##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936313_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1241150 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.566256294565523 33.0 31.0 34.0 30.0 34.0 2 31.660086210369414 33.0 31.0 34.0 30.0 34.0 3 31.732279740563186 34.0 31.0 34.0 30.0 34.0 4 35.21560004834226 37.0 35.0 37.0 33.0 37.0 5 35.19870362164122 37.0 35.0 37.0 33.0 37.0 6 35.2844394311727 37.0 35.0 37.0 33.0 37.0 7 35.22690972082343 37.0 35.0 37.0 33.0 37.0 8 35.225747089392904 37.0 35.0 37.0 33.0 37.0 9 36.87674253716312 39.0 37.0 39.0 33.0 39.0 10-11 36.79260403657898 39.0 37.0 39.0 33.0 39.0 12-13 36.69056318736655 39.0 37.0 39.0 33.0 39.0 14-15 37.91671715747492 40.0 38.0 41.0 33.0 41.0 16-17 37.85476654715385 40.0 38.0 41.0 33.0 41.0 18-19 37.79739274060347 40.0 38.0 41.0 32.0 41.0 20-21 37.710564395923136 40.0 38.0 41.0 32.0 41.0 22-23 37.546047617129275 40.0 38.0 41.0 32.0 41.0 24-25 37.70880393183741 40.0 38.0 41.0 32.0 41.0 26-27 37.662067034605 40.0 38.0 41.0 32.0 41.0 28-29 37.530715062643516 40.0 38.0 41.0 32.0 41.0 30-31 37.37624904322604 40.0 37.5 41.0 31.5 41.0 32-33 37.19878419207993 40.0 37.0 41.0 31.0 41.0 34-35 36.93046972565766 40.0 37.0 41.0 30.5 41.0 36-37 36.729041211779396 40.0 37.0 41.0 30.0 41.0 38-39 36.57229907746847 40.0 36.0 41.0 30.0 41.0 40-41 36.39224469242235 40.0 36.0 41.0 30.0 41.0 42-43 36.208266124159046 40.0 36.0 41.0 29.5 41.0 44-45 36.02500181283487 40.0 35.0 41.0 28.5 41.0 46-47 35.76354469645087 39.0 35.0 41.0 27.5 41.0 48-49 35.590526930669135 39.0 35.0 41.0 27.0 41.0 50-51 35.294742376022235 38.5 35.0 40.5 26.5 41.0 52-53 35.32407646134634 39.0 35.0 40.5 27.0 41.0 54-55 35.39911291946984 39.0 35.0 41.0 27.0 41.0 56-57 35.23665874390686 39.0 35.0 41.0 26.0 41.0 58-59 34.99773597067236 39.0 35.0 41.0 26.0 41.0 60-61 34.7085863916529 38.0 34.5 40.5 26.0 41.0 62-63 34.40668331789067 38.0 34.0 40.0 25.5 41.0 64-65 34.07619667244089 37.0 34.0 40.0 24.5 41.0 66-67 33.71226644644081 36.5 34.0 40.0 23.0 41.0 68-69 33.324414454336704 36.0 33.0 39.0 22.5 41.0 70-71 32.92152680981348 35.5 33.0 39.0 21.5 41.0 72-73 32.51139709140716 35.0 33.0 38.5 20.5 40.0 74-75 32.08950610321073 35.0 32.0 37.0 19.5 39.5 76-77 31.69144382226161 35.0 32.0 37.0 18.0 39.0 78-79 31.28888047375418 35.0 32.0 36.0 15.0 39.0 80-81 30.889684163880272 35.0 31.0 36.0 10.0 37.5 82-83 30.533459694638037 35.0 31.0 35.5 7.0 37.0 84-85 30.205517866494784 34.5 31.0 35.0 4.5 37.0 86-87 29.87705273335213 34.0 31.0 35.0 2.0 36.0 88-89 29.540936631349958 34.0 30.0 35.0 2.0 36.0 90-91 29.186972968617816 34.0 30.0 35.0 2.0 36.0 92-93 28.863541473633326 34.0 29.5 35.0 2.0 35.0 94-95 28.48633928211739 34.0 29.0 35.0 2.0 35.0 96-97 28.051908713692946 34.0 29.0 35.0 2.0 35.0 98-99 27.545151673850867 34.0 29.0 35.0 2.0 35.0 100-101 24.31201426096765 30.0 14.