##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936312_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2048039 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.804095039205798 33.0 31.0 34.0 30.0 34.0 2 31.89907076964843 34.0 31.0 34.0 30.0 34.0 3 31.9718105954037 34.0 31.0 34.0 30.0 34.0 4 35.44847974086431 37.0 35.0 37.0 33.0 37.0 5 35.43670262138563 37.0 35.0 37.0 33.0 37.0 6 35.51965856118951 37.0 35.0 37.0 33.0 37.0 7 35.46140869387741 37.0 35.0 37.0 33.0 37.0 8 35.46217235121011 37.0 35.0 37.0 33.0 37.0 9 37.14113549595491 39.0 37.0 39.0 34.0 39.0 10-11 37.06021931222989 39.0 37.0 39.0 33.5 39.0 12-13 36.9697803606279 39.0 37.0 39.0 33.0 39.0 14-15 38.24360253881884 40.0 38.0 41.0 33.0 41.0 16-17 38.19219604704793 40.0 38.0 41.0 33.0 41.0 18-19 38.129311258232875 40.0 38.0 41.0 33.0 41.0 20-21 38.04110224463499 40.0 38.0 41.0 33.0 41.0 22-23 37.887863463537556 40.0 38.0 41.0 33.0 41.0 24-25 38.03873485807643 40.0 38.0 41.0 33.0 41.0 26-27 37.9980874387646 40.0 38.0 41.0 33.0 41.0 28-29 37.87257127427749 40.0 38.0 41.0 33.0 41.0 30-31 37.730083997423876 40.0 38.0 41.0 32.5 41.0 32-33 37.56974525387456 40.0 38.0 41.0 32.0 41.0 34-35 37.31487828112648 40.0 37.0 41.0 31.5 41.0 36-37 37.12571928561907 40.0 37.0 41.0 31.0 41.0 38-39 36.96712171985006 40.0 37.0 41.0 31.0 41.0 40-41 36.78897008308924 40.0 36.5 41.0 30.0 41.0 42-43 36.60494502301958 40.0 36.0 41.0 30.0 41.0 44-45 36.42620794818849 40.0 36.0 41.0 30.0 41.0 46-47 36.169881774712294 39.5 35.5 41.0 29.5 41.0 48-49 36.004787018215964 39.0 35.0 41.0 28.5 41.0 50-51 35.694506794060075 39.0 35.0 40.5 28.5 41.0 52-53 35.70006552609594 39.0 35.0 40.5 28.0 41.0 54-55 35.76005730359627 39.0 35.0 41.0 28.0 41.0 56-57 35.58814920028378 39.0 35.0 41.0 28.0 41.0 58-59 35.34664403363412 38.5 35.0 41.0 28.0 41.0 60-61 35.04149579182818 38.0 35.0 40.5 27.5 41.0 62-63 34.727421206334455 37.5 34.5 40.0 26.5 41.0 64-65 34.39110949547347 37.0 34.0 40.0 26.0 41.0 66-67 34.030368318181445 36.5 34.0 39.5 26.0 41.0 68-69 33.65187137549627 36.0 34.0 39.0 26.0 41.0 70-71 33.249684210115134 35.0 33.5 39.0 25.0 40.5 72-73 32.85610747646895 35.0 33.0 37.5 24.5 40.0 74-75 32.45514611782295 35.0 33.0 37.0 23.5 39.0 76-77 32.06924404271598 35.0 33.0 36.5 22.5 39.0 78-79 31.693800264545743 35.0 32.0 36.0 21.0 38.5 80-81 31.331167765848207 35.0 32.0 36.0 20.0 37.0 82-83 31.009695127875982 35.0 32.0 35.0 18.5 37.0 84-85 30.71574540328578 35.0 31.5 35.0 18.0 36.5 86-87 30.415162015957705 34.5 31.0 35.0 14.0 36.0 88-89 30.11743916985956 34.0 31.0 35.0 8.5 36.0 90-91 29.79528929869011 34.0 31.0 35.0 4.5 35.5 92-93 29.50970904362661 34.0 30.5 35.0 2.0 35.0 94-95 29.161179059578455 34.0 30.0 35.0 2.0 35.0 96-97 28.76879712739845 34.0 30.0 35.0 2.0 35.0 98-99 28.309413297305376 34.0 29.5 35.0 2.0 35.0 100-101 25.09690147502074 30.5 23.