##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936312_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2048039 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.535180238266946 34.0 31.0 34.0 31.0 34.0 2 32.667873023902374 34.0 31.0 34.0 31.0 34.0 3 32.75197396143335 34.0 31.0 34.0 31.0 34.0 4 36.220604197478664 37.0 35.0 37.0 35.0 37.0 5 36.18462001944299 37.0 35.0 37.0 35.0 37.0 6 36.21856175590406 37.0 37.0 37.0 35.0 37.0 7 36.17026287097072 37.0 37.0 37.0 35.0 37.0 8 36.14755969002543 37.0 37.0 37.0 35.0 37.0 9 37.92559711997672 39.0 38.0 39.0 35.0 39.0 10-11 37.86724666864254 39.0 38.0 39.0 35.0 39.0 12-13 37.81112054018503 39.0 38.0 39.0 35.0 39.0 14-15 39.189984419242016 41.0 39.0 41.0 36.0 41.0 16-17 39.110847742645525 40.0 38.5 41.0 36.0 41.0 18-19 39.12168835652055 40.0 38.5 41.0 36.0 41.0 20-21 39.07292805459271 40.0 39.0 41.0 35.5 41.0 22-23 38.95533214943661 40.0 38.0 41.0 35.0 41.0 24-25 39.102686032834335 40.5 39.0 41.0 35.5 41.0 26-27 39.031070453248205 41.0 39.0 41.0 35.0 41.0 28-29 38.946618204047866 40.0 38.5 41.0 35.0 41.0 30-31 38.84018883429466 40.0 38.0 41.0 35.0 41.0 32-33 38.70131306093292 40.0 38.0 41.0 35.0 41.0 34-35 38.56917617291468 40.0 38.0 41.0 34.0 41.0 36-37 38.4283668426236 40.0 38.0 41.0 34.0 41.0 38-39 38.298506034308915 40.0 38.0 41.0 34.0 41.0 40-41 38.15492917859474 40.0 38.0 41.0 33.0 41.0 42-43 38.18895343301568 40.0 38.0 41.0 34.0 41.0 44-45 38.06514475554421 40.0 38.0 41.0 33.0 41.0 46-47 37.86979447168731 40.0 37.5 41.0 33.0 41.0 48-49 37.64302095809699 40.0 37.0 41.0 33.0 41.0 50-51 37.438378126588404 40.0 37.0 41.0 33.0 41.0 52-53 37.21600614050807 40.0 36.0 41.0 32.0 41.0 54-55 37.00775644409116 39.5 36.0 41.0 32.0 41.0 56-57 36.74842642156717 39.0 35.0 41.0 31.0 41.0 58-59 36.47354200774497 39.0 35.0 41.0 31.0 41.0 60-61 36.181206266091614 38.0 35.0 40.5 31.0 41.0 62-63 35.870136262053606 38.0 35.0 40.0 30.0 41.0 64-65 35.49573201486886 37.0 35.0 40.0 30.0 41.0 66-67 35.14000294916259 36.5 34.5 39.5 29.0 41.0 68-69 34.77818464394477 36.0 34.0 39.0 29.0 41.0 70-71 34.40801444699051 35.5 34.0 39.0 29.0 40.5 72-73 34.00604798053162 35.0 34.0 37.5 28.5 40.0 74-75 33.59278924864224 35.0 33.5 37.0 28.0 39.0 76-77 32.88009212715187 35.0 32.5 36.0 27.0 39.0 78-79 32.93505079737251 35.0 33.0 36.0 27.0 38.5 80-81 32.786648349958185 35.0 33.0 36.0 27.0 37.0 82-83 32.52830390436901 35.0 33.0 35.0 27.0 37.0 84-85 32.26162221520195 35.0 33.0 35.0 26.5 36.5 86-87 31.882189743457033 35.0 33.0 35.0 25.5 36.0 88-89 31.41381926809011 35.0 32.0 35.0 24.5 36.0 90-91 31.219015604683307 35.0 32.0 35.0 24.0 35.5 92-93 30.95110835291711 34.0 32.0 35.0 23.0 35.0 94-95 30.80399299036786 34.0 32.0 35.0 22.5 35.0 96-97 30.57011560815004 34.0 32.0 35.0 19.5 35.0 98-99 30.139838401514815 34.0 31.0 35.0 10.0 35.0 100-101 26.807786375161804 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21505026734867982 1101 2 0.1364763368276698 1101 3 0.17793437700948544 1101 4 0.19017468596383225 1101 5 0.08468378615257421 1101 6 0.14317504937908865 1101 7 0.1821915719156948 1101 8 0.10306453742668253 1101 9 0.24838719622123762 1101 10-11 0.19721059524665918 1101 12-13 0.32088591155415 1101 14-15 0.4240787953940668 1101 16-17 0.34548252827446646 1101 18-19 0.34983112171812536 1101 20-21 