411123321681 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.709710269149025E-4 12-13 1.007595929849828E-4 14-15 0.0 16-17 5.03797964924914E-4 18-19 0.0 20-21 0.0 22-23 3.358653099499426E-5 24-25 0.0029892012585544894 26-27 0.0031571339135294607 28-29 0.0022502975766646154 30-31 0.0016121534877597248 32-33 0.0017464996117397017 34-35 0.002183124514674627 36-37 0.0 38-39 6.717306198998852E-5 40-41 8.396632748748566E-4 42-43 0.004164729843379289 44-45 0.00480287393228418 46-47 0.015013179354762436 48-49 0.01528187160272239 50-51 0.007187517632928772 52-53 0.008866844182678486 54-55 2.015191859699656E-4 56-57 0.003694518409449369 58-59 0.001544980425769736 60-61 0.0015785669567647304 62-63 0.0 64-65 8.060767438798624E-4 66-67 0.0 68-69 0.0 70-71 1.3434612397997703E-4 72-73 0.001276288177809782 74-75 0.0 76-77 0.19043563074161748 78-79 0.10311065015463239 80-81 0.001007595929849828 82-83 0.0 84-85 9.740093988548337E-4 86-87 0.12416940508849379 88-89 0.5817523033642956 90-91 0.3081900084100674 92-93 0.29317682905530495 94-95 0.002418230231639587 96-97 0.0032914800375094377 98-99 0.003963210657409323 100-101 8.060767438798624E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1488692.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.19488044279886 #Duplication Level Percentage of deduplicated Percentage of total 1 78.51260096118773 48.045692290730585 2 12.756592326126102 15.612762845096242 3 3.8049442097053308 6.985293180133125 4 1.6633929825369955 4.071645387829682 5 0.880036379343189 2.6926860509610018 6 0.5189123861477977 1.9052868858361172 7 0.35547089711503044 1.5227099334894123 8 0.24424192151537805 1.195708414900242 9 0.18990462328229127 1.0459071645565114 >10 0.9824386354120671 10.713682962741355 >50 0.06436595830949306 2.6800209177512566 >100 0.025562840226908874 2.5493839268521263 >500 0.0012067621434641148 0.4959877724554928 >1k 3.291169482174859E-4 0.48323226666684316 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3236 0.21737202859960286 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2354 0.15812538792443298 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1608 0.10801428367990155 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 3.3586530994994265E-5 14-15 0.0 0.0 0.0 0.0 1.007595929849828E-4 16-17 0.0 0.0 0.0 3.3586530994994265E-5 1.3434612397997706E-4 18-19 0.0 0.0 0.0 6.717306198998853E-5 1.3434612397997706E-4 20-21 0.0 0.0 0.0 1.3434612397997706E-4 2.0151918596996558E-4 22-23 0.0 0.0 0.0 5.709710269149025E-4 2.0151918596996558E-4 24-25 0.0 0.0 0.0 8.060767438798623E-4 2.686922479599541E-4 26-27 0.0 0.0 0.0 9.740093988548336E-4 3.3586530994994263E-4 28-29 0.0 0.0 0.0 0.001141942053829805 3.3586530994994263E-4 30-31 0.0 0.0 0.0 0.0023510571696495983 3.3586530994994263E-4 32-33 0.0 0.0 0.0 0.004063970250394306 4.0303837193993115E-4 34-35 0.0 0.0 0.0 0.006112748641088956 4.0303837193993115E-4 36-37 0.0 0.0 0.0 0.010075959298498279 4.0303837193993115E-4 38-39 0.0 0.0 0.0 0.017297063462422047 4.0303837193993115E-4 40-41 0.0 0.0 0.0 0.026969984388980392 4.0303837193993115E-4 42-43 0.0 0.0 0.0 0.03603834775762885 4.0303837193993115E-4 44-45 0.0 0.0 0.0 0.046752451145032015 4.0303837193993115E-4 46-47 0.0 0.0 0.0 0.05954891945412483 4.3662490293492544E-4 48-49 0.0 0.0 0.0 0.07617425229664698 4.7021143392991967E-4 50-51 0.0 0.0 0.0 0.09273241207717917 5.373844959199082E-4 52-53 0.0 0.0 0.0 0.11170880208935093 6.045575579098967E-4 54-55 0.0 0.0 0.0 0.13108823047346263 6.38144088904891E-4 56-57 0.0 0.0 0.0 0.1526507833722489 7.053171508948795E-4 58-59 0.0 0.0 0.0 0.17995663307117926 7.389036818898738E-4 60-61 0.0 0.0 0.0 0.20998299178070412 7.389036818898738E-4 62-63 0.0 0.0 0.0 0.2412520521370438 7.389036818898738E-4 64-65 0.0 0.0 0.0 0.27366305454721324 7.389036818898738E-4 66-67 0.0 0.0 0.0 0.3118845268195167 7.389036818898738E-4 68-69 0.0 0.0 0.0 0.35873773755753374 7.389036818898738E-4 70-71 0.0 0.0 0.0 0.4122074949015646 7.389036818898738E-4 72-73 0.0 0.0 0.0 0.4657780118385805 7.389036818898738E-4 74-75 0.0 0.0 0.0 0.5195500479615662 7.389036818898738E-4 76-77 0.0 0.0 0.0 0.5838682548169802 7.389036818898738E-4 78-79 0.0 0.0 0.0 0.6611508626364622 7.389036818898738E-4 80-81 0.0 0.0 0.0 0.7458225072748426 7.724902128848681E-4 82-83 0.0 0.0 0.0 0.8337856319507326 8.732498058698509E-4 84-85 0.0 0.0 0.0 0.9264844574969168 8.732498058698509E-4 86-87 0.0 0.0 0.0 1.0205939173448906 8.732498058698509E-4 88-89 0.0 0.0 0.0 1.1283059222458374 8.732498058698509E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1910 0.0 45.07697 1 GTATCAA 3195 0.0 42.877666 1 ATCAACG 4125 0.0 33.119236 3 TCAACGC 4170 0.0 32.875584 4 TATCAAC 4230 0.0 32.41689 2 CAACGCA 4260 0.0 32.181034 5 AACGCAG 4435 0.0 31.232086 6 ATACCGT 100 4.9995277E-5 28.461842 6 GTACCTG 1350 0.0 25.721638 1 ACGCAGA 5460 0.0 25.0214 7 CGCAGAG 5630 0.0 24.097355 8 GTACATG 4925 0.0 23.469809 1 TACCTGG 1530 0.0 22.948492 2 TACATGG 4890 0.0 22.801996 2 GTGGTAT 1060 0.0 22.437458 1 ACATGGG 5035 0.0 21.9517 3 GTGTAGC 945 0.0 20.637712 1 TATCACG 115 0.003747695 20.629372 2 GCAGAGT 6510 0.0 20.402754 9 CTACACG 280 1.6025297E-9 20.329887 4 >>END_MODULE