##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936311_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3201822 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.755777179368497 33.0 31.0 34.0 30.0 34.0 2 31.846131671279664 34.0 31.0 34.0 30.0 34.0 3 31.917661881266355 34.0 31.0 34.0 30.0 34.0 4 35.41018801170084 37.0 35.0 37.0 33.0 37.0 5 35.39132437718274 37.0 35.0 37.0 33.0 37.0 6 35.48025280605855 37.0 35.0 37.0 33.0 37.0 7 35.42209716842473 37.0 35.0 37.0 33.0 37.0 8 35.42071014566081 37.0 35.0 37.0 33.0 37.0 9 37.09980910868874 39.0 37.0 39.0 34.0 39.0 10-11 37.01683978684636 39.0 37.0 39.0 33.0 39.0 12-13 36.922625929861184 39.0 37.0 39.0 33.0 39.0 14-15 38.19405825807931 40.0 38.0 41.0 33.0 41.0 16-17 38.13305627233494 40.0 38.0 41.0 33.0 41.0 18-19 38.057741498434325 40.0 38.0 41.0 33.0 41.0 20-21 37.964646847950945 40.0 38.0 41.0 33.0 41.0 22-23 37.80584679598054 40.0 38.0 41.0 32.5 41.0 24-25 37.962722787213025 40.0 38.0 41.0 33.0 41.0 26-27 37.92280895065372 40.0 38.0 41.0 33.0 41.0 28-29 37.793932329779736 40.0 38.0 41.0 32.5 41.0 30-31 37.65921247339796 40.0 38.0 41.0 32.0 41.0 32-33 37.49731512245215 40.0 37.5 41.0 32.0 41.0 34-35 37.245647790539266 40.0 37.0 41.0 31.5 41.0 36-37 37.053636023489126 40.0 37.0 41.0 31.0 41.0 38-39 36.89979486679772 40.0 37.0 41.0 30.5 41.0 40-41 36.724044622093295 40.0 36.0 41.0 30.0 41.0 42-43 36.544570091654066 40.0 36.0 41.0 30.0 41.0 44-45 36.378553211265334 40.0 36.0 41.0 30.0 41.0 46-47 36.140939440106294 39.0 35.0 41.0 29.5 41.0 48-49 35.99930492700719 39.0 35.0 41.0 29.0 41.0 50-51 35.68901800287462 38.5 35.0 40.5 28.5 41.0 52-53 35.69399610596716 39.0 35.0 40.5 28.0 41.0 54-55 35.74838779295039 39.0 35.0 41.0 28.0 41.0 56-57 35.558974702528744 39.0 35.0 41.0 28.0 41.0 58-59 35.304776311737506 38.5 35.0 40.5 28.0 41.0 60-61 34.98948598641648 38.0 34.5 40.0 27.5 41.0 62-63 34.662712199491416 37.0 34.0 40.0 27.0 41.0 64-65 34.31723078297294 37.0 34.0 40.0 26.0 41.0 66-67 33.944595764536565 36.0 34.0 39.0 26.0 41.0 68-69 33.55815391986188 35.5 34.0 39.0 26.0 41.0 70-71 33.15687739668226 35.0 33.0 38.5 25.5 40.0 72-73 32.76644797868214 35.0 33.0 37.0 24.5 40.0 74-75 32.367662693304 35.0 33.0 37.0 24.0 39.0 76-77 31.988984865492213 35.0 32.5 36.0 23.0 39.0 78-79 31.62249650355329 35.0 32.0 36.0 21.5 38.0 80-81 31.258268417169973 35.0 32.0 35.5 20.0 37.0 82-83 30.9399451312409 35.0 31.5 35.0 19.5 37.0 84-85 30.653110947454294 35.0 31.0 35.0 18.0 36.0 86-87 30.357401036035107 34.0 31.0 35.0 16.0 36.0 88-89 30.05099549568964 34.0 31.0 35.0 9.0 36.0 90-91 29.723763375977803 34.0 30.5 35.0 4.5 35.0 92-93 29.42991584166765 34.0 30.0 35.0 2.0 35.0 94-95 29.066520874676982 34.0 30.0 35.0 2.0 35.0 96-97 28.652770360126205 34.0 29.0 35.0 2.0 35.0 98-99 28.15858314422226 34.0 29.0 35.0 2.0 35.0 100-101 24.84217829723201 30.0 23.