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32394109231536916 1101 2 0.2637209624681667 1101 3 0.28755775952186724 1101 4 0.0773888146194679 1101 5 0.33244803200913253 1101 6 0.33676965634294476 1101 7 0.3673832485966244 1101 8 0.33223405437269093 1101 9 0.4541723682950902 1101 10-11 0.5483310820607912 1101 12-13 0.40551791632442047 1101 14-15 0.48986840402245946 1101 16-17 0.5627255651198837 1101 18-19 0.5928720114391197 1101 20-21 0.5800519571724223 1101 22-23 0.5003338727833508 1101 24-25 0.47093570147864483 1101 26-27 0.5684660492182161 1101 28-29 0.42681303685687766 1101 30-31 0.6302197432574346 1101 32-33 0.6201702923177805 1101 34-35 0.47018094076847916 1101 36-37 0.5309852310728544 1101 38-39 0.5646875602445292 1101 40-41 0.46232719050811966 1101 42-43 0.38633835139133765 1101 44-45 0.4205037206367237 1101 46-47 0.3643694912022184 1101 48-49 0.3901531827902005 1101 50-51 0.5062584035694044 1101 52-53 0.5044765769739996 1101 54-55 0.47797655450031584 1101 56-57 0.5235493758005276 1101 58-59 0.5644592407471265 1101 60-61 0.4267798453630576 1101 62-63 0.34244884881317716 1101 64-65 0.42805062817060247 1101 66-67 0.6207941554198086 1101 68-69 0.6082492222525104 1101 70-71 0.5871782644618335 1101 72-73 0.7579763858135635 1101 74-75 0.608675514732461 1101 76-77 0.5457430800558569 1101 78-79 0.5013514828986558 1101 80-81 0.5193918879601931 1101 82-83 0.5589973135174695 1101 84-85 0.5463679536458805 1101 86-87 0.4990024132629287 1101 88-89 0.3838080511061115 1101 90-91 0.5057583671282764 1101 92-93 0.4682122343128974 1101 94-95 0.5865710790742185 1101 96-97 0.6918421417827929 1101 98-99 0.7110720481814177 1101 100-101 0.7727024494129147 1102 1 0.21357158927485997 1102 2 0.16999300909713 1102 3 0.18181981845077644 1102 4 0.03168665295712003 1102 5 0.15082166950438136 1102 6 0.08108570450355757 1102 7 0.03062126046317104 1102 8 0.046259804219708656 1102 9 0.08214654703699864 1102 10-11 0.21411326721492685 1102 12-13 0.21655360462248296 1102 14-15 0.28712621891757806 1102 16-17 0.3098591185963002 1102 18-19 0.37740618963929506 1102 20-21 0.24838508735356868 1102 22-23 0.3995100599000523 1102 24-25 0.435943199641045 1102 26-27 0.3777400079401545 1102 28-29 0.28471408696898237 1102 30-31 0.2571830859850195 1102 32-33 0.08738460802119619 1102 34-35 0.01984749416241982 1102 36-37 0.16007474608087335 1102 38-39 0.25428256713323805 1102 40-41 0.18058773995474553 1102 42-43 0.1497468571885605 1102 44-45 0.1788389623693547 1102 46-47 0.2993481725352396 1102 48-49 0.25615754255917267 1102 50-51 0.1684346374726715 1102 52-53 0.22339321563028136 1102 54-55 0.29907966469868086 1102 56-57 0.3837654373814914 1102 58-59 0.3258063674980747 1102 60-61 0.31743410620306634 1102 62-63 0.5453951095202214 1102 64-65 0.31526930856789903 1102 66-67 0.3179048696659521 1102 68-69 0.2877169389081544 1102 70-71 0.29873910115333047 1102 72-73 0.2992646045875347 1102 74-75 0.19153374898293407 1102 76-77 0.06614002095334826 1102 78-79 0.2831785043552131 1102 80-81 0.23779341270966725 1102 82-83 0.24710176821391983 1102 84-85 0.25916801865458083 1102 86-87 0.37400733816433984 1102 88-89 0.23268560451358766 1102 90-91 0.29781579394116164 1102 92-93 0.3313544568470306 1102 94-95 0.38430322353635304 1102 96-97 0.32836056276090275 1102 98-99 0.43281388731197623 1102 100-101 0.3823532246539578 1103 1 0.20891821360875085 1103 2 0.13645060718383561 1103 3 0.23899788592249394 1103 4 0.12036913458313592 1103 5 0.13763596596771066 1103 6 0.18048054873329988 1103 7 0.18490804795495563 1103 8 0.10862912397517732 1103 9 0.09636461707626154 1103 10-11 0.14798995498854595 1103 12-13 0.24453106372900635 1103 14-15 0.3608748979995795 1103 16-17 0.4249465874380931 1103 18-19 0.27777762167486486 