0.21062449446058906 1101 22-23 0.28018131171216254 1101 24-25 0.3078939784639445 1101 26-27 0.35127601600120073 1101 28-29 0.33852654187088405 1101 30-31 0.3650400198581778 1101 32-33 0.42621959769426354 1101 34-35 0.41023286342375087 1101 36-37 0.39441088502936594 1101 38-39 0.34373496732887787 1101 40-41 0.3889584182241208 1101 42-43 0.18996812889539427 1101 44-45 0.27389461296373696 1101 46-47 0.3542360330848737 1101 48-49 0.31995466444384135 1101 50-51 0.35847758466517377 1101 52-53 0.3620460483890042 1101 54-55 0.24642789160636625 1101 56-57 0.3096767136816183 1101 58-59 0.1287508876741228 1101 60-61 0.13295157113762457 1101 62-63 0.1374503049682474 1101 64-65 0.29375671225906075 1101 66-67 0.3060004808483967 1101 68-69 0.44376889088506033 1101 70-71 0.39504380578484444 1101 72-73 0.405929427670948 1101 74-75 0.25243438354292635 1101 76-77 0.2148073410613165 1101 78-79 0.18566741572002599 1101 80-81 0.28632052480888603 1101 82-83 0.2246258567311159 1101 84-85 0.3655933978840551 1101 86-87 0.35126696164692817 1101 88-89 0.5142441170239884 1101 90-91 0.6565056073236626 1101 92-93 0.6354133924924881 1101 94-95 0.5538227165009957 1101 96-97 0.4828940256720031 1101 98-99 0.5496653989688447 1101 100-101 0.972962522749345 1102 1 -0.039833080205305293 1102 2 0.12391383675071665 1102 3 0.10180371198773486 1102 4 0.07727870850018093 1102 5 0.02404839999720565 1102 6 0.04429103203850815 1102 7 0.055977122008712854 1102 8 0.04052229100678062 1102 9 0.04626554869397381 1102 10-11 0.08957379687581124 1102 12-13 0.18021702991308075 1102 14-15 0.2129998295248754 1102 16-17 0.16674277360842638 1102 18-19 0.34829609151001506 1102 20-21 0.23027731353570857 1102 22-23 0.18482290816819358 1102 24-25 0.10899490939343792 1102 26-27 0.13462575541235822 1102 28-29 0.14639875052910867 1102 30-31 0.18156943567868922 1102 32-33 0.13188486568892444 1102 34-35 0.1263330740864106 1102 36-37 0.13312978273793163 1102 38-39 0.2218302678648314 1102 40-41 0.14329787197868882 1102 42-43 0.16954425494331105 1102 44-45 0.18031194418428953 1102 46-47 0.2897036647859679 1102 48-49 0.27484025898409925 1102 50-51 0.17422132480767516 1102 52-53 0.1821055411980339 1102 54-55 0.20947574071724517 1102 56-57 0.30452542071031274 1102 58-59 0.16705984890160863 1102 60-61 0.15123434420186044 1102 62-63 0.15915957818823045 1102 64-65 0.2851181273802297 1102 66-67 0.30892340983501043 1102 68-69 0.09487256129095556 1102 70-71 0.11154462639654383 1102 72-73 0.03266360857980288 1102 74-75 0.09991280324056362 1102 76-77 -0.041696914669110186 1102 78-79 -0.04263177573428578 1102 80-81 0.19355077256835784 1102 82-83 0.26301176954311956 1102 84-85 0.29355409470835525 1102 86-87 0.220117452721162 1102 88-89 0.2892312199384186 1102 90-91 0.4934438145856781 1102 92-93 0.37021052964730217 1102 94-95 0.39685210338770815 1102 96-97 0.20991335837248215 1102 98-99 0.27423597834655666 1102 100-101 0.7178811018167899 1103 1 0.03372020999487546 1103 2 0.038970043371413965 1103 3 0.11027066258986196 1103 4 0.04126861187257447 1103 5 0.07386290685646912 1103 6 0.0867211764271758 1103 7 0.07808741702660882 1103 8 0.04104560632372056 1103 9 0.08769750106128527 1103 10-11 0.14398075967377366 1103 12-13 0.18938589464275424 1103 14-15 0.1444379451855795 1103 16-17 0.1206503135093655 1103 