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3386538614518706 1101 2 0.2833609566220012 1101 3 0.41151955775749016 1101 4 0.14024901550881452 1101 5 0.38476750332610976 1101 6 0.32398909066110804 1101 7 0.4239329918886128 1101 8 0.3973866138182487 1101 9 0.4073831097162781 1101 10-11 0.577867915233135 1101 12-13 0.4662972321118559 1101 14-15 0.6088867562985882 1101 16-17 0.6173049382652138 1101 18-19 0.6045761919402679 1101 20-21 0.6754689993444956 1101 22-23 0.7195879934444136 1101 24-25 0.6646681484077348 1101 26-27 0.6629626578327148 1101 28-29 0.5748813566447595 1101 30-31 0.6698658244762541 1101 32-33 0.6537695051660393 1101 34-35 0.4705864120734802 1101 36-37 0.4205691925060435 1101 38-39 0.34677390524021945 1101 40-41 0.33180385502563325 1101 42-43 0.42591093250930356 1101 44-45 0.4944048494129518 1101 46-47 0.3733772301195373 1101 48-49 0.4166434840603799 1101 50-51 0.5341532033864596 1101 52-53 0.5979134718492602 1101 54-55 0.5576911268741895 1101 56-57 0.5813002173390487 1101 58-59 0.573198356810515 1101 60-61 0.44761569332272444 1101 62-63 0.4281701798042903 1101 64-65 0.4170177611503689 1101 66-67 0.43413158171749444 1101 68-69 0.5812532724532957 1101 70-71 0.7009413529526896 1101 72-73 0.6942279252627159 1101 74-75 0.5526199612966778 1101 76-77 0.5078405331847264 1101 78-79 0.5939802133362448 1101 80-81 0.6287161694081433 1101 82-83 0.7658832502840447 1101 84-85 0.6787313751445332 1101 86-87 0.5442494466823611 1101 88-89 0.562123585571868 1101 90-91 0.6037256690489166 1101 92-93 0.5393946228980582 1101 94-95 0.7736347462247615 1101 96-97 0.9003579656264442 1101 98-99 1.0535238614360658 1101 100-101 1.1459850828100748 1102 1 0.2724488012821986 1102 2 0.3423499769594436 1102 3 0.3437013304789822 1102 4 0.3134454253074068 1102 5 0.3791940461075072 1102 6 0.3230891844653314 1102 7 0.31114444498461324 1102 8 0.2754560924596632 1102 9 0.2851702776057863 1102 10-11 0.4028105192055591 1102 12-13 0.46220789123653105 1102 14-15 0.5424947254534374 1102 16-17 0.5574577921613937 1102 18-19 0.4824618236766298 1102 20-21 0.49282468082559916 1102 22-23 0.4969625372925819 1102 24-25 0.5523944379164263 1102 26-27 0.5168287727721079 1102 28-29 0.4780261864858275 1102 30-31 0.49127192985951496 1102 32-33 0.2614743760222069 1102 34-35 0.31563178028336125 1102 36-37 0.2700728969723727 1102 38-39 0.3118161158388304 1102 40-41 0.2637850556546013 1102 42-43 0.2712222767549193 1102 44-45 0.30306863969445885 1102 46-47 0.35870520583950594 1102 48-49 0.32971812928791167 1102 50-51 0.2525551266919095 1102 52-53 0.3515890107614865 1102 54-55 0.29567004480792036 1102 56-57 0.3378984990310343 1102 58-59 0.33321931470690913 1102 60-61 0.35379253282977885 1102 62-63 0.39508990658370635 1102 64-65 0.4064730185405949 1102 66-67 0.4002471979274205 1102 68-69 0.45905332589580894 1102 70-71 0.49051526155611214 1102 72-73 0.3716672613157215 1102 74-75 0.4265042383579356 1102 76-77 0.3690279199724458 1102 78-79 0.34223620580171144 1102 80-81 0.34258270345340236 1102 82-83 0.43352982637785686 1102 84-85 0.49980258630711916 1102 86-87 0.5398738252635553 1102 88-89 0.4071362843494164 1102 90-91 0.43938052781999914 1102 92-93 0.5068546318034066 1102 94-95 0.3992731179958753 1102 96-97 0.5968595249735884 1102 98-99 0.5161946747344253 1102 100-101 0.5434193349267211 1103 1 0.2413002649130611 1103 2 0.1792507276668296 1103 3 