FastQCFastQC Report
Thu 26 May 2016
SRR936311_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936311_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3201822
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36470.11390389596923252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39450.039.7932171
GTATCAA65550.038.6809041
ATCAACG79900.031.4645523
TCAACGC81900.030.6966674
CAACGCA82450.030.4918965
AACGCAG84750.029.7203586
TATCAAC86500.029.1807562
GTACATG120000.025.9261881
TACATGG118100.025.7920632
ACATGGG117150.024.5370753
ACGCAGA105200.023.8978797
CGCAGAG108900.023.0423648
CATGGGG81450.021.1404764
GAGTACA90900.020.4099941
TGCACCG10100.019.725465
GCAGAGT126750.019.3482559
AGTACAT90500.018.821182
AGAGTAC107550.018.12705410-11
GCACCGT11650.017.5082116
GACGGGT1901.8866298E-417.4760658
Produced by FastQC (version 0.11.5)