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2907858419156746 1101 2 0.17549560501978334 1101 3 0.23134466659413278 1101 4 0.1474744639036416 1101 5 0.22066525027923234 1101 6 0.3034956347735758 1101 7 0.3214321951025809 1101 8 0.15373523242401888 1101 9 0.22987069144983963 1101 10-11 0.4611076790153419 1101 12-13 0.266054164580666 1101 14-15 0.4429298273866067 1101 16-17 0.427259788843223 1101 18-19 0.5141576539083985 1101 20-21 0.48570353032432934 1101 22-23 0.534115594567588 1101 24-25 0.4149066295918189 1101 26-27 0.4497938104608892 1101 28-29 0.4141063336703965 1101 30-31 0.5484679484471897 1101 32-33 0.49755128592666154 1101 34-35 0.377763587071712 1101 36-37 0.36600696902984 1101 38-39 0.3035223143882817 1101 40-41 0.30243525602146093 1101 42-43 0.3457776347345103 1101 44-45 0.2046635558293346 1101 46-47 0.30682945750317714 1101 48-49 0.5466985803503377 1101 50-51 0.5013187604791867 1101 52-53 0.5221884810394144 1101 54-55 0.4810888498808694 1101 56-57 0.29442882234461365 1101 58-59 0.40275285187309606 1101 60-61 0.23086800001816954 1101 62-63 0.2637147203803636 1101 64-65 0.21235980554942557 1101 66-67 0.23399008178963498 1101 68-69 0.11933835309043417 1101 70-71 0.4155389155902114 1101 72-73 0.395840500356627 1101 74-75 0.21845277770679417 1101 76-77 0.18304456891670995 1101 78-79 0.3639235067059552 1101 80-81 0.48577959482836874 1101 82-83 0.6041514734058353 1101 84-85 0.2999849759538584 1101 86-87 0.37928680506738743 1101 88-89 0.46634736703606094 1101 90-91 0.5224935321413611 1101 92-93 0.4464280706534076 1101 94-95 0.6820098146749025 1101 96-97 0.6672620679646002 1101 98-99 0.5773255944014686 1101 100-101 0.6900943152101142 1102 1 0.41810327857737306 1102 2 0.38839012397987815 1102 3 0.33700785586388093 1102 4 0.3922798952664479 1102 5 0.3790524004696607 1102 6 0.36749629713489895 1102 7 0.29336629015142535 1102 8 0.4049622567657991 1102 9 0.5042853296349676 1102 10-11 0.5554651885367861 1102 12-13 0.6449645802123953 1102 14-15 0.7993580439787564 1102 16-17 0.6314434285186721 1102 18-19 0.7889655691261481 1102 20-21 0.6533206854824698 1102 22-23 0.5963229136601527 1102 24-25 0.6629775022528577 1102 26-27 0.7356441168030017 1102 28-29 0.5708044624996731 1102 30-31 0.5557481272847369 1102 32-33 0.26077946940074526 1102 34-35 0.43581376094156354 1102 36-37 0.6181281811510573 1102 38-39 0.49152860682080046 1102 40-41 0.45050116097260684 1102 42-43 0.29638617919551535 1102 44-45 0.28855989628305423 1102 46-47 0.3339680566089882 1102 48-49 0.43188569742270033 1102 50-51 0.39545355100742796 1102 52-53 0.40456966997798105 1102 54-55 0.37809828852965666 1102 56-57 0.3907353300445635 1102 58-59 0.4268619759002519 1102 60-61 0.5086830356200949 1102 62-63 0.49914442728548636 1102 64-65 0.38559792444328167 1102 66-67 0.5596151852835973 1102 68-69 0.6052424762896393 1102 70-71 0.5519812073603774 1102 72-73 0.7027174667418024 1102 74-75 0.7810823521396486 1102 76-77 0.5427150441609534 1102 78-79 0.7718768102330316 1102 80-81 0.8394913020646655 1102 82-83 0.7589155071862628 1102 84-85 0.9166971799611439 1102 86-87 0.9691187308829221 1102 88-89 0.7278707980676877 1102 90-91 0.7935127406195832 1102 92-93 0.722153356296726 1102 94-95 0.8267796441168649 1102 96-97 0.8872314337536338 1102 98-99 0.9678902574251431 1102 100-101 0.9484218528819177 1103 1 0.2294780269236476 1103 2 0.21307136259554227 1103 3 0.29856954697690696 1103 4 0.23964352291480395 1103 5 0.10822505889167644 1103 6 -0.02146449281812579 1103 7 0.10329822381071097 1103 8 0.24939711440169532 1103 9 0.11483081904154346 1103 10-11 0.34129109209349906 1103 12-13 0.4187973862712582 1103 14-15 0.4269968130325381 1103 16-17 0.4721082736917097 1103 18-19 0.19592798883662965 1103 20-21 0.40335903271667206 1103 22-23 0.418700921521328 