0.22315475056743495 1103 4 0.10625081585330776 1103 5 0.13476747115412024 1103 6 0.07544207419483939 1103 7 0.20996159793424596 1103 8 0.16916508779786454 1103 9 0.16597171169537006 1103 10-11 0.23583302406775886 1103 12-13 0.3755111610387871 1103 14-15 0.4063259946717963 1103 16-17 0.39228561898588765 1103 18-19 0.3047870471553793 1103 20-21 0.3513494662501344 1103 22-23 0.4247492812762559 1103 24-25 0.5375070754847684 1103 26-27 0.4855111683661377 1103 28-29 0.36873927355995306 1103 30-31 0.4029188110985018 1103 32-33 0.33670342325743974 1103 34-35 0.12515911336878816 1103 36-37 0.1540614554644364 1103 38-39 0.2659822555442801 1103 40-41 0.22753276612606044 1103 42-43 0.12849569443979902 1103 44-45 0.27353867652197295 1103 46-47 0.2880110453249145 1103 48-49 0.30228229428875864 1103 50-51 0.1900499750560627 1103 52-53 0.31580013250620453 1103 54-55 0.3788389976997877 1103 56-57 0.3331180955706472 1103 58-59 0.4092843898562961 1103 60-61 0.4500011176101353 1103 62-63 0.4981874947687004 1103 64-65 0.4406887523757774 1103 66-67 0.5097308010963602 1103 68-69 0.49571258679100083 1103 70-71 0.3925014177342021 1103 72-73 0.3258890229581013 1103 74-75 0.3758137775830406 1103 76-77 0.29903570326838036 1103 78-79 0.23533531037661604 1103 80-81 0.260985510725277 1103 82-83 0.35418532209578757 1103 84-85 0.30757864634860965 1103 86-87 0.4128965566404297 1103 88-89 0.31032655478906435 1103 90-91 0.49802511433948027 1103 92-93 0.41783404979911865 1103 94-95 0.37876900958594817 1103 96-97 0.5391735988966566 1103 98-99 0.5904666345456882 1103 100-101 0.6241711874707008 1104 1 0.13641127683719745 1104 2 0.11855573691910948 1104 3 0.12268330246856962 1104 4 0.16018683405412304 1104 5 0.11941952633927144 1104 6 0.048382007181636766 1104 7 0.1295556752318987 1104 8 0.17400541226326283 1104 9 0.1167061602622681 1104 10-11 0.1159804407119509 1104 12-13 0.15760574202030853 1104 14-15 0.288709457723094 1104 16-17 0.2675846522228227 1104 18-19 0.03142123008716169 1104 20-21 0.272169432588548 1104 22-23 0.29717747145488715 1104 24-25 0.3414801338222091 1104 26-27 0.242004660770597 1104 28-29 0.26371607310617406 1104 30-31 0.1393694051355041 1104 32-33 0.23455788496661967 1104 34-35 0.15175238167265803 1104 36-37 0.16980397847117246 1104 38-39 0.03897519157177243 1104 40-41 0.12851967654471963 1104 42-43 0.12520454656752378 1104 44-45 0.12797930846699757 1104 46-47 0.07451634717878619 1104 48-49 0.07724905115358638 1104 50-51 0.018136317319324746 1104 52-53 0.15239764725252058 1104 54-55 0.1906165213109432 1104 56-57 0.2140825649358149 1104 58-59 0.13937120945688264 1104 60-61 0.12500909906217572 1104 62-63 0.2595761744350611 1104 64-65 0.24409458518993432 1104 66-67 0.16637431380313927 1104 68-69 0.029517058263891727 1104 70-71 0.044233315336050794 1104 72-73 0.1901556747467268 1104 74-75 0.15801002521572372 1104 76-77 0.2376464036834136 1104 78-79 0.07033715463446555 1104 80-81 0.08699419578492851 1104 82-83 0.20648072570929088 1104 84-85 -0.03913748982271059 1104 86-87 0.08967409574954388 1104 88-89 -0.01321888369486146 1104 90-91 0.10974312576458445 1104 92-93 0.10328827965873799 