1103 20-21 0.3930988597289442 1103 22-23 0.37915826481856385 1103 24-25 0.35944005737178486 1103 26-27 0.395286078112548 1103 28-29 0.32557294814451154 1103 30-31 0.3534604746029615 1103 32-33 0.3468947718090831 1103 34-35 0.28036981693355756 1103 36-37 0.18183913591482792 1103 38-39 0.19939047528649922 1103 40-41 0.2689217904569077 1103 42-43 0.1927312225119806 1103 44-45 0.3534892831340031 1103 46-47 0.2162407374756654 1103 48-49 0.3554733397279861 1103 50-51 0.38030230556060474 1103 52-53 0.39213536292884044 1103 54-55 0.26853733648339073 1103 56-57 0.39449607590344726 1103 58-59 0.43347381354102765 1103 60-61 0.5174907764080388 1103 62-63 0.4605544339234129 1103 64-65 0.31838141513046736 1103 66-67 0.2860136895349328 1103 68-69 0.3678277177178373 1103 70-71 0.5099735012455753 1103 72-73 0.4988031599400955 1103 74-75 0.47119913029288085 1103 76-77 0.45211493272375236 1103 78-79 0.4112457383380814 1103 80-81 0.3474989950963199 1103 82-83 0.4446901493657691 1103 84-85 0.4758401730495372 1103 86-87 0.5440622312962802 1103 88-89 0.5023243416149477 1103 90-91 0.5290840178547391 1103 92-93 0.6346880437649212 1103 94-95 0.6186924690945581 1103 96-97 0.7115590208083091 1103 98-99 0.6064677990716802 1103 100-101 0.7839497375493281 1104 1 0.2632172762182883 1104 2 0.13653939493887535 1104 3 0.23499433903966604 1104 4 0.26233930883850576 1104 5 0.2581597038222938 1104 6 0.15376092947370523 1104 7 0.2549988948918056 1104 8 0.19868767183553615 1104 9 0.2078117591517099 1104 10-11 0.19608558778784158 1104 12-13 0.30788748959093937 1104 14-15 0.4277510208610096 1104 16-17 0.4421890537826201 1104 18-19 0.32991815851976725 1104 20-21 0.40322471275657534 1104 22-23 0.454149646332894 1104 24-25 0.4118527417003719 1104 26-27 0.354857860843417 1104 28-29 0.335613628553709 1104 30-31 0.20913482194065836 1104 32-33 0.40203211229479763 1104 34-35 0.266872776718202 1104 36-37 0.3383268311466665 1104 38-39 0.27795679308766097 1104 40-41 0.1809662398629186 1104 42-43 0.3141489113464715 1104 44-45 0.1799502899813632 1104 46-47 0.17197425736932814 1104 48-49 0.210884649726232 1104 50-51 0.1956968008354778 1104 52-53 0.1587061368919862 1104 54-55 0.37711593996480275 1104 56-57 0.2739048072970647 1104 58-59 0.12383996614491366 1104 60-61 0.2029685412934299 1104 62-63 0.21753916705691978 1104 64-65 0.21553988883714936 1104 66-67 0.2186528491573867 1104 68-69 0.20994906252551715 1104 70-71 0.21132316188546696 1104 72-73 0.29436400355680803 1104 74-75 0.2161265152129559 1104 76-77 0.1823505470829545 1104 78-79 0.12737508966110767 1104 80-81 0.13206591908359044 1104 82-83 0.3484359803902386 1104 84-85 0.22904087039492893 1104 86-87 0.125894752718132 1104 88-89 0.20232439945713665 1104 90-91 0.2017172023794096 1104 92-93 0.12753151933990026 1104 94-95 0.0022455930931677415 1104 96-97 0.20538523511823215 1104 98-99 0.35618755368199473 1104 100-101 0.7313139269894258 1105 1 0.06515952803430736 1105 2 0.08509264024907637 1105 3 0.09942666737303796 1105 4 0.0394244659309777 1105 5 -0.03967533076772156 1105 6 -0.015684407351741925 1105 7 0.09802529128437953 1105 8 0.1501675784400618 1105 9 0.06279849981109464 1105 10-11 0.2021837727561433 1105 12-13 0.1390577293764821 1105 14-15 0.06744429581124223 1105 16-17 0.013741176260559484 1105 18-19 