18-19 0.04014717160192305 1103 20-21 0.14156377770719075 1103 22-23 0.1262742954143974 1103 24-25 0.10899297779231887 1103 26-27 0.18053189029635774 1103 28-29 0.14010812004261908 1103 30-31 0.15146052903623541 1103 32-33 0.07983549634183618 1103 34-35 0.1736454145807329 1103 36-37 0.24213642269105407 1103 38-39 0.20932196198004505 1103 40-41 0.2819841573730457 1103 42-43 0.21938550704876292 1103 44-45 0.3053975289720725 1103 46-47 0.3988319956092141 1103 48-49 0.28386573947730653 1103 50-51 0.44243543179578637 1103 52-53 0.37310731113898754 1103 54-55 0.3590257965256569 1103 56-57 0.5185276566128962 1103 58-59 0.42540822891232466 1103 60-61 0.4935974174564919 1103 62-63 0.45935817649959176 1103 64-65 0.31924521009198514 1103 66-67 0.28401868234529815 1103 68-69 0.24078318343293148 1103 70-71 0.1854185623814999 1103 72-73 0.27953604920833897 1103 74-75 0.38825345090344143 1103 76-77 0.17086850717474533 1103 78-79 0.2221525506005051 1103 80-81 0.30486508151280134 1103 82-83 0.4610677467490376 1103 84-85 0.40328226196955796 1103 86-87 0.1705934289523654 1103 88-89 0.18138309489904358 1103 90-91 0.3182202540445438 1103 92-93 0.4842550824727283 1103 94-95 0.5291187867751042 1103 96-97 0.3729965087485212 1103 98-99 0.31924094701896166 1103 100-101 0.5145222852166711 1104 1 0.04833027846845539 1104 2 0.033152565545215396 1104 3 -0.04405228884237289 1104 4 0.016072266573239347 1104 5 0.02294385236797325 1104 6 -0.08396619697074215 1104 7 -0.06427058908383998 1104 8 0.007825971446735025 1104 9 -0.03940896673623939 1104 10-11 0.05706304249660832 1104 12-13 0.057813431687783634 1104 14-15 0.07968694422655176 1104 16-17 0.09828823530904174 1104 18-19 0.14740711785496075 1104 20-21 0.07648018418042568 1104 22-23 0.15576021720482203 1104 24-25 0.04127467270718199 1104 26-27 0.18001420273439805 1104 28-29 0.10209524784261248 1104 30-31 0.011080725676528402 1104 32-33 0.025988904229883758 1104 34-35 -0.010270755896378603 1104 36-37 -0.04214736379724826 1104 38-39 0.1089942570769935 1104 40-41 0.03406763290175263 1104 42-43 0.09261105339712827 1104 44-45 0.07204696830776669 1104 46-47 0.04818225433730561 1104 48-49 -0.004932340393494883 1104 50-51 0.04952358675731716 1104 52-53 -0.10342871702321332 1104 54-55 -0.008355230774704125 1104 56-57 -0.042722156846060955 1104 58-59 -0.05689668964009087 1104 60-61 -0.13520194651082562 1104 62-63 -0.06869427132386363 1104 64-65 -0.2550124432968133 1104 66-67 -0.2619110498106778 1104 68-69 -0.04834691058375995 1104 70-71 -0.06569576732589866 1104 72-73 -0.278677917134452 1104 74-75 -0.14229633328736213 1104 76-77 -0.1969336112374691 1104 78-79 -0.27518164766630804 1104 80-81 -0.1571615235789281 1104 82-83 -0.13831987803101953 1104 84-85 0.022543738726831464 1104 86-87 -0.0643101943978337 1104 88-89 -0.02638950664580264 1104 90-91 0.09083915154934985 1104 92-93 0.08094624916117965 1104 94-95 0.11539055134352338 1104 96-97 0.11367798802771745 1104 98-99 -0.02215303230361343 1104 100-101 0.1603246488797474 1105 1 -0.0917196004828682 1105 2 -0.02199334991369284 1105 3 0.008292104536025136 1105 4 0.007061673068449181 1105 5 -0.0444545888934158 1105 6 -0.004503936420633181 1105 7 0.05211173031305094 1105 8 0.09350015397268407 1105 9 1.2219734635721125E-4 1105 10-11 0.07506342047975068 1105 12-13 