##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936311_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3201822 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50177836244488 34.0 31.0 34.0 31.0 34.0 2 32.63605159812132 34.0 31.0 34.0 31.0 34.0 3 32.722447094185746 34.0 31.0 34.0 31.0 34.0 4 36.20575128786047 37.0 35.0 37.0 35.0 37.0 5 36.16117448127972 37.0 35.0 37.0 35.0 37.0 6 36.19509360607804 37.0 37.0 37.0 35.0 37.0 7 36.146223618926975 37.0 37.0 37.0 35.0 37.0 8 36.120577283809034 37.0 36.0 37.0 35.0 37.0 9 37.90093109485787 39.0 38.0 39.0 35.0 39.0 10-11 37.83134649583893 39.0 38.0 39.0 35.0 39.0 12-13 37.779209306451136 39.0 38.0 39.0 35.0 39.0 14-15 39.15672373417385 41.0 39.0 41.0 36.0 41.0 16-17 39.07627079206776 40.0 38.0 41.0 36.0 41.0 18-19 39.073563739645735 40.0 38.5 41.0 36.0 41.0 20-21 39.01801443053361 40.0 38.5 41.0 35.0 41.0 22-23 38.90412318361233 40.0 38.0 41.0 35.0 41.0 24-25 39.05358636426385 40.0 39.0 41.0 35.0 41.0 26-27 38.97759275812334 40.0 38.5 41.0 35.0 41.0 28-29 38.890451280552135 40.0 38.0 41.0 35.0 41.0 30-31 38.79091732769655 40.0 38.0 41.0 35.0 41.0 32-33 38.655485064441436 40.0 38.0 41.0 34.5 41.0 34-35 38.52806324024259 40.0 38.0 41.0 34.0 41.0 36-37 38.39069692194007 40.0 38.0 41.0 34.0 41.0 38-39 38.260648468278376 40.0 38.0 41.0 34.0 41.0 40-41 38.122545225812054 40.0 38.0 41.0 33.0 41.0 42-43 38.16958547352101 40.0 38.0 41.0 33.0 41.0 44-45 38.06235933790198 40.0 38.0 41.0 33.0 41.0 46-47 37.89332089666446 40.0 37.0 41.0 33.0 41.0 48-49 37.69091582855012 40.0 37.0 41.0 33.0 41.0 50-51 37.48424818743828 40.0 36.5 41.0 33.0 41.0 52-53 37.25641697133695 40.0 36.0 41.0 32.0 41.0 54-55 37.039125535398284 39.0 35.5 41.0 32.0 41.0 56-57 36.76264530008227 39.0 35.0 41.0 31.0 41.0 58-59 36.477975665105674 39.0 35.0 41.0 31.0 41.0 60-61 36.17363707289162 38.0 35.0 40.0 31.0 41.0 62-63 35.85423049751048 37.5 35.0 40.0 30.0 41.0 64-65 35.47310515700123 37.0 35.0 40.0 30.0 41.0 66-67 35.11432225151805 36.0 34.0 39.0 29.0 41.0 68-69 34.75523857978364 36.0 34.0 39.0 29.0 41.0 70-71 34.385522368201606 35.0 34.0 38.5 29.0 40.0 72-73 33.99140520616074 35.0 34.0 37.5 29.0 40.0 74-75 33.58137054464614 35.0 33.5 37.0 28.0 39.0 76-77 32.875138436802544 35.0 32.5 36.0 27.0 39.0 78-79 32.94708669001587 35.0 33.0 36.0 27.0 38.5 80-81 32.804143547017915 35.0 33.0 36.0 27.0 37.0 82-83 32.55196135200521 35.0 33.0 35.0 27.0 37.0 84-85 32.29497111332235 35.0 33.0 35.0 26.5 36.5 86-87 31.92165913657911 35.0 33.0 35.0 26.0 36.0 88-89 31.45082518640949 35.0 32.0 35.0 24.5 36.0 90-91 31.254887998146057 34.5 32.0 35.0 24.0 35.0 92-93 30.988792631195615 34.0 32.0 35.0 23.5 35.0 94-95 30.844637209688734 34.0 32.0 35.0 23.0 35.0 96-97 30.608277099726344 34.0 31.0 35.0 21.5 35.0 98-99 30.168639918146603 34.0 31.0 35.0 10.0 35.0 100-101 26.785254302081754 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16020564653736358 1101 2 0.18206055188873194 1101 3 0.22302877234393037 1101 4 0.15479494879329536 1101 5 0.18003364172916037 1101 6 0.1793531404060218 1101 7 0.22835572629230683 1101 8 0.16272669136459683 1101 9 0.27907393276065307 1101 10-11 0.22039664903265077 1101 12-13 0.2966886512883633 1101 14-15 0.36208055909878567 1101 16-17 0.43050578526213457 1101 18-19 0.3620664734572472 