1103 24-25 0.500313328156416 1103 26-27 0.5350808918006038 1103 28-29 0.4539388695555644 1103 30-31 0.42270239820795297 1103 32-33 0.37694522532059693 1103 34-35 0.23146374656135293 1103 36-37 0.23260186536158045 1103 38-39 0.3779800496354895 1103 40-41 0.4085516834536804 1103 42-43 0.23712532213482973 1103 44-45 0.34601124322964694 1103 46-47 0.3985280220964782 1103 48-49 0.29344499183359574 1103 50-51 0.3844766552160337 1103 52-53 0.6435999642930099 1103 54-55 0.6566789615236175 1103 56-57 0.5739104810367905 1103 58-59 0.6563174451745297 1103 60-61 0.4595362615811709 1103 62-63 0.2807880856116185 1103 64-65 0.44366762054144715 1103 66-67 0.32063282501133017 1103 68-69 0.38692208514784454 1103 70-71 0.32187862850887683 1103 72-73 0.28792981455120525 1103 74-75 0.27078132635113406 1103 76-77 0.26605892298369227 1103 78-79 0.24931425630723325 1103 80-81 0.19410495368004277 1103 82-83 0.4356267525126931 1103 84-85 0.5486691864801756 1103 86-87 0.541384093743627 1103 88-89 0.34349250260224906 1103 90-91 0.5118954460169576 1103 92-93 0.3293866033487838 1103 94-95 0.40468924051222643 1103 96-97 0.3115363901336572 1103 98-99 0.2099571733488368 1103 100-101 0.31081999304105423 1104 1 0.22241382386280506 1104 2 0.16874512146078757 1104 3 0.2435303532479054 1104 4 0.26259051806224676 1104 5 0.2563680226841356 1104 6 0.2708554026652621 1104 7 0.23316011257517744 1104 8 0.27176553545432114 1104 9 0.31470950511843654 1104 10-11 0.24571438436673532 1104 12-13 0.26373952886176966 1104 14-15 0.33520416652265084 1104 16-17 0.3920550822023472 1104 18-19 0.41500227028492986 1104 20-21 0.37257329176856047 1104 22-23 0.43061301558628173 1104 24-25 0.40307555286712926 1104 26-27 0.3584011605575981 1104 28-29 0.4435711950501471 1104 30-31 0.31849373826020866 1104 32-33 0.4108104735475493 1104 34-35 0.35396281337732916 1104 36-37 0.48116009604466825 1104 38-39 0.33274861999756666 1104 40-41 0.3967888988325541 1104 42-43 0.44262644195565315 1104 44-45 0.5591364765256586 1104 46-47 0.6670470590505673 1104 48-49 0.7261344281904343 1104 50-51 0.5065137959401866 1104 52-53 0.4322384488021527 1104 54-55 0.5756552586494124 1104 56-57 0.6553121234406802 1104 58-59 0.5170210659285459 1104 60-61 0.4861226744217859 1104 62-63 0.5088162677691486 1104 64-65 0.559419766864707 1104 66-67 0.5602231572248471 1104 68-69 0.5587725890672246 1104 70-71 0.46040513093515045 1104 72-73 0.5542512031290414 1104 74-75 0.4691249247087228 1104 76-77 0.5776770640811186 1104 78-79 0.4636059696331003 1104 80-81 0.732449485221661 1104 82-83 0.8235373534832071 1104 84-85 0.5844707915566971 1104 86-87 0.5349637876592652 1104 88-89 0.6125014611688755 1104 90-91 0.7604303470994509 1104 92-93 0.7022356141737127 1104 94-95 0.534490472315138 1104 96-97 0.7839561363076015 1104 98-99 0.6533362326993419 1104 100-101 0.7206697504132116 1105 1 0.443294774031596 1105 2 0.40470163916031865 1105 3 0.4432144561931821 1105 4 0.3213450474685473 1105 5 0.2831503634193666 1105 6 0.40599662723090546 1105 7 0.45625156786955046 1105 8 0.3903608775212817 1105 9 0.40374524484403906 1105 10-11 0.524337135148734 1105 12-13 0.48004384302448955 1105 14-15 0.6583804030118543 1105 16-17 0.6817516506931653 1105 18-19 0.60531088931927 1105 20-21 0.6540600209952245 1105 22-23 0.7045681551074807 1105 24-25 