0.06171774308037925 1105 20-21 0.09385933960369641 1105 22-23 0.08108271224648433 1105 24-25 0.002887671504112177 1105 26-27 0.027184392665688506 1105 28-29 0.0651912081674837 1105 30-31 0.12726527059181336 1105 32-33 0.09585408337709111 1105 34-35 -0.16958130246384684 1105 36-37 -0.15802645823305994 1105 38-39 0.08338726485680326 1105 40-41 -0.02223490104183412 1105 42-43 0.008024615325815887 1105 44-45 0.15498202374669034 1105 46-47 0.033450991309955214 1105 48-49 0.15078746872219284 1105 50-51 0.09883064021772725 1105 52-53 0.057562581119661616 1105 54-55 0.21674875471629207 1105 56-57 0.2930277324874737 1105 58-59 0.21465768391921358 1105 60-61 0.1731142871445286 1105 62-63 0.020066352054584513 1105 64-65 0.06813701211751066 1105 66-67 -0.03776110566079893 1105 68-69 0.09174096484966299 1105 70-71 0.16843846641132387 1105 72-73 0.10676578423935723 1105 74-75 0.15214802319464837 1105 76-77 0.25330163076289836 1105 78-79 0.1802847474153353 1105 80-81 0.2018447134314023 1105 82-83 0.16375786609851062 1105 84-85 0.007615725736563661 1105 86-87 0.16812097078019406 1105 88-89 0.12410198631130598 1105 90-91 0.13938262345006436 1105 92-93 0.03088271327917269 1105 94-95 0.02064490576817235 1105 96-97 0.0843915784506386 1105 98-99 0.07847855337897869 1105 100-101 0.19710146454900368 1106 1 0.18155206605845464 1106 2 0.1771508459015827 1106 3 0.24419852250314733 1106 4 0.2447831393574873 1106 5 0.29250808146162655 1106 6 0.17004176278354777 1106 7 0.20467160371259752 1106 8 0.14107427573145515 1106 9 0.17711714634760511 1106 10-11 0.0864860331970263 1106 12-13 0.08669771144982263 1106 14-15 0.23884579716975196 1106 16-17 0.16184837213742043 1106 18-19 0.1555188971096797 1106 20-21 0.05535985779646779 1106 22-23 0.14817326993394175 1106 24-25 0.24666865803109772 1106 26-27 0.33674168797955417 1106 28-29 0.29398718026910586 1106 30-31 0.1441135381473444 1106 32-33 0.13526978601846906 1106 34-35 0.1642978605848029 1106 36-37 0.25758654980592866 1106 38-39 0.35433079585032345 1106 40-41 0.33208934757865194 1106 42-43 0.21378271733976106 1106 44-45 0.2629675143491923 1106 46-47 0.3537324960297141 1106 48-49 0.3390887768088007 1106 50-51 0.37245644901859976 1106 52-53 0.4177145376474911 1106 54-55 0.43084891997776964 1106 56-57 0.3397274047655614 1106 58-59 0.37490725676030934 1106 60-61 0.48460570195082653 1106 62-63 0.41325421794069683 1106 64-65 0.3713727758216123 1106 66-67 0.3657817922972413 1106 68-69 0.36754864530679043 1106 70-71 0.43647003415451735 1106 72-73 0.4994340568367619 1106 74-75 0.3914065873805228 1106 76-77 0.6033358034751402 1106 78-79 0.6233737365192837 1106 80-81 0.5738761691329195 1106 82-83 0.4214475666671227 1106 84-85 0.5421150394812777 1106 86-87 0.5113255028661321 1106 88-89 0.4611034708635593 1106 90-91 0.5742312271306282 1106 92-93 0.46586912822553117 1106 94-95 0.47642919752470547 1106 96-97 0.36698814437315974 1106 98-99 0.2781655369368643 1106 100-101 0.5314932182813941 1107 1 0.10626436039785148 1107 2 0.25518264110307953 1107 3 0.3155185493887984 1107 4 0.18999807842642724 1107 5 0.23121698587145545 1107 6 0.29764367984716245 1107 7 0.20016475079462737 1107 8 0.30581199454264407 1107 9 0.16596807711705708 1107 10-11 0.3160069134097725 1107 12-13 0.3173384373623236 1107 14-15 0.33715682320791984 