1101 20-21 0.3328979586514791 1101 22-23 0.36789378644085247 1101 24-25 0.2966353572434741 1101 26-27 0.3996206621766376 1101 28-29 0.39743691834658534 1101 30-31 0.3787358988872285 1101 32-33 0.4066013965788713 1101 34-35 0.3974186749862554 1101 36-37 0.31409458579744154 1101 38-39 0.32830284654600206 1101 40-41 0.3592041447716099 1101 42-43 0.3418035345857362 1101 44-45 0.30385485663835254 1101 46-47 0.4157185635462639 1101 48-49 0.42561239001284434 1101 50-51 0.36582203749529896 1101 52-53 0.4392093502974532 1101 54-55 0.5160195998782129 1101 56-57 0.4501490529849903 1101 58-59 0.37746422996278284 1101 60-61 0.4226987231524788 1101 62-63 0.45365537793203004 1101 64-65 0.5528813798741083 1101 66-67 0.47275211338373424 1101 68-69 0.4451396031781485 1101 70-71 0.4868641185966922 1101 72-73 0.5735308508018022 1101 74-75 0.4440097275993651 1101 76-77 0.38049451403821166 1101 78-79 0.42674389064566753 1101 80-81 0.4568783864177064 1101 82-83 0.4310897741576767 1101 84-85 0.5820042422931806 1101 86-87 0.49499077757307575 1101 88-89 0.4904174959930998 1101 90-91 0.6492993685869628 1101 92-93 0.7120396378881857 1101 94-95 0.6654304895369414 1101 96-97 0.6047452080032301 1101 98-99 0.8185138654995008 1101 100-101 1.282687826977984 1102 1 -0.016140565103555105 1102 2 0.050009925462134674 1102 3 0.07749126711975407 1102 4 0.06624425722306171 1102 5 0.0947647313015878 1102 6 0.12681275363378575 1102 7 0.10664412917747512 1102 8 0.1328070881042649 1102 9 0.06606780223103925 1102 10-11 0.1523647037944258 1102 12-13 0.18142074176889622 1102 14-15 0.19543404508991813 1102 16-17 0.20838130379175368 1102 18-19 0.2555619764643282 1102 20-21 0.2036451241877586 1102 22-23 0.19288643581858622 1102 24-25 0.14272165686798388 1102 26-27 0.25378099724266434 1102 28-29 0.12370137180455032 1102 30-31 0.22171378662751096 1102 32-33 0.29821281047155423 1102 34-35 0.1580885961878451 1102 36-37 0.1085685187792933 1102 38-39 0.11319780260691203 1102 40-41 0.10060281037497987 1102 42-43 0.11370054699868604 1102 44-45 0.07169441666570009 1102 46-47 0.04070185462843057 1102 48-49 -0.0052706100770905095 1102 50-51 -0.02843937497215876 1102 52-53 0.02699530382121651 1102 54-55 0.10559833731008439 1102 56-57 0.15110732162482066 1102 58-59 -0.09077574536983946 1102 60-61 -0.019471500729444813 1102 62-63 0.04621783674544133 1102 64-65 0.1073659232628259 1102 66-67 0.0826995260102592 1102 68-69 -0.013491979728733838 1102 70-71 0.03984905694712637 1102 72-73 -0.1051960643720804 1102 74-75 -0.08358487936718717 1102 76-77 -0.16293123127064035 1102 78-79 -0.08964900574420653 1102 80-81 0.023825667455390942 1102 82-83 0.003387943771393509 1102 84-85 0.007565964547559645 1102 86-87 -0.06765806520664697 1102 88-89 -0.0035241465088162727 1102 90-91 0.23063083147598107 1102 92-93 0.2105944385180898 1102 94-95 0.21800126799224628 1102 96-97 0.09978654785083663 1102 98-99 0.21424227865145795 1102 100-101 0.615864169154758 1103 1 -0.06347332334129163 1103 2 0.010967856024059586 1103 3 0.06751581250165373 1103 4 0.03338905861688346 1103 5 0.07196728764553484 1103 6 0.071840787970892 1103 7 0.11490738453313298 1103 8 0.12089203865799902 1103 9 0.11107438059473651 1103 10-11 0.09495890086596859 1103 12-13 0.13734394322664656 1103 14-15 0.16482643834959276 