FastQCFastQC Report
Thu 26 May 2016
SRR936310_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936310_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3049187
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37320.12239328056954198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38050.039.071671
GTATCAA66900.037.79221
ATCAACG80400.030.5571523
TCAACGC80500.030.5181884
CAACGCA80500.030.4005835
AACGCAG85100.028.9788556
TATCAAC86150.028.5716512
GTACATG118850.026.7307011
TACATGG116750.026.4754642
ACATGGG118300.025.2891273
ACGCAGA99600.024.712527
GTGTAGC15600.023.9767041
CGCAGAG104350.023.4972728
CATGGGG74300.022.2343224
TACCTGG23850.021.434962
TATACCG1805.3311214E-621.0380155
GAGTACA93450.019.8099981
GTACCTG26850.019.5733721
CCTATAC5600.019.4420193
GCAGAGT124650.019.4047839
Produced by FastQC (version 0.11.5)