##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936314_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 56353 Sequences flagged as poor quality 0 Sequence length 101 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.080031231700175 33.0 31.0 34.0 30.0 34.0 2 32.12442993274537 33.0 31.0 34.0 30.0 34.0 3 32.142139726367716 34.0 31.0 34.0 30.0 34.0 4 35.73296896349795 37.0 35.0 37.0 33.0 37.0 5 35.777456390964105 37.0 35.0 37.0 35.0 37.0 6 35.94500736429294 37.0 35.0 37.0 35.0 37.0 7 35.88183415257395 37.0 35.0 37.0 35.0 37.0 8 35.92113995705641 37.0 35.0 37.0 35.0 37.0 9 37.50105584440935 39.0 37.0 39.0 35.0 39.0 10-11 37.52032722304048 39.0 37.0 39.0 35.0 39.0 12-13 37.46109346441183 39.0 37.0 39.0 34.5 39.0 14-15 38.33394850318528 40.0 38.0 41.0 33.0 41.0 16-17 38.214256561318834 39.5 37.0 41.0 33.5 41.0 18-19 38.14312458964031 40.0 36.5 41.0 34.0 41.0 20-21 38.13274359838873 40.0 36.0 41.0 34.0 41.0 22-23 37.90672191365145 40.0 35.0 41.0 34.0 41.0 24-25 37.781866094085494 40.0 35.0 41.0 33.5 41.0 26-27 37.350291909924934 39.0 35.0 41.0 33.0 41.0 28-29 36.950215605202914 39.0 35.0 41.0 33.0 41.0 30-31 36.453134704452296 37.5 35.0 40.5 33.0 41.0 32-33 35.789585292708466 36.0 35.0 40.0 31.5 41.0 34-35 35.44940819477224 35.0 35.0 40.0 31.0 41.0 36-37 35.11710113037461 35.0 35.0 40.0 30.5 41.0 38-39 34.77852998065764 35.0 35.0 40.0 30.0 41.0 40-41 34.441209873476126 35.0 35.0 40.0 28.0 41.0 42-43 34.037247351516335 35.0 35.0 40.0 24.0 41.0 44-45 33.44593011907086 35.0 34.5 40.0 20.5 41.0 46-47 32.759409436942136 35.0 33.5 40.0 5.0 41.0 48-49 32.18400085177364 35.0 33.0 40.0 2.0 41.0 50-51 31.683645946089825 35.0 33.0 40.0 2.0 41.0 52-53 31.368436463009957 35.0 33.0 40.0 2.0 41.0 54-55 31.068053164871436 35.0 33.0 40.0 2.0 41.0 56-57 30.598042695153765 35.0 31.5 40.0 2.0 41.0 58-59 30.21094706581726 35.0 31.0 39.0 2.0 41.0 60-61 29.694994055329797 35.0 30.0 39.0 2.0 41.0 62-63 29.213653221656344 35.0 28.0 39.0 2.0 41.0 64-65 28.7686547300055 35.0 28.0 38.0 2.0 40.0 66-67 28.22244600997285 35.0 26.0 37.0 2.0 40.0 68-69 27.68957287100953 35.0 25.5 36.5 2.0 39.0 70-71 27.12068567778113 35.0 23.5 36.0 2.0 38.5 72-73 26.60816637978457 34.5 20.5 35.0 2.0 37.0 74-75 26.18157862048161 34.0 17.5 35.0 2.0 37.0 76-77 25.498651358401506 33.0 10.0 35.0 2.0 36.0 78-79 25.441183255549838 34.0 4.0 35.0 2.0 36.0 80-81 25.26241726261246 34.0 2.0 35.0 2.0 35.5 82-83 24.995900839351943 33.5 2.0 35.0 2.0 35.0 84-85 24.677754511738506 33.0 2.0 35.0 2.0 35.0 86-87 24.328731389633205 33.0 2.0 35.0 2.0 35.0 88-89 23.79246002874736 33.0 2.0 35.0 2.0 35.0 90-91 23.459762568097528 33.0 2.0 35.0 2.0 35.0 92-93 23.069179990417545 33.0 2.0 35.0 2.0 35.0 94-95 22.86942132628254 33.0 2.0 35.0 2.0 35.0 96-97 22.57562152857878 32.0 2.0 35.0 2.0 35.0 98-99 22.214966372686458 32.0 2.0 35.0 2.0 35.0 100-101 20.295751778964743 28.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.007688973403503496 1101 2 -0.2843581417459582 1101 3 -0.5447718088512836 1101 4 0.1603161718495798 1101 5 -0.023194235940202645 1101 6 -0.13760903738459973 1101 7 0.31436010388573266 1101 8 0.14265316903326664 1101 9 0.4247191947001099 1101 10-11 0.07212572121760275 1101 12-13 -0.20266037249762547 1101 14-15 -0.17472998044618038 1101 16-17 0.22827367872266535 1101 18-19 0.47986957238710204 1101 20-21 0.28865400944066977 1101 22-23 0.48399406056230276 1101 24-25 1.1939223583429168 1101 26-27 0.9051168714412867 1101 28-29 1.448459380710645 1101 30-31 1.3055073243167357 1101 32-33 1.5600362647435304 1101 34-35 1.8735555979815572 1101 36-37 1.7561625029776877 1101 38-39 1.6752728603746974 1101 40-41 2.1287813850104484 1101 42-43 1.415236906343175 1101 44-45 1.0760084210262946 1101 46-47 1.8290124479678482 1101 48-49 2.033737236460148 1101 50-51 1.7660380740077173 1101 