1103 16-17 0.17946293024262872 1103 18-19 0.15818061195250976 1103 20-21 0.17449325176082198 1103 22-23 0.22807876250811177 1103 24-25 0.2259948065104993 1103 26-27 0.1264845946103108 1103 28-29 0.20908060456159916 1103 30-31 0.23018096811169642 1103 32-33 0.16910101855800264 1103 34-35 0.22882239677061733 1103 36-37 0.20311763187996235 1103 38-39 0.31841317716465767 1103 40-41 0.291548704198739 1103 42-43 0.2441473283195279 1103 44-45 0.29996854472577894 1103 46-47 0.3842902623623985 1103 48-49 0.286180663155605 1103 50-51 0.3147451445115621 1103 52-53 0.27347013772618567 1103 54-55 0.3274173634102411 1103 56-57 0.18813554975780988 1103 58-59 0.19709326119341597 1103 60-61 0.20674096626076022 1103 62-63 0.22317544263633948 1103 64-65 0.2200867986665358 1103 66-67 0.26725153975718285 1103 68-69 0.20420447928060526 1103 70-71 0.0803816558695516 1103 72-73 0.11972004654712265 1103 74-75 0.11012065533014237 1103 76-77 -0.01822484777247979 1103 78-79 -0.010760991453643953 1103 80-81 0.10059690210508876 1103 82-83 0.19289364470531467 1103 84-85 0.15929191199238346 1103 86-87 0.04733280184748523 1103 88-89 0.14482534801739178 1103 90-91 0.22046837640926142 1103 92-93 0.27744451915102175 1103 94-95 0.2700982590593881 1103 96-97 0.1573507852105358 1103 98-99 0.1210018540874529 1103 100-101 0.31388751400887926 1104 1 -0.07844187797007152 1104 2 0.0669613840738279 1104 3 0.029163308185466974 1104 4 0.049371800082930406 1104 5 -0.005526257451990091 1104 6 -0.06413091894398804 1104 7 -0.05407746775576072 1104 8 -0.003053247325951247 1104 9 -0.07917677942795365 1104 10-11 -0.0122476529135227 1104 12-13 0.06515070219919039 1104 14-15 0.08291814290878108 1104 16-17 0.09973333873799106 1104 18-19 0.16428123094123492 1104 20-21 0.04435125940441509 1104 22-23 0.0832022347961967 1104 24-25 0.11504518419590681 1104 26-27 0.1574696762690948 1104 28-29 0.13199558582455495 1104 30-31 0.029811186664005618 1104 32-33 -0.012291814563013759 1104 34-35 0.0381772784746488 1104 36-37 -0.011027670841791348 1104 38-39 0.05701364892271954 1104 40-41 0.11528786724663576 1104 42-43 0.03923106382505637 1104 44-45 0.03583765511158532 1104 46-47 0.005344557933391059 1104 48-49 0.1055060057381283 1104 50-51 0.1381904661408342 1104 52-53 0.17768465436015646 1104 54-55 0.062452373313441 1104 56-57 0.043758366481974065 1104 58-59 0.12237850124139982 1104 60-61 0.03619763859968117 1104 62-63 0.029119222218078278 1104 64-65 -0.13393817785404138 1104 66-67 -0.09237068184512509 1104 68-69 -0.01405088379022601 1104 70-71 -0.06670612150528399 1104 72-73 -0.13561388810725816 1104 74-75 -0.010863899397818955 1104 76-77 -0.012204198283761514 1104 78-79 -0.04490827791492791 1104 80-81 0.05672495249207543 1104 82-83 0.04131021760930054 1104 84-85 0.020834951391307754 1104 86-87 -0.009277545213944194 1104 88-89 0.06721370842566898 1104 90-91 0.25081928133544196 1104 92-93 0.22255444621760034 1104 94-95 0.1941566801886907 1104 96-97 0.05236973922187005 1104 98-99 -0.03221133902029294 1104 100-101 0.07655880526833769 1105 1 -0.02698099291888667 1105 2 0.04047745738196795 1105 3 0.10966188425710754 1105 4 0.036714058662077775 1105 5 0.01466764658550801 1105 6 0.03695064215943944 1105 7 0.0460964778677635 1105 8 0.0812118489265643 1105 9 0.12083390829072016 