0.6436481937045642 1105 26-27 0.5317310871579934 1105 28-29 0.5051742177513745 1105 30-31 0.6931344827862134 1105 32-33 0.5086410040687781 1105 34-35 0.44795612379262906 1105 36-37 0.4735139162311057 1105 38-39 0.5651420047415954 1105 40-41 0.5906726518134349 1105 42-43 0.7033897824121595 1105 44-45 0.4558346554720458 1105 46-47 0.3909263213380285 1105 48-49 0.5184909582780861 1105 50-51 0.5785240006538643 1105 52-53 0.6835620419762947 1105 54-55 0.6949594334457814 1105 56-57 0.6590556583906633 1105 58-59 0.7041800055054921 1105 60-61 0.6938195681007429 1105 62-63 0.5058187299079577 1105 64-65 0.5856368996343804 1105 66-67 0.6031445081493061 1105 68-69 0.6110314852857428 1105 70-71 0.591253484863735 1105 72-73 0.7339230728060215 1105 74-75 0.7081788594852796 1105 76-77 0.6654383759830047 1105 78-79 0.7571668572418844 1105 80-81 0.6655195710093942 1105 82-83 0.6764698537154814 1105 84-85 0.6088277707136847 1105 86-87 0.48732372447985384 1105 88-89 0.3851802372226416 1105 90-91 0.424214453141758 1105 92-93 0.45752572845411876 1105 94-95 0.5306660274577553 1105 96-97 0.6149972803504653 1105 98-99 0.6405133673272339 1105 100-101 0.7597608495491421 1106 1 0.3770771284837764 1106 2 0.3595534848562103 1106 3 0.4713887056001127 1106 4 0.4811265557564255 1106 5 0.38895716597599517 1106 6 0.3842600260916029 1106 7 0.3758077849106982 1106 8 0.4143549241509774 1106 9 0.4404211790245469 1106 10-11 0.3833159206007508 1106 12-13 0.45530864712234376 1106 14-15 0.6237005099911954 1106 16-17 0.548867212561376 1106 18-19 0.49885095368819776 1106 20-21 0.47642860110129703 1106 22-23 0.4943216342026915 1106 24-25 0.49346120650280056 1106 26-27 0.4098441407534352 1106 28-29 0.4266259939409167 1106 30-31 0.4067535728573759 1106 32-33 0.4887387662663869 1106 34-35 0.1859862986162213 1106 36-37 0.18113279476120425 1106 38-39 0.2896248623593394 1106 40-41 0.42731572157666164 1106 42-43 0.35658291941523146 1106 44-45 0.45050344258617514 1106 46-47 0.5465793788620914 1106 48-49 0.630441895858354 1106 50-51 0.629969279510334 1106 52-53 0.5110057191646149 1106 54-55 0.4009347133599519 1106 56-57 0.5748377559715436 1106 58-59 0.6266188474691958 1106 60-61 0.4720916552556744 1106 62-63 0.5590859062878835 1106 64-65 0.169078899604159 1106 66-67 0.3909812739884373 1106 68-69 0.45269790011779776 1106 70-71 0.520398934464076 1106 72-73 0.8165797265283885 1106 74-75 0.6008198742780451 1106 76-77 0.54661801968739 1106 78-79 0.5623522578855713 1106 80-81 0.5541076854228955 1106 82-83 0.6235585196467923 1106 84-85 0.5480818617670096 1106 86-87 0.4893276984300847 1106 88-89 0.3523539728869558 1106 90-91 0.2965293924747989 1106 92-93 0.3236787784231474 1106 94-95 0.38849455830497703 1106 96-97 0.45684841587274505 1106 98-99 0.6893325148166944 1106 100-101 0.6727744032881269 1107 1 0.030956037017375593 1107 2 0.083619307801019 1107 3 0.23960535567674413 1107 4 0.10089081923780441 1107 5 0.233699704409581 1107 6 0.27535117649213703 1107 7 0.16523045164056072 1107 8 0.29630891444976015 1107 9 0.25340327758516423 1107 10-11 0.12961120745038102 1107 12-13 0.1702633800227531 1107 14-15 0.3001402881587083 1107 16-17 0.21423156136294352 1107 18-19 0.200319961920016 1107 20-21 0.47004973206778544 1107 22-23 0.3233933031603655 1107 24-25 0.3797053012398095 1107 26-27 0.4360121583230736 