1104 94-95 -0.2169991160591529 1104 96-97 -0.013681591216879951 1104 98-99 0.09871403166244619 1104 100-101 0.29593606474231393 1105 1 0.14606132610277456 1105 2 0.20274553879403 1105 3 0.17431987440081897 1105 4 0.07855055633434915 1105 5 0.1261862932983746 1105 6 0.14203920641092083 1105 7 0.268675427516996 1105 8 0.2616456660545481 1105 9 0.2708311093004667 1105 10-11 0.2888150818898083 1105 12-13 0.2801382750156307 1105 14-15 0.2256306622169788 1105 16-17 0.3386050231346971 1105 18-19 0.34394765667244087 1105 20-21 0.22198212731668576 1105 22-23 0.3461484783160458 1105 24-25 0.4041250559849985 1105 26-27 0.39738703792337304 1105 28-29 0.44080034336615626 1105 30-31 0.5455760876015816 1105 32-33 0.3760757091392293 1105 34-35 0.172495022007503 1105 36-37 0.2970559198232152 1105 38-39 0.3329508608000893 1105 40-41 0.3560709941062683 1105 42-43 0.25482213639089224 1105 44-45 0.29575565654557323 1105 46-47 0.32490539815139385 1105 48-49 0.3659242123549973 1105 50-51 0.3220048511744764 1105 52-53 0.41262396269753054 1105 54-55 0.4163056540025707 1105 56-57 0.48794010485114114 1105 58-59 0.4978707978596262 1105 60-61 0.4368678434473665 1105 62-63 0.33877078878554556 1105 64-65 0.35990442305484294 1105 66-67 0.49599226388668427 1105 68-69 0.4949603614174407 1105 70-71 0.5395224931802929 1105 72-73 0.5099393625260333 1105 74-75 0.5580838785526083 1105 76-77 0.5367864626339909 1105 78-79 0.3953415528108337 1105 80-81 0.516623133803968 1105 82-83 0.43576369386889624 1105 84-85 0.46960524417978533 1105 86-87 0.47342792276341683 1105 88-89 0.5164152780967832 1105 90-91 0.6003166179406314 1105 92-93 0.3191593017108616 1105 94-95 0.3848383909884383 1105 96-97 0.27134587561966583 1105 98-99 0.39025049929937694 1105 100-101 0.5190717333221606 1106 1 0.12861924049494888 1106 2 0.1185681134630876 1106 3 0.14868571412656806 1106 4 0.1593645600468676 1106 5 0.13299883464370055 1106 6 0.03419707332542998 1106 7 0.05660938589658571 1106 8 -0.013789429428953781 1106 9 0.07862789571305484 1106 10-11 0.11332637926064137 1106 12-13 0.14107739796652652 1106 14-15 0.19898193028547695 1106 16-17 0.19782502828116577 1106 18-19 0.04435159493588969 1106 20-21 0.1573931356740843 1106 22-23 0.13945604034921644 1106 24-25 0.16703734351268906 1106 26-27 0.12343624971845202 1106 28-29 0.1507051428497661 1106 30-31 0.1818988642569579 1106 32-33 0.1399128029926402 1106 34-35 0.004659572916196453 1106 36-37 0.08871133002041631 1106 38-39 0.08126531190714559 1106 40-41 -0.046721400851112094 1106 42-43 -0.0027050905168479744 1106 44-45 -0.04591508307263581 1106 46-47 0.07224360115372264 1106 48-49 0.09632059818621741 1106 50-51 0.1863487553924088 1106 52-53 0.11360796716849819 1106 54-55 0.1409797434086144 1106 56-57 0.15858944216875415 1106 58-59 0.004296437450506119 1106 60-61 0.1347751941682489 1106 62-63 0.022824203912456653 1106 64-65 0.04341747036271215 1106 66-67 0.02075248676592878 1106 68-69 -0.018873696339881008 1106 70-71 0.06254016875887913 1106 72-73 0.15405058492248003 1106 74-75 0.12888901915593465 1106 76-77 0.05651638985704466 1106 78-79 0.06465443500090373 