52-53 2.387669289552722 1101 54-55 2.6724178080098575 1101 56-57 2.400964050433796 1101 58-59 2.411633823636315 1101 60-61 2.3063898653556762 1101 62-63 2.3057818891679958 1101 64-65 2.802391328242752 1101 66-67 3.46586196496537 1101 68-69 3.0686577358545897 1101 70-71 2.6314107681274983 1101 72-73 2.3263253870987803 1101 74-75 2.318033300215177 1101 76-77 2.6012105681389137 1101 78-79 2.849863128143138 1101 80-81 2.096831195894495 1101 82-83 2.7961935949890773 1101 84-85 2.982886381961375 1101 86-87 2.8411341455797974 1101 88-89 2.7925905272430676 1101 90-91 3.6402067967796903 1101 92-93 3.4093269107646655 1101 94-95 3.462389988069919 1101 96-97 3.2182754551404713 1101 98-99 3.3514779497657052 1101 100-101 3.319788811316158 1102 1 -0.9832324516643745 1102 2 -0.37856104029668103 1102 3 -1.1498442726193971 1102 4 -0.5597925238025923 1102 5 -0.18623771420107005 1102 6 -0.537971356225178 1102 7 -0.6765819250997751 1102 8 -0.4850642222710846 1102 9 -0.47020834153177304 1102 10-11 -0.6185445686374678 1102 12-13 -0.18137414061356338 1102 14-15 -0.8101466471128518 1102 16-17 -1.0226321183787874 1102 18-19 -1.2669601377578275 1102 20-21 -1.5555488891100495 1102 22-23 -1.5984334756695873 1102 24-25 -1.4614218445556375 1102 26-27 -1.225770809718135 1102 28-29 -2.0008159816081985 1102 30-31 -2.0862499220600768 1102 32-33 -1.546847793227478 1102 34-35 -1.4525313585401776 1102 36-37 -2.216210685428109 1102 38-39 -1.9859590236832787 1102 40-41 -2.537885281656223 1102 42-43 -2.8324986009032003 1102 44-45 -1.0064191152055955 1102 46-47 -2.175063638988675 1102 48-49 -2.7167156620905786 1102 50-51 -2.5826938100502517 1102 52-53 -2.423472014795106 1102 54-55 -1.8112778441640565 1102 56-57 -1.8685105872473606 1102 58-59 -2.820250234334697 1102 60-61 -2.922323902760265 1102 62-63 -3.4559934731508406 1102 64-65 -3.1948912804529 1102 66-67 -4.482960498802743 1102 68-69 -5.40009226414541 1102 70-71 -5.196034884046416 1102 72-73 -4.79867461290122 1102 74-75 -5.444194960654386 1102 76-77 -4.838553924614708 1102 78-79 -4.149231074755416 1102 80-81 -4.5064296736707234 1102 82-83 -4.582429593416723 1102 84-85 -4.965030284705293 1102 86-87 -4.855423825434695 1102 88-89 -5.3704529510178 1102 90-91 -5.230717116263786 1102 92-93 -4.759151350104901 1102 94-95 -4.712881751060518 1102 96-97 -4.52221367529431 1102 98-99 -4.321076398060381 1102 100-101 -4.569703942307028 1103 1 -1.2658411473165465 1103 2 -0.9655175620358136 1103 3 -1.4324529682715728 1103 4 -0.7771838281504202 1103 5 -0.36015075767933524 1103 6 -0.32058005187735006 1103 7 -0.24179931640412633 1103 8 -0.6372381353145613 1103 9 -1.0136866024013358 1103 10-11 -1.0098489164635538 1103 12-13 -0.8226784884396494 1103 14-15 -1.4514509949389378 1103 16-17 -0.7835016835961781 1103 18-19 -0.4843514421056483 1103 20-21 -0.9468532369361427 1103 22-23 -1.1201726061043686 1103 24-25 -0.9940305402078096 1103 26-27 -1.8562055923268304 1103 28-29 -1.2508159816081985 1103 30-31 -0.8145107916252883 1103 32-33 -1.7207608367057432 1103 34-35 -2.5612270107140986 1103 36-37 -2.857515033254195 1103 38-39 -3.4968285889006694 1103 40-41 -3.1031026729605706 1103 42-43 -3.2455420791640677 1103 44-45 -3.9303321586838535 1103 46-47 -4.00115059551041 1103 48-49 -4.662367836003625 1103 50-51 -3.65878076657199 1103 52-53 -3.98868940609945 1103 54-55 -4.583016974598838 1103 56-57 -4.0641627611604 1103 58-59 -2.907206756073826 1103 60-61 -2.6179760766733082 1103 62-63 -3.2820804296725825 1103 64-65 -3.760108671757248 1103 66-67 -4.080786585759267 1103 68-69 -3.0848748728410627 1103 70-71 -3.522121840568154 1103 72-73 -3.5486746129012197 1103 74-75 -3.5311514823935184 1103 76-77 -3.371162620266883 1103 78-79 -3.4753180312771548 1103 80-81 -3.832516630192462 1103 82-83 -3.8541687238515046 1103 84-85 -2.9650302847052927 