FastQCFastQC Report
Thu 26 May 2016
SRR936312_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936312_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2048039
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39810.19438106403247205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29360.14335664506388793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38100.038.9614221
GTATCAA60150.034.7243421
ATCAACG71500.028.8611873
TCAACGC71700.028.7145184
CAACGCA72800.028.2806455
TACATGG88850.027.6102372
TATCAAC74900.027.5577932
AACGCAG75450.027.2873546
GTACATG92950.027.0264931
ACATGGG91700.025.9178733
TACCTGG20300.025.711872
GTACCTG22650.023.3163171
ACGCAGA88550.023.0897737
CGCAGAG88700.023.0507268
CATGGGG54800.022.2476184
AGTACAT60950.022.0317862
GTATTAG4300.021.0227871
GAGTACA74550.020.2309741
GTACTTT46350.019.54950714-15
GCAGAGT104400.019.5388459
Produced by FastQC (version 0.11.5)