1106 80-81 0.16766633642018647 1106 82-83 0.2644208090918383 1106 84-85 0.27220343210015585 1106 86-87 0.3319280294277682 1106 88-89 0.2317029613767403 1106 90-91 0.35534061226530866 1106 92-93 0.23274822621564084 1106 94-95 0.2582905834737197 1106 96-97 0.2636091007969483 1106 98-99 0.3344366273419723 1106 100-101 0.4470467122241608 1107 1 0.03864464646359522 1107 2 0.0962070141991731 1107 3 0.18117240484444253 1107 4 0.10252644072411243 1107 5 0.05705522619332726 1107 6 0.12879458357394924 1107 7 0.06315489335530344 1107 8 0.14153486881269828 1107 9 0.1612867750071061 1107 10-11 0.13264302727842647 1107 12-13 0.12066063303780084 1107 14-15 0.2693588886273801 1107 16-17 0.06103086345847686 1107 18-19 0.2195829498149564 1107 20-21 0.2906256145038313 1107 22-23 0.2946235374348021 1107 24-25 0.28561915859270215 1107 26-27 0.24642062312430824 1107 28-29 0.3521431976122571 1107 30-31 0.24746265431233638 1107 32-33 0.3288627955498171 1107 34-35 0.17295320837818196 1107 36-37 0.1619328704015217 1107 38-39 0.1845922561160407 1107 40-41 0.22149011261873142 1107 42-43 -0.09554517340821178 1107 44-45 0.22613074133348476 1107 46-47 0.3370665371069279 1107 48-49 0.2112295029865905 1107 50-51 0.1834109289323962 1107 52-53 0.22601181669959658 1107 54-55 0.3229359862689307 1107 56-57 0.2700746459935317 1107 58-59 0.40487697805071576 1107 60-61 0.334143055534291 1107 62-63 0.3542893037383408 1107 64-65 0.17804861550455797 1107 66-67 0.34620311238163737 1107 68-69 0.37199102649421434 1107 70-71 0.41519600401981904 1107 72-73 0.25148640516396625 1107 74-75 0.31768221055704515 1107 76-77 0.4056360454464354 1107 78-79 0.23742840216214134 1107 80-81 0.277663392739818 1107 82-83 0.5294310701594895 1107 84-85 0.4832611332530412 1107 86-87 0.17651968576646837 1107 88-89 0.4464373360370004 1107 90-91 0.6247990085281749 1107 92-93 0.36449297567666505 1107 94-95 0.461522443461579 1107 96-97 0.38938537105356374 1107 98-99 0.3775707437611828 1107 100-101 0.4931847206571369 1108 1 -0.09706093212757949 1108 2 0.06903384716240879 1108 3 0.034554922989972425 1108 4 0.029003265549320645 1108 5 0.0069353182953406645 1108 6 0.0011341590988536154 1108 7 -0.06584289970198398 1108 8 -0.004241835023329088 1108 9 0.11318111492180805 1108 10-11 -0.05392023139370394 1108 12-13 0.05234596785228973 1108 14-15 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-1.6562722326949029 2316 100-101 -1.6196974249560583 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24958.0 3 5614.0 4 4804.0 5 4158.0 6 4033.0 7 4437.0 8 5005.0 9 5962.0 10 6979.0 11 8430.0 12 10459.0 13 14641.0 14 15117.0 15 11836.0 16 13237.0 17 15528.0 18 14817.0 19 12741.0 20 12861.0 21 13811.0 22 15011.0 23 16526.0 24 19557.0 25 23091.0 26 27926.0 27 33445.0 28 40535.0 29 48873.0 30 60163.0 31 75046.0 32 96422.0 33 127004.0 34 178799.0 35 271147.0 36 461294.0 37 740298.0 38 652292.0 39 104922.0 40 43.