1103 86-87 -2.931510781956433 1103 88-89 -3.587844255365628 1103 90-91 -2.872021464089876 1103 92-93 -3.2156730892353345 1103 94-95 -2.865055664103995 1103 96-97 -3.4678658492073566 1103 98-99 -4.571076398060381 1103 100-101 -4.102312637959201 1104 1 0.20640677127735074 1104 2 0.2440106525802861 1104 3 -0.04299875920589358 1104 4 -0.09633276432063553 1104 5 0.1629759768257557 1104 6 -0.18459485298743772 1104 7 0.27623953650984134 1104 8 0.48440848818220417 1104 9 0.48677593228876503 1104 10-11 0.03201047299065607 1104 12-13 -0.133733067533079 1104 14-15 0.4877256933126759 1104 16-17 -0.027488732624853185 1104 18-19 -0.01857900917318034 1104 20-21 0.1893632292988272 1104 22-23 -0.2637896273809659 1104 24-25 0.08598796117979646 1104 26-27 0.20808672959731211 1104 28-29 0.8615799480865292 1104 30-31 0.4382644165153238 1104 32-33 -0.36992827426356456 1104 34-35 -0.7275082318056789 1104 36-37 -0.533509482837907 1104 38-39 -0.20681933820686282 1104 40-41 0.4833913140884576 1104 42-43 0.17166421130772136 1104 44-45 -1.5494348413850574 1104 46-47 -0.6995779775640685 1104 48-49 -0.8973354585753164 1104 50-51 -0.7556817841483081 1104 52-53 -2.413527518957917 1104 54-55 -0.7025821919901425 1104 56-57 -0.5766511977931508 1104 58-59 -0.11673497068992589 1104 60-61 0.5402107873414899 1104 62-63 0.42235990335239393 1104 64-65 -0.01542782069341797 1104 66-67 -0.15802987900626064 1104 68-69 0.5839502890460793 1104 70-71 0.902022470255158 1104 72-73 1.110899855183888 1104 74-75 0.6684322363853887 1104 76-77 1.48707049721693 1104 78-79 1.5364766033204411 1104 80-81 1.4026822597242798 1104 82-83 1.685377992152194 1104 84-85 0.9870973748691725 1104 86-87 1.165158968274831 1104 88-89 0.9202501017111508 1104 90-91 1.3150738180562875 1104 92-93 1.6673056341689225 1104 94-95 0.3021506263677942 1104 96-97 0.026121199821321994 1104 98-99 -0.38721885874493367 1104 100-101 0.9962072510324731 1105 1 0.3583824551679271 1105 2 0.47414525961458764 1105 3 0.38209893944803497 1105 4 0.3452651514414171 1105 5 -0.120133011450406 1105 6 0.295214091867102 1105 7 0.003985954225868227 1105 8 0.2842348016863312 1105 9 0.6305015076252829 1105 10-11 0.4881546327822335 1105 12-13 -0.07489676705544213 1105 14-15 0.34291457737694486 1105 16-17 0.3511478283825298 1105 18-19 0.40695290572043774 1105 20-21 0.9655247577399919 1105 22-23 0.8543171898139974 1105 24-25 1.3484716780708013 1105 26-27 1.0839008850467238 1105 28-29 0.24607842832968885 1105 30-31 0.5455158277181056 1105 32-33 0.9928593939952393 1105 34-35 1.6235555979815572 1105 36-37 1.6870723669232675 1105 38-39 1.5519735406468058 1105 40-41 1.3498698203845905 1105 42-43 1.3631535730098463 1105 44-45 1.482045835992274 1105 46-47 1.3258236724576378 1105 48-49 1.4268069643513002 1105 50-51 1.4297285501981918 1105 52-53 1.7582050038384303 1105 54-55 1.6545606651527116 1105 56-57 1.5121460232229147 1105 58-59 1.1429263406431218 1105 60-61 1.6025208177366288 1105 62-63 1.4184519571952094 1105 64-65 2.076626022120305 1105 66-67 1.5540847540810176 1105 68-69 1.605009776670915 1105 70-71 0.7085791354744373 1105 72-73 1.237039672813065 1105 74-75 1.9615281981743635 1105 76-77 1.322936758615107 1105 78-79 1.8239277539934768 1105 80-81 1.8340760938536782 1105 82-83 1.4535065201591415 1105 84-85 1.7135411438661379 1105 86-87 1.8874776829947635 1105 88-89 1.8232027721410269 1105 90-91 2.350028225351121 1105 92-93 2.498612625050381 1105 94-95 2.1058848860291057 1105 96-97 1.4229948428955765 1105 98-99 1.5906361130310138 1105 100-101 1.8342445936290872 1106 1 0.5779591747446418 1106 2 0.2341603767725644 1106 3 0.038561524482048526 1106 4 0.27837172740513694 1106 5 0.5786576159116521 1106 6 0.5522057774302169 1106 7 0.3838045483301755 1106 8 -0.36429127541117623 1106 9 -0.08454006455914964 1106 10-11 0.5408757212176027 