1107 16-17 0.10059804337270606 1107 18-19 0.3125484713228346 1107 20-21 0.41676521739200467 1107 22-23 0.47897487138342854 1107 24-25 0.45555041962553844 1107 26-27 0.4808109185239857 1107 28-29 0.5436538859004756 1107 30-31 0.39657562061615437 1107 32-33 0.4224079256484998 1107 34-35 0.2909064698369761 1107 36-37 0.19330354913378756 1107 38-39 0.147628525181986 1107 40-41 0.363493830468272 1107 42-43 -0.028498763708775243 1107 44-45 0.2754719709376374 1107 46-47 0.4342278348765589 1107 48-49 0.4048459584914994 1107 50-51 0.2813788313208292 1107 52-53 0.35569007755222515 1107 54-55 0.3983900520973549 1107 56-57 0.3901591824329387 1107 58-59 0.463897876006925 1107 60-61 0.5412688130879246 1107 62-63 0.6241370608057721 1107 64-65 0.28513179858673965 1107 66-67 0.3585251512498715 1107 68-69 0.49458070352006445 1107 70-71 0.4128455857486628 1107 72-73 0.25979087614767593 1107 74-75 0.2793889948149868 1107 76-77 0.3843250199922821 1107 78-79 0.36818860545174914 1107 80-81 0.44806872297727907 1107 82-83 0.4070456262246793 1107 84-85 0.40439798170635655 1107 86-87 0.198000887886149 1107 88-89 0.29818199449078264 1107 90-91 0.45265929340959943 1107 92-93 0.08162207249625908 1107 94-95 0.2527188319383349 1107 96-97 0.6073335665070623 1107 98-99 0.6079830359870968 1107 100-101 0.5707468493050953 1108 1 -0.09737914470625597 1108 2 -0.09027717306380012 1108 3 -0.14563804677611358 1108 4 -0.06759018196796518 1108 5 -0.026349757650500294 1108 6 -0.13555858685754174 1108 7 -0.21582848899526397 1108 8 -0.08600913485842909 1108 9 -0.12827235912215684 1108 10-11 -0.14534123880784477 1108 12-13 -0.20711562788745397 1108 14-15 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2644.0 6 2524.0 7 2608.0 8 3074.0 9 3798.0 10 4418.0 11 5300.0 12 6676.0 13 9532.0 14 10316.0 15 8461.0 16 9437.0 17 11597.0 18 11002.0 19 8617.0 20 8535.0 21 8920.0 22 9514.0 23 10336.0 24 11888.0 25 14118.0 26 16734.0 27 19998.0 28 24256.0 29 29761.0 30 36399.0 31 45266.0 32 57740.0 33 76434.0 34 108690.0 35 164352.0 36 281797.0 37 467143.0 38 450945.0 39 82448.0 40 116.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.89984147958897 17.335928932051168 12.461169826897665 24.303059761462197 2 16.0898856432409 18.613518299132437 39.64479964371556 25.65179641391111 3 20.365701505729195 22.839179723074746 27.545834767768067 29.249284003427995 4 13.047588447674583 14.389672714062074 34.837206667793005 37.72553217047034 5 14.646232576536281 36.85319244000518 33.18042761190203 15.320147371556509 6 35.600093753204455 33.50344005898639 16.38322598916955 14.513240198639602 7 31.54844944112105 30.84300850757762 19.00198199262771 18.606560058673622 8 28.19188105390429 34.687272322413925 18.33384443797696 18.787002185704825 9 27.46789415546173 15.687547063666704 18.635893499134177 38.20866528173739 10-11 26.875227051668173 25.454786932761458 25.847195692170672 21.8227903233997 12-13 26.90266169499206 22.14014770483254 26.912794184000138 24.04439641617526 14-15 23.568769875907197 23.80088995232834 24.469611607224607 28.160728564539856 16-17 24.11563400796272 27.0427693949335 25.01219069990611 23.829405897197663 18-19 23.955605321384326 26.00225215876645 26.704977456437675 23.337165063411554 20-21 24.662910101222185 25.222268880891807 26.668725616097895 