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.29807942285194 17.243791808737214 12.374511293983542 25.0836174744273 2 16.402502639453743 18.617207364232122 38.60558752053776 26.374702475776374 3 20.162468709764678 22.1332459570236 27.800690805934007 29.903594527277715 4 13.253699195600257 14.37434291529159 34.679455104599455 37.6925027845087 5 14.955872867631099 36.373563388926286 33.23542227268397 15.435141470758648 6 35.991516834367445 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24.301269796698534 28-29 23.98601670566968 25.548985028809852 26.179051704358468 24.285946561161996 30-31 24.103198370436964 25.307909259502793 26.158894266135935 24.429998103924312 32-33 24.028355803517254 25.22902157671799 26.188292243636006 24.554330376128746 34-35 24.443166385940927 25.38849994746668 26.03566973249345 24.132663934098947 36-37 24.66332138935127 25.140275236166527 25.927128268074483 24.26927510640772 38-39 24.255029387122384 25.306438807946602 25.933970760362374 24.504561044568636 40-41 24.40844952171112 25.236829695261992 26.097082716395214 24.25763806663167 42-43 24.375837927813933 25.25253951755443 25.878882712701856 24.49273984192978 44-45 24.172277152204956 25.353849408546875 26.065856237333936 24.40801720191423 46-47 24.441236024132106 25.54929200341085 25.749498267970967 24.259973704486075 48-49 24.589800603554334 25.443758097331532 25.696641273183836 24.269800025930298 50-51 24.426588294539716 25.6021231051889 25.673799568371436 24.29748903189995 52-53 24.659588063533203 25.436306933681536 25.633276623516664 24.2708283792686 54-55 24.410324594745045 25.448986346671838 25.707608132202676 24.433080926380445 56-57 24.250708352001705 25.699865901570657 25.621698051767172 24.42772769466047 58-59 24.43641352262302 25.33836217110456 25.692330917779632 24.53289338849278 60-61 24.34232123835869 25.594470294845213 25.825209444738412 24.237999022057682 62-63 24.218932297449022 25.545945920499385 25.962051923413398 24.273069858638195 64-65 24.064497437222222 25.574729871702594 25.783091913148372 24.57768077792681 66-67 24.114370639360878 25.81488947176751 25.65941391252856 24.411325976343047 68-69 24.2824350110129 25.375924922052768 25.98201900992809 24.359621057006244 70-71 24.2036512472626 25.336067019455704 25.796788117566493 24.6634936157152 72-73 24.206297954932467 25.740698863621205 25.548208257071785 24.504794924374544 74-75 24.127741537329545 25.8063068111011 25.621384783783114 24.444566867786243 76-77 24.307815148706556 25.651382706230518 25.58749596294161 24.453306182121317 78-79 24.424316913821414 25.679539553966446 25.577989961483027 24.318153570729113 80-81 24.356316932618547 25.805529748527956 25.483154637104366 24.35499868174913 82-83 24.44261074849582 25.88162439444746 25.570971958419182 24.10479289863754 84-85 24.384039974607838 25.888658978397043 25.33632452375964 24.390976523235476 86-87 24.25263898640825 25.67061846334735 25.632953408012675 24.443789142231722 88-89 24.332428887295936 25.758677557270556 25.462732684792105 24.446160870641407 90-91 24.146492335324076 25.917468929959746 25.494545865596564 24.441492869119614 92-93 24.193588138179646 25.75442833266382 25.61529802346425 24.43668550569229 94-95 24.288009546563348 25.57208446272961 25.777808257701967 24.36209773300507 96-97 24.356629067664397 25.475829033525322 25.662134871934544 