1105 10-11 0.040321272364316485 1105 12-13 0.09279802909868096 1105 14-15 0.04679851731517459 1105 16-17 0.12615157799037746 1105 18-19 0.08017500942806066 1105 20-21 0.06149820292702657 1105 22-23 -0.00967259940906473 1105 24-25 0.1462218599911722 1105 26-27 0.17486297771139192 1105 28-29 0.017238007901781316 1105 30-31 0.03285310353636817 1105 32-33 0.12749664065935207 1105 34-35 0.06140000993131167 1105 36-37 0.0707503092154056 1105 38-39 0.14917120510979487 1105 40-41 0.22627670338408734 1105 42-43 0.2631844629699245 1105 44-45 0.11979707060144307 1105 46-47 0.11677237110006189 1105 48-49 0.07925258551804859 1105 50-51 0.07035083235731321 1105 52-53 0.057143099249053364 1105 54-55 0.010303031058462864 1105 56-57 0.10811218280813506 1105 58-59 0.06573551367577579 1105 60-61 0.052623761545667946 1105 62-63 0.11998872817527939 1105 64-65 0.04234517398265325 1105 66-67 0.09781114747201514 1105 68-69 0.06398723533941109 1105 70-71 0.013358941483211595 1105 72-73 -0.015609781141208146 1105 74-75 0.04238928004837561 1105 76-77 -0.06131180379646395 1105 78-79 -0.07405825002479105 1105 80-81 0.0579920475881508 1105 82-83 0.10873080876022811 1105 84-85 0.19766824039262332 1105 86-87 0.18637645610580833 1105 88-89 0.1938492446099005 1105 90-91 0.3469134005083099 1105 92-93 0.3409591180716127 1105 94-95 0.13795104848147588 1105 96-97 0.3006121799125374 1105 98-99 0.3100342707590933 1105 100-101 0.4969617802969033 1106 1 -0.025715914691865294 1106 2 -0.004614527163958826 1106 3 -0.013054026284152087 1106 4 -0.009594770282440379 1106 5 0.01117040759982757 1106 6 -0.01190862206959764 1106 7 0.10162770646471841 1106 8 0.026801569919648216 1106 9 -0.015648922184901437 1106 10-11 0.043420038059501564 1106 12-13 0.10899732027908016 1106 14-15 0.12361994010084487 1106 16-17 0.086367319541381 1106 18-19 0.08847472229356868 1106 20-21 0.15198039685360243 1106 22-23 6.798008368491537E-4 1106 24-25 0.027192113811096874 1106 26-27 -0.033745924305655706 1106 28-29 -0.019528084610477947 1106 30-31 0.00797613416978038 1106 32-33 0.09084133701193764 1106 34-35 0.14786504773815778 1106 36-37 0.15406405113623833 1106 38-39 0.1693956150015552 1106 40-41 -0.009119502572751514 1106 42-43 0.044296370722094025 1106 44-45 0.10627303835421031 1106 46-47 0.19429100757441375 1106 48-49 0.1635162818057836 1106 50-51 0.15613297768343415 1106 52-53 0.10496155536783647 1106 54-55 0.12479049106504903 1106 56-57 0.2673205362197919 1106 58-59 0.30982520843240735 1106 60-61 0.23971287176787115 1106 62-63 0.16850668937603075 1106 64-65 0.22214730988198284 1106 66-67 0.19812318940817164 1106 68-69 0.22631491970090423 1106 70-71 0.13203105845791896 1106 72-73 8.574884826870743E-4 1106 74-75 0.20088002637120184 1106 76-77 0.08542793311034558 1106 78-79 -0.08632797359339861 1106 80-81 -0.006023064608768891 1106 82-83 0.13538314963614084 1106 84-85 0.13257233527280476 1106 86-87 0.09819476374230973 1106 88-89 0.20378403843147908 1106 90-91 0.3515114248231974 1106 92-93 0.42684586550072723 1106 94-95 0.35465870817382594 1106 96-97 0.27468317460865066 1106 98-99 0.20606035323045901 1106 100-101 0.489107972562671 1107 1 -0.02754902474035248 1107 2 0.08284372191488387 1107 3 0.03499570184355605 1107 4 0.0519432023580606 1107 5 0.06196143807193977 1107 6 