0.025595357651546635 1105 14-15 -0.0012258194886598517 1105 16-17 0.015674262418365004 1105 18-19 -0.015702868111773682 1105 20-21 -0.0062914242119447294 1105 22-23 -0.1632997393014577 1105 24-25 -0.017762815751645178 1105 26-27 0.06598173552215059 1105 28-29 -0.00871894664995665 1105 30-31 -0.03675680314611185 1105 32-33 -0.16106756776216713 1105 34-35 -0.03762051543810685 1105 36-37 0.15514116304625958 1105 38-39 0.10630903750043075 1105 40-41 0.20675889279708315 1105 42-43 0.2126594671649471 1105 44-45 0.07139119891966317 1105 46-47 0.1484362954929992 1105 48-49 0.2040175078573867 1105 50-51 0.20086036716764966 1105 52-53 0.21448731313903835 1105 54-55 0.20834954301550823 1105 56-57 0.27327160386666804 1105 58-59 0.1877105656407707 1105 60-61 0.28665588336995995 1105 62-63 0.2942847032928313 1105 64-65 0.3340135602897547 1105 66-67 0.21555063663833351 1105 68-69 0.2581059115050479 1105 70-71 0.14428241740295533 1105 72-73 0.0036265998780677933 1105 74-75 0.16123609872057187 1105 76-77 0.17398942301504405 1105 78-79 0.17077346241772062 1105 80-81 0.21844325656800834 1105 82-83 0.2090176925522016 1105 84-85 0.18342601864358699 1105 86-87 0.27973812473167925 1105 88-89 0.43154775442452475 1105 90-91 0.4941984695605868 1105 92-93 0.5834782515909893 1105 94-95 0.35654023010061664 1105 96-97 0.16231751953386464 1105 98-99 0.12749607905118765 1105 100-101 0.5512883433998184 1106 1 -0.07623431995851604 1106 2 0.04488796993728528 1106 3 -0.11972251690315261 1106 4 -0.01290667515709032 1106 5 0.035436170469068884 1106 6 0.010955574517431899 1106 7 -0.010388199660901876 1106 8 -0.04193722873367989 1106 9 0.002442300424704058 1106 10-11 0.04878012309312396 1106 12-13 -0.01875438045102129 1106 14-15 0.06272827143315851 1106 16-17 -0.05671810754326856 1106 18-19 0.007364973125170593 1106 20-21 -0.029875917643501282 1106 22-23 -0.07695691977259145 1106 24-25 0.027952850476189894 1106 26-27 -5.696215826915818E-4 1106 28-29 0.002105960215402547 1106 30-31 0.04736310946137934 1106 32-33 0.01983492796625086 1106 34-35 0.04317764147626946 1106 36-37 0.0627548153249009 1106 38-39 0.09590204609963138 1106 40-41 0.06779863958288956 1106 42-43 0.12264798289279355 1106 44-45 0.035066166007418076 1106 46-47 0.19076788284348822 1106 48-49 0.09523666138250064 1106 50-51 0.16221831232324746 1106 52-53 0.23262476026938117 1106 54-55 0.22461463362921563 1106 56-57 0.2564587694922835 1106 58-59 0.3121542787020317 1106 60-61 0.3070190384636575 1106 62-63 0.23478104793303345 1106 64-65 0.4224320919835378 1106 66-67 0.45528047613863265 1106 68-69 0.4710048499101376 1106 70-71 0.42417779848471326 1106 72-73 0.3216447226197303 1106 74-75 0.5487183821299979 1106 76-77 0.44913325652111524 1106 78-79 0.33369284842931535 1106 80-81 0.36558521337593675 1106 82-83 0.43755120701958816 1106 84-85 0.6177116128846478 1106 86-87 0.5719575303505735 1106 88-89 0.49184802612559864 1106 90-91 0.40755411786175344 1106 92-93 0.41302966471667446 1106 94-95 0.4089198553211979 1106 96-97 0.5578004191725299 1106 98-99 0.6552282154059093 1106 100-101 0.7818810438844004 1107 1 0.006878570540912676 1107 2 0.11666476060914022 1107 3 0.13004369485055634 1107 4 0.06722161614306543 1107 5 0.09097782861220338 1107 6 0.04612667829019301 1107 7 0.09355668476003842 1107 8 