24.505407026875737 98-99 24.20511961326175 25.586520384830663 25.528367205560116 24.67999279634747 100-101 24.192848955370376 25.64621190560538 25.385599696760412 24.77533944226383 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 73.0 1 64.5 2 107.5 3 188.0 4 170.0 5 149.5 6 308.5 7 593.0 8 915.0 9 1049.0 10 947.0 11 960.5 12 1189.5 13 1485.5 14 1697.5 15 1912.0 16 2215.0 17 2449.5 18 2524.5 19 2573.0 20 2593.0 21 2627.5 22 2777.0 23 3069.0 24 3691.5 25 4738.0 26 6264.5 27 8343.0 28 10970.0 29 14249.0 30 18305.0 31 23843.5 32 30835.0 33 34880.0 34 37307.5 35 42026.5 36 48401.0 37 54393.5 38 60204.0 39 66010.0 40 72172.0 41 79239.5 42 85601.5 43 92892.5 44 99142.5 45 106093.5 46 112721.5 47 118808.0 48 125297.5 49 126775.0 50 126175.0 51 127283.5 52 130868.5 53 135155.5 54 137898.5 55 134417.5 56 126005.0 57 117273.0 58 111497.5 59 103292.5 60 88879.0 61 75247.0 62 63579.0 63 50459.0 64 38575.0 65 29849.5 66 21429.5 67 16821.5 68 13150.0 69 9290.0 70 6945.5 71 5112.5 72 4265.0 73 3696.5 74 2792.0 75 2062.5 76 1432.5 77 819.0 78 493.0 79 295.5 80 170.5 81 103.0 82 77.0 83 65.5 84 53.5 85 45.0 86 41.0 87 39.5 88 36.0 89 32.0 90 27.5 91 28.0 92 37.0 93 36.5 94 28.0 95 22.0 96 25.0 97 29.5 98 25.5 99 18.5 100 10.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04791022111785102 2 0.012555351296855352 3 0.01074388270178667 4 0.005621799088144188 5 0.008651324152310777 6 0.005840424608238684 7 0.005590566870987831 8 0.009744451752783259 9 0.014460516543393106 10-11 0.0024048807210394585 12-13 0.009135423518234305 14-15 0.009525826232688763 16-17 0.020222860608740897 18-19 0.012149332473822718 20-21 0.0037790982759191485 22-23 0.010056773924346825 24-25 0.02276828630698396 26-27 0.0076987415290419026 28-29 0.017911676539170508 30-31 0.01477283871495667 32-33 0.013476701702967874 34-35 0.2689250058248085 36-37 0.47287138385581706 38-39 0.4701073326374795 40-41 0.49111099867512936 42-43 0.48066382203632807 44-45 0.49651417224317906 46-47 0.5030729378460139 48-49 0.5109278404608376 50-51 0.5208284532994026 52-53 0.5092881490601289 54-55 0.4964517078088663 56-57 0.4912671597609111 58-59 0.4892526817543262 60-61 0.4853486546097815 62-63 0.4839275887291673 64-65 0.500371351061989 66-67 0.47309000937591156 68-69 0.5001214933247382 70-71 0.48110107307651706 72-73 0.49011156772612596 74-75 0.5045564681609408 76-77 0.490814292612144 78-79 0.4901584160518605 80-81 0.4929068511616198 82-83 0.4930161639216671 84-85 0.4934534149618561 86-87 0.49487448084247027 88-89 0.5079139315052492 90-91 0.5335087334648835 92-93 0.5209065338422936 94-95 0.4920323490812418 96-97 0.46404828250914637 98-99 0.4544287596249886 100-101 0.4654537322811824 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3201822.