1106 12-13 0.17812666453941262 1106 14-15 0.7454089084427054 1106 16-17 0.7583662713152535 1106 18-19 1.0921380909056282 1106 20-21 1.0386540094406698 1106 22-23 1.2872348013030361 1106 24-25 1.3316538398243907 1106 26-27 1.3136816862560963 1106 28-29 1.3929038251550878 1106 30-31 0.797405472464888 1106 32-33 0.44429552400278993 1106 34-35 1.1791111535371144 1106 36-37 0.25037546594065674 1106 38-39 0.6799024900043165 1106 40-41 0.5732258294548913 1106 42-43 1.4927832026394796 1106 44-45 0.08295286547073744 1106 46-47 0.19128096648636728 1106 48-49 -0.9002905413176308 1106 50-51 -1.3057211852515387 1106 52-53 -1.6042288585954267 1106 54-55 -2.535915525323478 1106 56-57 -2.475193356973609 1106 58-59 -1.6068846948822006 1106 60-61 -1.5443045790887666 1106 62-63 -1.664125518239409 1106 64-65 -1.2045531162016907 1106 66-67 -1.174184331330924 1106 68-69 -1.0482404122935591 1106 70-71 -1.0688207133539827 1106 72-73 -0.8542301684567768 1106 74-75 -1.169235218303342 1106 76-77 -0.5249468392684911 1106 78-79 -0.6987479829679764 1106 80-81 -0.7781688041055048 1106 82-83 -0.8658434420479608 1106 84-85 -0.15021546989047607 1106 86-87 -0.2584028914572407 1106 88-89 -0.05926132460878364 1106 90-91 0.2918271671500605 1106 92-93 0.3491417255794822 1106 94-95 1.0954918399217703 1106 96-97 1.2495254551404713 1106 98-99 1.3109686905064457 1106 100-101 1.1670110335383796 1107 1 0.4984384775768902 1107 2 0.490151662175613 1107 3 0.6366007401683262 1107 4 0.3600710738103672 1107 5 0.501315567981365 1107 6 0.16876351163501369 1107 7 0.20406598623867467 1107 8 0.06054532589600825 1107 9 0.3511897829354069 1107 10-11 -0.02284976897847457 1107 12-13 -0.26577311759566413 1107 14-15 -0.06504860789716105 1107 16-17 0.07263642382070401 1107 18-19 -0.3675568982011299 1107 20-21 -0.5250714807554075 1107 22-23 -0.09566280218280099 1107 24-25 -0.2625727396963029 1107 26-27 -0.863878226597933 1107 28-29 -0.5956582663481811 1107 30-31 -0.4732916952911026 1107 32-33 0.003663715723924099 1107 34-35 0.19218304896195093 1107 36-37 0.16486348336984946 1107 38-39 -1.4350212572723606 1107 40-41 -2.1359244973424936 1107 42-43 -2.7642974073823083 1107 44-45 -2.8320798142678285 1107 46-47 -2.0723355912478354 1107 48-49 -1.6176108027555394 1107 50-51 -0.9092070240314953 1107 52-53 -0.72078659280022 1107 54-55 -0.8594449370881811 1107 56-57 -0.9060212436838491 1107 58-59 -1.0001308822460366 1107 60-61 -1.330252291507069 1107 62-63 -2.114561248086904 1107 64-65 -1.3091282796003831 1107 66-67 -1.211221368367962 1107 68-69 -1.4932295190473717 1107 70-71 -1.4108686436372082 1107 72-73 -1.8820079462345554 1107 74-75 -2.1016970919416877 1107 76-77 -1.6616570789199052 1107 78-79 -1.3719505973470625 1107 80-81 -1.5722864511643309 1107 82-83 -1.6621397383442549 1107 84-85 -1.205226363136667 1107 86-87 -0.5350913446162835 1107 88-89 -1.3446643747177163 1107 90-91 -1.0981510463575646 1107 92-93 -0.9803789715882765 1107 94-95 -1.0357717766359649 1107 96-97 -1.0347882703497255 1107 98-99 -0.4591838149401788 1107 100-101 -0.2365837377034481 1108 1 -0.5910223067368356 1108 2 -0.2843581417459582 1108 3 0.22606152448204853 1108 4 0.3269828385162441 1108 5 0.22680576405979735 1108 6 0.38322429594873597 1108 7 -0.10230656278093875 1108 8 0.43431983569993093 1108 9 0.3205525280334456 1108 10-11 0.43670905455094555 1108 12-13 0.3598396275023745 1108 14-15 0.23152001955381252 1108 16-17 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-1.5904097805383515 2316 84-85 -1.613764461920482 2316 86-87 -2.1222097362767443 2316 88-89 -3.6441183335164276 2316 90-91 -2.8317077601823364 2316 92-93 -2.76630600062774 2316 94-95 -2.6266131764870444 2316 96-97 -1.5416137853658576 2316 98-99 -1.7019239489684743 2316 100-101 -1.4212449439581007 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 4.0 5 20.0 6 38.0 7 70.0 8 98.0 9 167.0 10 270.0 11 395.0 12 432.0 13 629.0 14 748.0 15 964.0 16 1212.0 17 1562.0 18 1368.0 19 855.0 20 845.0 21 885.0 22 858.0 23 857.0 24 799.0 25 953.0 26 973.0 27 848.0 28 997.0 29 1122.0 30 1235.0 31 1514.0 32 1811.0 33 2246.0 34 2990.0 35 8821.0 36 6010.0 37 7501.0 38 5786.0 39 469.