0.0012852544062127436 1107 7 0.05104323538219546 1107 8 0.026252974941634477 1107 9 0.04650036589603701 1107 10-11 0.009496630615672075 1107 12-13 0.027900180499422333 1107 14-15 0.06841798857455927 1107 16-17 0.05136386573458651 1107 18-19 0.09295170890150217 1107 20-21 0.06291901275515954 1107 22-23 0.041825260460761626 1107 24-25 0.07434703000819809 1107 26-27 0.1773477365790299 1107 28-29 0.20254667483564504 1107 30-31 -0.06834212450948485 1107 32-33 0.06542282427299284 1107 34-35 0.15492820808008645 1107 36-37 0.03175371363325041 1107 38-39 0.1241875419581433 1107 40-41 0.12731422865919484 1107 42-43 -0.018922510311398355 1107 44-45 -0.016359682325642666 1107 46-47 -0.09266681584156089 1107 48-49 -0.05402086125207006 1107 50-51 0.013795578391587071 1107 52-53 0.04152398898867915 1107 54-55 0.04088765593289878 1107 56-57 -0.014283886659960388 1107 58-59 0.014612954024833869 1107 60-61 0.15606342730391276 1107 62-63 0.16922170264768255 1107 64-65 0.17205080399713069 1107 66-67 0.11514987152916234 1107 68-69 0.14874392431880068 1107 70-71 0.2554444398267677 1107 72-73 0.07304748632510183 1107 74-75 0.010962466147013572 1107 76-77 -0.11108572907891556 1107 78-79 -0.12956495157098402 1107 80-81 0.09563927852735077 1107 82-83 0.34544568611854487 1107 84-85 0.22510885112602352 1107 86-87 0.17973680066877407 1107 88-89 0.3153470156296656 1107 90-91 -0.025372488799849435 1107 92-93 0.20418278811705548 1107 94-95 0.21375718933510868 1107 96-97 0.04954138454992929 1107 98-99 0.04529693648727218 1107 100-101 0.27425819225154413 1108 1 -0.10796960724119486 1108 2 -0.03778715183638326 1108 3 -0.0028770967623970023 1108 4 0.011469421042292538 1108 5 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66-67 -0.6752055866464062 2316 68-69 -0.6605691804437654 2316 70-71 -0.5883679969307494 2316 72-73 -0.6931378129725942 2316 74-75 -0.7085665545328297 2316 76-77 -0.6037150598155492 2316 78-79 -0.8039881060641108 2316 80-81 -0.8612685069671784 2316 82-83 -0.9261835737788076 2316 84-85 -0.7458754371999774 2316 86-87 -0.8736752073868566 2316 88-89 -0.9180015762746265 2316 90-91 -0.9787578400406467 2316 92-93 -0.9026908240603788 2316 94-95 -0.8325038197709489 2316 96-97 -0.7968089745675648 2316 98-99 -0.9501785146163577 2316 100-101 -0.9869307959141835 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 25.0 5 66.0 6 97.0 7 229.0 8 562.0 9 880.0 10 1543.0 11 2320.0 12 3355.0 13 4810.0 14 6045.0 15 7405.0 16 9296.0 17 12322.0 18 11757.0 19 8839.0 20 8710.0 21 9513.0 22 10649.0 23 12097.0 24 14508.0 25 18284.0 26 22772.0 27 28922.0 28 36318.0 29 45541.0 30 56727.0 31 71108.0 32 89764.0 33 117236.0 34 165714.0 35 253525.0 36 447800.0 37 806276.0 38 790914.0 39 125832.0 40 52.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.66251474292042 17.630810339179558 12.739042009258835 24.967632908641182 2 16.59311995101745 18.951186139937647 38.17699319617386 26.27870071287104 3 20.12219917284596 22.27031983664301 28.15212713261387 29.45535385789716 4 13.479106583688912 14.710405512861117 34.62116257555854 37.18932532789143 5 14.88436896242202 36.39727630080623 33.27730273575483 15.441052001016923 6 35.93007356436429 33.67741866974491 15.892451235577742 14.500056530313051 7 31.44487732297423 30.56450358577085 19.174676168756413 