FastQCFastQC Report
Thu 26 May 2016
SRR936313_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936313_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241150
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26980.21737904362889257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20180.1625911453087862No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13270.10691697216291343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC751.3775752E-744.2730753
GGTATCA19450.043.038021
CTATACG500.00162669837.9483534
GTATCAA31400.037.413341
TACCTGG11150.035.3192252
TAACGCA1001.3131848E-633.2048074
GTACCTG12450.032.089961
TAGCGCG600.003977463631.6236294
ATCAACG37550.030.8235633
TCAACGC38250.030.383494
CAACGCA39100.029.6016585
TATCAAC39500.029.5492152
AACGCAG41000.028.2298746
GTGTAGC8250.027.095871
TATACCG909.019281E-426.3530245
TACATGG41550.025.122312
GTACATG42900.024.8341871
ACGCAGA48300.023.9632477
ACATGGG42200.023.8301283
CGTAAAG1201.7267717E-423.717723
Produced by FastQC (version 0.11.5)