##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936313_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1241150 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.448603311445034 34.0 31.0 34.0 31.0 34.0 2 32.583508036901264 34.0 31.0 34.0 31.0 34.0 3 32.67183257462837 34.0 31.0 34.0 31.0 34.0 4 36.16507271482093 37.0 35.0 37.0 35.0 37.0 5 36.12412601216614 37.0 35.0 37.0 35.0 37.0 6 36.157378237924505 37.0 36.0 37.0 35.0 37.0 7 36.109793336824715 37.0 36.0 37.0 35.0 37.0 8 36.08680256213995 37.0 36.0 37.0 35.0 37.0 9 37.855123071345126 39.0 38.0 39.0 35.0 39.0 10-11 37.78714619506103 39.0 38.0 39.0 35.0 39.0 12-13 37.73050678805946 39.0 38.0 39.0 35.0 39.0 14-15 39.07549168110221 40.0 38.0 41.0 36.0 41.0 16-17 38.997946259517384 40.0 38.0 41.0 35.5 41.0 18-19 39.012671715747494 40.0 38.5 41.0 35.0 41.0 20-21 38.96139668855497 40.0 38.0 41.0 35.0 41.0 22-23 38.837931756838415 40.0 38.0 41.0 35.0 41.0 24-25 39.006301414011205 40.0 38.5 41.0 35.0 41.0 26-27 38.93107158683479 40.0 38.0 41.0 35.0 41.0 28-29 38.83785118639971 40.0 38.0 41.0 35.0 41.0 30-31 38.72374088546912 40.0 38.0 41.0 35.0 41.0 32-33 38.57822382467873 40.0 38.0 41.0 34.0 41.0 34-35 38.44353543085042 40.0 38.0 41.0 34.0 41.0 36-37 38.30505942069855 40.0 38.0 41.0 34.0 41.0 38-39 38.17026346533457 40.0 38.0 41.0 33.5 41.0 40-41 38.032206018611774 40.0 38.0 41.0 33.0 41.0 42-43 38.06479353825082 40.0 38.0 41.0 33.0 41.0 44-45 37.940464085726944 40.0 38.0 41.0 33.0 41.0 46-47 37.75013817830238 40.0 37.0 41.0 33.0 41.0 48-49 37.52253514885388 40.0 37.0 41.0 32.5 41.0 50-51 37.32778753575313 40.0 37.0 41.0 32.0 41.0 52-53 37.115926761471215 40.0 36.0 41.0 31.5 41.0 54-55 36.91583813398864 39.5 36.0 41.0 31.0 41.0 56-57 36.66231922007815 39.0 35.5 41.0 31.0 41.0 58-59 36.3958030858478 39.0 35.0 41.0 30.5 41.0 60-61 36.1090847198163 38.5 35.0 40.5 30.0 41.0 62-63 35.80099262780486 38.0 35.0 40.0 29.5 41.0 64-65 35.42849856987471 37.5 35.0 40.0 29.0 41.0 66-67 35.07634854771784 37.0 34.0 40.0 29.0 41.0 68-69 34.71100914474479 36.0 34.0 39.0 28.0 41.0 70-71 34.329857793175684 36.0 34.0 39.0 28.0 40.5 72-73 33.91648310035048 35.0 34.0 38.5 27.5 40.0 74-75 33.47650968859526 35.0 33.0 37.0 26.5 39.5 76-77 32.76076179349796 35.0 32.5 37.0 26.0 39.0 78-79 32.814609837650565 35.0 33.0 36.0 26.0 39.0 80-81 32.640800467308544 35.0 33.0 36.0 26.0 38.0 82-83 32.34499254723442 35.0 33.0 36.0 26.0 37.0 84-85 32.03755347862869 35.0 33.0 35.0 25.5 37.0 86-87 31.611613423035088 35.0 32.0 35.0 25.0 36.0 88-89 31.105954558272572 34.5 32.0 35.0 23.0 36.0 90-91 30.87088869193893 34.0 31.5 35.0 20.5 36.0 92-93 30.569251903476612 34.0 31.0 35.0 19.5 35.0 94-95 30.382412278934858 34.0 31.0 35.0 18.5 35.0 96-97 30.10472223341256 34.0 31.0 35.0 12.5 35.0 98-99 29.603028239938766 34.0 31.0 35.0 2.0 35.0 100-101 26.153243765862307 30.5 24.