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.20358077203723 23.26078057982879 15.453202584135687 12.082436063998292 2 14.291292463881296 30.307763302687164 35.8311739022399 19.569770331191652 3 22.740581690415773 36.66175713804057 30.156335953720298 10.441325217823364 4 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14.132209405501332 32.941437444543034 36.892635314995566 16.033717834960072 50-51 12.40539125651059 33.84087097718743 37.718387591946836 16.035350174355152 52-53 13.274061454645478 34.734385675371115 34.76810321115163 17.223449658831775 54-55 12.620446116444556 33.21917200503966 35.17736411548631 18.983017763029476 56-57 14.396876802271416 32.65249988909099 34.227407834612485 18.72321547402511 58-59 14.743043725156161 32.60753833049404 34.48857183418512 18.16084611016468 60-61 13.932957126632594 34.19310760931289 34.467277115275415 17.4066581487791 62-63 12.608911681720583 34.22266782602523 34.66718719500293 18.501233297251254 64-65 11.71100030166983 35.27762497116391 33.933419693716395 19.07795503344986 66-67 13.740173548879387 32.99824321686512 32.75779461608078 20.50378861817472 68-69 14.267208489343957 32.97961066846485 32.82167763916739 19.931503203023794 70-71 15.233439213529005 33.56343051833975 31.743651624580764 19.459478643550476 72-73 15.042677408478697 31.790676627686192 33.54213617731088 19.62450978652423 74-75 14.497009919613863 33.88994374744912 32.045321455823114 19.567724877113907 76-77 14.686452507757556 31.94868010420464 32.713321656249164 20.65154573178864 78-79 14.732269755542923 32.01126350198977 33.78872939169983 19.467737350767482 80-81 14.765850975103367 32.16687665252959 32.9636399126932 20.103632459673843 82-83 13.798732986708782 32.003620037974905 33.57585221727326 20.62179475804305 84-85 14.213972636771777 31.96546767696485 32.576792717335366 21.243766968928007 86-87 14.381146121114675 31.908750921640568 32.07486830533619 21.635234651908572 88-89 14.492236301981082 31.195787970729967 31.94895591647332 22.363019810815636 90-91 14.609251250289212 30.33085945147454 32.63032374036699 22.429565557869257 92-93 14.526948878093915 31.466752077439104 32.53705581949857 21.469243224968416 94-95 13.912673132037302 31.012484139730084 33.023078356388034 22.05176437184458 96-97 13.251408544430149 30.97999201455126 33.063306863049554 22.705292577969033 98-99 15.114814026121524 30.9412265758092 30.86580777967064 23.07815161839864 100-101 13.800507515127855 31.426011037566763 30.544070413287667 24.22941103401771 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4305.0 1 2413.0 2 719.5 3 912.5 4 633.0 5 435.0 6 787.5 7 1422.0 8 1808.0 9 1527.5 10 831.5 11 352.0 12 251.5 13 222.0 14 181.0 15 158.0 16 157.0 17 168.0 18 185.0 19 199.5 20 204.0 21 218.0 22 250.0 23 277.0 24 313.5 25 362.0 26 417.0 27 493.0 28 569.0 29 644.0 30 741.5 31 828.5 32 889.5 33 903.5 34 868.0 35 844.5 36 801.5 37 725.5 38 685.5 39 666.5 40 587.5 41 543.0 42 540.5 43 562.5 44 643.5 45 671.5 46 802.0 47 1743.0 48 3867.5 49 4619.5 50 4682.0 51 4034.5 52 1886.5 53 770.5 54 494.0 55 491.0 56 506.5 57 416.0 58 304.0 59 229.0 60 154.5 61 111.0 62 81.0 63 79.5 64 81.5 65 58.0 66 46.5 67 32.0 68 16.0 69 13.0 70 13.5 71 11.0 72 8.0 73 10.5 74 8.0 75 5.0 76 4.0 77 1.5 78 2.0 79 1.0 80 0.0 81 1.0 82 1.