0.0928343893732162 1107 9 0.1804495478603414 1107 10-11 0.04983100126021611 1107 12-13 0.1244195872277487 1107 14-15 0.15516887460683648 1107 16-17 0.20824054116067003 1107 18-19 0.18192135992782 1107 20-21 0.17623209252629124 1107 22-23 0.18231497595784418 1107 24-25 0.18070994448829225 1107 26-27 0.20290067269827006 1107 28-29 0.19452128503494492 1107 30-31 0.04918563495890993 1107 32-33 0.17778518951684674 1107 34-35 0.2056626644167423 1107 36-37 0.203755867372017 1107 38-39 0.33039925525244485 1107 40-41 0.24052081147570448 1107 42-43 0.14278299560503882 1107 44-45 0.17311695650393233 1107 46-47 0.1626601064619564 1107 48-49 0.09132406896308254 1107 50-51 0.14961245008296942 1107 52-53 0.15920136975533694 1107 54-55 0.20220252339805 1107 56-57 0.14445856492756803 1107 58-59 0.19746550930491225 1107 60-61 0.1124717109555533 1107 62-63 0.10081921386424852 1107 64-65 0.253988537233262 1107 66-67 0.08299300426627809 1107 68-69 0.17067769248297537 1107 70-71 0.23079532091377786 1107 72-73 0.21980846206272275 1107 74-75 0.2261901083639586 1107 76-77 0.02596805590157203 1107 78-79 0.10004151437512832 1107 80-81 0.27041337901491147 1107 82-83 0.25767672208431947 1107 84-85 0.24067926243606053 1107 86-87 0.3314047672657381 1107 88-89 0.4281898245416862 1107 90-91 0.1616237867591508 1107 92-93 0.2731078376615308 1107 94-95 0.3008922975316679 1107 96-97 0.1560626419142821 1107 98-99 0.1568870891310361 1107 100-101 0.35942831294544675 1108 1 -0.32335428393488996 1108 2 -0.18737675074716265 1108 3 -0.19123339021123087 1108 4 -0.1400973619899304 1108 5 -0.052756555936781524 1108 6 -0.1020357109433192 1108 7 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-0.8715392132618902 2316 86-87 -0.8831827130604069 2316 88-89 -0.7643851486950659 2316 90-91 -0.8794084838953182 2316 92-93 -0.8235670145772716 2316 94-95 -0.6470493556081145 2316 96-97 -0.5832244055232039 2316 98-99 -0.7525621448745774 2316 100-101 -0.7924100820928714 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 22.0 5 40.0 6 68.0 7 131.0 8 343.0 9 569.0 10 927.0 11 1595.0 12 2261.0 13 3274.0 14 4212.0 15 5555.0 16 7171.0 17 9942.0 18 9319.0 19 6545.0 20 5983.0 21 6320.0 22 6946.0 23 7713.0 24 9333.0 25 11233.0 26 14010.0 27 17752.0 28 22224.0 29 27975.0 30 34713.0 31 43232.0 32 55170.0 33 72214.0 34 101935.0 35 154297.0 36 273439.0 37 501851.0 38 534435.0 39 95177.0 40 110.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.273416271315895 17.63690397505006 12.815729819974639 24.273949933659406 2 16.331858791722382 18.856811884108648 39.15489133354366 25.656437990625314 3 20.325931293300567 22.88476928417867 27.893170003110292 28.89612941941047 4 13.240958790335537 14.661390725469584 34.73864511369168 37.3590053705032 5 14.67232801719108 36.771809521205405 33.23930843113828 15.316554030465241 6 35.54170599290345 33.71493413943777 16.20100984405082 14.54235002360795 7 31.45613926297302 30.840672467662973 19.03362191833261 18.669566351031403 8 28.126075724143924 34.76545124384838 18.25058018914679 18.857892842860903 9 27.290447105743592 15.634956170268241 18.636412685500616 38.43818403848755 10-11 26.840387825782102 25.429131268880923 25.831663845260305 21.89881706007667 12-13 26.775077794004698 22.161831060669314 26.990430348670458 24.07266079665553 14-15 23.466935932372383 