1107 28-29 0.5343221908725511 1107 30-31 0.38406985795320026 1107 32-33 0.393269426233843 1107 34-35 0.1406382238504591 1107 36-37 0.452603066995799 1107 38-39 0.5134102346871572 1107 40-41 0.6434771821318748 1107 42-43 0.22538535578058827 1107 44-45 0.5266228750489219 1107 46-47 0.6070536169054179 1107 48-49 0.5011174263445213 1107 50-51 0.4605881668718794 1107 52-53 0.4707148405246855 1107 54-55 0.653044018000422 1107 56-57 0.5269506156198247 1107 58-59 0.7081679618772512 1107 60-61 0.5989979294494745 1107 62-63 0.7306138486493552 1107 64-65 0.5642215739180401 1107 66-67 0.624940683699144 1107 68-69 0.6892532555395761 1107 70-71 0.6968485876533066 1107 72-73 0.6107969138061975 1107 74-75 0.7165515738877843 1107 76-77 0.676465075350908 1107 78-79 0.8201626100683512 1107 80-81 0.7740797193613709 1107 82-83 1.0553635946179547 1107 84-85 0.8446196791502061 1107 86-87 0.5825640985501614 1107 88-89 0.7776549635491357 1107 90-91 1.0233590365000182 1107 92-93 0.6714197684533225 1107 94-95 0.6328255058330647 1107 96-97 0.9593313913352901 1107 98-99 0.8963666902918774 1107 100-101 0.6602852658120248 1108 1 -0.18148688535705304 1108 2 -0.048595304071124445 1108 3 -0.08274624249677487 1108 4 -0.022374020944845086 1108 5 -0.04221353759955093 1108 6 -0.14870133492338766 1108 7 -0.08871932004893779 1108 8 -0.1607344645456763 1108 9 -0.05043233885319154 1108 10-11 -0.06699838159071447 1108 12-13 -0.1440973505708527 1108 14-15 -0.10199441503763751 1108 16-17 -0.026641726308291425 1108 18-19 -0.025804467303728984 1108 20-21 0.053052015172809774 1108 22-23 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2316 48-49 -2.06927316571894 2316 50-51 -2.1296565321085836 2316 52-53 -2.024069941942024 2316 54-55 -1.9689655300694753 2316 56-57 -2.056062370467778 2316 58-59 -2.248305783899454 2316 60-61 -2.153695778206206 2316 62-63 -2.204684617602851 2316 64-65 -2.119359664837148 2316 66-67 -2.188287965260841 2316 68-69 -2.363091603169174 2316 70-71 -2.18308680849065 2316 72-73 -2.127078284369695 2316 74-75 -2.0289677211701402 2316 76-77 -2.142756353178104 2316 78-79 -2.0513998175640538 2316 80-81 -2.1083195894723765 2316 82-83 -2.136335581801635 2316 84-85 -2.2844428523946476 2316 86-87 -2.1517044468536426 2316 88-89 -2.047597543709891 2316 90-91 -2.3388984763702823 2316 92-93 -2.4270788450576717 2316 94-95 -2.2956512563736844 2316 96-97 -2.4554776175774293 2316 98-99 -2.3156913106700543 2316 100-101 -2.1918669095209395 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13407.0 3 2769.0 4 2160.0 5 1956.0 6 1740.0 7 1851.0 8 2251.0 9 2727.0 10 3154.0 11 3861.0 12 4625.0 13 6499.0 14 6772.0 15 5904.0 16 6698.0 17 8055.0 18 7527.0 19 6143.0 20 5979.0 21 6244.0 22 6501.0 23 7245.0 24 8317.0 25 9492.0 26 11206.0 27 13377.0 28 15841.0 29 19159.0 30 23229.0 31 28729.0 32 36379.0 33 47069.0 34 65351.0 35 96645.0 36 159657.0 37 267559.0 38 270049.0 39 54930.0 40 93.