FastQCFastQC Report
Thu 26 May 2016
SRR936313_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936313_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241150
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25320.20400435080369012No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17370.13995085203238933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17700.043.8587071
GTATCAA30150.039.8778461
TCAACGC36000.032.8611534
ATCAACG36550.032.4961243
TATCAAC37750.031.713842
CAACGCA37450.031.462475
AACGCAG38950.030.2508186
GTACCTG12250.027.8215711
ACGCAGA45200.025.4397687
ACATGGG41200.025.1531353
TACATGG42300.025.0583762
TAACGCA950.001254049424.9052934
CGCAGAG46650.024.651028
GTACCGT1601.9221716E-623.660036
ATAACGC1000.001690656323.6600283
GTACATG45950.023.4873811
TACCTGG13200.023.3015442
GTGTACG1652.5045865E-622.9504551
TGTACGC1100.002941562821.5091172
AGTACAT31250.020.8965362
Produced by FastQC (version 0.11.5)