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10682395494345798 1101 2 0.11731914598938431 1101 3 0.15234973980260236 1101 4 0.13101260980871388 1101 5 0.12534908990357962 1101 6 0.1445447062073555 1101 7 0.19550411140235013 1101 8 0.06497698403877195 1101 9 0.10816482448944242 1101 10-11 0.08887419816998943 1101 12-13 0.17708577680632231 1101 14-15 0.3627980599028007 1101 16-17 0.44684586356631684 1101 18-19 0.326099892458906 1101 20-21 0.17784781933055882 1101 22-23 0.17859922790243132 1101 24-25 0.15782643993607337 1101 26-27 0.3279317549126759 1101 28-29 0.35853764963378154 1101 30-31 0.2694476622267743 1101 32-33 0.5127044941821666 1101 34-35 0.44681908195343567 1101 36-37 0.4841545921356314 1101 38-39 0.3501305766127558 1101 40-41 0.28374665126020915 1101 42-43 0.3703805224158714 1101 44-45 0.5040489499220655 1101 46-47 0.4459087944791804 1101 48-49 0.2854094939399303 1101 50-51 0.25304779803276745 1101 52-53 0.33855501641740915 1101 54-55 0.30267446852491986 1101 56-57 0.24046667339877104 1101 58-59 0.17294476240194 1101 60-61 0.287438123532894 1101 62-63 0.3637831616642515 1101 64-65 0.3449975463476349 1101 66-67 0.3247889859109705 1101 68-69 0.14262440156149125 1101 70-71 0.2786033200423219 1101 72-73 0.0979339746661978 1101 74-75 0.2667213306918441 1101 76-77 0.015886709443741154 1101 78-79 0.07235912084808405 1101 80-81 0.19971091670937824 1101 82-83 0.10723423764456186 1101 84-85 0.20659540539973165 1101 86-87 0.20778107181569894 1101 88-89 0.19543113802718182 1101 90-91 0.3404486166157028 1101 92-93 0.25450654679582385 1101 94-95 0.3527697287530742 1101 96-97 0.2770336790971015 1101 98-99 0.3450950485013564 1101 100-101 0.6197231604058153 1102 1 0.13428214338524924 1102 2 0.18965728144227967 1102 3 0.2258188572061428 1102 4 0.14341698139340764 1102 5 0.12674501638147717 1102 6 0.17791115760659437 1102 7 0.19243572259625807 1102 8 0.23105083732573917 1102 9 0.2192477852445336 1102 10-11 0.29224147752087504 1102 12-13 0.35864894951703263 1102 14-15 0.2871646768923668 1102 16-17 0.3602661318226481 1102 18-19 0.4205666915978341 1102 20-21 0.3325398213176456 1102 22-23 0.3631082525753939 1102 24-25 0.21918842039642072 1102 26-27 0.35531956746442717 1102 28-29 0.3534747253085655 1102 30-31 0.33140990763164524 1102 32-33 0.35355066076603947 1102 34-35 0.4320856823839634 1102 36-37 0.3207939363979264 1102 38-39 0.4676906031072008 1102 40-41 0.3189776497698986 1102 42-43 0.3024495735832886 1102 44-45 0.32045150001314227 1102 46-47 0.3032866716111542 1102 48-49 0.24636495014129167 1102 50-51 0.26461097074348316 1102 52-53 0.15999813613921532 1102 54-55 -0.013375374164262155 1102 56-57 0.14648919368358548 1102 58-59 0.02964040737627016 1102 60-61 0.2116739241621417 1102 62-63 0.24799660759899922 1102 64-65 0.31957694691063665 1102 66-67 0.29731092662962766 1102 68-69 0.02123592664842988 1102 70-71 0.17274225032878832 1102 72-73 0.18864104537326654 1102 74-75 0.13563919788839485 1102 76-77 -0.014529750235006134 1102 78-79 0.0990547658224159 1102 80-81 0.11747958701903372 1102 82-83 0.18547732424831054 1102 84-85 0.2944750212301628 1102 86-87 0.13870092609620954 1102 88-89 0.3412557778682199 1102 90-91 0.4756431852528955 1102 92-93 0.46412651698956253 1102 94-95 0.3222497751183617 1102 96-97 0.22950925452349935 1102 98-99 0.11326693126340359 1102 100-101 0.6125622066055172 1103 1 0.035787272327823416 1103 2 0.035261729721440815 1103 3 0.060786741397500066 1103 4 0.044696820335026644 1103 5 0.008985453539942512 1103 6 0.01759095820097656 1103 7 0.12654079401798413 1103 8 0.11411574001963487 1103 9 0.12449656292644562 1103 10-11 0.12795713277923682 1103 12-13 0.1037285200280067 1103 14-15 0.1552621747353342 1103 16-17 0.23218876627763763 1103 