18.8159429224985 8 28.12270638405258 34.45572552128132 18.435815607488486 18.985752487177614 9 27.115092594154206 15.299351431778533 18.749105977783902 38.83644999628337 10-11 26.852820486633437 24.93561072947855 26.057271587720027 22.154297196167985 12-13 26.73406480284101 21.884150558768226 26.918538037512736 24.463246600878033 14-15 23.422726185278258 23.649612626810608 24.329163207698617 28.598497980212517 16-17 24.233052621485943 26.80560515872241 24.956520557729938 24.00482166206171 18-19 24.161352504917513 25.639823200665123 26.45976259767095 23.739061696746415 20-21 24.6659438484899 24.91778502816914 26.454848563449623 23.96142255989134 22-23 24.300780711976092 24.736845064105708 26.374834820559922 24.587539403358278 24-25 24.134390402238278 25.10595389110287 26.485169890870498 24.274485815788353 26-27 24.014813808418385 25.40794582949753 26.39454921812107 24.182691143963016 28-29 23.902349591535625 25.548403559555595 26.40838691864569 24.140859930263094 30-31 24.027760166057526 25.272801208826834 26.36672328097291 24.33271534414273 32-33 23.947938212349566 25.188922698286653 26.395613679610282 24.467525409753506 34-35 24.28111927622432 25.365427293160604 26.288246281621813 24.065207148993267 36-37 24.54469645389185 25.118885508866835 26.1859817228951 24.150436314346216 38-39 24.16981110566455 25.301996538527426 26.171971441836224 24.356220913971804 40-41 24.286799389967953 25.23115910773912 26.274962919283816 24.207078583009114 42-43 24.260889323726985 25.25229936260474 26.077813288398904 24.40899802526937 44-45 24.060439462620128 25.333894704753718 26.286471833719954 24.3191939989062 46-47 24.30748941308988 25.575778911393567 25.924302772666234 24.19242890285032 48-49 24.45468877534605 25.508139255567286 25.841640539562068 24.195531429524603 50-51 24.35443457858104 25.62115584274422 25.81184103507858 24.212568543596163 52-53 24.591979122824416 25.45536465169959 25.744050012767122 24.208606212708872 54-55 24.327884386697967 25.444533508609112 25.847492895818508 24.380089208874413 56-57 24.202096467881233 25.673977896557737 25.788931787725872 24.334993847835154 58-59 24.36312369418319 25.384009111183325 25.842941401804843 24.409925792828645 60-61 24.25701082788493 25.56636083153134 26.044688586456378 24.131939754127355 62-63 24.136038792912284 25.562398534334513 26.07215516665199 24.229407506101214 64-65 23.98051964986466 25.585012510968475 25.941750404968722 24.49271743419815 66-67 24.038922213664595 25.78305414854417 25.805853667068313 24.372169970722922 68-69 24.172127619836456 25.352033935677877 26.20208119002243 24.27375725446324 70-71 24.100935859670866 25.38328645107752 25.905880585561537 24.609897103690077 72-73 24.124794956780075 25.71107676893113 25.675986737311057 24.488141536977743 74-75 23.992308129558733 25.81656631755294 25.744466744247497 24.44665880864083 76-77 24.20176982011549 25.67362614408129 25.651407555415428 24.47319648038779 78-79 24.25697617830852 25.68723753203393 25.714204422788946 24.3415818668686 80-81 24.222066267451602 25.836651016012013 25.566504663182982 24.374778053353396 82-83 24.338689033931303 25.84217673562116 25.645694857490515 24.173439372957024 84-85 24.260493635138754 25.880833206841906 25.398182521195906 24.460490636823433 86-87 24.182017607293712 