FastQCFastQC Report
Thu 26 May 2016
SRR936312_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936312_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2048039
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34740.16962567607355133No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24440.11933366503274595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54900.037.774481
GGTATCA33450.036.6606671
ATCAACG65500.031.2177283
TACCTGG20450.031.0156522
CAACGCA66100.031.0052095
TCAACGC66050.030.9570224
TATCAAC68450.030.0113622
AACGCAG69100.029.7946476
GTACCTG22500.029.6710781
TACATGG89650.028.4582312
GTACATG94500.027.9074481
ACATGGG90200.026.5523413
ACGCAGA81800.024.7071597
CGCAGAG83400.024.177598
CATGGGG54550.023.6005634
GTGTAGG11750.021.356731
GTGTAGC11950.020.9992941
GCAGAGT97450.020.2545999
TAGCCCG1200.00486383219.7213655
GACCGTA1250.00615797318.9325127
Produced by FastQC (version 0.11.5)