18-19 0.05637102483530754 1103 20-21 0.14053521964954285 1103 22-23 0.25288152455314616 1103 24-25 0.11702899177020498 1103 26-27 -0.010170318452544791 1103 28-29 0.0806269638283581 1103 30-31 0.19910475951545692 1103 32-33 0.11008886419811148 1103 34-35 0.09554316488804204 1103 36-37 0.1195931886268582 1103 38-39 0.23511462764943758 1103 40-41 0.2919284694420199 1103 42-43 0.2614012560681829 1103 44-45 0.3486103534308427 1103 46-47 0.28098056481410794 1103 48-49 0.024372811324305133 1103 50-51 0.09390106757025762 1103 52-53 0.06935246458328237 1103 54-55 0.2179854733096107 1103 56-57 0.21568198440355957 1103 58-59 0.1983514609665349 1103 60-61 0.07233445527133142 1103 62-63 0.108671518921021 1103 64-65 0.18950312848479456 1103 66-67 0.2957698471549932 1103 68-69 0.15331818146579224 1103 70-71 0.012120066453810807 1103 72-73 -0.07130047509456716 1103 74-75 0.15186487136169546 1103 76-77 0.1833827222029143 1103 78-79 -0.081795584096092 1103 80-81 0.11927232021814405 1103 82-83 0.12765688517247042 1103 84-85 0.22296701624502901 1103 86-87 0.2367284402367531 1103 88-89 0.5039319354753502 1103 90-91 0.39713920672734204 1103 92-93 0.3849850156953458 1103 94-95 0.3814698882427052 1103 96-97 0.3806620682518087 1103 98-99 0.39680158757631645 1103 100-101 0.5730166213308543 1104 1 0.04646289756112054 1104 2 0.043753704338840294 1104 3 0.03677591646282252 1104 4 0.06480693604984822 1104 5 0.09657410602221006 1104 6 -0.014062643889360515 1104 7 0.03873750921022179 1104 8 0.08014784155005827 1104 9 0.01634986639788849 1104 10-11 0.03642577779603329 1104 12-13 0.13808835578762313 1104 14-15 0.16212107731092118 1104 16-17 0.22158312984613104 1104 18-19 0.13758603043441298 1104 20-21 0.06887135253494847 1104 22-23 0.166832625710299 1104 24-25 0.14829065657050222 1104 26-27 0.15549784130855215 1104 28-29 0.25244480630689736 1104 30-31 0.2003132328263888 1104 32-33 0.1651980467289107 1104 34-35 0.12898381438412088 1104 36-37 0.15098083327038836 1104 38-39 0.35444553470430407 1104 40-41 0.41621924958386813 1104 42-43 0.4326451903720283 1104 44-45 0.3921340563050393 1104 46-47 0.4644258802303085 1104 48-49 0.44825765641575543 1104 50-51 0.47800427772329357 1104 52-53 0.49926101254893496 1104 54-55 0.43150264389564796 1104 56-57 0.5601668668223709 1104 58-59 0.48533354383327776 1104 60-61 0.5038807411989126 1104 62-63 0.2794488612129271 1104 64-65 0.1914208216538853 1104 66-67 0.2910059427130349 1104 68-69 0.2817942919547818 1104 70-71 0.4331213729114367 1104 72-73 0.15702166003048035 1104 74-75 0.07212429652034302 1104 76-77 0.13251952698082192 1104 78-79 0.2692869093716226 1104 80-81 0.28575701600015435 1104 82-83 0.2820988411261851 1104 84-85 0.2975657471147528 1104 86-87 0.2950151143688906 1104 88-89 0.40587762416515716 1104 90-91 0.5849424915351094 1104 92-93 0.7081606409286394 1104 94-95 0.535085976335278 1104 96-97 0.4065065997934276 1104 98-99 0.12094772419445832 1104 100-101 0.34275346343611446 1105 1 0.12092478860760991 1105 2 0.1844688085538948 1105 3 0.1448785210650101 1105 4 0.14852352683927705 1105 5 0.12972781161854385 1105 6 0.224616163134705 1105 7 0.163018998262217 1105 8 0.190868210716026 1105 9 0.13322635684355078 1105 10-11 0.12953914457729354 1105 12-13 0.14541050884284346 1105 14-15 0.20255590031566584 1105 16-17 