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.81876183950933 #Duplication Level Percentage of deduplicated Percentage of total 1 65.02052787243869 25.24016386159376 2 15.040288260666282 11.67690735976745 3 6.689448110622489 7.7902827913203 4 3.7576745588370137 5.834730950795093 5 2.3640170945101393 4.5884108288158965 6 1.5307211748180858 3.5652420436774324 7 1.044302906801584 2.8376982079206603 8 0.76147590203919 2.3647641350227886 9 0.5824851185494525 2.03502159828266 >10 2.920036087948873 19.890919568329068 >50 0.18365699809942607 4.924023712207271 >100 0.09875016708570228 6.848358516522183 >500 0.004679431217526424 1.2509089146430685 >1k 0.0019363163654951099 1.15256751110245 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.56161085781783E-5 0.0 16-17 0.0 0.0 0.0 7.80805428908915E-5 0.0 18-19 0.0 0.0 0.0 1.0931276004724809E-4 0.0 20-21 0.0 0.0 0.0 1.8739330293813958E-4 0.0 22-23 0.0 0.0 0.0 4.3725104018899237E-4 0.0 24-25 0.0 0.0 0.0 5.934121259707753E-4 0.0 26-27 0.0 0.0 0.0 6.871087774398452E-4 0.0 28-29 0.0 0.0 0.0 0.001358601446301512 0.0 30-31 0.0 0.0 0.0 0.0032481505842610866 0.0 32-33 0.0 0.0 0.0 0.006777391122929383 0.0 34-35 0.0 0.0 0.0 0.011774545867946438 0.0 36-37 0.0 0.0 0.0 0.020441486128835396 0.0 38-39 0.0 0.0 0.0 0.03638553298715544 0.0 40-41 0.0 0.0 0.0 0.05465638002362405 0.0 42-43 0.0 0.0 0.0 0.07273983375715451 0.0 44-45 0.0 0.0 0.0 0.09388404477200793 0.0 46-47 0.0 0.0 0.0 0.11855749632552966 0.0 48-49 0.0 0.0 0.0 0.14577637357729442 0.0 50-51 0.0 0.0 0.0 0.17788309281402903 0.0 52-53 0.0 0.0 0.0 0.210286518113749 0.0 54-55 0.0 0.0 0.0 0.24456387644285038 0.0 56-57 0.0 0.0 0.0 0.28426002444857956 3.12322171563566E-5 58-59 0.0 0.0 0.0 0.3321702455664306 3.12322171563566E-5 60-61 3.12322171563566E-5 0.0 0.0 0.3849370764520951 3.12322171563566E-5 62-63 3.12322171563566E-5 0.0 0.0 0.4371885757546797 3.12322171563566E-5 64-65 3.12322171563566E-5 0.0 0.0 0.49432791704223406 3.12322171563566E-5 66-67 3.12322171563566E-5 0.0 0.0 0.5621330604886843 3.12322171563566E-5 68-69 3.12322171563566E-5 0.0 0.0 0.6408070155055465 3.12322171563566E-5 70-71 3.12322171563566E-5 0.0 0.0 0.7241970353130187 3.12322171563566E-5 72-73 3.12322171563566E-5 0.0 0.0 0.8092579787383558 3.12322171563566E-5 74-75 3.12322171563566E-5 0.0 0.0 0.898691432565583 3.12322171563566E-5 76-77 3.12322171563566E-5 0.0 0.0 1.002241848547483 3.12322171563566E-5 78-79 3.12322171563566E-5 0.0 0.0 1.1213927569989837 3.12322171563566E-5 80-81 3.12322171563566E-5 0.0 0.0 1.2507566004606128 3.12322171563566E-5 82-83 3.12322171563566E-5 0.0 0.0 1.3875224793882983 3.12322171563566E-5 84-85 3.12322171563566E-5 0.0 0.0 1.5271304900772122 3.12322171563566E-5 86-87 3.12322171563566E-5 0.0 0.0 1.6802464346862505 3.12322171563566E-5 88-89 3.12322171563566E-5 0.0 0.0 1.8563649072309454 3.12322171563566E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3700 0.0 40.433514 1 GTATCAA 6045 0.0 36.026134 1 GTACATG 11860 0.0 30.49754 1 TACATGG 11815 0.0 30.16444 2 ATCAACG 7180 0.0 29.860302 3 TCAACGC 7285 0.0 29.42946 4 CAACGCA 7320 0.0 29.418056 5 ACATGGG 11920 0.0 28.666943 3 AACGCAG 7635 0.0 28.202143 6 TATCAAC 7900 0.0 27.319439 2 CATGGGG 8230 0.0 24.84263 4 ACGCAGA 9305 0.0 22.734095 7 GAGTACA 8385 0.0 22.189404 1 CGCAGAG 9960 0.0 21.240026 8 GTATAGC 1160 0.0 20.814613 1 AGTACAT 8415 0.0 20.191767 2 GTACCTG 2995 0.0 20.075346 1 TCTATAC 775 0.0 19.541986 3 GTATAGG 745 0.0 19.06431 1 GCAGAGT 11040 0.0 18.176224 9 >>END_MODULE