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.395480886346654 17.635361004326295 12.761647426591205 24.207510682735844 2 15.595109742320293 18.749909347154468 40.83084743085807 24.82413347966717 3 20.477993908236773 22.952651850896842 27.753219126202637 28.816135114663744 4 12.636745928341387 14.857635976436633 36.14596653395956 36.35965156126242 5 14.147432204311688 36.88214367730421 33.97902574040425 14.991398377979849 6 34.69116265428169 34.51969826134461 16.72559412251002 14.063544961863675 7 30.587819147693256 30.949037087888847 19.65555778479855 18.807585979619347 8 26.992247524824275 35.93704548586126 18.621733843772436 18.448973145542023 9 26.55176109482893 16.400193076172418 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34-35 23.226568978756596 26.352105456409436 27.57643582121626 22.844889743617706 36-37 23.357625823332448 26.06932022046204 27.56645758178274 23.006596374422777 38-39 23.08837815520619 26.316322069847644 27.548006087787186 23.04729368715898 40-41 23.365905242949253 26.179361965197074 27.598175238925 22.856557552928678 42-43 23.194023325385484 26.159124306249925 27.431292935590037 23.215559432774555 44-45 23.094519632258777 26.4622823130918 27.42564544398554 23.01755261066388 46-47 23.295877389820653 26.474422875874687 27.119307005659692 23.11039272864497 48-49 23.383323709416565 26.470657312165574 27.14689606412849 22.999122914289373 50-51 23.27759018540462 26.729820815760892 26.961910693791204 23.03067830504329 52-53 23.431334398823452 26.668418638005605 26.90987484202901 22.990372121141935 54-55 23.325148133227795 26.57531362542284 26.986382067326474 23.113156174022887 56-57 23.196516959740503 26.927935171532607 26.85688328786823 23.018664580858665 58-59 23.34111859138245 26.621938881473394 26.969137909186564 23.06780461795759 60-61 23.177571455521917 26.648673070087582 27.077717925071394 23.096037549319107 62-63 23.205278302704084 26.648336078063693 27.225134281277374 22.921251337954843 64-65 23.119063014592722 26.730661233552773 27.0599195965006 23.090356155353913 66-67 23.107350235684375 26.87939315068216 26.878016891556854 23.135239722076616 68-69 23.233912126310077 26.658690296158184 27.02272860703014 23.0846689705016 70-71 23.10762971733916 26.472477760573216 27.035856331784924 23.384036190302698 72-73 23.185080164239324 26.722573676526913 26.920990391303185 23.171355767930578 74-75 23.07827172101095 26.94604280571418 26.788048995087294 23.18763647818758 76-77 23.21492769347865 26.779323411766608 26.773979368755164 23.231769525999578 78-79 23.300349834799107 26.81311064714782 26.748819747832307 23.137719770220762 80-81 23.173706441607635 26.89667848723213 26.671819186239293 23.257795884920945 82-83 23.34027542168733 26.97470969141985 26.773410721963693 22.91160416492912 84-85 23.351100116803273 26.98548359997004 26.545558337871185 23.117857945355503 86-87 23.19671274939208 26.758670168014493 26.905882248150338 23.138734834443092 88-89 23.33074315664927 26.843719212969617 26.624654243806756 23.200883386574354 90-91 23.14001620089105 27.07245848521669 26.64787363304982 23.139651680842448 92-93 23.196474774722603 26.897783127045884 26.74846889124764 23.157273206983874 94-95 23.180548031944916 26.900706962990313 26.75046416966464 23.168280835400136 96-97 23.30400100702122 26.756529549070628 26.74365845790566 23.195810986002485 98-99 23.091514388209696 26.826289240571903 26.636764077393288 23.445432293825114 100-101 23.230527628021434 26.973748117927048 26.385853287354088 23.409870966697426 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 47.0 1 42.0 2 84.5 3 147.0 4 130.5 5 125.5 6 239.0 7 455.5 8 731.0 9 884.5 10 815.0 11 771.0 12 865.5 13 987.0 14 1095.0 15 1213.5 16 1311.5 