0.34680418035869565 1105 18-19 0.2140455057975359 1105 20-21 0.32944964545203703 1105 22-23 0.23794817589766382 1105 24-25 0.2779594292175318 1105 26-27 0.2900575985014058 1105 28-29 0.30052851902640043 1105 30-31 0.29322296988854646 1105 32-33 0.17942541518256405 1105 34-35 0.1367654892579182 1105 36-37 0.0713379982491702 1105 38-39 0.28784197002283207 1105 40-41 0.25066209389617455 1105 42-43 0.37298671534957606 1105 44-45 0.4486122686993639 1105 46-47 0.3795153843958161 1105 48-49 0.4097286682154433 1105 50-51 0.4160013510299905 1105 52-53 0.410823773860848 1105 54-55 0.3024660524868139 1105 56-57 0.4297362248080674 1105 58-59 0.407516020837825 1105 60-61 0.3841669841918858 1105 62-63 0.4098589854038295 1105 64-65 0.3570201743746253 1105 66-67 0.29408632612534547 1105 68-69 0.3464691812359604 1105 70-71 0.23323015608837494 1105 72-73 0.15848578093852694 1105 74-75 0.19871616991375163 1105 76-77 0.2682348634731184 1105 78-79 0.09745439675121759 1105 80-81 0.199296069547799 1105 82-83 0.3024347140240735 1105 84-85 0.21920159833343433 1105 86-87 0.2897679713790211 1105 88-89 0.3619396731601725 1105 90-91 0.5142636225704429 1105 92-93 0.44759705890380275 1105 94-95 0.271543051500192 1105 96-97 0.253095608434144 1105 98-99 0.3176397090809502 1105 100-101 0.3613488055030736 1106 1 0.016707566141946018 1106 2 0.10091461626305431 1106 3 0.10932677660662904 1106 4 0.050251364133970355 1106 5 0.0449716127094959 1106 6 0.024459773838685805 1106 7 0.11361357978864106 1106 8 0.08593325959712672 1106 9 0.042304491051254445 1106 10-11 0.0821063466443519 1106 12-13 0.13471710741342946 1106 14-15 0.23370872363353357 1106 16-17 0.029724127171228076 1106 18-19 0.20604169805815076 1106 20-21 0.2450183131965602 1106 22-23 0.2352208070153594 1106 24-25 0.22745210838527186 1106 26-27 0.1285278543150028 1106 28-29 0.21810040521729945 1106 30-31 0.25303998685716067 1106 32-33 0.187034472162658 1106 34-35 0.15613018607422902 1106 36-37 0.19597120113217414 1106 38-39 0.25950302077758636 1106 40-41 0.08336445560119188 1106 42-43 0.0645579366970992 1106 44-45 0.14550223711930244 1106 46-47 0.21258227670629282 1106 48-49 0.21351109821801373 1106 50-51 0.1678417583976639 1106 52-53 0.1889466490062759 1106 54-55 0.24829416025188067 1106 56-57 0.34232103011471793 1106 58-59 0.3764474361987311 1106 60-61 0.392364200915722 1106 62-63 0.38605117047205084 1106 64-65 0.2810104434850942 1106 66-67 0.42927467323402624 1106 68-69 0.5262466098030743 1106 70-71 0.30420256508793386 1106 72-73 0.3633334713632692 1106 74-75 0.4007949387446885 1106 76-77 0.37326009730156073 1106 78-79 0.31740158703241406 1106 80-81 0.287184965152278 1106 82-83 0.2330945710827521 1106 84-85 0.25653406535568735 1106 86-87 0.3339056392897497 1106 88-89 0.4417696092445489 1106 90-91 0.6203605386037481 1106 92-93 0.6389733602591754 1106 94-95 0.6354734091557148 1106 96-97 0.6629254689681332 1106 98-99 0.312935564386855 1106 100-101 0.4356023677037051 1107 1 -0.16178542654263595 1107 2 -0.10407977472460317 1107 3 -0.0410998243318943 1107 4 0.016446399763047737 1107 5 0.03889412933902747 1107 6 0.022709919989004845 1107 7 -0.030452717157217535 1107 8 0.03198404090884566 1107 9 0.012203844829834054 1107 10-11 0.11215564275280343 1107 12-13 0.02013683533683519 1107 14-15 