25.645622919971323 25.696970360299858 24.475389112435103 88-89 24.2406361834023 25.758782554599353 25.494166957297665 24.506414304700684 90-91 24.0560019186765 25.881564335425633 25.594452129976865 24.467981615921005 92-93 24.076596931048037 25.725269751072506 25.677326186959537 24.52080713091992 94-95 24.120300023893222 25.59590160990335 25.79304410563614 24.490754260567286 96-97 24.227390227687877 25.467391920522953 25.682416331548307 24.62280152024086 98-99 24.040525628824692 25.59632660748402 25.57652479427509 24.786622969416193 100-101 23.86717219488603 25.585815101788857 25.625542761187887 24.921469942137225 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 61.0 1 50.0 2 92.0 3 160.0 4 123.0 5 103.5 6 234.5 7 473.5 8 759.0 9 835.5 10 681.0 11 605.5 12 688.0 13 794.5 14 850.5 15 935.0 16 1094.5 17 1244.5 18 1313.5 19 1508.5 20 1798.0 21 2013.5 22 2471.0 23 3194.0 24 4184.0 25 5456.5 26 7145.0 27 9394.5 28 12105.0 29 15485.5 30 19649.0 31 25096.5 32 31781.5 33 35514.5 34 37816.0 35 42193.0 36 48413.5 37 54693.0 38 60294.0 39 66004.0 40 72332.0 41 79697.5 42 86060.5 43 92891.0 44 99605.5 45 106877.0 46 113147.5 47 119249.5 48 125643.0 49 127223.0 50 126830.5 51 128144.5 52 131570.5 53 135442.0 54 138584.0 55 135396.5 56 126459.5 57 117216.0 58 111397.0 59 103065.5 60 88619.0 61 74837.5 62 63096.0 63 49865.0 64 37758.0 65 29029.0 66 20715.0 67 16111.5 68 12502.5 69 8885.5 70 6574.5 71 4780.0 72 3920.5 73 3424.0 74 2681.0 75 1995.5 76 1323.5 77 727.0 78 402.5 79 209.0 80 114.5 81 62.0 82 34.5 83 16.5 84 9.0 85 6.5 86 5.5 87 3.0 88 1.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27371915115830925 2 0.0213003721006352 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.3424162867267452E-4 12-13 7.80805428908915E-5 14-15 0.0 16-17 6.714926688616669E-4 18-19 0.0 20-21 7.80805428908915E-5 22-23 3.12322171563566E-5 24-25 0.0023580323953049233 26-27 0.00248296126393035 28-29 0.0023111840695703885 30-31 0.001249288686254264 32-33 0.0015459947492396518 34-35 0.0017646202693341478 36-37 6.24644343127132E-5 38-39 1.717771943599613E-4 40-41 7.183409945962018E-4 42-43 0.0037322499501846133 44-45 0.004341278184733567 46-47 0.014460516543393106 48-49 0.015147625320832952 50-51 0.0065587656028348856 52-53 0.0074801160089474055 54-55 3.279382801417443E-4 56-57 0.0032325344756829084 58-59 0.0013273692291451554 60-61 0.0013273692291451554 62-63 0.0 64-65 9.838148404252328E-4 66-67 0.0 68-69 0.0 70-71 2.030094115163179E-4 72-73 0.0018114685950686828 74-75 0.0 76-77 0.1967473519764684 78-79 0.10792292638379024 80-81 0.0012180564690979073 82-83 0.0 84-85 7.495732117525583E-4 86-87 0.12491325251684823 88-89 0.5962854899491602 90-91 0.3135246119240857 92-93 0.29754933284860935 94-95 0.0024048807210394585 96-97 0.0028889800869629856 98-99 0.0029670606298538766 100-101 7.027248860180235E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3201822.