23.44609540178811 22-23 24.135013741334753 25.022120912026157 26.638925187368518 24.20394015927057 24-25 24.02118872425429 25.16682347637977 27.01088616738096 23.80110163198498 26-27 23.907553238605537 25.48936643971907 26.703584105301864 23.899496216373528 28-29 23.637683533971014 25.70409578256754 26.850344499712968 23.807876183748483 30-31 23.852111575575265 25.511241551744344 26.71445872563191 23.92218814704848 32-33 23.689602104127655 25.400573156013245 26.859808734757003 24.050016005102098 34-35 24.06928988297798 25.56818320900692 26.629718785845736 23.732808122169367 36-37 24.29800881835297 25.340659475436777 26.539857360994425 23.82147434521583 38-39 23.915109218806034 25.554997937138907 26.564647025089393 23.965245818965666 40-41 24.03346451332273 25.452033682369745 26.85320868413002 23.661293120177504 42-43 24.100752691627516 25.363963677103314 26.547938800476985 23.987344830792185 44-45 23.846009467998584 25.594951709281656 26.605640316646774 23.95339850607299 46-47 24.136673772313102 25.74685349413293 26.27555677254472 23.840915961009237 48-49 24.306082096748785 25.743459806278647 26.155818241333602 23.79463985563897 50-51 24.10600544555245 25.790175673669317 26.16829677401939 23.935522106758842 52-53 24.257221301099126 25.750692151008785 26.160105944560684 23.831980603331402 54-55 24.187123070623326 25.660798706924155 26.104900974345803 24.047177248106717 56-57 23.944693419573838 26.095995552549372 26.0196224622753 23.939688565601493 58-59 24.137075762854074 25.726030018596024 26.15665801469037 23.980236203859533 60-61 24.027565606432795 25.756266990232906 26.302285023032262 23.913882380302038 62-63 23.932769802589245 25.833011760844272 26.343741651483455 23.890476785083024 64-65 23.932590886005137 25.880153396586113 26.185572575085075 24.001683142323667 66-67 23.897171459639463 26.05989010409474 25.993342273424012 24.049596162841784 68-69 23.897854165076946 25.759784359011036 26.303744936713365 24.038616539198653 70-71 23.967500895393506 25.651110533262028 26.169482045520333 24.21190652582413 72-73 23.94101826030658 25.943295431991636 25.93085709925814 24.184829208443638 74-75 23.824674625220617 26.190869361562946 25.8590598378469 24.12539617536954 76-77 24.077046233922736 25.966402181694352 25.874844029755913 24.081707554627 78-79 24.028961983777638 26.08651599003165 25.937697339271686 23.946824686919022 80-81 23.898379226583206 26.18188295092979 25.896334583491676 24.02340323899532 82-83 24.218139334275765 26.172588593374552 25.808504551786537 23.80076752056314 84-85 24.02567229065822 26.212282169485206 25.55244247409832 24.209603065758255 86-87 23.993598442349793 25.935631616511074 26.000795410466885 24.06997453067225 88-89 24.117810018842594 25.99554245890098 25.803067767826054 24.083579754430364 90-91 23.883919915452456 26.322049364887146 25.834418566294765 23.95961215336563 92-93 23.967874683091704 26.114470552406345 25.813163801863944 24.10449096263801 94-95 24.049178883982908 26.03145209111828 25.96015630117638 23.959212723722434 96-97 24.117058258101814 25.922443041970467 25.859947349586626 24.10055135034109 98-99 23.794571580627995 25.993481187905125 25.80937011794194 24.40257711352494 100-101 23.95346624068032 26.04996755067172 25.689027025409565 24.30753918323839 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 