23.844931663898976 24.540645954495986 28.147486449232655 16-17 23.978594982628383 27.08219554990675 25.154020374267983 23.78518909319688 18-19 23.81155827598986 25.890962037344018 26.910229736836065 23.387249949830057 20-21 24.51800809311353 25.12078025915053 26.893965564595373 23.467246083140566 22-23 23.9529608107666 24.90982828923501 26.9478774065117 24.18933349348669 24-25 23.88435353223147 25.173719703728008 27.220782594386773 23.72114416965375 26-27 23.841985402258217 25.435647083869632 26.94302896306033 23.779338550811822 28-29 23.483098062002256 25.631033354655052 27.03707049350827 23.84879808983442 30-31 23.758036129103452 25.482387470999377 26.89018862885138 23.869387771045787 32-33 23.575650450072143 25.29827808037578 27.05598863284025 24.070082836711826 34-35 23.94862799239747 25.500554808893057 26.918009133289633 23.63280806541984 36-37 24.164493041633015 25.301678972753432 26.789249027605933 23.744578958007615 38-39 23.767240590077083 25.474240583973685 26.803659600959456 23.95485922498978 40-41 23.91169328830124 25.411970873345535 26.990348243499458 23.685987594853763 42-43 23.973256411539733 25.366027537058084 26.722640862220715 23.93807518918147 44-45 23.77584082396684 25.526594566801425 26.77467404544015 23.922890563791583 46-47 24.02243148749125 25.729307802503875 26.431176905930492 23.817083804074382 48-49 24.170584984960346 25.772364691355676 26.30299496497015 23.75405535871383 50-51 24.01474979045541 25.765817411891778 26.302759396423692 23.916673401229115 52-53 24.151694657442803 25.70420489121173 26.289542780157205 23.85455767118826 54-55 24.02985009037936 25.64146884280241 26.266800258198835 24.06188080861939 56-57 23.84528037566856 26.065887995242132 26.13769061202148 23.95114101706783 58-59 24.03394518818901 25.702811146931836 26.261891130769783 24.001352534109373 60-61 23.936210210116364 25.71930871363784 26.428095007441378 23.916386068804417 62-63 23.856991981109736 25.81957667798318 26.46704969973716 23.856381641169918 64-65 23.807303321684724 25.823658574813713 26.342084357286303 24.02695374621526 66-67 23.802183454514296 26.029069758925488 26.146474749748418 24.0222720368118 68-69 23.80298910323485 25.748386627403093 26.442831410925283 24.005792858436777 70-71 23.885273501051742 25.645179088648835 26.27585647908533 24.1936909312141 72-73 23.888287763938884 25.941962254127414 26.026556485020595 24.143193496913103 74-75 23.73746300729625 26.10155373017799 25.995623130223596 24.16536013230217 76-77 23.920583454519438 26.003011204567443 25.99662676596385 24.079778574949273 78-79 23.93331715434583 26.01270808145314 26.077106317210312 23.97686844699072 80-81 23.78508325842362 26.117896252040385 26.008400304489065 24.08862018504693 82-83 24.07373590053705 26.13700227388248 25.88681172575327 23.9024500998272 84-85 23.85193634158185 26.150517242347444 25.70171890033542 24.295827515735287 86-87 23.8406804792668 25.883213366710674 26.100299706746444 24.17580644727608 88-89 23.90574738952608 25.982092730949397 25.933515493879217 24.178644385645303 90-91 23.753784694100712 26.188405580591606 25.970954807023766 24.086854918283915 92-93 23.781452460234267 26.04801540457705 25.946349897318328 24.224182237870355 94-95 23.869226932449152 25.88029583145039 26.13522865462511 24.115248581475353 