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.8076560881203 #Duplication Level Percentage of deduplicated Percentage of total 1 62.21579687137191 22.90017654489808 2 16.175963451377946 11.907985992246457 3 7.321154757396597 8.084236394344794 4 3.9605690276119687 5.831170507264096 5 2.5373701308668535 4.669732357260798 6 1.6812837700665244 3.713046887708818 7 1.1366636745436398 2.9286547934322975 8 0.8476476424763589 2.495993832654062 9 0.6243815173994518 2.068381814419595 >10 3.184718647298817 20.623264999489187 >50 0.1974430952800546 5.028444510310045 >100 0.10945062752586011 7.123979249930692 >500 0.005519001584026399 1.414552481127263 >1k 0.002037785199952755 1.2103796349137492 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3647 0.11390389596923252 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.12322171563566E-5 0.0 16-17 0.0 0.0 0.0 7.80805428908915E-5 0.0 18-19 0.0 0.0 0.0 9.36966514690698E-5 3.12322171563566E-5 20-21 0.0 0.0 0.0 1.56161085781783E-4 3.12322171563566E-5 22-23 0.0 0.0 0.0 4.060188230326358E-4 3.12322171563566E-5 24-25 0.0 0.0 0.0 5.621799088144188E-4 3.12322171563566E-5 26-27 0.0 0.0 0.0 6.402604517053102E-4 3.12322171563566E-5 28-29 0.0 0.0 0.0 0.001296137011988799 3.12322171563566E-5 30-31 0.0 0.0 0.0 0.0031856861499483732 6.24644343127132E-5 32-33 0.0 0.0 0.0 0.0066212300371476 6.24644343127132E-5 34-35 0.0 0.0 0.0 0.011618384782164655 6.24644343127132E-5 36-37 0.0 0.0 0.0 0.020503950563148108 6.24644343127132E-5 38-39 0.0 0.0 0.0 0.03665100683298447 6.24644343127132E-5 40-41 0.0 0.0 0.0 0.054984318303765795 6.24644343127132E-5 42-43 0.0 0.0 0.0 0.07320831701449987 6.24644343127132E-5 44-45 0.0 0.0 0.0 0.09429006359504058 1.249288686254264E-4 46-47 0.0 0.0 0.0 0.11916652456007859 1.249288686254264E-4 48-49 0.0 0.0 0.0 0.14661964344051603 1.249288686254264E-4 50-51 0.0 0.0 0.0 0.1790543009573924 1.249288686254264E-4 52-53 0.0 0.0 0.0 0.21156703901715962 1.249288686254264E-4 54-55 0.0 0.0 0.0 0.24579754902052645 1.249288686254264E-4 56-57 0.0 0.0 0.0 0.2856186258948811 1.249288686254264E-4 58-59 0.0 0.0 0.0 0.3339660980529211 1.249288686254264E-4 60-61 0.0 0.0 0.0 0.38720141219593096 1.249288686254264E-4 62-63 0.0 0.0 0.0 0.4396871531271882 1.249288686254264E-4 64-65 0.0 0.0 0.0 0.4973105937806661 1.249288686254264E-4 66-67 0.0 0.0 0.0 0.5651313533356945 1.249288686254264E-4 68-69 0.0 0.0 0.0 0.6437740761354005 1.249288686254264E-4 70-71 0.0 0.0 0.0 0.7267893093369963 1.249288686254264E-4 72-73 0.0 0.0 0.0 0.8124436648883042 1.249288686254264E-4 74-75 0.0 0.0 0.0 0.9024861469500802 1.249288686254264E-4 76-77 0.0 0.0 0.0 1.006598742840795 1.249288686254264E-4 78-79 0.0 0.0 0.0 1.126061973463859 1.249288686254264E-4 80-81 0.0 0.0 0.0 1.2559099162914116 1.249288686254264E-4 82-83 0.0 0.0 0.0 1.3925196341333153 1.249288686254264E-4 84-85 0.0 0.0 0.0 1.5324868153195275 1.249288686254264E-4 86-87 0.0 0.0 0.0 1.6856652243628782 1.405449772036047E-4 88-89 0.0 0.0 0.0 1.860971659261508 1.56161085781783E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3945 0.0 39.793217 1 GTATCAA 6555 0.0 38.680904 1 ATCAACG 7990 0.0 31.464552 3 TCAACGC 8190 0.0 30.696667 4 CAACGCA 8245 0.0 30.491896 5 AACGCAG 8475 0.0 29.720358 6 TATCAAC 8650 0.0 29.180756 2 GTACATG 12000 0.0 25.926188 1 TACATGG 11810 0.0 25.792063 2 ACATGGG 11715 0.0 24.537075 3 ACGCAGA 10520 0.0 23.897879 7 CGCAGAG 10890 0.0 23.042364 8 CATGGGG 8145 0.0 21.140476 4 GAGTACA 9090 0.0 20.409994 1 TGCACCG 1010 0.0 19.72546 5 GCAGAGT 12675 0.0 19.348255 9 AGTACAT 9050 0.0 18.82118 2 AGAGTAC 10755 0.0 18.127054 10-11 GCACCGT 1165 0.0 17.508211 6 GACGGGT 190 1.8866298E-4 17.476065 8 >>END_MODULE