0.14762578925108016 1107 16-17 0.13117338535959533 1107 18-19 0.06017419714965655 1107 20-21 0.047600828878096024 1107 22-23 0.09296203404606018 1107 24-25 0.05868364209464971 1107 26-27 0.2201048557844132 1107 28-29 0.23488675714727236 1107 30-31 0.2014900034471978 1107 32-33 0.0333645190887637 1107 34-35 0.232259098557833 1107 36-37 0.3664148661082365 1107 38-39 0.31880845124953083 1107 40-41 0.31453120916805233 1107 42-43 0.005339011415458117 1107 44-45 0.04676584489924096 1107 46-47 0.12353644080545223 1107 48-49 0.2772567334584082 1107 50-51 0.16536826295597962 1107 52-53 0.25897635307162403 1107 54-55 0.34714312771961175 1107 56-57 0.2057925347520282 1107 58-59 0.10662471258874007 1107 60-61 0.31212056437971825 1107 62-63 0.19247348960114863 1107 64-65 0.2344682810923402 1107 66-67 0.086042618123507 1107 68-69 0.30590169504426257 1107 70-71 0.26185570692484106 1107 72-73 0.2794325217811817 1107 74-75 0.1324104132987287 1107 76-77 0.03278791326275865 1107 78-79 0.12352765550976841 1107 80-81 0.26936845095595174 1107 82-83 0.41388014881101753 1107 84-85 0.3497274103810426 1107 86-87 0.386434039852233 1107 88-89 0.4895303493181764 1107 90-91 0.33735189598473525 1107 92-93 0.46058583280661836 1107 94-95 0.48493660297474506 1107 96-97 -0.006486453738098419 1107 98-99 0.04270401487744735 1107 100-101 0.4385297191438795 1108 1 -0.03938816626866526 1108 2 0.06201611568635457 1108 3 -0.04552904807619029 1108 4 0.0885126098087099 1108 5 -0.0426812131267198 1108 6 0.0038628880255373588 1108 7 0.08588289928113824 1108 8 -0.018735137173344185 1108 9 -0.09554729672267825 1108 10-11 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-0.682746107674113 2316 78-79 -0.8832898920376024 2316 80-81 -0.9894893433686462 2316 82-83 -1.0959446251051688 2316 84-85 -0.8338520470178139 2316 86-87 -1.0717052740880746 2316 88-89 -0.9757551724114037 2316 90-91 -1.0354657940711398 2316 92-93 -1.2273772456146297 2316 94-95 -1.1297471172098064 2316 96-97 -1.013721729343608 2316 98-99 -1.0395910846113026 2316 100-101 -0.8247259470536967 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 4.0 4 12.0 5 15.0 6 43.0 7 83.0 8 199.0 9 351.0 10 609.0 11 1013.0 12 1523.0 13 2192.0 14 2925.0 15 3824.0 16 4912.0 17 6431.0 18 6151.0 19 4409.0 20 4073.0 21 4301.0 22 4719.0 23 5309.0 24 6240.0 25 7622.0 26 9628.0 27 11696.0 28 14739.0 29 18574.0 30 22449.0 31 28189.0 32 35713.0 33 45270.0 34 63113.0 35 93082.0 36 158502.0 37 290064.0 38 321258.0 39 61845.0 40 61.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.550856891729666 18.070826257870525 13.24163102679978 24.136685823600025 2 15.836744166930588 19.01589089845346 40.34139764799553 24.805967286620426 3 20.38133988639568 22.977399991942956 28.105466704266203 28.535793417395155 4 12.866857350038272 15.245054989324416 35.91604560286831 35.972042057769 5 14.050920517262217 36.96499214438222 34.073157958345085 14.910929380010474 6 34.58623051202514 34.833420617975264 16.498972726906498 14.081376143093099 7 30.444990532973453 30.92768803126133 19.66152358699593 18.965797848769288 8 27.000684848729 36.077669902912625 18.450711034121582 18.4709342142368 9 26.307376223663535 16.23333199049269 19.205092051726222 38.25419973411755 10-11 26.018131629961346 25.989649888087303 26.60931912925635 21.38289935269501 12-13 