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29102266072791155 2 0.01951981260979895 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.872642095363157E-4 26-27 8.872642095363157E-4 28-29 8.872642095363157E-4 30-31 8.872642095363157E-4 32-33 8.872642095363157E-4 34-35 8.872642095363157E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 8.872642095363157E-4 44-45 0.004436321047681579 46-47 0.00798537788582684 48-49 0.005323585257217895 50-51 0.004436321047681579 52-53 0.004436321047681579 54-55 0.0 56-57 8.872642095363157E-4 58-59 0.0017745284190726315 60-61 0.0017745284190726315 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2085070892410342 78-79 0.11534434723972103 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.11978066828740262 88-89 0.57317267936046 90-91 0.2945717175660568 92-93 0.2732773765371852 94-95 0.0026617926286089475 96-97 8.872642095363157E-4 98-99 0.0017745284190726315 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 56353.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.30518339751211 #Duplication Level Percentage of deduplicated Percentage of total 1 83.34885164494104 28.592976416517313 2 7.9764121663563 5.472645644419996 3 2.845023794744465 2.927971891469842 4 1.3966480446927374 1.916490692598442 5 0.8638526794951376 1.4817312299256473 6 0.6517690875232774 1.3415434848189094 7 0.4448582660873164 1.068266108281724 8 0.34657562590523483 0.9511472326229304 9 0.23794744465135526 0.7346547654960695 >10 1.3656114214773432 8.521285468386775 >50 0.25863852679495136 6.260536262488244 >100 0.21208359197186014 15.898000106471704 >500 0.025863852679495137 6.278281546678969 >1k 0.025863852679495137 18.554469149823436 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4337 7.696129753518003 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1893 3.359182297304492 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1773 3.1462388870157754 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1409 2.5003105424733376 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1044 1.8526076695118272 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 894 1.5864284066509324 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 747 1.3255727290472556 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 717 1.2723368764750767 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 613 1.087785920891523 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 567 1.006157613614182 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 500 0.8872642095363157 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 456 0.8091849590971198 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 434 0.770145333877522 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 395 0.7009387255336894 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 351 0.6228594750944936 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 309 0.5483292814934432 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 284 0.5039660710166274 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 263 0.46670097421610207 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 259 0.4596028605398116 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 241 0.4276613489965042 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 236 0.418788706901141 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 233 0.4134651216439232 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 230 0.40814153638670525 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 229 0.40636700796763264 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 227 0.4028179511294873 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 221 0.3921707806150515 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 220 0.3903962521959789 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 219 0.3886217237769063 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 215 0.38152361010061575 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 213 0.3779745532624705 