17 1399.0 18 1510.5 19 1618.0 20 1710.5 21 1851.5 22 2134.5 23 2536.0 24 3160.5 25 4018.0 26 5112.0 27 6630.0 28 8223.0 29 9764.0 30 11429.0 31 13699.0 32 16293.0 33 18324.0 34 20288.5 35 22474.5 36 25154.0 37 27725.0 38 29643.5 39 31450.5 40 33594.0 41 35990.0 42 37757.0 43 39232.5 44 41220.0 45 43452.0 46 45019.5 47 46310.0 48 47741.0 49 48217.0 50 47504.0 51 47075.0 52 47145.5 53 46969.5 54 46863.0 55 44568.0 56 40665.5 57 37291.5 58 34435.0 59 31059.5 60 26764.0 61 22601.0 62 18371.0 63 14228.5 64 10565.5 65 7906.5 66 5840.5 67 4420.0 68 3360.0 69 2463.0 70 1857.0 71 1256.5 72 866.0 73 699.5 74 549.5 75 386.5 76 253.5 77 143.0 78 90.5 79 52.5 80 40.5 81 33.5 82 26.0 83 19.0 84 10.5 85 10.5 86 14.5 87 14.0 88 9.5 89 9.5 90 12.0 91 10.0 92 9.5 93 11.0 94 12.5 95 10.5 96 8.5 97 12.0 98 9.5 99 4.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04810055190750513 2 0.01224670668331789 3 0.010474157031785038 4 0.005720501148128752 5 0.00845989606413407 6 0.006123353341658945 7 0.005720501148128752 8 0.010232445715666921 9 0.015227812915441324 10-11 0.002215687064416066 12-13 0.00910445957378238 14-15 0.009305885670547477 16-17 0.019820327921685532 18-19 0.011561857954316562 20-21 0.003988236715948918 22-23 0.009950449180195785 24-25 0.0220360149861016 26-27 0.007211054264190469 28-29 0.017644926076622488 30-31 0.01486524594126415 32-33 0.012730129315554123 34-35 0.2683398461104621 36-37 0.4774604197719857 38-39 0.4745598839785683 40-41 0.4946219232163719 42-43 0.48463118881682304 44-45 0.49997985739032347 46-47 0.5057406437578053 48-49 0.5139588285058212 50-51 0.5236272811505458 52-53 0.5116625710026991 54-55 0.49933529388067516 56-57 0.4944607823389598 58-59 0.49337308141642827 60-61 0.4899488377714216 62-63 0.48793457680377067 64-65 0.5038472384482133 66-67 0.47677557104298435 68-69 0.5037666680095073 70-71 0.48442976272005794 72-73 0.49333279619707526 74-75 0.5069089151190428 76-77 0.4938967892680175 78-79 0.4945816379970189 80-81 0.4959513354550215 82-83 0.4972001772549651 84-85 0.496918180719494 86-87 0.49929500866132215 88-89 0.5121459936349353 90-91 0.5357934173951577 92-93 0.5241509890021351 94-95 0.4954276276034323 96-97 0.4692019498046167 98-99 0.4587680779921847 100-101 0.4681142488820852 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1241150.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.60478246821731 #Duplication Level Percentage of deduplicated Percentage of total 1 82.52158886816132 56.6137565323187 2 10.902659671093911 14.959491901208072 3 3.092661283572893 6.365140638221881 4 1.2033848308558988 3.3023181818568568 5 0.6251707633675136 2.1444852113158817 6 0.3731598226609403 1.5360329075719414 7 0.23760518340689674 1.1410596344665718 8 0.1778936973482661 0.9763486727235746 9 0.13876993235456456 0.8568252920082721 >10 0.6752957850350056 8.1778793945782 >50 0.03700637768221998 1.691041102016513 >100 0.01421633892650427 1.7096043999267612 >500 2.349782136228313E-4 0.08245701851331513 >1k 3.524673204342469E-4 0.443559113273483 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2532 0.20400435080369012 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1737 0.13995085203238933 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.057043870603875E-5 2 0.0 0.0 0.0 0.0 8.057043870603875E-5 3 0.0 0.0 