26.26438335138785 22.737246993409734 27.478299333238525 23.52007032196389 14-15 22.763928614591304 24.539499657575636 25.264351609394513 27.432220118438543 16-17 23.11082177173191 27.68842231123526 25.908528560200427 23.292227356832402 18-19 22.976473431897837 26.543689320388346 27.758208113443178 22.721629134270636 20-21 23.585474594317606 25.823077719485042 27.85941580768473 22.732031878512622 22-23 23.04821826244735 25.720945447279455 27.83805799147241 23.39277829880079 24-25 22.963118138785376 25.833497834625845 28.089555846510223 23.113828180078556 26-27 22.987962116582274 26.158476573846194 27.77833954477536 23.07522176479617 28-29 22.770020006107384 26.324548533140607 27.864325879587888 23.041105581164125 30-31 22.98711704957513 26.146358599557747 27.836059933778433 23.03046441708869 32-33 22.746245471381226 26.066151573419322 27.934724238186913 23.25287871701254 34-35 23.096567046628785 26.28873226669997 27.745828910289593 22.868871776381653 36-37 23.23044081313331 26.069341364597896 27.714509815296225 22.98570800697257 38-39 22.92225541283369 26.300902995010667 27.773653010621217 23.003188581534424 40-41 23.258830681272038 26.120792834660673 27.75364452804132 22.866731956025966 42-43 23.102015460403866 26.097408279389512 27.60042252635955 23.200153733847067 44-45 23.001583673857457 26.357312435767742 27.61671789132476 23.024385999050036 46-47 23.141394923813692 26.51943518422268 27.258856169886325 23.08031372207731 48-49 23.198409635020262 26.48804607352456 27.257442345643295 23.056101945811875 50-51 23.179055008160233 26.68733147231906 27.10293740247905 23.030676117041658 52-53 23.348720618048326 26.538876193729916 27.05524596557159 23.05715722265017 54-55 23.188715236163453 26.585907742261668 27.109054762043844 23.116322259531035 56-57 23.06194273853973 26.84753374790712 27.030957671897987 23.059565841655157 58-59 23.240842648738976 26.588829248090857 27.160161594155024 23.010166509015143 60-61 23.123673089551097 26.645006385284436 27.17061399444863 23.060706530715834 62-63 23.09793336824719 26.563106796116504 27.32792168553358 23.011038150102728 64-65 22.98982545023269 26.60304753064934 27.264538293950057 23.14258872516791 66-67 23.003393225393072 26.74093652698567 27.029459384224058 23.2262108633972 68-69 23.048342263223624 26.61402731337872 27.28952987149015 23.048100551907506 70-71 23.07736311653656 26.407186255939063 27.17454096895855 23.340909658565824 72-73 23.115513917788462 26.70595889624555 27.02623219612812 23.15229498983787 74-75 22.95423599081497 26.856302622567778 26.93087056359022 23.25859082302703 76-77 23.09600627429199 26.732867497560964 26.891904492229994 23.27922173591705 78-79 23.165495942592177 26.780867715035356 26.87874395784587 23.1748923845266 80-81 23.11012473678295 26.800461030179267 26.786401301713315 23.30301293132447 82-83 23.23272771220239 26.914837046287715 26.845909035974703 23.006526205535188 84-85 23.245300409984164 26.883834328851858 26.64159842661477 23.22926683454921 86-87 23.100383029254786 26.72375265409909 26.96056444378473 23.21529987286139 88-89 23.251338406668957 26.78304621398378 26.708522563564944 23.25709281578232 90-91 23.03860573365604 26.97782198015985 26.767272437771698 23.21629984841241 92-93 23.082073058881043 26.873361104489312 26.819461896815746 