FastQCFastQC Report
Thu 26 May 2016
SRR936311_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936311_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3201822
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37000.040.4335141
GTATCAA60450.036.0261341
GTACATG118600.030.497541
TACATGG118150.030.164442
ATCAACG71800.029.8603023
TCAACGC72850.029.429464
CAACGCA73200.029.4180565
ACATGGG119200.028.6669433
AACGCAG76350.028.2021436
TATCAAC79000.027.3194392
CATGGGG82300.024.842634
ACGCAGA93050.022.7340957
GAGTACA83850.022.1894041
CGCAGAG99600.021.2400268
GTATAGC11600.020.8146131
AGTACAT84150.020.1917672
GTACCTG29950.020.0753461
TCTATAC7750.019.5419863
GTATAGG7450.019.064311
GCAGAGT110400.018.1762249
Produced by FastQC (version 0.11.5)