66.0 1 57.0 2 106.5 3 178.5 4 150.0 5 157.5 6 297.0 7 559.5 8 906.5 9 1059.0 10 1000.0 11 1010.0 12 1153.0 13 1329.5 14 1551.5 15 1803.0 16 2031.5 17 2133.5 18 2134.5 19 2249.0 20 2344.5 21 2442.0 22 2665.0 23 3127.5 24 3636.0 25 4435.5 26 5927.0 27 7715.5 28 9342.0 29 11247.0 30 13512.5 31 16539.5 32 20380.0 33 23452.0 34 26079.5 35 28953.5 36 33343.5 37 37936.5 38 41501.5 39 44754.5 40 48156.0 41 52608.0 42 55439.0 43 59007.0 44 64199.0 45 68998.0 46 72821.0 47 75138.5 48 79724.5 49 82383.0 50 80087.0 51 79868.0 52 82466.5 53 84372.0 54 85965.0 55 82987.5 56 76415.5 57 69573.5 58 64776.0 59 60623.0 60 53852.0 61 46387.0 62 38628.5 63 30062.5 64 22736.5 65 17555.5 66 12707.5 67 10276.0 68 7946.0 69 5662.5 70 4480.5 71 3130.0 72 2430.5 73 2059.0 74 1544.0 75 1165.0 76 847.0 77 522.0 78 336.0 79 232.5 80 149.0 81 83.5 82 50.5 83 44.0 84 39.0 85 26.5 86 24.5 87 20.0 88 18.5 89 19.5 90 21.0 91 21.0 92 19.0 93 12.5 94 10.0 95 12.5 96 13.5 97 18.0 98 14.5 99 10.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04804596006228397 2 0.01157204525890376 3 0.0094724758659381 4 0.0051268554944510335 5 0.007519388058528182 6 0.0053221642751920244 7 0.004882719518524793 8 0.009130685499641364 9 0.01372044184705467 10-11 0.002294878173706653 12-13 0.008349450376677397 14-15 0.00861799995019626 16-17 0.018139303011319607 18-19 0.011034946111866034 20-21 0.0033690764677821077 22-23 0.009911920622605331 24-25 0.021508379479101714 26-27 0.00664049854519372 28-29 0.017553376669096635 30-31 0.013622787456684174 32-33 0.012426521174645601 34-35 0.2652293242462668 36-37 0.47247635421005163 38-39 0.46913169133986216 40-41 0.48824753825488676 42-43 0.47950747031672736 44-45 0.494204456067487 46-47 0.49916041637878966 48-49 0.5063380140710211 50-51 0.5173973737804798 52-53 0.5063136004734285 54-55 0.4935208753348935 56-57 0.48912642776822124 58-59 0.4867583088017367 60-61 0.4843413626400669 62-63 0.4822906204422865 64-65 0.497256155766565 66-67 0.47145098311116146 68-69 0.4984035948534183 70-71 0.479922501475802 72-73 0.487515130327108 74-75 0.5016261897356447 76-77 0.48778367990062693 78-79 0.4876860255102564 80-81 0.48976118130562946 82-83 0.4906156572213713 84-85 0.49042034844063026 86-87 0.49303260338304106 88-89 0.5062647732782432 90-91 0.5293844501984581 92-93 0.5166161386575158 94-95 0.49073772520933434 96-97 0.46378511346707757 98-99 0.4552647679072518 100-101 0.4626865015754094 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2048039.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.688044660806305 #Duplication Level Percentage of deduplicated Percentage of total 1 71.42826607066264 34.06274342421716 2 14.188551629266522 13.532485675372358 3 5.605594432583454 8.019595129542207 4 2.7005585271263852 5.151374226028974 5 1.5477936335785176 3.6905625961902007 6 0.9840173664101894 2.815551846982687 7 0.6676812214207366 2.228828833440565 8 0.4701400944482184 1.7936049456705867 9 0.3437465167013784 1.4753339316406742 >10 1.8670901175074153 16.055735356636095 >50 0.12795435334147484 4.213865533706266 >100 0.06490997956468235 5.431429402475684 >500 0.003080047823625659 0.9646194962915966 >1k 6.160095647251311E-4 0.5642696018049255 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3474 0.16962567607355133 