96-97 23.878944130313773 25.80764948876454 25.999814451313004 24.313591929608688 98-99 23.56787001441908 25.902901442933306 25.976925567180555 24.552302975467057 100-101 23.583371968143922 25.90895563454228 25.962397404131348 24.54527499318245 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 65.0 1 47.5 2 84.5 3 160.0 4 143.5 5 133.5 6 267.5 7 512.0 8 777.0 9 953.0 10 916.0 11 818.5 12 887.5 13 985.0 14 1060.5 15 1164.0 16 1256.5 17 1368.0 18 1462.5 19 1597.5 20 1864.5 21 2180.5 22 2563.5 23 3173.5 24 3887.0 25 4820.5 26 6435.5 27 8287.5 28 9874.0 29 11702.5 30 13898.0 31 17044.0 32 20827.0 33 23637.0 34 26207.0 35 29154.0 36 33463.5 37 37956.5 38 41705.0 39 44979.0 40 48338.5 41 52540.5 42 55423.5 43 59111.0 44 64191.5 45 68954.5 46 72832.0 47 75369.5 48 79851.0 49 82426.5 50 80222.0 51 80243.0 52 82515.0 53 84252.0 54 85990.5 55 83256.5 56 76909.0 57 70379.5 58 65380.0 59 60915.5 60 54063.0 61 46302.0 62 38567.0 63 29861.0 64 22380.0 65 17162.0 66 12435.0 67 10052.0 68 7789.5 69 5603.5 70 4447.5 71 3050.5 72 2321.5 73 1998.0 74 1562.5 75 1168.0 76 775.0 77 462.0 78 273.0 79 154.5 80 99.0 81 64.5 82 26.5 83 10.0 84 7.5 85 5.5 86 4.0 87 2.0 88 2.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2709421060829408 2 0.019384396488543433 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.929631711114876E-4 12-13 9.765439037049588E-5 14-15 0.0 16-17 7.07994330186095E-4 18-19 0.0 20-21 7.32407927778719E-5 22-23 9.765439037049588E-5 24-25 0.0027343229303738842 26-27 0.0024901869544476448 28-29 0.002392532564077149 30-31 0.0010986118916680786 32-33 0.00166012463629843 34-35 0.00178219262426155 36-37 4.882719518524794E-5 38-39 7.32407927778719E-5 40-41 8.788895133344629E-4 42-43 0.003491144455745228 44-45 0.0045165155546354335 46-47 0.014281954591685022 48-49 0.013940164225388286 50-51 0.007104356899453575 52-53 0.007910005620010166 54-55 2.4413597592623968E-4 56-57 0.003100526894263244 58-59 0.00153805664833531 60-61 0.0015136430507426862 62-63 0.0 64-65 5.859263422229752E-4 66-67 0.0 68-69 0.0 70-71 1.464815855557438E-4 72-73 0.0013183342700016944 74-75 0.0 76-77 0.19562615750969586 78-79 0.1062235631255069 80-81 8.788895133344629E-4 82-83 0.0 84-85 7.07994330186095E-4 86-87 0.12399666217293714 88-89 0.5911020249126115 90-91 0.3138367970531811 92-93 0.29533129007797215 94-95 0.002368118966484525 96-97 0.0028808045159296283 98-99 0.00283197732074438 100-101 7.568215253713431E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2048039.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.9716920358728 #Duplication Level Percentage of deduplicated Percentage of total 1 69.8826157534222 32.12622090077558 2 14.913196979749504 13.711697976467052 3 5.806370835182317 8.007860756432251 4 2.864556704198293 5.267540744987947 5 1.65983363052935 3.8152680246739976 6 1.055794510102155 2.9121996042948877 7 0.7259692965452021 2.3361825849792672 8 0.5144991967612512 1.8921918900969732 9 0.37283347264872085 1.5425807026744798 >10 1.9843441805042175 16.47712379301609 >50 0.14547059825246353 4.584519102515862 >100 0.07047683599624842 5.664212837670131 >500 0.003400426196296483 1.0278259992060799 >1k 6.375799118055897E-4 0.6345750822094562 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3981 0.19438106403247205 