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 212 0.3762000248433979 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 192 0.3407094564619453 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 192 0.3407094564619453 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 191 0.3389349280428726 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 190 0.33716039962379996 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 184 0.3265132291093642 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 184 0.3265132291093642 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 182 0.32296417227121893 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 158 0.2803754902134758 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 155 0.27505190495625786 RNA PCR Primer, Index 33 (95% over 22bp) TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 150 0.2661792628608947 RNA PCR Primer, Index 33 (96% over 29bp) GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 143 0.2537575639273863 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 140 0.24843397867016842 No Hit TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 134 0.2377868081557326 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 129 0.22891416606036946 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 124 0.2200415239650063 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 120 0.2129434102887158 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 118 0.2093943534505705 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 113 0.20052171135520735 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 111 0.1969726545170621 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 102 0.1810018987454084 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 100 0.17745284190726315 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 98 0.17390378506911788 No Hit GTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 96 0.17035472823097264 No Hit ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 95 0.16858019981189998 RNA PCR Primer, Index 33 (95% over 24bp) GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 92 0.1632566145546821 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 91 0.16148208613560947 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 90 0.15970755771653683 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 89 0.1579330292974642 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 88 0.1561585008783916 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 87 0.15438397245931895 No Hit TATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 87 0.15438397245931895 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 86 0.1526094440402463 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 81 0.14373680194488314 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 80 0.14196227352581053 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 77 0.1366386882685926 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 76 0.13486415984952 No Hit GTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 75 0.13308963143044736 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 75 0.13308963143044736 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 72 0.12776604617322945 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 72 0.12776604617322945 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 72 0.12776604617322945 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 71 0.12599151775415685 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 70 0.12421698933508421 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 69 0.12244246091601158 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 69 0.12244246091601158 No Hit GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 69 