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2444 0.11933366503274595 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 4.8827195185247934E-5 0.0 7 0.0 0.0 0.0 4.8827195185247934E-5 0.0 8 0.0 0.0 0.0 4.8827195185247934E-5 0.0 9 0.0 0.0 0.0 4.8827195185247934E-5 0.0 10-11 0.0 0.0 0.0 4.8827195185247934E-5 0.0 12-13 0.0 0.0 0.0 4.8827195185247934E-5 0.0 14-15 0.0 0.0 0.0 4.8827195185247934E-5 9.765439037049587E-5 16-17 0.0 0.0 0.0 9.765439037049587E-5 9.765439037049587E-5 18-19 0.0 0.0 0.0 9.765439037049587E-5 9.765439037049587E-5 20-21 0.0 0.0 0.0 9.765439037049587E-5 9.765439037049587E-5 22-23 0.0 0.0 0.0 1.9530878074099176E-4 1.4648158555574382E-4 24-25 0.0 0.0 0.0 3.417903662967356E-4 1.9530878074099174E-4 26-27 0.0 0.0 0.0 4.8827195185247935E-4 1.9530878074099174E-4 28-29 0.0 0.0 0.0 9.521303061123347E-4 1.9530878074099174E-4 30-31 0.0 0.0 0.0 0.002343705368891901 1.9530878074099174E-4 32-33 0.0 0.0 0.0 0.005175682689636281 1.9530878074099174E-4 34-35 0.0 0.0 0.0 0.009570130256308595 1.9530878074099174E-4 36-37 0.0 0.0 0.0 0.016259455996687565 1.9530878074099174E-4 38-39 0.0 0.0 0.0 0.02836860040262905 1.9530878074099174E-4 40-41 0.0 0.0 0.0 0.04308999975098131 1.9530878074099174E-4 42-43 0.0 0.0 0.0 0.058006707880074546 1.9530878074099174E-4 44-45 0.0 0.0 0.0 0.07582863412269004 1.9530878074099174E-4 46-47 0.0 0.0 0.0 0.09575012975827121 2.441359759262397E-4 48-49 0.0 0.0 0.0 0.12101820326663701 2.9296317111148764E-4 50-51 0.0 0.0 0.0 0.14919149488852507 2.9296317111148764E-4 52-53 0.0 0.0 0.0 0.17738920010800574 4.1503115907460744E-4 54-55 0.0 0.0 0.0 0.20729585715897011 4.394447566672314E-4 56-57 0.0 0.0 0.0 0.2447219022684627 4.882719518524794E-4 58-59 0.0 0.0 0.0 0.28673770372536855 5.370991470377273E-4 60-61 0.0 0.0 0.0 0.3341977374454295 5.370991470377273E-4 62-63 0.0 0.0 0.0 0.3796558561628953 5.370991470377273E-4 64-65 0.0 0.0 0.0 0.42945959525184824 5.370991470377273E-4 66-67 0.0 0.0 0.0 0.4882231246572941 5.859263422229753E-4 68-69 0.0 0.0 0.0 0.558290149748125 5.859263422229753E-4 70-71 0.0 0.0 0.0 0.6360230444830397 5.859263422229753E-4 72-73 0.0 0.0 0.0 0.7158310950133273 6.103399398155993E-4 74-75 0.0 0.0 0.0 0.7969818934112094 6.591671350008472E-4 76-77 0.0 0.0 0.0 0.8876051676750296 7.079943301860951E-4 78-79 0.0 0.0 0.0 0.9913385438460889 7.324079277787191E-4 80-81 0.0 0.0 0.0 1.1100862825366118 7.56821525371343E-4 82-83 0.0 0.0 0.0 1.2392342138015926 7.812351229639669E-4 84-85 0.0 0.0 0.0 1.364451555854161 7.812351229639669E-4 86-87 0.0 0.0 0.0 1.5004597080426691 8.056487205565909E-4 88-89 0.0 0.0 0.0 1.655901083914906 8.300623181492149E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5490 0.0 37.77448 1 GGTATCA 3345 0.0 36.660667 1 ATCAACG 6550 0.0 31.217728 3 TACCTGG 2045 0.0 31.015652 2 CAACGCA 6610 0.0 31.005209 5 TCAACGC 6605 0.0 30.957022 4 TATCAAC 6845 0.0 30.011362 2 AACGCAG 6910 0.0 29.794647 6 GTACCTG 2250 0.0 29.671078 1 TACATGG 8965 0.0 28.458231 2 GTACATG 9450 0.0 27.907448 1 ACATGGG 9020 0.0 26.552341 3 ACGCAGA 8180 0.0 24.707159 7 CGCAGAG 8340 0.0 24.17759 8 CATGGGG 5455 0.0 23.600563 4 GTGTAGG 1175 0.0 21.35673 1 GTGTAGC 1195 0.0 20.999294 1 GCAGAGT 9745 0.0 20.254599 9 TAGCCCG 120 0.004863832 19.721365 5 GACCGTA 125 0.006157973 18.932512 7 >>END_MODULE