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2936 0.14335664506388793 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 4.8827195185247934E-5 0.0 7 0.0 0.0 0.0 4.8827195185247934E-5 0.0 8 0.0 0.0 0.0 4.8827195185247934E-5 0.0 9 0.0 0.0 0.0 4.8827195185247934E-5 0.0 10-11 0.0 0.0 0.0 4.8827195185247934E-5 0.0 12-13 0.0 0.0 0.0 4.8827195185247934E-5 2.4413597592623967E-5 14-15 0.0 0.0 0.0 4.8827195185247934E-5 1.4648158555574382E-4 16-17 0.0 0.0 0.0 9.765439037049587E-5 1.4648158555574382E-4 18-19 0.0 0.0 0.0 1.4648158555574382E-4 1.4648158555574382E-4 20-21 0.0 0.0 0.0 1.4648158555574382E-4 1.4648158555574382E-4 22-23 0.0 0.0 0.0 2.685495735188637E-4 1.4648158555574382E-4 24-25 0.0 0.0 0.0 4.394447566672314E-4 1.4648158555574382E-4 26-27 0.0 0.0 0.0 5.859263422229753E-4 1.9530878074099174E-4 28-29 0.0 0.0 0.0 0.0010741982940754547 1.9530878074099174E-4 30-31 0.0 0.0 0.0 0.0025634277472255168 1.9530878074099174E-4 32-33 0.0 0.0 0.0 0.0054442322631551455 1.9530878074099174E-4 34-35 0.0 0.0 0.0 0.009911920622605331 1.9530878074099174E-4 36-37 0.0 0.0 0.0 0.016747727948540042 2.197223783336157E-4 38-39 0.0 0.0 0.0 0.029052181135222524 2.4413597592623968E-4 40-41 0.0 0.0 0.0 0.044042130057093634 2.4413597592623968E-4 42-43 0.0 0.0 0.0 0.05905649257655737 2.4413597592623968E-4 44-45 0.0 0.0 0.0 0.07695165961195075 2.4413597592623968E-4 46-47 0.0 0.0 0.0 0.09677550085716141 2.4413597592623968E-4 48-49 0.0 0.0 0.0 0.12206798796311985 2.9296317111148764E-4 50-51 0.0 0.0 0.0 0.1506318971464899 2.9296317111148764E-4 52-53 0.0 0.0 0.0 0.1789760839515263 2.9296317111148764E-4 54-55 0.0 0.0 0.0 0.20927335856397267 2.9296317111148764E-4 56-57 0.0 0.0 0.0 0.24709002123494717 2.9296317111148764E-4 58-59 0.0 0.0 0.0 0.28930113147259406 2.9296317111148764E-4 60-61 0.0 0.0 0.0 0.3369076467782108 2.9296317111148764E-4 62-63 0.0 0.0 0.0 0.3826831422643807 3.9061756148198347E-4 64-65 0.0 0.0 0.0 0.43270660373166725 4.394447566672314E-4 66-67 0.0 0.0 0.0 0.4914701331371131 4.394447566672314E-4 68-69 0.0 0.0 0.0 0.5613418494472029 4.394447566672314E-4 70-71 0.0 0.0 0.0 0.6396118433291553 4.394447566672314E-4 72-73 0.0 0.0 0.0 0.7196396162377767 4.394447566672314E-4 74-75 0.0 0.0 0.0 0.801107791404363 4.394447566672314E-4 76-77 0.0 0.0 0.0 0.8915601704850347 4.394447566672314E-4 78-79 0.0 0.0 0.0 0.9954400282416498 4.394447566672314E-4 80-81 0.0 0.0 0.0 1.1140412853466168 4.394447566672314E-4 82-83 0.0 0.0 0.0 1.2428962534404864 4.394447566672314E-4 84-85 0.0 0.0 0.0 1.3681868362858325 4.394447566672314E-4 86-87 0.0 0.0 0.0 1.504805328414156 4.882719518524794E-4 88-89 0.0 0.0 0.0 1.6608814578238014 5.370991470377273E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3810 0.0 38.961422 1 GTATCAA 6015 0.0 34.724342 1 ATCAACG 7150 0.0 28.861187 3 TCAACGC 7170 0.0 28.714518 4 CAACGCA 7280 0.0 28.280645 5 TACATGG 8885 0.0 27.610237 2 TATCAAC 7490 0.0 27.557793 2 AACGCAG 7545 0.0 27.287354 6 GTACATG 9295 0.0 27.026493 1 ACATGGG 9170 0.0 25.917873 3 TACCTGG 2030 0.0 25.71187 2 GTACCTG 2265 0.0 23.316317 1 ACGCAGA 8855 0.0 23.089773 7 CGCAGAG 8870 0.0 23.050726 8 CATGGGG 5480 0.0 22.247618 4 AGTACAT 6095 0.0 22.031786 2 GTATTAG 430 0.0 21.022787 1 GAGTACA 7455 0.0 20.230974 1 GTACTTT 4635 0.0 19.549507 14-15 GCAGAGT 10440 0.0 19.538845 9 >>END_MODULE