0.12244246091601158 No Hit ACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 68 0.12066793249693893 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 66 0.11711887565879367 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 66 0.11711887565879367 No Hit GAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 66 0.11711887565879367 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 65 0.11534434723972103 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 64 0.11356981882064841 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 64 0.11356981882064841 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 62 0.11002076198250316 No Hit GGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 59 0.10469717672528525 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 59 0.10469717672528525 No Hit TATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 59 0.10469717672528525 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 58 0.10292264830621262 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 57 0.10114811988713998 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 57 0.10114811988713998 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0017745284190726315 0.0 6 0.0 0.0 0.0 0.0017745284190726315 0.0 7 0.0 0.0 0.0 0.0017745284190726315 0.0 8 0.0 0.0 0.0 0.0017745284190726315 0.0 9 0.0 0.0 0.0 0.0017745284190726315 0.0 10-11 0.0 0.0 0.0 0.002661792628608947 0.0 12-13 0.0 0.0 0.0 0.004436321047681578 0.0 14-15 0.0 0.0 0.0 0.00798537788582684 0.0 16-17 0.0 0.0 0.0 0.010647170514435788 0.0 18-19 0.0 0.0 0.0 0.030166983124234735 0.0 20-21 0.0 0.0 0.0 0.04968679573403368 0.0 22-23 0.0 0.0 0.0 0.10380991251574895 0.0 24-25 0.0 0.0 0.0 0.14906038720210102 0.0 26-27 0.0 0.0 0.0 0.2156052029173247 0.0 28-29 0.0 0.0 0.0 0.3078806807091016 0.0 30-31 0.0 0.0 0.0 0.5509910741220521 0.0 32-33 0.0 0.0 0.0 0.9307401558035951 0.0 34-35 0.0 0.0 0.0 1.4515642468014125 0.0 36-37 0.0 0.0 0.0 2.072649193476834 0.0 38-39 0.0 0.0 0.0 2.8738487746881267 0.0 40-41 0.0 0.0 0.0 3.9110606356360798 0.0 42-43 0.0 0.0 0.0 4.723794651571345 0.0 44-45 0.0 0.0 0.0 5.408762621333381 0.0 46-47 0.0 0.0 0.0 6.352811740280021 0.0 48-49 0.0 0.0 0.0 7.376714638084929 0.0 50-51 0.0 0.0 0.0 8.14952176459106 0.0 52-53 0.0 0.0 0.0 8.673894912427023 0.0 54-55 0.0 0.0 0.0 9.124625130871472 0.0 56-57 0.0 0.0 0.0 9.509697797810231 0.0 58-59 0.0 0.0 0.0 9.87170159530105 0.0 60-61 0.0 0.0 0.0 10.271857753801928 0.0 62-63 0.0 0.0 0.0 10.61789079552109 0.0 64-65 0.0 0.0 0.0 10.920447890972973 0.0 66-67 0.0 0.0 0.0 11.213245080119957 0.0 68-69 0.0 0.0 0.0 11.561939914467729 0.0 70-71 0.0 0.0 0.0 11.943463524568346 0.0 72-73 0.0 0.0 0.0 12.32055081362128 0.0 74-75 0.0 0.0 0.0 12.647064042730644 0.0 76-77 0.0 0.0 0.0 12.966479158163718 0.0 78-79 0.0 0.0 0.0 13.36574805245506 0.0 80-81 0.0 0.0 0.0 13.74638439834614 0.0 82-83 0.0 0.0 0.0 14.09330470427484 0.0 84-85 0.0 0.0 0.0 14.465068408070556 0.0 86-87 0.0 0.0 0.0 14.819086827675545 0.0 88-89 0.0 0.0 0.0 15.202384966195234 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAA 165 7.4578566E-11 31.612246 9 TCCCTCT 35 0.008321496 27.16853 78-79 GAGTATA 35 0.00843049 27.09621 12-13 ATATGGG 35 0.00843049 27.09621 16-17 CGACCCC 45 8.5756904E-4 26.555605 88-89 CAGAGAA 95 1.1885277E-8 24.957035 10-11 CAGAAAA 165 0.0 24.427643 10-11 CATGGGG 180 1.915032E-7 23.709183 4 GCAGAGA 105 0.0021924 22.580175 9 GAGTACT 940 0.0 22.195831 12-13 AGTACTT 900 0.0 20.811396 12-13 GTACTTT 1025 0.0 19.892584 14-15 ACTTTTT 1050 0.0 19.644753 16-17 GCAGAAA 150 7.6484866E-8 18.967346 9 TACTTTT 1020 0.0 18.362993 14-15 GAGAAAA 155 5.5424607E-9 18.355497 12-13 TGGGCTT 285 4.1429303E-8 18.301825 6 AGAGAAA 120 3.0906285E-6 17.781887 10-11 CATGGGC 330 1.1650627E-8 17.243042 4 ATGGGCT 310 1.0830627E-7 16.825872 5 >>END_MODULE