23.2251039398139 94-95 23.016484660873797 26.901795026844628 26.86529583881056 23.21642447347101 96-97 23.161022223923197 26.632289045960594 26.87428869547001 23.332400034646195 98-99 22.859263061080693 26.71960538884017 26.81165930302497 23.609472247054175 100-101 22.95455763173235 26.764552205284886 26.727489609380385 23.553400553602383 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 54.0 1 42.5 2 83.5 3 147.0 4 122.5 5 94.0 6 188.5 7 427.5 8 714.5 9 800.5 10 656.0 11 577.5 12 649.5 13 717.5 14 743.5 15 818.0 16 917.0 17 1001.0 18 1114.5 19 1228.5 20 1389.0 21 1630.5 22 2032.5 23 2560.5 24 3187.0 25 3952.0 26 5135.0 27 6833.0 28 8344.5 29 9854.5 30 11741.0 31 13930.5 32 16331.5 33 18244.0 34 20383.0 35 22797.5 36 25320.5 37 27672.5 38 29697.5 39 31855.5 40 34023.0 41 36195.0 42 38032.0 43 39758.5 44 41775.0 45 43723.0 46 45201.5 47 46461.5 48 48056.0 49 48893.0 50 47841.5 51 47023.5 52 47138.0 53 46939.0 54 46834.0 55 44716.0 56 40844.0 57 37058.5 58 34089.5 59 31114.5 60 26982.0 61 22732.0 62 18391.0 63 14068.0 64 10412.5 65 7797.5 66 5564.0 67 4282.5 68 3249.5 69 2319.5 70 1758.0 71 1155.5 72 799.5 73 648.5 74 481.5 75 331.0 76 226.0 77 122.5 78 69.5 79 34.0 80 19.5 81 9.5 82 5.0 83 4.5 84 2.0 85 1.0 86 1.0 87 1.5 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26620472948475205 2 0.020223180115215726 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.22281754824155E-4 12-13 1.2085565805905813E-4 14-15 0.0 16-17 6.848487290013294E-4 18-19 0.0 20-21 4.0285219353019375E-5 22-23 1.611408774120775E-4 24-25 0.002014260967650969 26-27 0.002135116625710027 28-29 0.002336542722475124 30-31 0.0010877009225315232 32-33 0.0012488417999436006 34-35 0.0014099826773556782 36-37 4.0285219353019375E-5 38-39 2.0142609676509688E-4 40-41 6.848487290013294E-4 42-43 0.0035450993030657053 44-45 0.0039076662772428794 46-47 0.014422108528380937 48-49 0.01426096765096886 50-51 0.005680215928775732 52-53 0.006606775973895178 54-55 6.042782902952907E-4 56-57 0.0029005357934173952 58-59 0.00128912701929662 60-61 0.0012085565805905814 62-63 0.0 64-65 0.0011279861418845426 66-67 4.0285219353019375E-5 68-69 0.0 70-71 2.4171131611811625E-4 72-73 0.001812834870885872 74-75 0.0 76-77 0.19695443741691174 78-79 0.10627240865326512 80-81 0.0013294122386496394 82-83 0.0 84-85 3.625669741771744E-4 86-87 0.12552874350400836 88-89 0.5892519034766145 90-91 0.3151512709986706 92-93 0.2944849534705717 94-95 0.002135116625710027 96-97 0.0026185392579462594 98-99 0.0023768279418281434 100-101 5.237078515892519E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1241150.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.1744239114831 #Duplication Level Percentage of deduplicated Percentage of total 1 81.64110219207407 54.841940072510965 2 11.423720210149913 15.347636480855742 3 3.1448321834094664 6.3375687065646735 4 1.3255369399003714 3.561687212447906 5 0.6889557862049022 2.314010401939861 6 0.4062450211110842 1.6373565156027226 7 0.26485919944201347 1.2454234898121703 8 0.18651645325167626 1.0023308237755537 9 0.1384920699061286 0.8372812511027733 >10 0.7241769616208448 8.668355892512496 >50 0.039718830503333274 1.8027474275755775 >100 0.015123970801187367 1.7839422896065893 >500 3.60090812480502E-4 0.13247450336244462 >1k 3.60090812480502E-4 