0.0 0.0 8.057043870603875E-5 4 0.0 0.0 0.0 0.0 8.057043870603875E-5 5 0.0 0.0 0.0 0.0 8.057043870603875E-5 6 0.0 0.0 0.0 0.0 8.057043870603875E-5 7 0.0 0.0 0.0 0.0 8.057043870603875E-5 8 0.0 0.0 0.0 0.0 8.057043870603875E-5 9 0.0 0.0 0.0 0.0 8.057043870603875E-5 10-11 0.0 0.0 0.0 1.611408774120775E-4 8.057043870603875E-5 12-13 0.0 0.0 0.0 2.819965354711356E-4 1.611408774120775E-4 14-15 0.0 0.0 0.0 3.625669741771744E-4 2.0142609676509688E-4 16-17 0.0 0.0 0.0 4.834226322362325E-4 2.4171131611811625E-4 18-19 0.0 0.0 0.0 4.834226322362325E-4 2.4171131611811625E-4 20-21 0.0 0.0 0.0 4.834226322362325E-4 2.4171131611811625E-4 22-23 0.0 0.0 0.0 5.639930709422712E-4 2.819965354711356E-4 24-25 0.0 0.0 0.0 7.251339483543488E-4 3.22281754824155E-4 26-27 0.0 0.0 0.0 8.057043870603875E-4 3.22281754824155E-4 28-29 0.0 0.0 0.0 0.0010877009225315232 3.22281754824155E-4 30-31 0.0 0.0 0.0 0.002014260967650969 3.22281754824155E-4 32-33 0.0 0.0 0.0 0.003947951496595899 3.22281754824155E-4 34-35 0.0 0.0 0.0 0.006767916851307255 3.22281754824155E-4 36-37 0.0 0.0 0.0 0.011561857954316562 3.625669741771744E-4 38-39 0.0 0.0 0.0 0.019659187044273455 4.0285219353019375E-4 40-41 0.0 0.0 0.0 0.03130161543729606 4.0285219353019375E-4 42-43 0.0 0.0 0.0 0.042299480320670345 4.0285219353019375E-4 44-45 0.0 0.0 0.0 0.05482818353945937 4.0285219353019375E-4 46-47 0.0 0.0 0.0 0.06896829553236918 4.0285219353019375E-4 48-49 0.0 0.0 0.0 0.08447810498328162 4.834226322362325E-4 50-51 0.0 0.0 0.0 0.10526527816943963 4.834226322362325E-4 52-53 0.0 0.0 0.0 0.12508560609112518 4.834226322362325E-4 54-55 0.0 0.0 0.0 0.14591306449663619 4.834226322362325E-4 56-57 0.0 0.0 0.0 0.17125246746968537 4.834226322362325E-4 58-59 0.0 0.0 0.0 0.19949240623615194 4.834226322362325E-4 60-61 0.0 0.0 0.0 0.23188172259597956 4.834226322362325E-4 62-63 0.0 0.0 0.0 0.26600330338798694 4.834226322362325E-4 64-65 0.0 0.0 0.0 0.3044354026507674 4.834226322362325E-4 66-67 0.0 0.0 0.0 0.3461306046811425 4.834226322362325E-4 68-69 0.0 0.0 0.0 0.3941103009305886 4.834226322362325E-4 70-71 0.0 0.0 0.0 0.4471659348185151 4.834226322362325E-4 72-73 0.0 0.0 0.0 0.503202674938565 5.237078515892519E-4 74-75 0.0 0.0 0.0 0.5615759577810902 5.639930709422713E-4 76-77 0.0 0.0 0.0 0.6272811505458648 5.639930709422713E-4 78-79 0.0 0.0 0.0 0.7063610361358418 5.639930709422713E-4 80-81 0.0 0.0 0.0 0.7930145429641864 5.639930709422713E-4 82-83 0.0 0.0 0.0 0.887644523224429 6.4456350964831E-4 84-85 0.0 0.0 0.0 0.9828787817749668 6.4456350964831E-4 86-87 0.0 0.0 0.0 1.084921242396165 6.4456350964831E-4 88-89 0.0 0.0 0.0 1.2051726221649277 6.848487290013294E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1770 0.0 43.858707 1 GTATCAA 3015 0.0 39.877846 1 TCAACGC 3600 0.0 32.861153 4 ATCAACG 3655 0.0 32.496124 3 TATCAAC 3775 0.0 31.71384 2 CAACGCA 3745 0.0 31.46247 5 AACGCAG 3895 0.0 30.250818 6 GTACCTG 1225 0.0 27.821571 1 ACGCAGA 4520 0.0 25.439768 7 ACATGGG 4120 0.0 25.153135 3 TACATGG 4230 0.0 25.058376 2 TAACGCA 95 0.0012540494 24.905293 4 CGCAGAG 4665 0.0 24.65102 8 GTACCGT 160 1.9221716E-6 23.66003 6 ATAACGC 100 0.0016906563 23.660028 3 GTACATG 4595 0.0 23.487381 1 TACCTGG 1320 0.0 23.301544 2 GTGTACG 165 2.5045865E-6 22.950455 1 TGTACGC 110 0.0029415628 21.509117 2 AGTACAT 3125 0.0 20.896536 2 >>END_MODULE