0.4872449323306469 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2698 0.21737904362889257 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2018 0.1625911453087862 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1327 0.10691697216291343 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 2.4171131611811625E-4 14-15 0.0 0.0 0.0 4.0285219353019375E-5 2.819965354711356E-4 16-17 0.0 0.0 0.0 8.057043870603875E-5 3.22281754824155E-4 18-19 0.0 0.0 0.0 8.057043870603875E-5 3.22281754824155E-4 20-21 0.0 0.0 0.0 8.057043870603875E-5 4.431374128832131E-4 22-23 0.0 0.0 0.0 1.2085565805905813E-4 4.834226322362325E-4 24-25 0.0 0.0 0.0 2.4171131611811625E-4 4.834226322362325E-4 26-27 0.0 0.0 0.0 4.0285219353019375E-4 5.639930709422713E-4 28-29 0.0 0.0 0.0 6.848487290013294E-4 5.639930709422713E-4 30-31 0.0 0.0 0.0 0.0015308383354147364 5.639930709422713E-4 32-33 0.0 0.0 0.0 0.003504814083712686 5.639930709422713E-4 34-35 0.0 0.0 0.0 0.006244208999718004 5.639930709422713E-4 36-37 0.0 0.0 0.0 0.01099786488337429 5.639930709422713E-4 38-39 0.0 0.0 0.0 0.01917576441203722 5.639930709422713E-4 40-41 0.0 0.0 0.0 0.031100189340530958 5.639930709422713E-4 42-43 0.0 0.0 0.0 0.042218909881964306 5.639930709422713E-4 44-45 0.0 0.0 0.0 0.05482818353945937 5.639930709422713E-4 46-47 0.0 0.0 0.0 0.0692100068484873 6.042782902952906E-4 48-49 0.0 0.0 0.0 0.08459896064134069 7.251339483543488E-4 50-51 0.0 0.0 0.0 0.10542641904685171 7.251339483543488E-4 52-53 0.0 0.0 0.0 0.12544817306530234 0.0010071304838254844 54-55 0.0 0.0 0.0 0.14663819844499054 0.0010474157031785038 56-57 0.0 0.0 0.0 0.17201788663739273 0.0011279861418845426 58-59 0.0 0.0 0.0 0.20017725496515326 0.0011279861418845426 60-61 0.0 0.0 0.0 0.23313056439592314 0.0011279861418845426 62-63 0.0 0.0 0.0 0.26749385650404867 0.0012085565805905814 64-65 0.0 0.0 0.0 0.30612738186359423 0.0012085565805905814 66-67 0.0 0.0 0.0 0.3479837247713814 0.0012085565805905814 68-69 0.0 0.0 0.0 0.3962454175562986 0.0012085565805905814 70-71 0.0 0.0 0.0 0.4495427627603432 0.0012085565805905814 72-73 0.0 0.0 0.0 0.5059017846352174 0.0013294122386496394 74-75 0.0 0.0 0.0 0.5646779196712726 0.0013696974580026588 76-77 0.0 0.0 0.0 0.6296982637070458 0.0013696974580026588 78-79 0.0 0.0 0.0 0.7084558675421988 0.0013696974580026588 80-81 0.0 0.0 0.0 0.7939008177899529 0.0014502678967086976 82-83 0.0 0.0 0.0 0.8885307980501953 0.0015308383354147364 84-85 0.0 0.0 0.0 0.9834427748459089 0.0015308383354147364 86-87 0.0 0.0 0.0 1.0848809571768119 0.0015308383354147364 88-89 0.0 0.0 0.0 1.2047294847520447 0.0015308383354147364 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAACGC 75 1.3775752E-7 44.273075 3 GGTATCA 1945 0.0 43.03802 1 CTATACG 50 0.001626698 37.948353 4 GTATCAA 3140 0.0 37.41334 1 TACCTGG 1115 0.0 35.319225 2 TAACGCA 100 1.3131848E-6 33.204807 4 GTACCTG 1245 0.0 32.08996 1 TAGCGCG 60 0.0039774636 31.623629 4 ATCAACG 3755 0.0 30.823563 3 TCAACGC 3825 0.0 30.38349 4 CAACGCA 3910 0.0 29.601658 5 TATCAAC 3950 0.0 29.549215 2 AACGCAG 4100 0.0 28.229874 6 GTGTAGC 825 0.0 27.09587 1 TATACCG 90 9.019281E-4 26.353024 5 TACATGG 4155 0.0 25.12231 2 GTACATG 4290 0.0 24.834187 1 ACGCAGA 4830 0.0 23.963247 7 ACATGGG 4220 0.0 23.830128 3 CGTAAAG 120 1.7267717E-4 23.71772 3 >>END_MODULE